Expanding the structural analysis capabilities on an Orbitrap-based mass spectrometer for large macromolecular complexes
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Native mass spectrometry can provide insight into the structure of macromolecular biological systems.
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Instrument hardware improvements enhanced large ion transmission and detection, especially with UHMR Orbitraps capable of analyzing species at 70,000 m/z and mass as large as 800 kDa.
“More recently, instrument hardware improvements have led to enhanced large ion transmission and detection, especially with the implementation of ultra-high mass range (UHMR) Orbitraps that are capable of analyzing species at 70,000 m/z and mass as large as 800 kDa [103,104].”
From Top-down proteomics: challenges, innovations, and applications in basic and clinical research (2020) · cites this paper as [103]
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- Authors
- Fort, K. L., van de Waterbeemd, M., Boll, D., Reinhardt-Szyba, M., Belov, M. E., Sasaki, E., Zschoche, R., Hilvert, D., Makarov, A. A., & Heck, A. J. R.
- Affiliations
- Utrecht University (NL)
- Biomolecular Mass Spectrometry and Proteomics
- Thermo Fisher Scientific (Germany) (DE)
- ETH Zurich (CH)
- Corresponding author
- Heck, Albert J. R.
- Venue
- The Analyst
- Publisher
- Royal Society of Chemistry (RSC)
- Published
- 2018
- Volume
- 143
- Issue
- 1
- Pages
- 100-105
- ISSN
- 0003-2654 (ISSN-L)
- 1364-5528
- OpenAlex
- W2767559908
- Cited by
- 122
- References
- 16
- Citations by year
- 2026: 10 · 2025: 11 · 2024: 11 · 2023: 12 · 2022: 13 · 2021: 20 · 2020: 18 · 2019: 18 · 2018: 8
- FWCI
- 5.93
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- The Analyst (publishedVersion)
- PubMed (publishedVersion)
- Utrecht University Repository (Utrecht University) (submittedVersion, other-oa)
- Zenodo (CERN European Organization for Nuclear Research) (submittedVersion, other-oa)
- Utrecht University Repository (Utrecht University) (submittedVersion)
- Funders
- Stichting voor Fundamenteel Onderzoek der Materie (12PR3303-2)
- Nederlandse Organisatie voor Wetenschappelijk Onderzoek (184.032.201, 718.015.003)
- H2020 Future and Emerging Technologies (686547)
- Topics
- Physical Sciences › Chemistry › Spectroscopy › Mass Spectrometry Techniques and Applications
- Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolomics and Mass Spectrometry Studies
- Keywords
- native mass spectrometry, macromolecular complexes, signal intensity enhancement, mass resolution
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