Cited by 43Optical Imaging and Spectroscopy TechniquesSpectroscopy Techniques in Biomedical and Chemical ResearchOptical Coherence Tomography Applications

The role of tissue fluorescence in in vivo optical bioimaging

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Lifante, José, Shen, Yingli, Ximendes, Erving, Martín Rodríguez, Emma, Ortgies, Dirk H.·2020·Journal of Applied Physics

Abstract

The technological advancements made in optics and semiconductors (e.g., cameras and laser diodes) working with infrared have brought interest in optical bioimaging back to the forefront of research investigating in vivo medical imaging techniques. The definition of the near-infrared transparency windows has turned optical imaging into more than just a method for topical imaging applications. Moreover, this has focused attention back to tissue fluorescence, emissions by tissues and organs that occur when excited by external illumination sources. Most endogenous fluorophores emit in the blue to green range of the electromagnetic spectrum and the resulting tissue fluorescence can be employed in studies from cells to tissue metabolism or avoided by shifting to the red if seen as unwanted autofluorescence. With the more recent move to infrared, it was discovered that autofluorescence is not limited to the visible but also strongly affects in vivo imaging in the infrared. In this Tutorial, we give an overview on tissue fluorescence and tissue interactions with excitation light as well as their effect on in vivo imaging. Furthermore, potential sources of tissue fluorescence in the near-infrared are identified and we describe approaches for successful biomedical imaging in the biological windows, taking into consideration infrared autofluorescence and summarizing techniques for avoiding it in in vivo imaging experiments.

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Crossref · 10.1063/5.0021854

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Authors
Lifante, J., Shen, Y., Ximendes, E., Martín Rodríguez, E., & Ortgies, D. H.
Affiliations
  • Instituto Ramón y Cajal de Investigación Sanitaria (ES)
  • Universidad Autónoma de Madrid (ES)
  • Nanobiology Group, Instituto Ramón y Cajal de Investigación Sanitaria 1 , IRYCIS, Ctra. Colmenar km. 9.100, 28034 Madrid, Spain
Corresponding author
Ortgies, Dirk H.
Venue
Journal of Applied Physics
Published
2020-11-04
Volume
128
Issue
17
ISSN
  • 0021-8979 (ISSN-L)
  • 1089-7550
OpenAlex
W3094875912
Cited by
43
References
142
Citations by year
2026: 3 · 2025: 11 · 2024: 8 · 2023: 6 · 2022: 11 · 2021: 4
FWCI
2.57
Type
journal article
Language
en
Open access
Licence
cc-by
Locations
  • Journal of Applied Physics (publishedVersion, cc-by)
  • Biblos-e Archivo (Universidad Autónoma de Madrid) (publishedVersion)
  • Hispana (submittedVersion)
Funders
  • Horizon 2020 Framework Programme - FET Open (NanoTBTech)
  • Fundación para la Investigación Biomédica del Hospital Universitario Ramón y Cajal (IMP18_38(2018/0265))
  • European Cooperation in Science and Technology (CA17140)
  • Ministerio de Economía, Industria y Competitividad, Gobierno de España (MAT2016-75362-C3-1-R)
  • Comunidad de Madrid (B2017/BMD-3867RENIMCM)
  • Ministerio de Ciencia, Innovación y Universidades (PID2019-106211RB-I00, FJC2018-036734-I)
  • Instituto de Salud Carlos III (PI16/00812, PI19/00565, CD17/00210)
  • China Scholarship Council (201806870023)
Topics
  • Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Optical Imaging and Spectroscopy Techniques
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Spectroscopy Techniques in Biomedical and Chemical Research
  • Physical Sciences › Engineering › Biomedical Engineering › Optical Coherence Tomography Applications
Keywords
tissue fluorescence, near-infrared autofluorescence, endogenous fluorophores, biological window, excitation light, infrared imaging

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