The DOI matches its Crossref record; 33 of the 33 references it cites were found in a registry or an index.
A single lentiviral vector platform for microRNA-based conditional RNA interference and coordinated transgene expression
Abstract
RNAi is proving to be a powerful experimental tool for the functional annotation of mammalian genomes. The full potential of this technology will be realized through development of approaches permitting regulated manipulation of endogenous gene expression with coordinated reexpression of exogenous transgenes. We describe the development of a lentiviral vector platform, pSLIK (single lentivector for inducible knockdown), which permits tetracycline-regulated expression of microRNA-like short hairpin RNAs from a single viral infection of any naïve cell system. In mouse embryonic fibroblasts, the pSLIK platform was used to conditionally deplete the expression of the heterotrimeric G proteins Gα12 and Gα13 both singly and in combination, demonstrating the Gα13 dependence of serum response element-mediated transcription. In RAW264.7 macrophages, regulated knockdown of Gβ2 correlated with a reduced Ca 2+ response to C5a. Insertion of a GFP transgene upstream of the Gβ2 microRNA-like short hairpin RNA allowed concomitant reexpression of a heterologous mRNA during tetracycline-dependent target gene knockdown, significantly enhancing the experimental applicability of the pSLIK system.
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Tracing compartmentalized NADPH metabolism in the cytosol and mitochondria of mammalian cells
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Dimitrova, N., Zamudio, J. R., Jong, R. M., Soukup, D., Resnick, R., Sarma, K., Ward, A. J., Raj, A., Lee, J. T., Sharp, P. A., et al. (2014) · Molecular Cell · doi:10.1016/j.molcel.2014.04.025 · Cited by 445
Metadata
- Authors
- Shin, K.-J., Wall, E. A., Zavzavadjian, J. R., Santat, L. A., Liu, J., Hwang, J.-I., Rebres, R., Roach, T., Seaman, W., Simon, M. I., & Fraser, I. D. C.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2006-09-12
- Volume
- 103
- Issue
- 37
- Pages
- 13759-13764
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1965881928
- Cited by
- 332
- References
- 33
- FWCI
- 5.03
- Type
- journal article
- Language
- en
- Open access
- closed
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- Shin, K.-J., Wall, E. A., Zavzavadjian, J. R., Santat, L. A., Liu, J., Hwang, J.-I., Rebres, R., Roach, T., Seaman, W., Simon, M. I., & Fraser, I. D. C. (2006). A single lentiviral vector platform for microRNA-based conditional RNA interference and coordinated transgene expression. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.0606179103
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