The DOI matches its Crossref record; 42 of the 42 references it cites were found in a registry or an index.
Human Fanconi anemia monoubiquitination pathway promotes homologous DNA repair
Open accessAbstract
Fanconi anemia (FA) is a recessive disorder characterized by congenital abnormalities, progressive bone-marrow failure, and cancer susceptibility. Cells from FA patients are hypersensitive to agents that produce DNA crosslinks and, after treatment with these agents, have pronounced chromosome breakage and other cytogenetic abnormalities. Eight FANC genes have been cloned, and the encoded proteins interact in a common cellular pathway. DNA-damaging agents activate the monoubiquitination of FANCD2, resulting in its targeting to nuclear foci that also contain BRCA1 and BRCA2/FANCD1, proteins involved in homology-directed DNA repair. Given the interaction of the FANC proteins with BRCA1 and BRCA2, we tested whether cells from FA patients (groups A, G, and D2) and mouse Fanca –/– cells with a targeted mutation are impaired for this repair pathway. We find that both the upstream (FANCA and FANCG) and downstream (FANCD2) FA pathway components promote homology-directed repair of chromosomal double-strand breaks (DSBs). The FANCD2 monoubiquitination site is critical for normal levels of repair, whereas the ATM phosphorylation site is not. The defect in these cells, however, is mild, differentiating them from BRCA1 and BRCA2 mutant cells. Surprisingly, we provide evidence that these proteins, like BRCA1 but unlike BRCA2, promote a second DSB repair pathway involving homology, i.e., single-strand annealing. These results suggest an early role for the FANC proteins in homologous DSB repair pathway choice.
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“The DR-U2OS cell line containing a single integrated copy of the DR-GFP reporter was used46,47.”
From BRCA1–BARD1 promotes RAD51-mediated homologous DNA pairing (2017) · cites this paper as [46]
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XRCC3 promotes homology-directed repair of DNA damage in mammalian cellsPreprint
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Homology-directed repair is a major double-strand break repair pathway in mammalian cells
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“Generally, HR between repeats on the same chromosome is efficient in both rodent and human cells, and can be as efficient as NHE”
Self-citationHomologous-recombination-deficient tumours are dependent on Polθ-mediated repair
Ceccaldi, R., Liu, J., Amunugama, R., Hajdú, I., Primack, B., Petalcorin, M. I. R., O’Connor, K. W., Konstantinopoulos, P. A., Elledge, S. J., Boulton, S. J., et al. (2015) · Nature · doi:10.1038/nature14184 · Cited by 945
Self-citation
Metadata
- Authors
- Nakanishi, K., Yang, Y.-G., Pierce, A. J., Taniguchi, T., Digweed, M., D'Andrea, A. D., Wang, Z.-Q., & Jasin, M.
- Affiliations
- Memorial Sloan Kettering Cancer Center (US)
- Molecular Biology Program, Memorial Sloan–Kettering Cancer Center, 1275 York Avenue, New York, NY 10021; International Agency for Research on Cancer, 150 Cours Albert Thomas, 69008 Lyon, France; Department of Radiation Oncology, Dana–Farber Cancer Institute, Harvard Medical School, 44 Binney Street, Boston, MA 02115; and Institut für Humangenetik, Charite-Campus Virchow-Klinikum, Humboldt Universität zu Berlin, Augustenburger Platz 1, 13353 Berlin, Germany
- Corresponding author
- Jasin, Maria
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 2005-01-13
- Volume
- 102
- Issue
- 4
- Pages
- 1110-1115
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC545844
- OpenAlex
- W2029164849
- Cited by
- 384
- References
- 42
- Citations by year
- 2026: 5 · 2025: 7 · 2024: 11 · 2023: 10 · 2022: 7 · 2021: 15 · 2020: 12 · 2019: 12 · 2018: 11 · 2017: 24 · 2016: 13 · 2015: 25 · 2014: 22 · 2013: 10 · 2012: 26
- FWCI
- 12.67
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Fanconi Anemia Research Fund
- National Institutes of Health
- National Cancer Institute (P01-CA94060, P01CA094060)
- National Institute of General Medical Sciences (R01GM054668, GM54668)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
- Health Sciences › Medicine › Hematology › Blood disorders and treatments
- Health Sciences › Medicine › Oncology › PARP inhibition in cancer therapy
- Keywords
- Fanconi anemia, FANCD2 monoubiquitination, homology-directed repair, DNA double-strand break repair, single-strand annealing, BRCA1, BRCA2, FANCA, FANCG, ATM phosphorylation, DNA repair pathway choice
- MeSH terms
- Cell Line, DNA Repair, Humans, Nuclear Proteins, Recombination, Genetic, Ubiquitin, Fanconi Anemia Complementation Group D2 Protein
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