Cited by 355Gastrointestinal motility and disordersGut microbiota and healthInflammatory Bowel Disease

Altered 5-Hydroxytryptamine Signaling in Patients With Constipation- and Diarrhea-Predominant Irritable Bowel Syndrome

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Atkinson, Wendy, Lockhart, Stephen, Whorwell, Peter J., Keevil, Brian, Houghton, Lesley A.·2006·Gastroenterology

Abstract

Background & aims Evidence suggests that postprandial platelet-depleted plasma 5-hydroxytryptamine (5-HT) concentrations may be abnormal in irritable bowel syndrome (IBS). However, interpretation of the data has been hampered by the variable methodology and rather small numbers used in previous studies. Therefore, the aim of this study was to measure concentrations of platelet-depleted plasma 5-HT and its metabolite 5-HIAA under fasting and fed conditions in a large group of patients with diarrhea-predominant (d-) and constipation-predominant (c-) IBS, compared with controls. The ratio of plasma 5-HIAA:5-HT and platelet stores was also assessed.Methods Twenty-nine c-IBS patients (aged, 19-53 years), 55 d-IBS patients (aged, 19-52 years), and 35 healthy volunteers (aged, 18-46 years) had platelet-depleted plasma 5-HT/5-HIAA concentrations measured using reverse-phase, high-performance liquid chromatography with fluorimetric detection before and after a standard meal.Results d-IBS patients had raised platelet-depleted plasma 5-HT concentrations under fasting and fed conditions (P < .05). However, the postprandial relative to fasting concentration was similar to controls. In contrast, c-IBS patients failed to show an increase in platelet-depleted plasma 5-HT concentration with meal ingestion compared with controls (P < .01). c-IBS was associated with decreased 5-HIAA (P < .01) but normal 5-HIAA:5-HT ratio and d-IBS with normal 5-HIAA concentrations but reduced 5-HIAA:5-HT ratio (P < .005). C-IBS but not d-IBS patients had increased platelet 5-HT.Conclusions These results support the concept that d-IBS is characterized by reduced 5-HT reuptake, whereas impaired release may be a feature of c-IBS. These results also provide a rational basis for current pharmacologic approaches involving modulation of different 5-HT receptors in c- and d-IBS.

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Crossref · 10.1053/j.gastro.2005.09.031

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  • Changes in gastrointestinal motility may be influenced by alterations in serotonin metabolism, with high levels reported in patients with IBS-D, and low levels in IBS-C.

    “Changes in gastrointestinal motility may be influenced by alterations in serotonin (5-Hydroxytryptamine (5-HT)) metabolism, 49 with high levels reported in patients with IBS-D, and low levels in IBS-C.50”

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Research works printed but not linked: 4

  1. Gershon MD. Importance of serotonergic mechanisms in gastro- intestinal motility and sensation. In: Camilleri M, Spiller RC, eds. Irritable bowel syndrome-diagnosis and treatment. Edinburgh: W. B. Saunders, 2002:95-115.
  2. Whitaker RP, Dursun SM, Davies T. Measurement of platelet rich plasma 5-HT and platelet poor plasma 5-HIAA and 5-HT in normal adults (abstr). Ann Clin Biochem 1996;537:341.
  3. Bose M, Nickols C, Greenwald S, Feakins R, Perrett D, Farthing M. 5-hydroxytryptamine (5-HT) levels in colonic mucosa in the irritable bowel syndrome (IBS): assessment by high performance liquid chromatography (HPLC) (abstr). Gut 2001;48:A57.
  4. Houghton LA. Sensory dysfunction and the irritable bowel syn- drome. In: Houghton LA, Whorwell PJ, eds. Irritable bowel syn- drome. Baillieres clinical gastroenterology. Vol. 13. No 3. Lon- don: Baillieres Tindall, 1999:415-427.

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Authors
Atkinson, W., Lockhart, S., Whorwell, P. J., Keevil, B., & Houghton, L. A.
Corresponding author
Houghton, Lesley A.
Venue
Gastroenterology
Publisher
Elsevier BV
Published
2006-01
Volume
130
Issue
1
Pages
34-43
ISSN
0016-5085 (ISSN-L)
OpenAlex
W2128077096
Cited by
355
References
41
Citations by year
2026: 10 · 2025: 11 · 2024: 15 · 2023: 21 · 2022: 20 · 2021: 30 · 2020: 18 · 2019: 12 · 2018: 11 · 2017: 15 · 2016: 15 · 2015: 12 · 2014: 30 · 2013: 16 · 2012: 20
FWCI
18.47
Type
journal article
Language
en
Open access
Locations
  • Gastroenterology (publishedVersion)
  • PubMed (publishedVersion)
  • Research Explorer (The University of Manchester) (publishedVersion, other-oa)
Topics
  • Health Sciences › Medicine › Gastroenterology › Gastrointestinal motility and disorders
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Inflammatory Bowel Disease
Keywords
irritable bowel syndrome, irritable bowel syndrome with constipation, diarrhea-predominant irritable bowel syndrome, serotonin, 5-hydroxyindoleacetic acid, serotonin uptake, serotonin release, serotonin receptors
MeSH terms
Adult, Chromatography, High Pressure Liquid, Constipation, Diarrhea, Fasting, Female, Humans, Hydroxyindoleacetic Acid, Male, Middle Aged, Serotonin, Signal Transduction, Case-Control Studies, Postprandial Period, Irritable Bowel Syndrome

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