The DOI matches its Crossref record; 42 of the 42 references it cites were found in a registry or an index.
Human Fanconi anemia monoubiquitination pathway promotes homologous DNA repair
Open accessAbstract
Fanconi anemia (FA) is a recessive disorder characterized by congenital abnormalities, progressive bone-marrow failure, and cancer susceptibility. Cells from FA patients are hypersensitive to agents that produce DNA crosslinks and, after treatment with these agents, have pronounced chromosome breakage and other cytogenetic abnormalities. Eight FANC genes have been cloned, and the encoded proteins interact in a common cellular pathway. DNA-damaging agents activate the monoubiquitination of FANCD2, resulting in its targeting to nuclear foci that also contain BRCA1 and BRCA2/FANCD1, proteins involved in homology-directed DNA repair. Given the interaction of the FANC proteins with BRCA1 and BRCA2, we tested whether cells from FA patients (groups A, G, and D2) and mouse Fanca –/– cells with a targeted mutation are impaired for this repair pathway. We find that both the upstream (FANCA and FANCG) and downstream (FANCD2) FA pathway components promote homology-directed repair of chromosomal double-strand breaks (DSBs). The FANCD2 monoubiquitination site is critical for normal levels of repair, whereas the ATM phosphorylation site is not. The defect in these cells, however, is mild, differentiating them from BRCA1 and BRCA2 mutant cells. Surprisingly, we provide evidence that these proteins, like BRCA1 but unlike BRCA2, promote a second DSB repair pathway involving homology, i.e., single-strand annealing. These results suggest an early role for the FANC proteins in homologous DSB repair pathway choice.
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“The DR-U2OS cell line containing a single integrated copy of the DR-GFP reporter was used46,47.”
From BRCA1–BARD1 promotes RAD51-mediated homologous DNA pairing (2017) · cites this paper as [46]
Citations
Cites
XRCC3 promotes homology-directed repair of DNA damage in mammalian cellsPreprint
Pierce, A. J., Johnson, R. D., Thompson, L. H., & Jasin, M. (1999) · Genes & Development · doi:10.1101/gad.13.20.2633 · Cited by 1,384
Self-citationBrca1 Controls Homology-Directed DNA Repair
Moynahan, M. E., Chiu, J. W., Koller, B. H., & Jasin, M. (1999) · Molecular Cell · doi:10.1016/s1097-2765(00)80202-6 · Cited by 1,245
Self-citationInteraction of the Fanconi Anemia Proteins and BRCA1 in a Common Pathway
Garcia-Higuera, I., Taniguchi, T., Ganesan, S., Meyn, M. S., Timmers, C., Hejna, J., Grompe, M., & D'Andrea, A. D. (2001) · Molecular Cell · doi:10.1016/s1097-2765(01)00173-3 · Cited by 1,198
Self-citationBiallelic Inactivation of BRCA2 in Fanconi Anemia
Howlett, N. G., Taniguchi, T., Olson, S., Cox, B., Waisfisz, Q., de Die-Smulders, C., Persky, N., Grompe, M., Joenje, H., Pals, G., et al. (2002) · Science · doi:10.1126/science.1073834 · Cited by 1,149
Self-citationBRCA2 Is Required for Homology-Directed Repair of Chromosomal Breaks
Moynahan, M. E., Pierce, A. J., & Jasin, M. (2001) · Molecular Cell · doi:10.1016/s1097-2765(01)00174-5 · Cited by 1,007
Self-citationMolecular views of recombination proteins and their control
West, S. C. (2003) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm1127 · Cited by 977
The Fanconi anaemia/BRCA pathway
D'Andrea, A. D., & Grompe, M. (2003) · Nature Reviews Cancer · doi:10.1038/nrc970 · Cited by 822
Self-citationLee, S. E., Moore, J. K., Holmes, A. M., Umezu, K., Kolodner, R. D., & Haber, J. E. (1998) · Cell · doi:10.1016/s0092-8674(00)81482-8 · Cited by 784
BRCA2 Function in DNA Binding and Recombination from a BRCA2-DSS1-ssDNA Structure
Yang, H., Jeffrey, P. D., Miller, J. J., Kinnucan, E., Sun, Y., Thomä, N. H., Zheng, N., Chen, P., Lee, W., & Pavletich, N. P. (2002) · Science · doi:10.1126/science.297.5588.1837 · Cited by 719
Homology-directed repair is a major double-strand break repair pathway in mammalian cells
Liang, F., Han, M., Romanienko, P. J., & Jasin, M. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.9.5172 · Cited by 649
Self-citationIn search of the tumour-suppressor functions of BRCA1 and BRCA2
Scully, R., & Livingston, D. M. (2000) · Nature · doi:10.1038/35044000 · Cited by 643
The emerging genetic and molecular basis of Fanconi anaemia
Joenje, H., & Patel, K. J. (2001) · Nature Reviews Genetics · doi:10.1038/35076590 · Cited by 566
Ku DNA end-binding protein modulates homologous repair of double-strand breaks in mammalian cellsPreprint
Pierce, A. J., Hu, P., Han, M., Ellis, N. A., & Jasin, M. (2001) · Genes & Development · doi:10.1101/gad.946401 · Cited by 514
Self-citationGenetic Steps of Mammalian Homologous Repair with Distinct Mutagenic Consequences
Stark, J. M., Pierce, A. J., Oh, J., Pastink, A., & Jasin, M. (2004) · Molecular and Cellular Biology · doi:10.1128/mcb.24.21.9305-9316.2004 · Cited by 501
Self-citationConvergence of the Fanconi Anemia and Ataxia Telangiectasia Signaling Pathways
Taniguchi, T., García-Higuera, I., Xu, B., Andreassen, P. R., Gregory, R. C., Kim, S., Lane, W. S., Kastan, M. B., & D’Andrea, A. D. (2002) · Cell · doi:10.1016/s0092-8674(02)00747-x · Cited by 452
Self-citationRad51 Recombinase and Recombination Mediators
Sung, P., Krejčí, L., Komen, S. V., & Sehorn, M. G. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.r300027200 · Cited by 446
Richardson, C. A., Moynahan, M. E., & Jasin, M. (1998) · Genes & Development · doi:10.1101/gad.12.24.3831 · Cited by 442
Self-citationTutt, A. N. J., Bertwistle, D., Valentine, J., Gabriel, A., Swift, S., Ross, G., Griffin, C. S., Thacker, J., & Ashworth, A. (2001) · The EMBO Journal · doi:10.1093/emboj/20.17.4704 · Cited by 429
Ivanov, E. L., Sugawara, N., Fishman‐Lobell, J., & Haber, J. E. (1996) · Genetics · doi:10.1093/genetics/142.3.693 · Cited by 390
Moynahan, M. E., Cui, T. Y., & Jasin, M. (2001) · PubMed · Cited by 382
Self-citationHomologous repair of DNA damage and tumorigenesis:the BRCA connection
Jasin, M. (2002) · Oncogene · doi:10.1038/sj.onc.1206176 · Cited by 363
Self-citationMammalian XRCC2 promotes the repair of DNA double-strand breaks by homologous recombination
Johnson, R. D., Liu, N., & Jasin, M. (1999) · Nature · doi:10.1038/43932 · Cited by 323
Self-citationThe Fanconi Anaemia Gene FANCC Promotes Homologous Recombination and Error-Prone DNA Repair
Niedźwiedź, W., Mosedale, G., Johnson, M. A., Ong, C. Y., Pace, P., & Patel, K. J. (2004) · Molecular Cell · doi:10.1016/j.molcel.2004.08.009 · Cited by 310
Structure of the single-strand annealing domain of human RAD52 protein
Singleton, M. R., Wentzell, L. M., Liu, Y., West, S. C., & Wigley, D. B. (2002) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.212449899 · Cited by 222
Grompe, M. (2001) · Human Molecular Genetics · doi:10.1093/hmg/10.20.2253 · Cited by 189
Ku80-deficient Cells Exhibit Excess Degradation of Extrachromosomal DNA
Liang, F., & Jasin, M. (1996) · Journal of Biological Chemistry · doi:10.1074/jbc.271.24.14405 · Cited by 174
Self-citationMouse RAD54 Affects DNA Double-Strand Break Repair and Sister Chromatid Exchange
Dronkert, M. L. G., Beverloo, H. B., Johnson, R. D., Hoeijmakers, J. H. J., Jasin, M., & Kanaar, R. (2000) · Molecular and Cellular Biology · doi:10.1128/mcb.20.9.3147-3156.2000 · Cited by 174
Self-citationRoles of BRCA1 in DNA damage repair: a link between development and cancer
Deng, C. (2003) · Human Molecular Genetics · doi:10.1093/hmg/ddg082 · Cited by 169
Yamamoto, K., Ishiai, M., Matsushita, N., Arakawa, H., Lamerdin, J. E., Buerstedde, J.-M., Tanimoto, M., Harada, M., Thompson, L. H., & Takata, M. (2003) · Molecular and Cellular Biology · doi:10.1128/mcb.23.15.5421-5430.2003 · Cited by 151
ATP Hydrolysis by Mammalian RAD51 Has a Key Role during Homology-directed DNA Repair
Stark, J. M., Hu, P., Pierce, A. J., Moynahan, M. E., Ellis, N. A., & Jasin, M. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.m112132200 · Cited by 144
Self-citationExtensive Loss of Heterozygosity Is Suppressed during Homologous Repair of Chromosomal Breaks
Stark, J. M., & Jasin, M. (2002) · Molecular and Cellular Biology · doi:10.1128/mcb.23.2.733-743.2003 · Cited by 122
Self-citationPapadopoulo, D., Guillouf, C., Mohrenweiser, H. W., & Moustacchi, E. (1990) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.87.21.8383 · Cited by 120
BARD1 Participates with BRCA1 in Homology-Directed Repair of Chromosome Breaks
Westermark, U., Reyngold, M., Olshen, A. B., Baer, R. J., Jasin, M., & Moynahan, M. E. (2003) · Molecular and Cellular Biology · doi:10.1128/mcb.23.21.7926-7936.2003 · Cited by 120
Self-citationMcAllister, K. A., Bennett, L. M., Houle, C. D., Ward, T., Malphurs, J., Collins, N. K., Cachafeiro, C., Haseman, J., Goulding, E. H., Bunch, D. O., et al. (2002) · PubMed · Cited by 113
Godthelp, B. C., Artwert, F., Joenje, H., & Zdzienicka, M. Z. (2002) · Oncogene · doi:10.1038/sj.onc.1205656 · Cited by 100
Donoho, G. P., Brenneman, M. A., Cui, T. X., Donoviel, D. B., Vogel, H. S., Goodwin, E. H., Chen, D. J., & Hasty, P. (2003) · Genes Chromosomes and Cancer · doi:10.1002/gcc.10148 · Cited by 97
Attenuation of the formation of DNA-repair foci containing RAD51 in Fanconi anaemia
Digweed, M. (2002) · Carcinogenesis · doi:10.1093/carcin/23.7.1121 · Cited by 87
Self-citationFanconi anemia C gene product plays a role in the fidelity of blunt DNA end-joining
Escarceller, M., Buchwald, M., Singleton, B. K., Jeggo, P. A., Jackson, S. P., Moustacchi, E., & Papadopoulo, D. (1998) · Journal of Molecular Biology · doi:10.1006/jmbi.1998.1784 · Cited by 82
Sala-Trepat, M., Boyse, J., Richard, P., Papadopoulo, D., & Moustacchi, E. (1993) · Mutation research. Fundamental and molecular mechanisms of mutagenesis · doi:10.1016/0027-5107(93)90137-5 · Cited by 49
A DNA Double Strand Break Repair Defect in Fanconi Anemia Fibroblasts
Donahue, S. L., & Campbell, C. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.m207937200 · Cited by 44
Deficient Regulation of DNA Double-strand Break Repair in Fanconi Anemia Fibroblasts
Donahue, S. L., Lundberg, R., Saplis, R., & Campbell, C. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m213251200 · Cited by 32
Immortalization of four new fanconi anemia fibroblast cell lines by an improved procedure
Jakobs, P. M., Sahaayaruban, P., Saito, H., Reifsteck, C. A., Olson, S. B., Joenje, H., Moses, R. E., & Grompe, M. (1996) · Somatic Cell and Molecular Genetics · doi:10.1007/bf02369905 · Cited by 32
Research works printed but not linked: 2
- Moynahan, M. E., Cui, T. Y. & Jasin, M. (2001) Cancer Res. 61, 4842–4850.11406561
- McAllister, K. A., Bennett, L. M., Houle, C. D., Ward, T., Malphurs, J., Collins, N. K., Cachafeiro, C., Haseman, J., Goulding, E. H., Bunch, D., et al. (2002) Cancer Res. 62, 990–994.11861370
Cited by
Sorted by citations
53BP1 Inhibits Homologous Recombination in Brca1-Deficient Cells by Blocking Resection of DNA Breaks
Bunting, S. F., Callén, E., Wong, N., Chen, H.-T., Polato, F., Gunn, A., Bothmer, A., Feldhahn, N., Fernandez-Capetillo, O., Cao, L., et al. (2010) · Cell · doi:10.1016/j.cell.2010.03.012 · Cited by 1,671
“HPRT-DRGFPhyg (Nakanishi et al., 2005) was electroporated into various MEF cell lines and integrants were selected in 90-125 μg/ml Hygromycin (Roche).”
InfluentialDouble-Strand Break End Resection and Repair Pathway Choice
Symington, L. S., & Gautier, J. (2011) · Annual Review of Genetics · doi:10.1146/annurev-genet-110410-132435 · Cited by 1,556
McCabe, N., Turner, N. C., Lord, C. J., Kluzek, K., Białkowska, A., Swift, S., Giavara, S., O’Connor, M. J., Tutt, A. N. J., Zdzienicka, M. Z., et al. (2006) · Cancer Research · doi:10.1158/0008-5472.can-06-0140 · Cited by 1,311
Mitotic homologous recombination maintains genomic stability and suppresses tumorigenesis
Moynahan, M. E., & Jasin, M. (2010) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2851 · Cited by 959
“Generally, HR between repeats on the same chromosome is efficient in both rodent and human cells, and can be as efficient as NHE”
Self-citationHomologous-recombination-deficient tumours are dependent on Polθ-mediated repair
Ceccaldi, R., Liu, J., Amunugama, R., Hajdú, I., Primack, B., Petalcorin, M. I. R., O’Connor, K. W., Konstantinopoulos, P. A., Elledge, S. J., Boulton, S. J., et al. (2015) · Nature · doi:10.1038/nature14184 · Cited by 945
Self-citation
Metadata
- Authors
- Nakanishi, K., Yang, Y.-G., Pierce, A. J., Taniguchi, T., Digweed, M., D'Andrea, A. D., Wang, Z.-Q., & Jasin, M.
- Affiliations
- Memorial Sloan Kettering Cancer Center (US)
- Molecular Biology Program, Memorial Sloan–Kettering Cancer Center, 1275 York Avenue, New York, NY 10021; International Agency for Research on Cancer, 150 Cours Albert Thomas, 69008 Lyon, France; Department of Radiation Oncology, Dana–Farber Cancer Institute, Harvard Medical School, 44 Binney Street, Boston, MA 02115; and Institut für Humangenetik, Charite-Campus Virchow-Klinikum, Humboldt Universität zu Berlin, Augustenburger Platz 1, 13353 Berlin, Germany
- Corresponding author
- Jasin, Maria
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 2005-01-13
- Volume
- 102
- Issue
- 4
- Pages
- 1110-1115
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC545844
- OpenAlex
- W2029164849
- Cited by
- 384
- References
- 42
- Citations by year
- 2026: 5 · 2025: 7 · 2024: 11 · 2023: 10 · 2022: 7 · 2021: 15 · 2020: 12 · 2019: 12 · 2018: 11 · 2017: 24 · 2016: 13 · 2015: 25 · 2014: 22 · 2013: 10 · 2012: 26
- FWCI
- 12.67
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Fanconi Anemia Research Fund
- National Institutes of Health
- National Cancer Institute (P01-CA94060, P01CA094060)
- National Institute of General Medical Sciences (R01GM054668, GM54668)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
- Health Sciences › Medicine › Hematology › Blood disorders and treatments
- Health Sciences › Medicine › Oncology › PARP inhibition in cancer therapy
- Keywords
- Fanconi anemia, FANCD2 monoubiquitination, homology-directed repair, DNA double-strand break repair, single-strand annealing, BRCA1, BRCA2, FANCA, FANCG, ATM phosphorylation, DNA repair pathway choice
- MeSH terms
- Cell Line, DNA Repair, Humans, Nuclear Proteins, Recombination, Genetic, Ubiquitin, Fanconi Anemia Complementation Group D2 Protein
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
