Essential oils from Lippia turbinata and Tagetes minuta persistently reduce in vitro ruminal methane production in a continuous-culture system
Abstract
The aim of the present study was to evaluate the impact of essential oils (EO) from Lippia turbinata (LT) and Tagetes minuta (TM) as well as the rotation of both EO on fermentation parameters in vitro. Daily addition of LT, TM, or a 3-day rotation between them (TM/LT), as well as a control (without EO), was evaluated using the rumen simulation technique (Rusitec). The experiment lasted 19 days, with a 7-day adaptation period, followed by 12 days of treatment (Days 0–12). The EO were dissolved in ethanol (70% vol/vol) to be added daily to fermenters (300 µL/L) from Day 0. Daily measurements included methane concentration, total gas production, apparent DM disappearance and pH, which started 2 days before the addition of treatments. On Days 0, 4, 8 and 12 apparent crude protein disappearance and neutral detergent fibre disappearance, ammonia and volatile fatty acid concentration and composition were determined. Methane production was significantly inhibited shortly after addition of both EO added individually, and persisted over time with no apparent adaptation to EO addition. The TM/LT treatment showed a similar effect on methane production, suggesting that rotating the EO did not bring further improvements in reduction or persistency compared with the inclusion of the EO individually. Gas production, total volatile fatty acid concentration and composition and apparent crude protein disappearance were not affected by EO addition. Compared with the control, a 5% reduction of apparent DM disappearance and a 15% reduction of neutral detergent fibre disappearance were observed with the addition of EO. Only TM and TM/LT reduced ammonia concentration. Given the significant and persistent antimethanogenic activity of both EO, and the potential of T. minuta to modify nitrogen metabolism, EO from these plant species are of interest for developing new feed additives with potential application in ruminant nutrition that are also likely to be acceptable to consumers.
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Essential oils of Lippia turbinata and Tagetes minuta caused a 10-fold decrease of in vitro CH4 production coupled with modification of nitrogen metabolism in the rumen.
“Essential oils of Lippia turbinata and Tagetes minuta have shown a 10-fold decrease of in vitro CH4 production coupled with modification of N metabolism in the rumen (249).”
From Phytogenic Additives Can Modulate Rumen Microbiome to Mediate Fermentation Kinetics and Methanogenesis Through Exploiting Diet–Microbe Interaction (2020) · cites this paper as [249]
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- Authors
- Garcia, F., Vercoe, P. E., Martínez, M. J., Durmic, Z., Brunetti, M. A., Moreno, M. V., Colombatto, D., Lucini, E., & Ferrer, J. M.
- Affiliations
- AConsejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Godoy Cruz 2290, Buenos Aires, C1425FQB, Argentina.
- The University of Western Australia (AU)
- National Agricultural Technology Institute (AR)
- Consejo Nacional de Investigaciones Científicas y Técnicas (AR)
- Universidad Nacional de Córdoba (AR)
- Venue
- Animal Production Science
- Publisher
- CSIRO Publishing
- Published
- 2018-05-24
- Volume
- 59
- Issue
- 4
- Pages
- 709-720
- ISSN
- 1836-0939 (ISSN-L)
- 1836-5787
- OpenAlex
- W2804555726
- Cited by
- 14
- References
- 49
- Citations by year
- 2026: 1 · 2025: 2 · 2022: 2 · 2021: 4 · 2020: 3 · 2019: 1 · 2018: 1
- FWCI
- 3.80
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Animal Production Science (publishedVersion)
- UWA Profiles and Research Repository (UWA) (submittedVersion)
- Funders
- Australian Government
- Consejo Nacional de Investigaciones Científicas y Técnicas
- Topics
- Life Sciences › Agricultural and Biological Sciences › Agronomy and Crop Science › Ruminant Nutrition and Digestive Physiology
- Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Nutrition and Physiology
- Life Sciences › Agricultural and Biological Sciences › Food Science › Essential Oils and Antimicrobial Activity
- Keywords
- essential oils, Lippia turbinata, Tagetes minuta, rumen fermentation, volatile fatty acids, ammonia, feed additives, ruminant nutrition, RUSITEC, nitrogen metabolism
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