The DOI matches its Crossref record; 9 of the 9 references it cites were found in a registry or an index.
MicroRNAs control translation initiation by inhibiting eukaryotic initiation factor 4E/cap and poly(A) tail function
Open accessAbstract
MicroRNAs (miRNAs) repress translation of target mRNAs by interaction with partially mismatched sequences in their 3′ UTR. The mechanism by which they act on translation has remained largely obscure. We examined the translation of mRNAs containing four partially mismatched miRNA-binding sites in the 3′ UTR in HeLa cells cotransfected with a cognate miRNA. The mRNAs were prepared by in vitro transcription and were engineered to employ different modes of translation initiation. We find that the 5′ cap structure and the 3′ poly(A) tail are each necessary but not sufficient for full miRNA-mediated repression of mRNA translation. Replacing the cap structure with an internal ribosome entry site from either the cricket paralysis virus or the encephalomyocarditis virus impairs miRNA-mediated repression. Collectively, these results demonstrate that miRNAs interfere with the initiation step of translation and implicate the cap-binding protein eukaryotic initiation factor 4E as a molecular target.
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Cites
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A pancreatic islet-specific microRNA regulates insulin secretion
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MicroRNAs Regulate Brain Morphogenesis in Zebrafish
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Molecular mechanisms of translational control
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Cited by
(2008) · doi:10.1038/nrg2290 · Cited by 5,199
Mammalian microRNAs predominantly act to decrease target mRNA levels
Guo, H., Ingolia, N. T., Weissman, J. S., & Bartel, D. P. (2010) · Nature · doi:10.1038/nature09267 · Cited by 3,886
Regulation of mRNA Translation and Stability by microRNAs
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Gene silencing by microRNAs: contributions of translational repression and mRNA decay
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Metadata
- Authors
- Humphreys, D. T., Westman, B. J., Martin, D. I. K., & Preiss, T.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2005-11-15
- Volume
- 102
- Issue
- 47
- Pages
- 16961-16966
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2096744132
- Cited by
- 655
- References
- 9
- FWCI
- 15.47
- Type
- journal article
- Language
- en
- Open access
- green
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- Humphreys, D. T., Westman, B. J., Martin, D. I. K., & Preiss, T. (2005). MicroRNAs control translation initiation by inhibiting eukaryotic initiation factor 4E/cap and poly(A) tail function. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.0506482102
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