The reduction of methane production in the in vitro ruminal fermentation of different substrates is linked with the chemical composition of the essential oil
Open accessAbstract
There is interest in identifying natural products capable of manipulating rumen microbial activity to develop new feed additives for ruminant nutrition as a strategy to reduce methane. Two trials were performed using the in vitro gas production technique to evaluate the interaction of substrate (n = 5) and additive (n = 6, increasing doses: 0, 0.3, 3, 30, and 300 µL/L of essential oils—EO—of Lippia turbinata or Tagetes minuta, and monensin at 1.87 mg/L). The two EO utilized were selected because they differ markedly in their chemical composition, especially in the proportion of oxygenated compounds. For both EO, the interaction between the substrate and additive was significant for all variables; however, the interaction behaved differently for the two EO. Within each substrate, the response was dose-dependent, without effects at a low level of EO and a negative outcome at the highest dose. The intermediate dose (30 µL/L) inhibited methane with a slight reduction on substrate digestibility, with L. turbinata being more effective than T. minuta. It is concluded that the effectiveness of the EO to reduce methane production depends on interactions between the substrate that is fermented and the additive dose that generates different characteristics within the incubation medium (e.g., pH); and thus, the chemical nature of the compounds of the EO modulates the magnitude of this response.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Garcia et al. revealed that the chemical composition of essential oils, especially the proportion of oxygenated compounds, has a positive interaction with fermentation pattern and promising potential for CH4 mitigation.
“Recently, Garcia et al. (238) revealed that the chemical composition of EO, especially proportion of oxygenated compounds, has a positive interaction with fermentation pattern and indicate promising potential regarding CH4 mitigation.”
From Phytogenic Additives Can Modulate Rumen Microbiome to Mediate Fermentation Kinetics and Methanogenesis Through Exploiting Diet–Microbe Interaction (2020) · cites this paper as [238]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Van Soest, P. J., Robertson, J. B., & Lewis, B. A. (1991) · Journal of Dairy Science · doi:10.3168/jds.s0022-0302(91)78551-2 · Cited by 28,658 · see citing works
Essential oils: Their antibacterial properties and potential applications in foods—A review
Burt, S. (2004) · International Journal of Food Microbiology · doi:10.1016/j.ijfoodmicro.2004.03.022 · Cited by 10,488 · see citing works
Biological effects of essential oils—A review
Bakkali, F., Averbeck, S., Averbeck, D., & Idaomar, M. (2008) · Food and Chemical Toxicology · doi:10.1016/j.fct.2007.09.106 · Cited by 7,929 · see citing works
A TWO‐STAGE TECHNIQUE FOR THE IN VITRO DIGESTION OF FORAGE CROPS
Tilley, J. M. A., & Terry, R. A. (1963) · Grass and Forage Science · doi:10.1111/j.1365-2494.1963.tb00335.x · Cited by 6,624 · see citing works
Antimicrobial agents from plants: Antibacterial activity of plant volatile oils
Dorman, H. J. D., & Deans, S. G. (2000) · Journal of Applied Microbiology · doi:10.1046/j.1365-2672.2000.00969.x · Cited by 4,334 · see citing works
Johnson, K. A., & Johnson, D. E. (1995) · Journal of Animal Science · doi:10.2527/1995.7382483x · Cited by 2,714 · cited 1× · see citing works
“Methane is the main GHG emitted by the livestock sector; it has a warming potential 28 times greater than carbon dioxide [3], and for the animal, represents also a loss of ingested energy [4].”
BackgroundTheodorou, M. K., Williams, B. A., Dhanoa, M. S., McAllan, A. B., & France, J. (1994) · Animal Feed Science and Technology · doi:10.1016/0377-8401(94)90171-6 · Cited by 1,809 · see citing works
A review of plant-derived essential oils in ruminant nutrition and production
Benchaar, C., Calsamiglia, S., Chaves, A. V., Fraser, G. R., Colombatto, D., McAllister, T. A., & Beauchemin, K. A. (2007) · Animal Feed Science and Technology · doi:10.1016/j.anifeedsci.2007.04.014 · Cited by 636 · see citing works
Self-citationRumen methanogens and mitigation of methane emission by anti-methanogenic compounds and substances
Patra, A. K., Park, T., Kim, M., & Yu, Z. (2017) · Journal of Animal Science and Biotechnology/Journal of animal science and biotechnology · doi:10.1186/s40104-017-0145-9 · Cited by 469 · see citing works
A multiple-comparisons method based on the distribution of the root node distance of a binary tree
Rienzo, J. A. D., Guzman, A. W., & Casanoves, F. (2002) · Journal of Agricultural Biological and Environmental Statistics · doi:10.1198/10857110260141193 · Cited by 464 · see citing works
Manipulation of rumen fermentation and methane production with plant secondary metabolites
Bodas, R., Prieto, N., García-González, R., Andrés, S., Giráldez, F. J., & López, S. (2012) · Animal Feed Science and Technology · doi:10.1016/j.anifeedsci.2012.07.010 · Cited by 437 · see citing works
Patra, A. K., & Yu, Z. (2012) · Applied and Environmental Microbiology · doi:10.1128/aem.00309-12 · Cited by 416 · see citing works
Biochemistry of plant secondary metabolites and their effects in animals
Acamovic, T., & Brooker, J. D. (2005) · Proceedings of The Nutrition Society · doi:10.1079/pns2005449 · Cited by 395 · see citing works
Essential oils and opportunities to mitigate enteric methane emissions from ruminants
Benchaar, C., & Greathead, H. M. R. (2011) · Animal Feed Science and Technology · doi:10.1016/j.anifeedsci.2011.04.024 · Cited by 357 · see citing works
Castillejos, L., Calsamiglia, S., & Ferret, A. (2006) · Journal of Dairy Science · doi:10.3168/jds.s0022-0302(06)72341-4 · Cited by 344 · see citing works
Cardozo, P. W., Calsamiglia, S., Ferret, A., & Kamel, C. (2005) · Journal of Animal Science · doi:10.2527/2005.83112572x · Cited by 296 · see citing works
Critical evaluation of essential oils as rumen modifiers in ruminant nutrition: a review
Cobellis, G., Trabalza-Marinucci, M., & Yu, Z. (2016) · Science of The Total Environment · doi:10.1016/j.scitotenv.2015.12.103 · Cited by 291 · see citing works
Yáñez-Ruíz, D. R., Bannink, A., Dijkstra, J., Kebreab, E., Morgavi, D. P., O’Kiely, P., Reynolds, C. K., Schwarm, A., Shingfield, K. J., Yu, Z., et al. (2016) · Animal Feed Science and Technology · doi:10.1016/j.anifeedsci.2016.03.016 · Cited by 211 · see citing works
Anti-methanogenic effects of monensin in dairy and beef cattle: A meta-analysis
Appuhamy, J. A. D. R. N., Strathe, A. V., Jayasundara, S., Wagner‐Riddle, C., Dijkstra, J., France, J., & Kebreab, E. (2013) · Journal of Dairy Science · doi:10.3168/jds.2012-5923 · Cited by 175 · see citing works
Essential Oil Constituents of the GenusLippia(Verbenaceae)—A Literature Review
Terblanche, F. C., & Kornelius, G. (1996) · Journal of Essential Oil Research · doi:10.1080/10412905.1996.9700673 · Cited by 159 · see citing works
Castillejos, L., Calsamiglia, S., Ferret, A., & Losa, R. (2005) · Animal Feed Science and Technology · doi:10.1016/j.anifeedsci.2004.12.008 · Cited by 156 · see citing works
Virucidal activity of essential oils from aromatic plants of San Luis, Argentina
García, C. C., Talarico, L. B., Almeida, N. V., Colombres, S., Duschatzky, C. B., & Damonte, E. B. (2003) · Phytotherapy Research · doi:10.1002/ptr.1305 · Cited by 132 · see citing works
Cobellis, G., Trabalza‐Marinucci, M., Marcotullio, M. C., & Yu, Z. (2016) · Animal Feed Science and Technology · doi:10.1016/j.anifeedsci.2016.02.008 · Cited by 124 · see citing works
Antibiotic resistance: mitigation opportunities in livestock sector development
Robinson, T. P., Bu, D., Carrique-Mas, J. J., Fèvre, E. M., Gilbert, M., Grace, D., Hay, S. I., Jiwakanon, J., Kakkar, M., Kariuki, S., et al. (2016) · animal · doi:10.1017/s1751731116001828 · Cited by 98 · see citing works
Molero, R., Ibars, M., Calsamiglia, S., Ferret, A., & Losa, R. (2004) · Animal Feed Science and Technology · doi:10.1016/j.anifeedsci.2003.11.011 · Cited by 94 · see citing works
Patra, A. K., & Yu, Z. (2015) · Frontiers in Microbiology · doi:10.3389/fmicb.2015.00297 · Cited by 92 · see citing works
The effects of livestock methane emission on the global warming: a review
Moumen, A., Azizi, G., Chekroun, K. B., & Baghour, M. (2016) · International Journal of Global Warming · doi:10.1504/ijgw.2016.074956 · Cited by 82 · see citing works
Senatore, F., Napolitano, F., Mohamed, M. A.-H., Harris, P. J. C., Mnkeni, P. N. S., & Henderson, J. (2004) · Flavour and Fragrance Journal · doi:10.1002/ffj.1358 · Cited by 74 · see citing works
Garcia, M. V., Matias, J., Barros, J. C., de Lima, D. P., Lopes, R. D. S., & Andreotti, R. (2012) · Revista Brasileira de Parasitologia Veterinária/Brazilian Journal of Veterinary Parasitology · doi:10.1590/s1984-29612012000400011 · Cited by 63 · see citing works
Héthélyi, E., Dános, B., Tétényi, P., & Koczka, I. (1986) · Flavour and Fragrance Journal · doi:10.1002/ffj.2730010408 · Cited by 61 · see citing works
Chemical composition of essential oil from tagetes minuta L. leaves and flowers
Chamorro, E., Ballerini, G. A., Sequeira, A. F., Velasco, G. A., & Zalazar, M. F. (2008) · Cited by 55 · see citing works
Klevenhusen, F., Muro‐Reyes, A., Khiaosa‐ard, R., Metzler-Zebeli, B. U., & Zebeli, Q. (2012) · Animal Feed Science and Technology · doi:10.1016/j.anifeedsci.2012.07.008 · Cited by 49 · see citing works
Joch, M., Čermák, L., Hakl, J., Hučko, B., Dušková, D., & Marounek, M. (2015) · Asian-Australasian Journal of Animal Sciences · doi:10.5713/ajas.15.0474 · Cited by 47 · see citing works
Mateos, I., Ranilla, M. J., Tejido, M. L., Saro, C., Kamel, C., & Carro, M. D. (2013) · Animal Production Science · doi:10.1071/an12167 · Cited by 45 · see citing works
Joch, M., Kudrna, V., Hakl, J., Božík, M., Homolka, P., Illek, J., Tyrolová, Y., & Výborná, A. (2019) · Animal Feed Science and Technology · doi:10.1016/j.anifeedsci.2019.03.009 · Cited by 40 · see citing works
de Araújo, R. C., Pires, A. V., Mourão, G. B., Abdalla, A. L., & Sallam, S. M. A. (2011) · Animal Feed Science and Technology · doi:10.1016/j.anifeedsci.2011.04.009 · Cited by 39 · see citing works
In Vitro Activity of Essential Oils from San Luis-Argentina AgainstAscosphaera apis
Dellacasa, A. D., Bailac, P. N., Ponzi, M. I., Ruffinengo, S. R., & Eguaras, M. J. (2003) · Journal of Essential Oil Research · doi:10.1080/10412905.2003.9712143 · Cited by 38 · see citing works
Cattani, M., Maccarana, L., Rossi, G., Tagliapietra, F., Schiavon, S., & Bailoni, L. (2016) · Animal Feed Science and Technology · doi:10.1016/j.anifeedsci.2016.05.014 · Cited by 27 · see citing works
Kahvand, M., & Malecky, M. (2017) · Annals of Animal Science · doi:10.1515/aoas-2017-0024 · Cited by 17 · see citing works
Kouazounde, J. B., Jin, L., Assogba, F., Ayédoun, M. A., Wang, Y., Beauchemin, K. A., McAllister, T. A., & Gbénou, J. (2014) · Journal of the Science of Food and Agriculture · doi:10.1002/jsfa.6785 · Cited by 15 · see citing works
Garcia, F., Vercoe, P. E., Martínez, M. J., Durmic, Z., Brunetti, M. A., Moreno, M. V., Colombatto, D., Lucini, E., & Ferrer, J. M. (2019) · Animal Production Science · doi:10.1071/an17469 · Cited by 14 · see citing works
Self-citationAntibacterial activity of essential oils of Aloysia polystachya and Lippia turbinata (Verbenaceae)
Zamora, C. M. P., Torres, C. A., Aguado, M. I., Bela, A., Núñez, M. B., & Bregni, C. (2016) · Redalyc (Universidad Autónoma del Estado de México) · Cited by 13 · see citing works
Modificación de la fermentación microbiana ruminal mediante compuestos de aceites esenciales
Castillejos, L. (2006) · Dipòsit Digital de Documents de la UAB (Universitat Autònoma de Barcelona) · Cited by 1 · see citing works
Flagged first, then by citations
Research works printed but not linked: 1
- [43]Garcia F. Martínez Ferrer J. Durmic Z. Vercoe P.E. Effect of Ethanol as Carrier of Essential Oils on in Vitro Methane Production Proceedings of the XXIV Reunión de la Asociación Latinoamericana de Producción Animal (ALPA) Puerto Varas, Chile 9–13 November 2015 518
Cited by
Sorted by citations
Hassan, F.-U., Arshad, M. A., Ebeid, H. M., Rehman, M. S.-U., Khan, M. S., Shahid, S., & Yang, C. (2020) · Frontiers in Veterinary Science · doi:10.3389/fvets.2020.575801 · Cited by 160 · see citing works
- Authors
- Garcia, F., Colombatto, D., Brunetti, M. A., Martínez, M. J., Moreno, M. V., Scorcione Turcato, M. C., Lucini, E., Frossasco, G., & Martínez Ferrer, J.
- Affiliations
- Universidad Nacional de Córdoba (AR)
- Consejo Nacional de Investigaciones Científicas y Técnicas (AR)
- National Agricultural Technology Institute (AR)
- Consejo Nacional de Investigaciones Científicas y Técnicas, Buenos Aires C1425FQB, Argentina
- Estación Experimental Agropecuaria Manfredi, Instituto Nacional de Tecnología Agropecuaria, Córdoba 5988, Argentina
- Corresponding author
- Garcia, Florencia
- ORCID
- Venue
- Animals
- Publisher
- MDPI AG
- Published
- 2020-05-01
- Volume
- 10
- Issue
- 5
- Pages
- 786
- ISSN
- 2076-2615 (ISSN-L)
- PMC
- PMC7277337
- OpenAlex
- W3023384011
- Cited by
- 32
- References
- 44
- Citations by year
- 2026: 1 · 2025: 6 · 2024: 6 · 2023: 6 · 2022: 5 · 2021: 6 · 2020: 2
- FWCI
- 5.92
- Type
- journal article
- Language
- en
- Open access
- gold
- dx.doi.org
- Licence
- Locations
- Animals (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas) (submittedVersion, cc-by)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas) (submittedVersion)
- Conicet (submittedVersion, cc-by)
- LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas) (submittedVersion, other-oa)
- Funders
- Instituto Nacional de Tecnología Agropecuaria (PNPA 1126023)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Agronomy and Crop Science › Ruminant Nutrition and Digestive Physiology
- Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Nutrition and Physiology
- Life Sciences › Agricultural and Biological Sciences › Food Science › Essential Oils and Antimicrobial Activity
- Keywords
- methane mitigation, rumen fermentation, essential oils, Lippia turbinata, Tagetes minuta, monensin, feed additives, ruminant nutrition, dose-dependent effects, oxygenated compounds, rumen pH
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
