Protein kinase C delta phosphorylates ecdysone receptor B1 to promote gene expression and apoptosis under 20-hydroxyecdysone regulation
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Significance: The steroid hormone 20-hydroxyecdysone (20E) promotes apoptosis during larval-to-adult metamorphosis in insects. However, the mechanisms governing this process are unclear. This work reveals that 20E up-regulates the expression of the protein kinase PKCδ isoform E. Overexpression of the catalytic domain of PKCδ is sufficient to increase caspase-3 activity and apoptosis. PKCδ directly phosphorylates a threonine residue at position 468 of the amino acid sequence of nuclear receptor EcRB1. The phosphorylation of EcRB1 is essential for apoptotic gene transcription. These results demonstrate the mechanism by which the steroid hormone 20E promotes PKCδ expression to regulate apoptosis.
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The genomic action/pathway of 20E is regulated by the nongenomic action/pathway because the 20E-induced rapid calcium increase in cells activates protein kinases, thereby promoting EcR-USP transcription complex formation, which initiates gene transcription in the 20E pathway.
“From the studies in H. armigera, the genomic action/pathway of 20E is regulated by the nongenomic action/pathway, because the 20E-induced rapid calcium increase in cells activates protein kinases [52, 56], therefore promotes EcR-USP transcription complex formation, which initiates gene transcription in 20E pathway finally [64].”
From G protein-coupled receptors function as cell membrane receptors for the steroid hormone 20-hydroxyecdysone (2020) · cites this paper as [64]
20E regulates gene transcription via its nuclear receptor EcR in H. armigera.
“20E regulates gene transcription via its nuclear receptor EcR in H. armigera [64].”
From G protein-coupled receptors function as cell membrane receptors for the steroid hormone 20-hydroxyecdysone (2020) · cites this paper as [64]
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- Authors
- Chen, C.-H., Pan, J., Di, Y.-Q., Liu, W., Hou, L., Wang, J.-X., & Zhao, X.-F.
- Affiliations
- Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Sciences, Shandong University, Jinan 250100, China
- Shandong University (CN)
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 2017-08-08
- Volume
- 114
- Issue
- 34
- Pages
- E7121-E7130
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC5576805
- OpenAlex
- W2745179998
- Cited by
- 45
- References
- 46
- Citations by year
- 2026: 5 · 2025: 5 · 2024: 4 · 2023: 7 · 2022: 8 · 2021: 5 · 2020: 3 · 2019: 6 · 2018: 2
- FWCI
- 1.23
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China (31230067, 31572328)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Estrogen and related hormone effects
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Cell death mechanisms and regulation
- Keywords
- protein kinase C delta, 20-hydroxyecdysone, ecdysone response element, USP1, apoptosis, caspase-3, threonine phosphorylation, gene transcription, nuclear localization
- MeSH terms
- Animals, Cell Nucleus, Ecdysterone, Larva, Metamorphosis, Biological, Moths, Phosphorylation, Receptors, Steroid, Apoptosis, Gene Expression Regulation, Developmental, Insect Proteins, Response Elements, Protein Kinase C-delta, Ubiquitin-Specific Proteases
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