Multi-cellular 3D human primary liver cell culture elevates metabolic activity under fluidic flow
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We have developed a low-cost liver cell culture device that creates fluidic flow over a 3D primary liver cell culture that consists of multiple liver cell types, including hepatocytes and non-parenchymal cells (fibroblasts, stellate cells, and Kupffer cells).
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- Authors
- Esch, M. B., Prot, J.-M., Wang, Y. I., Miller, P., Llamas-Vidales, J. R., Naughton, B. A., Applegate, D. R., & Shuler, M. L.
- Affiliations
- Cornell University (US)
- RegeneMed Inc.
- RegeneMed (United States) (US)
- Corresponding author
- Shuler, Michael L.
- Venue
- Lab on a Chip
- Publisher
- Royal Society of Chemistry (RSC)
- Published
- 2015
- Volume
- 15
- Issue
- 10
- Pages
- 2269-2277
- ISSN
- 1473-0189 (ISSN-L)
- 1473-0197
- PMC
- PMC4422761
- OpenAlex
- W2172247815
- Cited by
- 173
- References
- 29
- Citations by year
- 2026: 6 · 2025: 12 · 2024: 13 · 2023: 5 · 2022: 14 · 2021: 14 · 2020: 22 · 2019: 19 · 2018: 31 · 2017: 14 · 2016: 19 · 2015: 3
- FWCI
- 11.11
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Lab on a Chip (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institutes of Health (UH2TR---516-01)
- Directorate for Biological Sciences (CBET-1106153)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Health Sciences › Medicine › Hepatology › Liver physiology and pathology
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Keywords
- primary hepatocytes, non-parenchymal liver cells, shear stress, shear sensing, albumin synthesis, urea synthesis, CYP1A1, CYP3A4, interleukin-8, lipopolysaccharide response, hepatic metabolism
- MeSH terms
- Cells, Cultured, Humans, Lipopolysaccharides, Liver, Interleukin-8, Cytochrome P-450 CYP1A1, Hepatocytes, Cytochrome P-450 CYP3A, Lab-On-A-Chip Devices, Primary Cell Culture
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