Highly compacted biodegradable DNA nanoparticles capable of overcoming the mucus barrier for inhaled lung gene therapy
Open accessAbstract
Significance: Therapeutically relevant lung gene therapy is yet to be achieved. We introduce a highly translatable gene delivery platform for inhaled gene therapy based on state-of-the-art biodegradable polymers, poly(β-amino esters). The newly designed system is capable of overcoming challenging biological barriers, thereby providing robust transgene expression throughout the entire luminal surface of mouse lungs. Moreover, it provides markedly greater overall transgene expression in vivo compared with gold standard platforms, including a clinically tested system. The clinical relevance is further underscored by the excellent safety profile as well as long-term and consistent transgene expression achieved following a single and repeated administrations, respectively.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Biodegradable DNA NPs have shown potential for inhaled lung gene therapy, capable of overcoming mucus barrier of lung airways.
“In recent times, biodegradable DNA NPs have shown potential for inhaled lung gene therapy, which is capable of overcoming the mucus barrier of lung airways.183”
From Targeted therapy in chronic diseases using nanomaterial-based drug delivery vehicles (2019) · cites this paper as [183]
Cites
Nonviral vectors for gene delivery
Mintzer, M. A., & Simanek, E. E. (2009) · Chemical Reviews · doi:10.1021/cr800409e · Cited by 2,366 · see citing works
Mucus-penetrating nanoparticles for drug and gene delivery to mucosal tissues
Lai, S. K., Wang, Y.-Y., & Hanes, J. S. (2008) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2008.11.002 · Cited by 1,827 · see citing works
Self-citationCone, R. A. (2008) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2008.09.008 · Cited by 1,459 · see citing works
Degradable poly (β-amino esters): synthesis, characterization, and self-assembly with plasmid DNA
Lynn, D. M., & Langer, R. (2000) · Journal of the American Chemical Society · doi:10.1021/ja0015388 · Cited by 924 · see citing works
Mishra, S., Webster, P. C., & Davis, M. E. (2004) · European Journal of Cell Biology · doi:10.1078/0171-9335-00363 · Cited by 733 · see citing works
Cell number and cell characteristics of the normal human lung.
Crapo, J. D., Barry, B. E., Gehr, P., Bachofen, M., & Weibel, E. R. (1982) · PubMed · doi:10.1164/arrd.1982.125.6.740 · Cited by 730 · see citing works
Immune responses to gene therapy vectors: influence on vector function and effector mechanisms
Bessis, N., Garcia-Cozar, F. J., & Boissier, M. (2004) · Gene Therapy · doi:10.1038/sj.gt.3302364 · Cited by 636 · see citing works
New Drugs for Rheumatoid Arthritis
Olsen, N. J., & Stein, C. (2004) · New England Journal of Medicine · doi:10.1056/nejmra032906 · Cited by 632 · see citing works
A combinatorial polymer library approach yields insight into nonviral gene delivery
Green, J. J., Langer, R., & Anderson, D. G. (2008) · Accounts of Chemical Research · doi:10.1021/ar7002336 · Cited by 579 · see citing works
Nanoparticle diffusion in respiratory mucus from humans without lung disease
Schuster, B. S., Suk, J. S., Woodworth, G. F., & Hanes, J. S. (2013) · Biomaterials · doi:10.1016/j.biomaterials.2013.01.064 · Cited by 468 · see citing works
Self-citationAnalysis of in vivo dynamics of influenza virus infection in mice using a GFP reporter virus
Manicassamy, B., Manicassamy, S., Belicha‐Villanueva, A., Pisanelli, G., Pulendran, B., & García‐Sastre, A. (2010) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0914994107 · Cited by 424 · see citing works
Airway Epithelial Cells: Current Concepts and Challenges
Crystal, R. G., Randell, S. H., Engelhardt, J. F., Voynow, J. A., & Sunday, M. E. (2008) · Proceedings of the American Thoracic Society · doi:10.1513/pats.200805-041hr · Cited by 401 · see citing works
Moss, R. B., Rodman, D. M., Spencer, L. T., Aitken, M. L., Zeitlin, P. L., Waltz, D. A., Milla, C. E., Brody, A. S., Clancy, J. P., Ramsey, B. W., et al. (2004) · CHEST Journal · doi:10.1378/chest.125.2.509 · Cited by 389 · see citing works
Structure/property studies of polymeric gene delivery using a library of poly(β-amino esters)
Anderson, D. G., Akinc, A., Hossain, N., & Langer, R. (2004) · Molecular Therapy · doi:10.1016/j.ymthe.2004.11.015 · Cited by 361 · see citing works
Suk, J. S., Lai, S. K., Wang, Y.-Y., Ensign, L. M., Zeitlin, P. L., Boyle, M., & Hanes, J. S. (2009) · Biomaterials · doi:10.1016/j.biomaterials.2008.12.076 · Cited by 355 · see citing works
Self-citationIn Vivo Gene Delivery by Nonviral Vectors: Overcoming Hurdles?
Zhang, Y., Satterlee, A., & Huang, L. (2012) · Molecular Therapy · doi:10.1038/mt.2012.79 · Cited by 353 · see citing works
Estimating the effective density of engineered nanomaterials for in vitro dosimetry
DeLoid, G., Cohen, J. M., Darrah, T., Derk, R., Rojanasakul, L., Pyrgiotakis, G., Wohlleben, W., & Demokritou, P. (2014) · Nature Communications · doi:10.1038/ncomms4514 · Cited by 327 · see citing works
Ciliated epithelial cell lifespan in the mouse trachea and lung
Rawlins, E. L., & Hogan, B. L. M. (2008) · American Journal of Physiology-Lung Cellular and Molecular Physiology · doi:10.1152/ajplung.90209.2008 · Cited by 304 · see citing works
Sheridan, C. (2011) · Nature Biotechnology · doi:10.1038/nbt.1769 · Cited by 302 · see citing works
Nanoparticle formulations in pulmonary drug delivery
Bailey, M. M., & Berkland, C. J. (2009) · Medicinal Research Reviews · doi:10.1002/med.20140 · Cited by 302 · see citing works
Konstan, M. W., Davis, P. B., Wagener, J. S., Hilliard, K. A., Stern, R. C., Milgram, L. J. H., Kowalczyk, T. H., Hyatt, S. L., Fink, T. L., Gedeon, C. R., et al. (2004) · Human Gene Therapy · doi:10.1089/hum.2004.15.1255 · Cited by 298 · see citing works
Mucus Penetrating Nanoparticles: Biophysical Tool and Method of Drug and Gene Delivery
Ensign, L. M., Schneider, C. S., Suk, J. S., Cone, R. A., & Hanes, J. S. (2012) · Advanced Materials · doi:10.1002/adma.201201800 · Cited by 293 · see citing works
Self-citationSynthesis of Poly(β-amino ester)s Optimized for Highly Effective Gene Delivery
Akinc, A., Anderson, D. G., Lynn, D. M., & Langer, R. (2003) · Bioconjugate Chemistry · doi:10.1021/bc034067y · Cited by 285 · see citing works
Moss, R. B., Milla, C. E., Colombo, J. L., Accurso, F. J., Zeitlin, P. L., Clancy, J. P., Spencer, L. T., Pilewski, J. M., Waltz, D. A., Dorkin, H. L., et al. (2007) · Human Gene Therapy · doi:10.1089/hum.2007.022 · Cited by 276 · see citing works
Real-time multiple-particle tracking: applications to drug and gene delivery
Suh, J., Dawson, M., & Hanes, J. S. (2004) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2004.06.001 · Cited by 274 · see citing works
Self-citationSanders, N. N., Smedt, S. C. D., Rompaey, E. V., Simoens, P., Baets, F. D., & Demeester, J. (2000) · American Journal of Respiratory and Critical Care Medicine · doi:10.1164/ajrccm.162.5.9909009 · Cited by 247 · see citing works
A pure population of lung alveolar epithelial type II cells derived from human embryonic stem cells
Wang, D., Haviland, D. L., Burns, A. R., Zsigmond, E. M., & Wetsel, R. A. (2007) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0700052104 · Cited by 246 · see citing works
Harvey, B., Leopold, P. L., Hackett, N. R., Grasso, T. M., Williams, P. M., Tucker, A. L., Kaner, R. J., Ferris, B., Gonda, I., Sweeney, T. D., et al. (1999) · Journal of Clinical Investigation · doi:10.1172/jci7935 · Cited by 244 · see citing works
Extracellular barriers in respiratory gene therapy
Sanders, N. N., Rudolph, C., Braeckmans, K., Smedt, S. C. D., & Demeester, J. (2008) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2008.09.011 · Cited by 229 · see citing works
Guerrero‐Cazares, H., Tzeng, S. Y., Young, N. P., Abutaleb, A., Quiñones‐Hinojosa, A., & Green, J. J. (2014) · ACS Nano · doi:10.1021/nn501197v · Cited by 221 · see citing works
Ogris, M., Steinlein, P., Carotta, S., Brunner, S., & Wagner, E. (2001) · AAPS PharmSci · doi:10.1208/ps030321 · Cited by 208 · see citing works
Cystic Fibrosis and Other Respiratory Diseases of Impaired Mucus Clearance
Livraghi-Butrico, A., & Randell, S. H. (2007) · Toxicologic Pathology · doi:10.1080/01926230601060025 · Cited by 196 · see citing works
Lung gene therapy with highly compacted DNA nanoparticles that overcome the mucus barrier
Suk, J. S., Kim, A. J., Trehan, K., Schneider, C. S., Cebotaru, L., Woodward, O. M., Boylan, N. J., Boyle, M., Lai, S. K., Guggino, W. B., et al. (2014) · Journal of Controlled Release · doi:10.1016/j.jconrel.2014.01.007 · Cited by 193 · see citing works
Self-citationGene silencing as a threat to the success of gene therapy
Bestor, T. H. (2000) · Journal of Clinical Investigation · doi:10.1172/jci9459 · Cited by 182 · see citing works
Sunshine, J. C., Peng, D. Y., & Green, J. J. (2012) · Molecular Pharmaceutics · doi:10.1021/mp3004176 · Cited by 173 · see citing works
Biodegradable Polymeric Vectors for Gene Delivery to Human Endothelial Cells
Green, J. J., Shi, J., Chiu, E., Leshchiner, E. S., Langer, R., & Anderson, D. G. (2006) · Bioconjugate Chemistry · doi:10.1021/bc0600968 · Cited by 166 · see citing works
Recent Developments in Nucleic Acid Delivery with Polyethylenimines
Neuberg, P., & Kichler, A. (2014) · Advances in genetics · doi:10.1016/b978-0-12-800148-6.00009-2 · Cited by 163 · see citing works
Rapid Optimization of Gene Delivery by Parallel End-modification of Poly(β-amino ester)s
Zugates, G. T., Peng, W., Zumbuehl, A., Jhunjhunwala, S., Huang, Y.-H., Langer, R., Sawicki, J. A., & Anderson, D. G. (2007) · Molecular Therapy · doi:10.1038/sj.mt.6300132 · Cited by 134 · see citing works
Mucus altering agents as adjuncts for nonviral gene transfer to airway epithelium
Ferrari, S., Kitson, C., Farley, R., Steel, R. M., Marriott, C., Parkins, D., Scarpa, M., Wainwright, B. J., Evans, M., Colledge, W. H., et al. (2001) · Gene Therapy · doi:10.1038/sj.gt.3301525 · Cited by 120 · see citing works
Pseudotyped human lentiviral vector-mediated gene transfer to airway epithelia in vivo
Johnson, L. G., Olsen, J. C., Naldini, L., & Boucher, R. C. (2000) · Gene Therapy · doi:10.1038/sj.gt.3301138 · Cited by 104 · see citing works
McLachlan, G., Davidson, H. M., Holder, E. L., Davies, L. A., Pringle, I. A., Jones, S., Baker, A., Tennant, P. A., Gordon, C., Vrettou, C., et al. (2011) · Gene Therapy · doi:10.1038/gt.2011.55 · Cited by 104 · see citing works
Comparative in vivo study of poly(ethylene imine)/siRNA complexes for pulmonary delivery in mice
Beyerle, A., Braun, A., Merkel, O. M., Koch, F., Kissel, T., & Stoeger, T. (2011) · Journal of Controlled Release · doi:10.1016/j.jconrel.2010.12.017 · Cited by 97 · see citing works
Suk, J. S., Boylan, N. J., Trehan, K., Tang, B. C., Schneider, C. S., Lin, J.-M. G., Boyle, M. P., Zeitlin, P. L., Lai, S. K., Cooper, M. J., et al. (2011) · Molecular Therapy · doi:10.1038/mt.2011.160 · Cited by 94 · see citing works
Self-citationGene Delivery Properties of End-Modified Poly(β-amino ester)s
Zugates, G. T., Tedford, N. C., Zumbuehl, A., Jhunjhunwala, S., Kang, C. S., Griffith, L. G., Lauffenburger, D. A., Langer, R., & Anderson, D. G. (2007) · Bioconjugate Chemistry · doi:10.1021/bc7002082 · Cited by 92 · see citing works
Wilson, A. A., Murphy, G. J., Hamakawa, H., Kwok, L. W., Srinivasan, S., Hovav, A., Mulligan, R. C., Amar, S., Suki, B., & Kotton, D. N. (2009) · Journal of Clinical Investigation · doi:10.1172/jci36666 · Cited by 91 · see citing works
Overcoming the Cystic Fibrosis Sputum Barrier to Leading Adeno-associated Virus Gene Therapy Vectors
Schuster, B. S., Kim, A. J., Kays, J. C., Kanzawa, M. M., Guggino, W. B., Boyle, M., Rowe, S. M., Muzyczka, N., Suk, J. S., & Hanes, J. S. (2014) · Molecular Therapy · doi:10.1038/mt.2014.89 · Cited by 90 · see citing works
Self-citationBoylan, N. J., Suk, J. S., Lai, S. K., Jelinek, R., Boyle, M., Cooper, M. J., & Hanes, J. S. (2011) · Journal of Controlled Release · doi:10.1016/j.jconrel.2011.08.031 · Cited by 89 · see citing works
Self-citationKeeney, M., Ong, S., Padilla-Lopez, A., Yao, Z., Goodman, S. B., Wu, J. C., & Yang, F. (2013) · ACS Nano · doi:10.1021/nn402657d · Cited by 87 · see citing works
Highly PEGylated DNA Nanoparticles Provide Uniform and Widespread Gene Transfer in the Brain
Mastorakos, P., Zhang, C., Berry, S., Oh, Y., Lee, S., Eberhart, C. G., Woodworth, G. F., Suk, J. S., & Hanes, J. (2015) · Advanced Healthcare Materials · doi:10.1002/adhm.201400800 · Cited by 80 · see citing works
Self-citationStructural Alterations of Gene Complexes by Cystic Fibrosis Sputum
Sanders, N. N., Rompaey, E. V., Smedt, S. C. D., & Demeester, J. (2001) · American Journal of Respiratory and Critical Care Medicine · doi:10.1164/ajrccm.164.3.2011041 · Cited by 79 · see citing works
Common Gene Therapy Viral Vectors Do Not Efficiently Penetrate Sputum from Cystic Fibrosis Patients
Hida, K., Lai, S. K., Suk, J. S., Won, S. Y., Boyle, M., & Hanes, J. S. (2011) · PLoS ONE · doi:10.1371/journal.pone.0019919 · Cited by 77 · see citing works
Self-citationKim, A. J., Boylan, N. J., Suk, J. S., Hwangbo, M., Yu, T., Schuster, B. S., Cebotaru, L., Lesniak, W. G., Oh, J. S., Adstamongkonkul, P., et al. (2013) · Angewandte Chemie International Edition · doi:10.1002/anie.201208556 · Cited by 64 · see citing works
Self-citationKuper, C. F., Gröllers‐Mulderij, M., Maarschalkerweerd, T. V. P., Meulendijks, N. M. M., Reus, A. A., van Acker, F., den Beuken, E. Z.-V., Wouters, M., Bijlsma, S., & Kooter, I. M. (2014) · Toxicology in Vitro · doi:10.1016/j.tiv.2014.10.017 · Cited by 61 · see citing works
Mucociliary Transport and Cough in Humans
Foster, W. M. (2002) · Pulmonary Pharmacology & Therapeutics · doi:10.1006/pupt.2002.0351 · Cited by 59 · see citing works
Gene Therapy for Pulmonary Diseases
West, J. D., & Rodman, D. M. (2001) · CHEST Journal · doi:10.1378/chest.119.2.613 · Cited by 57 · see citing works
Gene Therapy for Pulmonary Diseases
Kolb, M. R. J., Martin, G. E. M., Medina, M. F. C., Ask, K., & Gauldie, J. (2006) · CHEST Journal · doi:10.1378/chest.130.3.879 · Cited by 54 · see citing works
β–Amino Ester Polymers Facilitate in Vivo DNA Transfection and Adjuvant Plasmid DNA Immunization
Greenland, J. R., Liu, H., Berry, D., Anderson, D. G., Kim, W., Irvine, D. J., Langer, R., & Letvin, N. L. (2005) · Molecular Therapy · doi:10.1016/j.ymthe.2005.01.021 · Cited by 53 · see citing works
Poly(β-amino esters): Procedures for Synthesis and Gene Delivery
Green, J. J., Zugates, G. T., Langer, R., & Anderson, D. G. (2008) · Methods in molecular biology · doi:10.1007/978-1-59745-429-2_4 · Cited by 49 · see citing works
Nanoparticle-based therapy for respiratory diseases
SILVA, A. L. D., SANTOS, R. S., XISTO, D. G., ALONSO, S. D. V., MORALES, M. M., & ROCCO, P. R. M. (2013) · Anais da Academia Brasileira de Ciências · doi:10.1590/s0001-37652013005000018 · Cited by 42 · see citing works
Self-citationProgress and Prospects: prospects of repeated pulmonary administration of viral vectors
Sinn, P. L., Burnight, E. R., & McCray, P. B. (2009) · Gene Therapy · doi:10.1038/gt.2009.87 · Cited by 35 · see citing works
Sendai virus for gene therapy and vaccination.
Uta Griesenbach, D., Inoue, M., Hasegawa, M., & Alton, E. W. F. W. (2005) · PubMed · Cited by 33 · see citing works
Uta Griesenbach, D., Vicente, C. C., Roberts, M. J., Meng, C., Soussi, S., Xenariou, S., Tennant, P. A., Baker, A., Baker, E., Gordon, C., et al. (2011) · Biomaterials · doi:10.1016/j.biomaterials.2010.12.001 · Cited by 30 · see citing works
Real-Time Multiple Particle Tracking of Gene Nanocarriers in Complex Biological Environments
Lai, S. K., & Hanes, J. S. (2008) · Humana Press eBooks · doi:10.1007/978-1-60327-248-3_6 · Cited by 26 · see citing works
Self-citationKang, S., Lu, K., Leelawattanachai, J., Hu, X., Park, S., Park, T., Min, I. M., & Jin, M. M. (2013) · Gene Therapy · doi:10.1038/gt.2013.29 · Cited by 14 · see citing works
Flow Cytometry-Based Cell Type-Specific Assessment of Target Regulation by Pulmonary siRNA Delivery
Merkel, O. M., Marsh, L. M., Garn, H., & Kissel, T. (2012) · Methods in molecular biology · doi:10.1007/978-1-62703-140-0_18 · Cited by 8 · see citing works
Singh, M., Borain, J., Noor-Mahomed, N., & Ariatti, M. (2011) · AFRICAN JOURNAL OF BIOTECHNOLOGY · doi:10.5897/ajb10.381 · Cited by 6 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
PEGylation as a strategy for improving nanoparticle-based drug and gene delivery
Suk, J. S., Xu, Q., Kim, N., Hanes, J., & Ensign, L. M. (2016) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2015.09.012 · Cited by 4,523 · see citing works
Self-citationThe current state and future directions of RNAi-based therapeutics
Setten, R. L., Rossi, J. J., & Han, S.-P. (2019) · Nature Reviews Drug Discovery · doi:10.1038/s41573-019-0017-4 · Cited by 1,443 · see citing works
Drug delivery systems for RNA therapeutics
Paunovska, K., Loughrey, D., & Dahlman, J. E. (2022) · Nature Reviews Genetics · doi:10.1038/s41576-021-00439-4 · Cited by 1,349 · see citing works
Targeted therapy in chronic diseases using nanomaterial-based drug delivery vehicles
Singh, A. P., Biswas, A., Shukla, A., & Maiti, P. (2019) · Signal Transduction and Targeted Therapy · doi:10.1038/s41392-019-0068-3 · Cited by 645 · see citing works
Physiology and pathophysiology of human airway mucus
Hill, D. B., Button, B. M., Rubinstein, M., & Boucher, R. C. (2022) · Physiological Reviews · doi:10.1152/physrev.00004.2021 · Cited by 373 · see citing works
Red blood cell-hitchhiking boosts delivery of nanocarriers to chosen organs by orders of magnitude
Brenner, J. S., Pan, D. C., Myerson, J. W., Marcos-Contreras, O. A., Villa, C. H., Patel, P., Hekierski, H., Chatterjee, S., Tao, J., Parhiz, H., et al. (2018) · Nature Communications · doi:10.1038/s41467-018-05079-7 · Cited by 371 · see citing works
Polymer–Lipid Nanoparticles for Systemic Delivery of mRNA to the Lungs
Kaczmarek, J. C., Patel, A. K., Kauffman, K. J., Fenton, O. S., Webber, M. J., Heartlein, M. W., DeRosa, F., & Anderson, D. G. (2016) · Angewandte Chemie International Edition · doi:10.1002/anie.201608450 · Cited by 337 · see citing works
Low ambient humidity impairs barrier function and innate resistance against influenza infection
Kudo, E., Song, E., Yockey, L. J., Rakib, T., Wong, P., Homer, R., & Iwasaki, A. (2019) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1902840116 · Cited by 336 · see citing works
Self-citationThe role of mucus on drug transport and its potential to affect therapeutic outcomes
Murgia, X., Loretz, B., Hartwig, O., Hittinger, M., & Lehr, C. (2017) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2017.10.009 · Cited by 331 · see citing works
Schneider, C. S., Xu, Q., Boylan, N. J., Chisholm, J., Tang, B. C., Schuster, B. S., Henning, A., Ensign, L. M., Lee, E. N., Adstamongkonkul, P., et al. (2017) · Science Advances · doi:10.1126/sciadv.1601556 · Cited by 314 · see citing works
Self-citationPEGylated enhanced cell penetrating peptide nanoparticles for lung gene therapy
Osman, G., Rodriguez, J., Chan, S. Y., Chisholm, J., Duncan, G. A., Kim, N., Tatler, A. L., Shakesheff, K. M., Hanes, J. S., Suk, J. S., et al. (2018) · Journal of Controlled Release · doi:10.1016/j.jconrel.2018.07.001 · Cited by 207 · see citing works
Self-citationPoly(beta-amino ester)s as gene delivery vehicles: challenges and opportunities
Karlsson, J. E., Rhodes, K. R., Green, J. J., & Tzeng, S. Y. (2020) · Expert Opinion on Drug Delivery · doi:10.1080/17425247.2020.1796628 · Cited by 192 · see citing works
Poly(β‐Amino Esters): Synthesis, Formulations, and Their Biomedical Applications
Liu, Y., Li, Y., Keskin, D., & Shi, L. (2018) · Advanced Healthcare Materials · doi:10.1002/adhm.201801359 · Cited by 192 · see citing works
The Mucus Barrier to Inhaled Gene Therapy
Duncan, G. A., Jung, J., Hanes, J. S., & Suk, J. S. (2016) · Molecular Therapy · doi:10.1038/mt.2016.182 · Cited by 189 · see citing works
Self-citationCFTR Modulators: Shedding Light on Precision Medicine for Cystic Fibrosis
Lopes‐Pacheco, M. (2016) · Frontiers in Pharmacology · doi:10.3389/fphar.2016.00275 · Cited by 183 · see citing works
Potential applications and human biosafety of nanomaterials used in nanomedicine
Su, H., Wang, Y., Gu, Y., Bowman, L. L., Zhao, J., & Ding, M. (2017) · Journal of Applied Toxicology · doi:10.1002/jat.3476 · Cited by 179 · see citing works
Selective permeability of mucus barriers
Witten, J. J., Samad, T. S., & Ribbeck, K. (2018) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2018.03.010 · Cited by 171 · see citing works
Mead, B. P., Kim, N.-H., Miller, G. W., Hodges, D., Mastorakos, P., Klibanov, A. L., Mandell, J. W., Hirsh, J., Suk, J. S., Hanes, J. S., et al. (2017) · Nano Letters · doi:10.1021/acs.nanolett.7b00616 · Cited by 162 · see citing works
Self-citationCarlson, T. L., Lock, J. Y., & Carrier, R. L. (2018) · Annual Review of Biomedical Engineering · doi:10.1146/annurev-bioeng-062117-121156 · Cited by 159 · see citing works
Biodegradable Polymers for Gene Delivery
Thomas, T., Tajmir‐Riahi, H., & Pillai, C. K. S. (2019) · Molecules · doi:10.3390/molecules24203744 · Cited by 158 · see citing works
Morales, J. O., Fathe, K., Brunaugh, A. D., Ferrati, S., Li, S., Montenegro-Nicolini, M., Mousavikhamene, Z., McConville, J. T., Prausnitz, M. R., & Smyth, H. D. C. (2017) · The AAPS Journal · doi:10.1208/s12248-017-0054-z · Cited by 155 · see citing works
New and emerging targeted therapies for cystic fibrosis
Quon, B. S., & Rowe, S. M. (2016) · BMJ · doi:10.1136/bmj.i859 · Cited by 148 · see citing works
The particle in the spider's web: transport through biological hydrogels
Witten, J. J., & Ribbeck, K. (2017) · Nanoscale · doi:10.1039/c6nr09736g · Cited by 148 · see citing works
PEGylated PLGA nanoparticles: unlocking advanced strategies for cancer therapy
Kesharwani, P., Kumar, V., Goh, K. W., Gupta, G., Alsayari, A., Wahab, S., & Sahebkar, A. (2025) · Molecular Cancer · doi:10.1186/s12943-025-02410-x · Cited by 132 · see citing works
Delivery methods to increase cellular uptake and immunogenicity of DNA vaccines
Jorritsma, S. H. T., Gowans, E. J., Grubor‐Bauk, B., & Wijesundara, D. K. (2016) · Vaccine · doi:10.1016/j.vaccine.2016.09.062 · Cited by 132 · see citing works
Patented therapeutic drug delivery strategies for targeting pulmonary diseases
Thakur, A. K., Chellappan, D. K., Dua, K., Mehta, M., Satija, S., & Singh, I. (2020) · Expert Opinion on Therapeutic Patents · doi:10.1080/13543776.2020.1741547 · Cited by 132 · see citing works
Protein nanocages that penetrate airway mucus and tumor tissue
Huang, X., Chisholm, J., Zhuang, J., Xiao, Y., Duncan, G., Chen, X., Suk, J. S., & Hanes, J. (2017) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1705407114 · Cited by 131 · see citing works
Self-citationGuan, S., Munder, A., Hedtfeld, S., Braubach, P., Glage, S., Zhang, L., Lienenklaus, S., Schultze, A., Hasenpusch, G., Garrels, W., et al. (2019) · Nature Nanotechnology · doi:10.1038/s41565-018-0358-x · Cited by 130 · see citing works
Nanoparticle Delivery Systems with Cell-Specific Targeting for Pulmonary Diseases
Deng, Z., Kalin, G. T., Shi, D., & Kalinichenko, V. V. (2020) · American Journal of Respiratory Cell and Molecular Biology · doi:10.1165/rcmb.2020-0306tr · Cited by 129 · see citing works
Emerging Therapeutic Approaches for Cystic Fibrosis. From Gene Editing to Personalized Medicine
Pranke, I. M., Golec, A., Hinzpeter, A., Edelman, A., & Sermet‐Gaudelus, I. (2019) · Frontiers in Pharmacology · doi:10.3389/fphar.2019.00121 · Cited by 121 · see citing works
Liu, C., Xi, L. F., Liu, Y., Mak, J., Mao, S., Wang, Z., & Zheng, Y. (2023) · ACS Nano · doi:10.1021/acsnano.3c02075 · Cited by 119 · see citing works
Barriers to inhaled gene therapy of obstructive lung diseases: A review
Kim, N., Duncan, G. A., Hanes, J. S., & Suk, J. S. (2016) · Journal of Controlled Release · doi:10.1016/j.jconrel.2016.05.031 · Cited by 118 · see citing works
Self-citationWang, W., Huang, Z., Huang, Y., Zhang, X., Huang, J., Cui, Y., Xiao, Y., Ma, C., Fu, F., Wang, W., et al. (2022) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2022.114309 · Cited by 117 · see citing works
Krishnamurthy, S., Wohlford-Lenane, C., KANDIMALLA, S., Sartre, G., Meyerholz, D. K., Théberge, V., Hallée, S., Duperré, A.-M., Del’Guidice, T., Lepetit-Stoffaes, J.-P., et al. (2019) · Nature Communications · doi:10.1038/s41467-019-12922-y · Cited by 115 · see citing works
Watchorn, J., Clasky, A. J., Prakash, G., Johnston, I. A. E., Chen, P., & Gu, F. (2022) · ACS Biomaterials Science & Engineering · doi:10.1021/acsbiomaterials.2c00047 · Cited by 114 · see citing works
Gel-forming mucin interactome drives mucus viscoelasticity
Demouveaux, B., Gouyer, V., Gottrand, F., Narita, T., & Desseyn, J. (2017) · Advances in Colloid and Interface Science · doi:10.1016/j.cis.2017.12.005 · Cited by 112 · see citing works
Chen, D., Liu, J., Wu, J., & Suk, J. S. (2020) · Expert Opinion on Drug Delivery · doi:10.1080/17425247.2021.1854222 · Cited by 109 · see citing works
Self-citationSu, L., Li, R., Khan, S. A., Clanton, R., Zhang, F., Lin, Y., Song, Y. H., Wang, H., Fan, J., Hernandez, S., et al. (2018) · Journal of the American Chemical Society · doi:10.1021/jacs.7b11462 · Cited by 108 · see citing works
Recent advances in nanoparticulate RNA delivery systems
Witten, J. J., Hu, Y., Langer, R., & Anderson, D. G. (2024) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2307798120 · Cited by 108 · see citing works
Poly(β-amino ester)-based gene delivery systems: From discovery to therapeutic applications
Cordeiro, R. A., Serra, A. C., Coelho, J. F. J., & Faneca, H. (2019) · Journal of Controlled Release · doi:10.1016/j.jconrel.2019.08.024 · Cited by 106 · see citing works
Organ-on-a-chip technology for nanoparticle research
Kang, S., Park, S. E., & Huh, D. (2021) · Nano Convergence · doi:10.1186/s40580-021-00270-x · Cited by 106 · see citing works
Wang, H., Lu, Q., Zhang, X., Guan, J., & Mao, S. (2022) · Journal of Controlled Release · doi:10.1016/j.jconrel.2022.10.061 · Cited by 88 · see citing works
Polyamine–DNA interactions and development of gene delivery vehicles
Thomas, T., Tajmir‐Riahi, H. A., & Thomas, T. (2016) · Amino Acids · doi:10.1007/s00726-016-2246-8 · Cited by 84 · see citing works
Hypoxia-tropic Protein Nanocages for Modulation of Tumor- and Chemotherapy-Associated Hypoxia
Huang, X., Zhuang, J., Chung, S. W., Huang, B., Halpert, G., Negrón, K., Sun, X., Yang, J., Oh, Y., Hwang, P. M., et al. (2018) · ACS Nano · doi:10.1021/acsnano.8b05399 · Cited by 82 · see citing works
Self-citationDeveloping inhaled protein therapeutics for lung diseases
Matthews, A. A., Ee, P. L. R., & Ge, R. (2020) · Molecular Biomedicine · doi:10.1186/s43556-020-00014-z · Cited by 82 · see citing works
Schneider, C. S., Bhargav, A. G., Perez, J. G., Wadajkar, A. S., Winkles, J. A., Woodworth, G. F., & Kim, A. J. (2015) · Journal of Controlled Release · doi:10.1016/j.jconrel.2015.09.048 · Cited by 81 · see citing works
Continuous Production of Discrete Plasmid DNA‐Polycation Nanoparticles Using Flash Nanocomplexation
Santos, J. L., Ren, Y., Vandermark, J., Archang, M. M., Williford, J., Liu, H., Lee, J., Wang, T., & Mao, H. (2016) · Small · doi:10.1002/smll.201601425 · Cited by 80 · see citing works
Pulmonary siRNA delivery for lung disease: Review of recent progress and challenges
Ding, L., Tang, S., Wyatt, T. A., Knoell, D. L., & Oupický, D. (2020) · Journal of Controlled Release · doi:10.1016/j.jconrel.2020.11.005 · Cited by 79 · see citing works
Conte, C., Mastrotto, F., Taresco, V., Tchoryk, A., Quaglia, F., Stolnik, S., & Alexander, C. (2018) · Journal of Controlled Release · doi:10.1016/j.jconrel.2018.03.011 · Cited by 75 · see citing works
das Neves, J., Arzi, R. S., & Sosnik, A. (2020) · Chemical Society Reviews · doi:10.1039/c8cs00948a · Cited by 72 · see citing works
New perspectives in nanotherapeutics for chronic respiratory diseases
da Silva, A. L., Cruz, F. F., Rocco, P. R. M., & Morales, M. M. (2017) · Biophysical Reviews · doi:10.1007/s12551-017-0319-x · Cited by 72 · see citing works
Self-citationQin, M., Zhang, J., Li, M., Yang, D., Liu, D., Song, S., Fu, J., Zhang, H., Dai, W., Wang, X., et al. (2020) · Theranostics · doi:10.7150/thno.38900 · Cited by 70 · see citing works
Poly-beta-amino-esters nano-vehicles based drug delivery system for cartilage
Perni, S., & Prokopovich, P. (2016) · Nanomedicine Nanotechnology Biology and Medicine · doi:10.1016/j.nano.2016.10.001 · Cited by 69 · see citing works
Wei, Y., Li, X., Lin, J., Zhou, Y., Yang, J., Hou, M., Wu, F., Yan, J., Ge, C., Hu, D., et al. (2022) · Advanced Materials · doi:10.1002/adma.202206821 · Cited by 65 · see citing works
Newby, J., Seim, I., Lysy, M., Ling, Y., Huckaby, J. T., Lai, S. K., & Forest, M. G. (2017) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2017.12.002 · Cited by 63 · see citing works
Delivering on the promise of gene editing for cystic fibrosis
Hodges, C. A., & Conlon, R. A. (2018) · Genes & Diseases · doi:10.1016/j.gendis.2018.11.005 · Cited by 62 · see citing works
Delivery technologies for in utero gene therapy
Palanki, R., Peranteau, W. H., & Mitchell, M. J. (2021) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2020.11.002 · Cited by 64 · see citing works
Yang, J., Duan, S., Ye, H., Ge, C., Piao, C., Chen, Y., Lee, M., & Yin, L. (2021) · Advanced Functional Materials · doi:10.1002/adfm.202008960 · Cited by 61 · see citing works
Nebulised delivery of RNA formulations to the lungs: From aerosol to cytosol
Neary, M. T., Mulder, L. M., Kowalski, P. S., MacLoughlin, R. J., Crean, A. M., & Ryan, K. B. (2024) · Journal of Controlled Release · doi:10.1016/j.jconrel.2023.12.012 · Cited by 62 · see citing works
Wilson, D. R., Mosenia, A., Suprenant, M. P., Upadhya, R., Routkevitch, D., Meyer, R. A., Quiñones‐Hinojosa, A., & Green, J. J. (2017) · Journal of Biomedical Materials Research Part A · doi:10.1002/jbm.a.36033 · Cited by 61 · see citing works
Polymer–Lipid Nanoparticles for Systemic Delivery of mRNA to the Lungs
Kaczmarek, J. C., Patel, A. K., Kauffman, K. J., Fenton, O. S., Webber, M. J., Heartlein, M. W., DeRosa, F., & Anderson, D. G. (2016) · Angewandte Chemie · doi:10.1002/ange.201608450 · Cited by 60 · see citing works
An Adeno-Associated Viral Vector Capable of Penetrating the Mucus Barrier to Inhaled Gene Therapy
Duncan, G. A., Kim, N., Colon-Cortes, Y., Rodriguez, J., Mazur, M., Birket, S. E., Rowe, S. M., West, N. E., Livraghi-Butrico, A., Boucher, R. C., et al. (2018) · Molecular Therapy — Methods & Clinical Development · doi:10.1016/j.omtm.2018.03.006 · Cited by 60 · see citing works
Self-citationBiodegradable brain-penetrating DNA nanocomplexes and their use to treat malignant brain tumors
Mastorakos, P., Zhang, C., Song, E., Kim, Y., Park, H. W., Berry, S., Choi, W. K., Hanes, J. S., & Suk, J. S. (2017) · Journal of Controlled Release · doi:10.1016/j.jconrel.2017.07.009 · Cited by 59 · see citing works
Self-citationKates, M. R., Date, A. A., Yoshida, T., Afzal, U., Kanvinde, P. P., Babu, T., Sopko, N. A., Matsui, H., Hahn, N. M., McConkey, D. J., et al. (2017) · Clinical Cancer Research · doi:10.1158/1078-0432.ccr-17-1082 · Cited by 59 · see citing works
Self-citationZhu, D., Shen, H., Tan, S., Hu, Z., Wang, L., Yu, L., Tian, X., Ding, W., Ren, C., Gao, C., et al. (2018) · Molecular Therapy · doi:10.1016/j.ymthe.2018.07.019 · Cited by 59 · see citing works
Novel Perspectives towards RNA-Based Nano-Theranostic Approaches for Cancer Management
Arshad, R., Fatima, I., Sargazi, S., Rahdar, A., Karamzadeh-Jahromi, M., Pandey, S., Diez-Pascual, A. M., & Bilal, M. (2021) · Nanomaterials · doi:10.3390/nano11123330 · Cited by 58 · see citing works
Lipid-Based Vectors for Therapeutic mRNA-Based Anti-Cancer Vaccines
Guevara, M. L., Persano, S., & Persano, F. (2019) · Current Pharmaceutical Design · doi:10.2174/1381612825666190619150221 · Cited by 56 · see citing works
Inhaled immunoantimicrobials for the treatment of chronic obstructive pulmonary disease
Zhu, J., Li, X., Zhou, Y., Ge, C., Li, X., Hou, M., Wei, Y., Chen, Y., Leong, K. W., & Yin, L. (2024) · Science Advances · doi:10.1126/sciadv.abd7904 · Cited by 54 · see citing works
Biodegradable DNA Nanoparticles that Provide Widespread Gene Delivery in the Brain
Mastorakos, P., Song, E., Zhang, C., Berry, S., Park, H. W., Kim, Y., Park, J., Lee, S., Suk, J. S., & Hanes, J. S. (2015) · Small · doi:10.1002/smll.201502554 · Cited by 51 · see citing works
Self-citationHoang, T., Zierden, H. C., Date, A. A., Ortiz, J., Gumber, S., Anders, N. M., He, P., Segars, J. H., Hanes, J. S., Mahendroo, M., et al. (2018) · Journal of Controlled Release · doi:10.1016/j.jconrel.2018.12.046 · Cited by 51 · see citing works
Self-citationThe potential for targeted rewriting of epigenetic marks in COPD as a new therapeutic approach
Wu, D., Song, J., Bartel, S., Krauss‐Etschmann, S., Rots, M. G., & Hylkema, M. N. (2017) · Pharmacology & Therapeutics · doi:10.1016/j.pharmthera.2017.08.007 · Cited by 49 · see citing works
Inhaled RNA drugs to treat lung diseases: Disease-related cells and nano–bio interactions
Zhang, M., Lu, H., Xie, L., Liu, X., Cun, D., & Yang, M. (2023) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2023.115144 · Cited by 48 · see citing works
He, S., Fang, J., Zhong, C., Wang, M., & Ren, F. (2022) · Advanced Healthcare Materials · doi:10.1002/adhm.202201096 · Cited by 47 · see citing works
Majumder, J., & Minko, T. (2021) · Pharmaceutics · doi:10.3390/pharmaceutics13071063 · Cited by 47 · see citing works
Gene Editing and Genetic Lung Disease. Basic Research Meets Therapeutic Application
Alapati, D., & Morrisey, E. E. (2016) · American Journal of Respiratory Cell and Molecular Biology · doi:10.1165/rcmb.2016-0301ps · Cited by 46 · see citing works
da Silva, A. L., Oliveira, G. P., Kim, N., Cruz, F. F., Kitoko, J. Z., Blanco, N. G., Martini, S. V., Hanes, J. S., Rocco, P. R. M., Suk, J. S., et al. (2020) · Science Advances · doi:10.1126/sciadv.aay7973 · Cited by 46 · see citing works
Self-citationBiomaterial-based nanoreactors, an alternative for enzyme delivery
Koyani, R. D., Pérez‐Robles, J., Cadena-Nava, R. D., & Vázquez-Duhalt, R. (2017) · Nanotechnology Reviews · doi:10.1515/ntrev-2016-0071 · Cited by 45 · see citing works
Iqbal, S., Qu, Y., Dong, Z., Zhao, J., Khan, A. R., Rehman, S., & Zhao, Z. (2020) · European Polymer Journal · doi:10.1016/j.eurpolymj.2020.110097 · Cited by 45 · see citing works
Advances in nanotechnology and asthma
Wang, L., Feng, M., Li, Q., Qiu, C., & Chen, R. (2019) · Annals of Translational Medicine · doi:10.21037/atm.2019.04.62 · Cited by 44 · see citing works
Advanced human-relevant in vitro pulmonary platforms for respiratory therapeutics
Artzy‐Schnirman, A., Raviv, S. A., Doppelt‐Flikshtain, O., Shklover, J., Korin, N., Gross, A., Mizrahi, B., Schroeder, A., & Sznitman, J. (2021) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2021.113901 · Cited by 43 · see citing works
Lessons learned from lung and liver in-vivo gene therapy: implications for the future
van Haasteren, J., Hyde, S. C., & Gill, D. R. (2018) · Expert Opinion on Biological Therapy · doi:10.1080/14712598.2018.1506761 · Cited by 40 · see citing works
Black, K. C. L., Ibricevic, A., Gunsten, S. P., Flores, J. A., Gustafson, T. P., Raymond, J. E., Samarajeewa, S., Shrestha, R., Felder, S. E., Cai, T., et al. (2016) · Biomaterials · doi:10.1016/j.biomaterials.2016.04.040 · Cited by 39 · see citing works
Alford, A., Tucker, B. A., Kozlovskaya, V., Chen, J., Gupta, N., Caviedes, R., Gearhart, J., Graves, D. E., & Kharlampieva, E. (2018) · Polymers · doi:10.3390/polym10121342 · Cited by 39 · see citing works
Genetic therapies for cystic fibrosis lung disease
Hart, S. L., & Harrison, P. T. (2017) · Current Opinion in Pharmacology · doi:10.1016/j.coph.2017.10.006 · Cited by 37 · see citing works
Almeida, A. P. B., Damaceno, G. B. R., Carneiro, A. F., Bohr, A., Gonçalves, H. R., Valadares, M. C., Nascimento, T. L., & Lima, E. M. (2019) · Journal of Biomaterials Applications · doi:10.1177/0885328219863291 · Cited by 36 · see citing works
Gene therapy for Cystic Fibrosis: recent advances and future prospects
Lomunova, M. A., & Михайлович, Г. П. (2023) · Acta Naturae · doi:10.32607/actanaturae.11708 · Cited by 35 · see citing works
Inhalable materials and biologics for lung defence and drug delivery
Zhang, S. W., Edwards, D. A., Langer, R., & Cheng, K. (2025) · Nature Reviews Materials · doi:10.1038/s41578-025-00841-y · Cited by 37 · see citing works
Enhancing dendritic cell activation and HIV vaccine effectiveness through nanoparticle vaccination
Glass, J. J., Kent, S. J., & Rose, R. D. (2016) · Expert Review of Vaccines · doi:10.1586/14760584.2016.1141054 · Cited by 34 · see citing works
Gene delivery to the lungs: pulmonary gene therapy for cystic fibrosis
Villate-Beitia, I., Sesma, J. Z., Puras, G., & Pedraz, J. L. (2017) · Drug Development and Industrial Pharmacy · doi:10.1080/03639045.2017.1298122 · Cited by 33 · see citing works
Epigenetic Mechanisms in Parenchymal Lung Diseases: Bystanders or Therapeutic Targets?
Avcı, E., Sarvari, P., Savai, R., Seeger, W., & Pullamsetti, S. S. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms23010546 · Cited by 33 · see citing works
Chisholm, J., Shenoy, S., Shade, J. K., Kim, V., Putcha, N., Carson, K. A., Wise, R. A., Hansel, N. N., Hanes, J. S., Suk, J. S., et al. (2019) · European Respiratory Journal · doi:10.1183/13993003.00088-2019 · Cited by 32 · see citing works
Self-citationSpray drying: Inhalable powders for pulmonary gene therapy
Munir, M., Jena, L., Kett, V. L., Dunne, N., & McCarthy, H. O. (2021) · Biomaterials Advances · doi:10.1016/j.msec.2021.112601 · Cited by 32 · see citing works
Carneiro, S. P., Greco, A., Chiesa, E., Genta, I., & Merkel, O. M. (2023) · Expert Opinion on Drug Delivery · doi:10.1080/17425247.2023.2185220 · Cited by 31 · see citing works
Functional Nanocarrier for Drug and Gene Delivery Via Local Administration in Mucosal Tissues
Wang, L., Zhou, Y., Wu, M., Wu, M., Li, X., Gong, X., Chang, J. K., & Zhang, X. (2017) · Nanomedicine · doi:10.2217/nnm-2017-0143 · Cited by 31 · see citing works
Advances in Nonviral mRNA Delivery Materials and Their Application as Vaccines for Melanoma Therapy
Neill, B., Romero, A. R., & Fenton, O. S. (2023) · ACS Applied Bio Materials · doi:10.1021/acsabm.3c00721 · Cited by 30 · see citing works
Magnetic wire active microrheology of human respiratory mucus
Radiom, M., Hénault, R., Mani, S., Grein-Iankovski, A., Norel, X., & Berret, J. (2021) · Soft Matter · doi:10.1039/d1sm00512j · Cited by 29 · see citing works
Iqbal, S., & Zhao, Z. (2021) · International Journal of Pharmaceutics · doi:10.1016/j.ijpharm.2021.121289 · Cited by 29 · see citing works
Engineering of pulmonary surfactant corona on inhaled nanoparticles to operate in the lung system
Falahati, M., Hasan, A., Zeinabad, H. A., Serpooshan, V., von der Thüsen, J. H., & ten Hagen, T. L. M. (2023) · Nano Today · doi:10.1016/j.nantod.2023.101998 · Cited by 28 · see citing works
Chen, D., Liu, S., Chen, D., Liu, J., Wu, J., Wang, H., Su, Y., Kwak, G., Zuo, X., Rao, D., et al. (2021) · Angewandte Chemie International Edition · doi:10.1002/anie.202101732 · Cited by 28 · see citing works
Self-citationPoly (beta‐amino ester) as an in vivo nanocarrier for therapeutic nucleic acids
Nezhad, M. S. (2022) · Biotechnology and Bioengineering · doi:10.1002/bit.28269 · Cited by 28 · see citing works
- Authors
- Mastorakos, P., da Silva, A. L., Chisholm, J., Song, E., Choi, W. K., Boyle, M. P., Morales, M. M., Hanes, J., & Suk, J. S.
- Affiliations
- Johns Hopkins University (US)
- Laboratory of Cellular and Molecular Physiology, Carlos Chagas Filho Institute of Biophysics, Federal University of Rio de Janeiro, Rio de Janeiro, RJ 21941902, Brazil;
- Universidade Federal do Rio de Janeiro (BR)
- ORCID
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 2015-06-29
- Volume
- 112
- Issue
- 28
- Pages
- 8720-8725
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC4507234
- OpenAlex
- W2149239077
- Cited by
- 210
- References
- 66
- Citations by year
- 2026: 15 · 2025: 13 · 2024: 20 · 2023: 20 · 2022: 17 · 2021: 24 · 2020: 22 · 2019: 21 · 2018: 20 · 2017: 20 · 2016: 15 · 2015: 3
- FWCI
- 8.40
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- HHS | National Institutes of Health (P01 HL51811, R01 HL127413)
- Cystic Fibrosis Foundation (HANES07XX0, HANES15G0)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Inhalation and Respiratory Drug Delivery
- Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
- Keywords
- DNA nanoparticles, pulmonary gene therapy, mucus barrier, poly(β-amino ester), biodegradable polymers, transgene expression, nanoparticle stability, intratracheal administration, nanoparticle toxicity
- MeSH terms
- Administration, Inhalation, Animals, Cystic Fibrosis, DNA, Mucus, Genetic Therapy, Mice, Nanoparticles
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review