The DOI matches its Crossref record; 28 of the 28 references it cites were found in a registry or an index.
High-throughput size-based rare cell enrichment using microscale vortices
Open accessAbstract
Cell isolation in designated regions or from heterogeneous samples is often required for many microfluidic cell-based assays. However, current techniques have either limited throughput or are incapable of viable off-chip collection. We present an innovative approach, allowing high-throughput and label-free cell isolation and enrichment from heterogeneous solution using cell size as a biomarker. The approach utilizes the irreversible migration of particles into microscale vortices, developed in parallel expansion-contraction trapping reservoirs, as the cell isolation mechanism. We empirically determined the critical particle/cell diameter Dcrt and the operational flow rate above which trapping of cells/particles in microvortices is initiated. Using this approach we successfully separated larger cancer cells spiked in blood from the smaller blood cells with processing rates as high as 7.5×106 cells/s. Viable long-term culture was established using cells collected off-chip, suggesting that the proposed technique would be useful for clinical and research applications in which in vitro culture is often desired. The presented technology improves on current technology by enriching cells based on size without clogging mechanical filters, employing only a simple single-layered microfluidic device and processing cell solutions at the ml/min scale.
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Rapid Prototyping of Microfluidic Systems in Poly(dimethylsiloxane)
Duffy, D. C., McDonald, J. C., Schueller, O. J. A., & Whitesides, G. M. (1998) · Analytical Chemistry · doi:10.1021/ac980656z · Cited by 5,288
Circulating Tumor Cells, Disease Progression, and Survival in Metastatic Breast Cancer
Cristofanilli, M., Budd, G. T., Ellis, M. J., Stopeck, A., Matera, J., Miller, M. C., Reuben, J. M., Doyle, G. V., Allard, W. J., Terstappen, L. W. M. M., et al. (2004) · New England Journal of Medicine · doi:10.1056/nejmoa040766 · Cited by 4,593
Isolation of rare circulating tumour cells in cancer patients by microchip technology
Nagrath, S., Sequist, L. V., Maheswaran, S., Bell, D. W., Irimia, D., Ulkus, L., Smith, M. R., Kwak, E. L., Digumarthy, S., Muzikansky, A., et al. (2007) · Nature · doi:10.1038/nature06385 · Cited by 3,559
Laflamme, M. A., Chen, K. Y., Naumova, A. V., Muskheli, V., Fugate, J. A., Dupras, S. K., Reinecke, H., Xu, C., Hassanipour, M., Police, S., et al. (2007) · Nature Biotechnology · doi:10.1038/nbt1327 · Cited by 2,206
Continuous inertial focusing, ordering, and separation of particles in microchannels
Di Carlo, D., Irimia, D., Tompkins, R. G., & Toner, M. (2007) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0704958104 · Cited by 1,708
Self-citationViscous and resistive eddies near a sharp corner
Moffatt, H. K. (1964) · Journal of Fluid Mechanics · doi:10.1017/s0022112064000015 · Cited by 1,531
Label-free cell separation and sorting in microfluidic systems
Gossett, D. R., Weaver, W. M., Mach, A. J., Hur, S. C., Tse, H. T. K., Lee, W., Amini, H., & Di Carlo, D. (2010) · Analytical and Bioanalytical Chemistry · doi:10.1007/s00216-010-3721-9 · Cited by 930
Self-citationYamada, M., Nakashima, M., & Seki, M. (2004) · Analytical Chemistry · doi:10.1021/ac049863r · Cited by 746
Deterministic hydrodynamics: Taking blood apart
Davis, J. A., Inglis, D. W., Morton, K. J., Lawrence, D. A., Huang, L. R., Chou, S. Y., Sturm, J. C., & Austin, R. H. (2006) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0605967103 · Cited by 622
Zheng, S., Lin, H., Liu, J.-Q., Balic, M., Datar, R., Cote, R. J., & Tai, Y.-C. (2007) · Journal of Chromatography A · doi:10.1016/j.chroma.2007.05.064 · Cited by 599
Cited by
Fundamentals and applications of inertial microfluidics: a review
Zhang, J., Yan, S., Yuan, D., Alici, G., Nguyen, N.-T., Ebrahimi Warkiani, M., & Li, W. (2016) · Lab on a Chip · doi:10.1039/c5lc01159k · Cited by 1,017
Hydrodynamic mechanisms of cell and particle trapping in microfluidics
Karimi, A., Yazdi, S., & Ardekani, A. M. (2013) · Biomicrofluidics · doi:10.1063/1.4799787 · Cited by 384
Abdulla, A., Liu, W., Gholamipour-Shirazi, A., Sun, J., & Ding, X. (2018) · Analytical Chemistry · doi:10.1021/acs.analchem.7b04210 · Cited by 131
Microfluidic platform for negative enrichment of circulating tumor cells
Sajay, B. N. G., Chang, C.-P., Ahmad, H., Khuntontong, P., Wong, C. C., Wang, Z., Puiu, P. D., Soo, R., & Rahman, A. R. A. (2014) · Biomedical Microdevices · doi:10.1007/s10544-014-9856-2 · Cited by 56
Isolation of circulating tumour cells by physical means in a microfluidic device: a review
Patil, P., Madhuprasad, M., Kumeria, T., Losic, D., & Kurkuri, M. (2015) · RSC Advances · doi:10.1039/c5ra16489c · Cited by 47
Metadata
- Authors
- Hur, S. C., Mach, A. J., & Di Carlo, D.
- Venue
- Biomicrofluidics
- Publisher
- AIP Publishing
- Published
- 2011-06-01
- Volume
- 5
- Issue
- 2
- Pages
- 22206-22206
- ISSN
- 1932-1058 (ISSN-L)
- OpenAlex
- W2037714118
- Cited by
- 317
- References
- 28
- FWCI
- 16.96
- Type
- journal article
- Language
- en
- Open access
- bronze
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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