Cited by 1733D Printing in Biomedical ResearchLiver physiology and pathologyTissue Engineering and Regenerative Medicine

Multi-cellular 3D human primary liver cell culture elevates metabolic activity under fluidic flow

Open access
Esch, Mandy B., Prot, Jean-Matthieu, Wang, Ying I., Miller, Paula, Llamas-Vidales, Jose Ricardo, Naughton, Brian A., Applegate, Dawn R., Shuler, Michael L.·2015·Lab on a Chip

Abstract

We have developed a low-cost liver cell culture device that creates fluidic flow over a 3D primary liver cell culture that consists of multiple liver cell types, including hepatocytes and non-parenchymal cells (fibroblasts, stellate cells, and Kupffer cells).

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Crossref · 10.1039/c5lc00237k

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Authors
Esch, M. B., Prot, J.-M., Wang, Y. I., Miller, P., Llamas-Vidales, J. R., Naughton, B. A., Applegate, D. R., & Shuler, M. L.
Affiliations
  • Cornell University (US)
  • RegeneMed Inc.
  • RegeneMed (United States) (US)
Corresponding author
Shuler, Michael L.
Venue
Lab on a Chip
Published
2015
Volume
15
Issue
10
Pages
2269-2277
ISSN
  • 1473-0189 (ISSN-L)
  • 1473-0197
OpenAlex
W2172247815
Cited by
173
References
29
Citations by year
2026: 6 · 2025: 12 · 2024: 13 · 2023: 5 · 2022: 14 · 2021: 14 · 2020: 22 · 2019: 19 · 2018: 31 · 2017: 14 · 2016: 19 · 2015: 3
FWCI
11.11
Type
journal article
Language
en
Open access
Locations
  • Lab on a Chip (publishedVersion)
  • PubMed (publishedVersion)
  • Europe PMC (PubMed Central) (acceptedVersion)
  • PubMed Central (acceptedVersion)
Funders
  • National Institutes of Health (UH2TR---516-01)
  • Directorate for Biological Sciences (CBET-1106153)
Topics
  • Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
  • Health Sciences › Medicine › Hepatology › Liver physiology and pathology
  • Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
Keywords
primary hepatocytes, non-parenchymal liver cells, shear stress, shear sensing, albumin synthesis, urea synthesis, CYP1A1, CYP3A4, interleukin-8, lipopolysaccharide response, hepatic metabolism
MeSH terms
Cells, Cultured, Humans, Lipopolysaccharides, Liver, Interleukin-8, Cytochrome P-450 CYP1A1, Hepatocytes, Cytochrome P-450 CYP3A, Lab-On-A-Chip Devices, Primary Cell Culture

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