Arg82p is a bifunctional protein whose inositol polyphosphate kinase activity is essential for nitrogen and PHO gene expression but not for Mcm1p chaperoning in yeast
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Summary: In Saccharomyces cerevisiae, the synthesis of inositol pyrophosphates is essential for vacuole biogenesis and the cell's response to certain environmental stresses. The kinase activity of Arg82p and Kcs1p is required for the production of soluble inositol phosphates. To define physiologically relevant targets of the catalytic products of Arg82p and Kcs1p, we used DNA microarray technology. In arg82Δ or kcs1Δ cells, we observed a derepressed expression of genes regulated by phosphate (PHO) on high phosphate medium and a strong decrease in the expression of genes regulated by the quality of nitrogen source (NCR). Arg82p and Kcs1p are required for activation of NCR‐regulated genes in response to nitrogen availability, mainly through Nil1p, and for repression of PHO genes by phosphate. Only the catalytic activity of both kinases was required for PHO gene repression by phosphate and for NCR gene activation in response to nitrogen availability, indicating a role for inositol pyrophosphates in these controls. Arg82p also controls expression of arginine‐responsive genes by interacting with Arg80p and Mcm1p, and expression of Mcm1‐dependent genes by interacting with Mcm1p. We show here that Mcm1p and Arg80p chaperoning by Arg82p does not involve the inositol polyphosphate kinase activity of Arg82p, but requires its polyaspartate domain. Our results indicate that Arg82p is a bifunctional protein whose inositol kinase activity plays a role in multiple signalling cascades, and whose acidic domain protects two MADS‐box proteins against degradation.
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Several further studies have supported the importance of Arg82 kinase activity in transcriptional regulation and chromatin remodelling, together suggesting that Ins(1,4,5,6)P4 has an important regulatory role in yeast.
“Several further studies have supported the importance of Arg82 kinase activity in transcriptional regulation and chromatin remodelling 37-39, together suggesting that Ins(1,4,5,6)P4 has an important regulatory role in yeast.”
From Structure of Hdac3 Bound to Co-Repressor and Inositol Tetraphosphate (2012) · cites this paper as [38]
The Abstract reports that Arg82p kinase activity is required for regulation of nitrogen-responsive and PHO genes. The Discussion reports a role for Ins(1,4,5,6)P4 in chromatin remodelling, citing Steger et al. (2003), but this paper does not show that Arg82p kinase activity itself regulates chromatin remodelling; its own transcriptional findings point chiefly to inositol pyrophosphates. (LLM, conf=0.83)
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- Authors
- El Alami, M., Messenguy, F., Scherens, B., & Dubois, E.
- Affiliations
- Université Libre de Bruxelles (BE)
- Corresponding author
- Dubois, Evelyne
- Venue
- Molecular Microbiology
- Publisher
- Wiley
- Published
- 2003-06-17
- Volume
- 49
- Issue
- 2
- Pages
- 457-468
- ISSN
- 0950-382X (ISSN-L)
- 1365-2958
- OpenAlex
- W2102745796
- Cited by
- 83
- References
- 38
- Citations by year
- 2026: 1 · 2025: 3 · 2024: 4 · 2023: 1 · 2021: 1 · 2019: 2 · 2017: 6 · 2016: 5 · 2015: 3 · 2014: 1 · 2013: 6 · 2012: 9
- FWCI
- 1.96
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Molecular Microbiology (publishedVersion)
- PubMed (publishedVersion)
- Dépôt institutionnel de l'Université libre de Bruxelles (Université Libre de Bruxelles) (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biochemical and Molecular Research
- Keywords
- inositol polyphosphate kinase, inositol pyrophosphates, bifunctional protein, MADS-box transcription factors, nitrogen catabolite repression, Saccharomyces cerevisiae, gene expression regulation
- MeSH terms
- Inositol Phosphates, Nitrogen, Saccharomyces cerevisiae, Transcriptional Activation, Gene Expression Regulation, Fungal, Phosphotransferases (Alcohol Group Acceptor), Phosphotransferases (Phosphate Group Acceptor), Genes, Reporter, Oligonucleotide Array Sequence Analysis, Two-Hybrid System Techniques, Saccharomyces cerevisiae Proteins
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