The DOI matches its Crossref record; 48 of the 48 references it cites were found in a registry or an index.
Brassinosteroids regulate organ boundary formation in the shoot apical meristem of Arabidopsis
Open accessAbstract
Spatiotemporal control of the formation of organ primordia and organ boundaries from the stem cell niche in the shoot apical meristem (SAM) determines the patterning and architecture of plants, but the underlying signaling mechanisms remain poorly understood. Here we show that brassinosteroids (BRs) play a key role in organ boundary formation by repressing organ boundary identity genes. BR-hypersensitive mutants display organ-fusion phenotypes, whereas BR-insensitive mutants show enhanced organ boundaries. The BR-activated transcription factor BZR1 directly represses the CUP-SHAPED COTYLEDON ( CUC ) family of organ boundary identity genes. In WT plants, BZR1 accumulates at high levels in the nuclei of central meristem and organ primordia but at a low level in organ boundary cells to allow CUC gene expression. Activation of BR signaling represses CUC gene expression and causes organ fusion phenotypes. This study uncovers a role for BR in the spatiotemporal control of organ boundary formation and morphogenesis in the SAM.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The source backs part of the claim, not all of it — or backs it for a different population, condition or comparison.
In the citing paper
“The LOB1-mediated decrease of brassinosteroid activity causes a reduction of cell size and cell division rate in the boundary zone compared to neighboring zones (Bell et al., 2012; Gendron et al., 2012).”
From Shaping plant architecture (2015) · cites this paper as Gendron et al., 2012
Experimental — accuracy not yet measuredThe source backs part of the claim, not all of it — or backs it for a different population, condition or comparison.
In the citing paper
“In addition, LOF1 and CUC genes show complex interactions, regulating each other's expression (Lee et al., 2009;Gendron et al., 2012; Fig. 3).”
From Divide et impera: boundaries shape the plant body and initiate new meristems (2016) · cites this paper as Gendron et al., 2012
Experimental — accuracy not yet measuredThe cited source says what the claim attributes to it.
In the citing paper
“CUC genes are down-regulated by brassinosteroids.”
From Shaping plant architecture (2015) · cites this paper as Gendron et al., 2012
The cited source says what the claim attributes to it.
In the citing paper
“Thus, low brassinosteroid activity in the boundary zone not only reduces cell expansion and division as described above, but also allows the induction of CUC genes (Bell et al., 2012; Gendron et al., 2012).”
From Shaping plant architecture (2015) · cites this paper as Gendron et al., 2012
The cited source says what the claim attributes to it.
In the citing paper
“In wild type, BZR1 proteins accumulate to high levels in the central meristem and organ primordia, limiting the expression of a specific set of genes, including CUC genes, to the boundary (Gendron et al., 2012).”
From Divide et impera: boundaries shape the plant body and initiate new meristems (2016) · cites this paper as Gendron et al., 2012
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Genes involved in organ separation in Arabidopsis: an analysis of the cup-shaped cotyledon mutant.
Aida, M., Ishida, T., Fukaki, H., Fujisawa, H., & Tasaka, M. (1997) · The Plant Cell · doi:10.1105/tpc.9.6.841 · Cited by 1,486
BRASSINOSTEROIDS: Essential Regulators of Plant Growth and Development
Clouse, S. D., & Sasse, J. M. (1998) · Annual Review of Plant Physiology and Plant Molecular Biology · doi:10.1146/annurev.arplant.49.1.427 · Cited by 1,462
Heisler, M. G., Ohno, C., Das, P., Sieber, P., Reddy, G. V., Long, J. A., & Meyerowitz, E. M. (2005) · Current Biology · doi:10.1016/j.cub.2005.09.052 · Cited by 1,189
Wang, Z., Nakano, T., Gendron, J. M., He, J., Chen, M., Vafeados, D. K., Yang, Y., Fujioka, S., Yoshida, S., Asami, T., et al. (2002) · Developmental Cell · doi:10.1016/s1534-5807(02)00153-3 · Cited by 1,148
Self-citationSun, Y., Fan, X.-Y., Cao, D.-M., Tang, W., He, K., Zhu, J.-Y., He, J.-X., Bai, M., Zhu, S., Oh, E., et al. (2010) · Developmental Cell · doi:10.1016/j.devcel.2010.10.010 · Cited by 1,002
Self-citationSzekeres, M., Németh, K., Koncz–Kálmán, Z., Mathur, J., Kauschmann, A., Altmann, T., Rédei, G. P., Nagy, F., Schell, J., & Koncz, C. (1996) · Cell · doi:10.1016/s0092-8674(00)81094-6 · Cited by 998
Interaction between BZR1 and PIF4 integrates brassinosteroid and environmental responses
Oh, E., Zhu, J.-Y., & Wang, Z. (2012) · Nature Cell Biology · doi:10.1038/ncb2545 · Cited by 886
Self-citationHe, J., Gendron, J. M., Sun, Y., Gampala, S. S. L., Gendron, N., Sun, C. Q., & Wang, Z. (2005) · Science · doi:10.1126/science.1107580 · Cited by 868
Self-citationA Role for Brassinosteroids in Light-Dependent Development of Arabidopsis
Li, J., Nagpal, P., Vitart, V., McMorris, T. C., & Chory, J. (1996) · Science · doi:10.1126/science.272.5260.398 · Cited by 856
Yamamuro, C., Ihara, Y., Wu, X., Noguchi, T., Fujioka, S., Takatsuto, S., Ashikari, M., Kitano, H., & Matsuoka, M. (2000) · The Plant Cell · doi:10.1105/tpc.12.9.1591 · Cited by 758
He, J.-X., Gendron, J. M., Yang, Y., Li, J., & Wang, Z. (2002) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.152342599 · Cited by 719
Self-citationProgesterone induces adult mammary stem cell expansion
Joshi, P. A., Jackson, H. W., Beristain, A. G., Grappa, M. A. D., Mote, P. A., Clarke, C. L., Stingl, J., Waterhouse, P. D., & Khokha, R. (2010) · Nature · doi:10.1038/nature09091 · Cited by 698
Control of mammary stem cell function by steroid hormone signalling
Asselin-Labat, M.-L., Vaillant, F., Sheridan, J. M., Pal, B., Wu, D., Simpson, E. R., Yasuda, H., Smyth, G. K., Martin, T. J., Lindeman, G. J., et al. (2010) · Nature · doi:10.1038/nature09027 · Cited by 693 · cited 1×
“Conversely, the recent findings of positive effects of ovarian steroid hormones on stem cell proliferation in mammals (46, 47) suggest that BR promotion of boundary cell growth may reflect an ancient function of steroid hormones in promoting stem cell proliferation.”
BackgroundBrassinosteroid, gibberellin and phytochrome impinge on a common transcription module in Arabidopsis
Bai, M., Shang, J., Oh, E., Fan, M., Bai, Y., Zentella, R., Sun, T., & Wang, Z. (2012) · Nature Cell Biology · doi:10.1038/ncb2546 · Cited by 684
Self-citationBrassinosteroid Signal Transduction from Receptor Kinases to Transcription Factors
Kim, T., & Wang, Z. (2010) · Annual Review of Plant Biology · doi:10.1146/annurev.arplant.043008.092057 · Cited by 650
Self-citationThe Balance between the MIR164A and CUC2 Genes Controls Leaf Margin Serration in Arabidopsis
Nikovics, K., Blein, T., Peaucelle, A., Ishida, T., Morin, H., Aida, M., & Laufs, P. (2006) · The Plant Cell · doi:10.1105/tpc.106.045617 · Cited by 624
Aida, M., Ishida, T., & Tasaka, M. (1999) · Development · doi:10.1242/dev.126.8.1563 · Cited by 557
The Lateral Organ Boundaries Gene Defines a Novel, Plant-Specific Gene Family
Shuai, B., Reynaga-Peña, C. G., & Springer, P. S. (2002) · Plant Physiology · doi:10.1104/pp.010926 · Cited by 555
Self-citationBrassinosteroid regulates stomatal development by GSK3-mediated inhibition of a MAPK pathway
Kim, T., Michniewicz, M., Bergmann, D. C., & Wang, Z. (2012) · Nature · doi:10.1038/nature10794 · Cited by 530
Self-citationAn Essential Role for 14-3-3 Proteins in Brassinosteroid Signal Transduction in Arabidopsis
Gampala, S. S. L., Kim, T., He, J., Tang, W., Deng, Z., Bai, M.-Y., Guan, S., Lalonde, S., Sun, Y., Gendron, J. M., et al. (2007) · Developmental Cell · doi:10.1016/j.devcel.2007.06.009 · Cited by 524
Self-citationBrassinosteroids control meristem size by promoting cell cycle progression in Arabidopsis roots
González‐García, M. P., Vilarrasa‐Blasi, J., Zhiponova, M., Divol, F., Mora‐García, S., Russinova, E., & Caño‐Delgado, A. I. (2011) · Development · doi:10.1242/dev.057331 · Cited by 522
PP2A activates brassinosteroid-responsive gene expression and plant growth by dephosphorylating BZR1
Tang, W., Yuan, M., Wang, R., Yang, Y., Wang, C., Osés-Prieto, J. A., Kim, T., Zhou, H., Deng, Z., Gampala, S. S. L., et al. (2011) · Nature Cell Biology · doi:10.1038/ncb2151 · Cited by 499
Self-citationZhang, L., Bai, M., Wu, J., Zhu, J., Wang, H., Zhang, Z., Wang, W., Sun, Y., Zhao, J., Sun, X., et al. (2009) · The Plant Cell · doi:10.1105/tpc.109.070441 · Cited by 488
Self-citationStructural insight into brassinosteroid perception by BRI1
She, J., Han, Z., Kim, T., Wang, J., Cheng, W., Chang, J., Shi, S., Wang, J., Yang, M., Wang, Z., et al. (2011) · Nature · doi:10.1038/nature10178 · Cited by 421
Self-citationStructural basis of steroid hormone perception by the receptor kinase BRI1
Hothorn, M., Belkhadir, Y., Dreux, M., Dabi, T., Noel, J. P., Wilson, I. A., & Chory, J. (2011) · Nature · doi:10.1038/nature10153 · Cited by 419
MOLECULAR MECHANISMS OF STEROID HORMONE SIGNALING IN PLANTS
Vert, G., Nemhauser, J. L., Geldner, N., Hong, F., & Chory, J. (2005) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev.cellbio.21.090704.151241 · Cited by 404
Hibara, K.-I., Karim, M. R., Takada, S., Taoka, K.-I., Furutani, M., Aida, M., & Tasaka, M. (2006) · The Plant Cell · doi:10.1105/tpc.106.045716 · Cited by 387
Brassinosteroid perception in the epidermis controls root meristem size
Hacham, Y., Holland, N., Butterfield, C. N., Úbeda-Tomás, S., Bennett, M. J., Chory, J., & Savaldi‐Goldstein, S. (2011) · Development · doi:10.1242/dev.061804 · Cited by 375
The NAC-domain transcription factor GOBLET specifies leaflet boundaries in compound tomato leaves
Berger, Y., Harpaz-Saad, S., Brand, A., Melnik, H., Sirding, N., Alvarez, J. P., Zinder, M., Samach, A., Eshed, Y., & Ori, N. (2009) · Development · doi:10.1242/dev.031625 · Cited by 305
The march of the PINs: developmental plasticity by dynamic polar targeting in plant cells
Grunewald, W., & Friml, J. (2010) · The EMBO Journal · doi:10.1038/emboj.2010.181 · Cited by 285
Luo, X.-M., Lin, W.-H., Zhu, S., Zhu, J.-Y., Sun, Y., Fan, X.-Y., Cheng, M., Hao, Y., Oh, E., Tian, M., et al. (2010) · Developmental Cell · doi:10.1016/j.devcel.2010.10.023 · Cited by 285
Self-citationFurutani, M., Vernoux, T., Traas, J., Kato, T., Tasaka, M., & Aida, M. (2004) · Development · doi:10.1242/dev.01388 · Cited by 271
Roles ofPIN-FORMED1andMONOPTEROSin pattern formation of the apical region of theArabidopsisembryo
Aida, M., Vernoux, T., Furutani, M., Traas, J. A., & Tasaka, M. (2002) · Development · doi:10.1242/dev.129.17.3965 · Cited by 250
The Role of OsBRI1 and Its Homologous Genes, OsBRL1 and OsBRL3 , in Rice
Nakamura, A., Fujioka, S., Sunohara, H., Kamiya, N., Hong, Z., Inukai, Y., Miura, K., Takatsuto, S., Yoshida, S., Ueguchi‐Tanaka, M., et al. (2006) · PLANT PHYSIOLOGY · doi:10.1104/pp.105.072330 · Cited by 214
Bell, E. M., Lin, W.-C., Husbands, A. Y., Yu, L., Jaganatha, V., Jablonska, B., Mangeon, A., Neff, M. M., Girke, T., & Springer, P. S. (2012) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1210789109 · Cited by 194
Self-citationSymons, G. M., & Reid, J. B. (2004) · PLANT PHYSIOLOGY · doi:10.1104/pp.104.043034 · Cited by 191
Ibañes, M., Fàbregas, N., Chory, J., & Caño‐Delgado, A. I. (2009) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0906416106 · Cited by 181
Auxin stimulates DWARF4 expression and brassinosteroid biosynthesis in Arabidopsis
Chung, Y., Maharjan, P. M., Lee, O., Fujioka, S., Jang, S., Kim, B., Takatsuto, S., Tsujimoto, M., Kim, H., Cho, S., et al. (2011) · The Plant Journal · doi:10.1111/j.1365-313x.2011.04513.x · Cited by 168
Lee, D.-K., Geisler, M., & Springer, P. S. (2009) · Development · doi:10.1242/dev.031971 · Cited by 164
Self-citationGenetic control of shoot organ boundaries
Aida, M., & Tasaka, M. (2005) · Current Opinion in Plant Biology · doi:10.1016/j.pbi.2005.11.011 · Cited by 159
Multiple mechanisms modulate brassinosteroid signaling
Gendron, J. M., & Wang, Z. (2007) · Current Opinion in Plant Biology · doi:10.1016/j.pbi.2007.08.015 · Cited by 133
Self-citationMorphogenesis and Patterning at the Organ Boundaries in the Higher Plant Shoot Apex
Aida, M., & Tasaka, M. (2006) · Plant Molecular Biology · doi:10.1007/s11103-005-2760-7 · Cited by 117
Kwon, C. S., Hibara, K., Pfluger, J., Bezhani, S., Metha, H., Aida, M., Tasaka, M., & Wagner, D. (2006) · Development · doi:10.1242/dev.02508 · Cited by 116
The dynamic plant stem cell niches
Sablowski, R. W. M. (2007) · Current Opinion in Plant Biology · doi:10.1016/j.pbi.2007.07.001 · Cited by 105
Holton, N. J., Caño‐Delgado, A. I., Harrison, K., Montoya, T., Chory, J., & Bishop, G. J. (2007) · The Plant Cell · doi:10.1105/tpc.106.047795 · Cited by 98
The meristem-to-organ boundary: more than an extremity of anything
Rast-Somssich, M. I., & Simon, R. G. (2008) · Current Opinion in Genetics & Development · doi:10.1016/j.gde.2008.05.005 · Cited by 90
Plant and animal stem cells: conceptually similar, molecularly distinct?
Sablowski, R. W. M. (2004) · Trends in Cell Biology · doi:10.1016/j.tcb.2004.09.011 · Cited by 86
Cheon, J., Park, S., Schulz, B., & Choe, S. (2010) · BMC Plant Biology · doi:10.1186/1471-2229-10-270 · Cited by 70
Cited by
Sorted by citations
Lateral organ boundaries 1 is a disease susceptibility gene for citrus bacterial canker disease
Hu, Y., Zhang, J., Jia, H., Sosso, D., Li, T., Frommer, W. B., Yang, B., White, F. F., Wang, N., & Jones, J. B. (2014) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1313271111 · Cited by 356
Chaiwanon, J., & Wang, Z. (2015) · Current Biology · doi:10.1016/j.cub.2015.02.046 · Cited by 295
Self-citationBrassinosteroid Regulates Seed Size and Shape in Arabidopsis
Jiang, W.-B., Huang, H.-Y., Hu, Y.-W., Zhu, S.-W., Wang, Z., & Lin, W.-H. (2013) · PLANT PHYSIOLOGY · doi:10.1104/pp.113.217703 · Cited by 270
Self-citationThe molecular circuitry of brassinosteroid signaling
Belkhadir, Y., & Jaillais, Y. (2015) · New Phytologist · doi:10.1111/nph.13269 · Cited by 253
Teichmann, T., & Muhr, M. (2015) · Frontiers in Plant Science · doi:10.3389/fpls.2015.00233 · Cited by 228
“The LOB1-mediated decrease of brassinosteroid activity causes a reduction of cell size and cell division rate in the boundary zone compared to neighboring zones (Bell et al., 2012; Gendron et al., 2012).”
Background
Metadata
- Authors
- Gendron, J. M., Liu, J.-S., Fan, M., Bai, M.-Y., Wenkel, S., Springer, P. S., Barton, M. K., & Wang, Z.-Y.
- Affiliations
- Carnegie Institution for Science (US)
- Department of Plant Biology, Carnegie Institution for Science, Stanford, CA 94305; and
- University of California, Riverside (US)
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 2012-12-04
- Volume
- 109
- Issue
- 51
- Pages
- 21152-21157
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC3529081
- OpenAlex
- W2141148584
- Cited by
- 205
- References
- 48
- Citations by year
- 2026: 14 · 2025: 17 · 2024: 14 · 2023: 8 · 2022: 11 · 2021: 15 · 2020: 15 · 2019: 14 · 2018: 11 · 2017: 19 · 2016: 10 · 2015: 26 · 2014: 12 · 2013: 17 · 2012: 2
- FWCI
- 20.84
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute of General Medical Sciences (R01GM066258)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Parasitism and Resistance
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant tissue culture and regeneration
- Keywords
- brassinosteroids, shoot apical meristem, BZR1, organ primordia, transcriptional repression, plant morphogenesis
- MeSH terms
- Cell Nucleus, Microscopy, Electron, Scanning, Mutation, Phenotype, Signal Transduction, Genes, Plant, Arabidopsis, Gene Expression Regulation, Plant, Meristem, Plant Shoots, Microscopy, Confocal, Arabidopsis Proteins, Chromatin Immunoprecipitation, Brassinosteroids
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
