Extracellular matrix requirements for gastrointestinal organoid cultures
Open accessAbstract
Organoids are a new class of biological model systems that have garnered significant interest in the life sciences. When provided with the proper 3D matrix and biochemical factors, stem cells can self-organize and form tissue-specific organoids. Thus far, there has been a substantial effort to identify soluble niche components essential for organoid culture; however, the role of the solid extracellular matrix (ECM) as an essential element of the niche is still largely lacking. In this review, we discuss the importance of the ECM in intestinal, hepatic, and pancreatic organoid culture and how biomaterial-based approaches can be used to probe different ECM properties required for more physiologically and translationally relevant organoid models.
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Many other organoid models, including brain, intestinal, mammary gland, and retinal organoids, employ 3D ECM-based hydrogels to induce complex tissue architecture that cannot be achieved in ECM-free floating culture.
“While many other organoid models, including brain[13], intestinal [14], mammary gland [15], and retinal organoids [16], employ 3D ECM-based hydrogels to induce complex tissue architecture that cannot be achieved in ECM-free floating culture, the same has not been explored for skin yet.”
From Efficient Generation of Skin Organoids from Pluripotent Cells via Defined Extracellular Matrix Cues and Morphogen Gradients in a Spindle‐Shaped Microfluidic Device (2024) · cites this paper as [14]
Citation [14] is attached to intestinal organoids. Section 3.2 (page unavailable) says intestinal organoid cultures rely on Matrigel and describes hydrogels that support intestinal morphogenesis. It does not establish the claimed comparison that complex architecture cannot be achieved in ECM-free floating culture; the other organoid types have separate citations. (LLM, conf=0.84)
Experimental — accuracy not yet measuredMaximizing mass transfer and minimizing shear stress in the perfusive soluble microenvironment causes cells grown in organ-on-a-chip set-ups to upregulate their functions, getting closer to native tissue.
“It has been shown that by maximizing the mass transfer and minimizing the shear stress in the perfusive soluble microenvironment, cells grown in organ-on-a-chip set-ups upregulate their functions, getting a step closer to a native tissue94,244,245.”
From Organoids (2022) · cites this paper as [244]
The Conclusion and future perspectives discusses how ECM mechanics and synthetic matrices may improve organoid maturation, not how perfusion affects cells in organ-on-a-chip setups. Searches for organ-on-a-chip, mass transfer, and shear stress found no passage linking optimized flow and reduced shear stress to upregulated cell function. (LLM, conf=0.76)
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- Authors
- Rezakhani, S., Gjorevski, N., & Lutolf, M. P.
- Affiliations
- École Polytechnique Fédérale de Lausanne (CH)
- Venue
- Biomaterials
- Publisher
- Elsevier BV
- Published
- 2021-09
- Volume
- 276
- Pages
- 121020
- ISSN
- 0142-9612 (ISSN-L)
- OpenAlex
- W3177835004
- Cited by
- 100
- References
- 123
- Citations by year
- 2026: 18 · 2025: 23 · 2024: 17 · 2023: 19 · 2022: 22 · 2021: 1
- FWCI
- 1.24
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Biomaterials (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- Infoscience (Ecole Polytechnique Fédérale de Lausanne) (submittedVersion, cc-by-nc-nd)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Keywords
- extracellular matrix, organoids, intestinal organoids, hepatic organoids, pancreatic organoids, stem cell niche, organoid culture
- MeSH terms
- Biocompatible Materials, Extracellular Matrix, Intestines, Organoids, Stem Cells
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
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