In vitro and in vivo evaluation of an exopolysaccharide produced by Lactobacillus helveticus KLDS1.8701 for the alleviative effect on oxidative stress
Abstract
Correlations between oxidative stress and degenerative diseases have been gaining increasing attention. A number of studies affirm that exopolysaccharide (EPS) produced by lactic acid bacteria (LAB) can alleviate oxidative stress and further prevent the related diseases. In our previous study, Lactobacillus helveticus KLDS1.8701 has been shown to possess high antioxidant capacity in vitro. The aim of this study was to evaluate the ameliorative effects of EPS produced by L. helveticus KLDS1.8701 on oxidative stress. Firstly, EPS was isolated from the culture of L. helveticus KLDS1.8701 and purified using DEAE-Sepharose Fast Flow chromatography. Secondly, the antioxidant capacities of EPS fractions were evaluated using in vitro methods. Thirdly, an in vivo study was performed to investigate the possible protective effects of EPS on d-galactose (d-gal)-induced liver damage and gut microbiota disorder. In vitro antioxidant activity results suggested that EPS-1 exhibited strong scavenging properties on 2,2-diphenyl-1-picrylhydrazyl radical, superoxide radical, hydroxyl radical, and chelating activity on ferrous ion. In vivo, EPS-1 supplementation significantly attenuated oxidative status such as decreased organic index, liver injury and liver oxidative stress. EPS-1 supplementation shifted the gut microbiota composition to that of the control group. In addition, the analysis of Spearman's rank correlation suggested that the protective effects of EPS correlated with manipulating the gut microbiota composition in d-gal-induced mice. These results implied that EPS-1 supplementation could mitigate hepatic oxidative stress via manipulating the gut microbiota composition and be used as a potential candidate to attenuate oxidative damage.
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Intake of EPS-1 produced by Lb. helveticus KLDS1.8701 significantly alleviated liver injury and oxidative stress in an aging mice model, together with a decrease in oxidative-stress related bacteria in gut microbiota, confirming correlation of antioxidant activity of EPS with gut microbiota modulation.
“A similar result in the aging mice model has been found after the intake of EPS-1 produced by Lb. helveticus KLDS1.8701 that significantly alleviated liver injury and oxidative stress, together with a decrease in oxidative-stress related bacteria in the gut microbiota, confirming the correlation of antioxidant activity of EPS with gut microbiota modulation [130].”
From Lactic Acid Bacteria Exopolysaccharides Producers: A Sustainable Tool for Functional Foods (2021) · cites this paper as [130]
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- Authors
- Li, B., Du, P., Smith, E. E., Wang, S., Jiao, Y., Guo, L., Huo, G., & Liu, F.
- Affiliations
- Northeast Agricultural University (CN)
- Heilongjiang University of Chinese Medicine (CN)
- Corresponding author
- Huo, Guicheng
- Liu, Fei
- Venue
- Food & Function
- Publisher
- Royal Society of Chemistry (RSC)
- Published
- 2019
- Volume
- 10
- Issue
- 3
- Pages
- 1707-1717
- ISSN
- 2042-6496 (ISSN-L)
- 2042-650X
- OpenAlex
- W2917329365
- Cited by
- 56
- References
- 63
- Citations by year
- 2026: 3 · 2025: 9 · 2024: 8 · 2023: 6 · 2022: 11 · 2021: 9 · 2020: 8 · 2019: 2
- FWCI
- 5.79
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Food & Function (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Natural Science Foundation of Heilongjiang Province (QC2016039)
- Northeast Agricultural University
- National Natural Science Foundation of China (31801518)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Agricultural and Biological Sciences › Food Science › Polysaccharides Composition and Applications
- Health Sciences › Nursing › Nutrition and Dietetics › Microbial Metabolites in Food Biotechnology
- Keywords
- exopolysaccharides, Lactobacillus helveticus, oxidative stress, antioxidant activity, hepatic oxidative stress, gut microbiota, lactic acid bacteria, free radical scavenging, ferrous ion chelation, dietary supplementation
- MeSH terms
- Animals, Biphenyl Compounds, Iron, Iron Chelating Agents, Liver, Male, Mice, Inbred BALB C, Picrates, Polysaccharides, Bacterial, Specific Pathogen-Free Organisms, Superoxides, Free Radical Scavengers, Hydroxyl Radical, Oxidative Stress, Mice, Lactobacillus helveticus
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