Cited by 109Alzheimer's disease research and treatmentsDementia and Cognitive Impairment Researchbioluminescence and chemiluminescence research

Bioluminescence imaging of Aβ deposition in bigenic mouse models of Alzheimer's disease

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Watts, Joel C., Giles, Kurt, Grillo, Sunny K., Lemus, Azucena, DeArmond, Stephen J., Prusiner, Stanley B.·2011·Proceedings of the National Academy of Sciences

Abstract

Transgenic (Tg) mouse models of Alzheimer's disease have served as valuable tools for investigating pathogenic mechanisms related to Aβ accumulation. However, assessing disease status in these animals has required time-consuming behavioral assessments or postmortem neuropathological analysis. Here, we report a method for tracking the progression of Aβ accumulation in vivo using bioluminescence imaging (BLI) on two lines of Tg mice, which express luciferase (luc) under control of the Gfap promoter as well as mutant human amyloid precursor protein. Bigenic mice exhibited an age-dependent increase in BLI signals that correlated with the deposition of Aβ in the brain. Bioluminescence signals began to increase in 7-mo-old Tg(CRND8: Gfap -luc) mice and 14-mo-old Tg(APP23: Gfap -luc) mice. When Tg(APP23: Gfap -luc) mice were inoculated with brain homogenates from aged Tg(APP23) mice, BLI detected the accelerated disease onset and induced Aβ deposition at 11 mo of age. Because of its rapid, noninvasive, and quantitative format, BLI permits the objective repeated analysis of individual mice at multiple time points, which is likely to facilitate the testing of Aβ-directed therapeutics.

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Crossref · 10.1073/pnas.1019034108

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Authors
Watts, J. C., Giles, K., Grillo, S. K., Lemus, A., DeArmond, S. J., & Prusiner, S. B.
Affiliations
  • Institute for Neurodegenerative Diseases and
  • Institute for Neurodegenerative Diseases (US)
  • University of California, San Francisco (US)
Venue
Proceedings of the National Academy of Sciences
Published
2011-01-24
Volume
108
Issue
6
Pages
2528-2533
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
OpenAlex
W2133007412
Cited by
109
References
38
Citations by year
2026: 1 · 2025: 1 · 2023: 7 · 2022: 4 · 2021: 9 · 2020: 7 · 2019: 7 · 2018: 4 · 2017: 2 · 2016: 10 · 2015: 8 · 2014: 19 · 2013: 15 · 2012: 8
FWCI
10.69
Type
journal article
Language
en
Open access
Locations
  • Proceedings of the National Academy of Sciences (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Larry L. Hillblom Foundation
  • Lincy Foundation
  • Sherman Fairchild Foundation
  • National Institutes of Health
  • Canadian Institutes of Health Research
  • National Institute on Aging (AG010770, P01AG002132, P01AG021601, P01AG010770, AG002132, AG 021601, R37AG031220)
Topics
  • Health Sciences › Medicine › Physiology › Alzheimer's disease research and treatments
  • Health Sciences › Medicine › Psychiatry and Mental health › Dementia and Cognitive Impairment Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › bioluminescence and chemiluminescence research
Keywords
Alzheimer's disease, amyloid-beta deposition, bioluminescence imaging, transgenic mouse model, APP23 mice, non-invasive imaging
MeSH terms
Aging, Alzheimer Disease, Animals, Diagnostic Imaging, Disease Models, Animal, Glial Fibrillary Acidic Protein, Humans, Luciferases, Mice, Transgenic, Nerve Tissue Proteins, Promoter Regions, Genetic, Amyloid beta-Protein Precursor, Luminescence, Mice

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