G-protein αq participates in the steroid hormone 20-hydroxyecdysone nongenomic signal transduction
Abstract
The nuclear receptor-mediated genomic pathways of the animal steroid hormones are well known. However, the cell membrane receptor-mediated nongenomic pathways of the animal steroid hormones are little understood. In this study, we report the participation of a G-protein alpha q (Gαq)(1) subunit in the 20E nongenomic pathway in the cell membrane and regulating gene expression during molting and metamorphosis in a lepidopteran insect, Helicoverpa armigera. 20E-induced phosphorylation of Gαq was detected using two-dimensional electrophoresis techniques. Knockdown of Gαq by injecting double-stranded RNA suppressed the development of larvae, delayed metamorphosis, and inhibited 20E-induced gene expression. Gαq was distributed throughout the cell, and migrated toward the plasma membrane upon 20E induction. Gαq was necessary in the 20E-induced intracellular Ca(2+) release and extracellular Ca(2+) influx. The protein kinase C (PKC) inhibitor could repress 20E-induced phosphorylation of cyclin-dependent kinase 10 (CDK10) and transcription factor ultraspiracle (USP1). PKC inhibitor could repress the Gαq phosphorylation and membrane trafficking. These results suggest that Gαq participates in 20E signaling in the cell membrane at the pre-genomic stage by modulating the increase of the intracellular Ca(2+) and phosphorylation of CDK10 and USP1 in 20E transcription complex to regulate gene transcription.
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In H. armigera, phosphorylation of G protein alpha q subunit (Gαq) is induced by 20E.
“In H. armigera, the phosphorylation of G protein alpha q subunit (Gαq) is induced by 20E [50].”
From G protein-coupled receptors function as cell membrane receptors for the steroid hormone 20-hydroxyecdysone (2020) · cites this paper as [50]
Gαq participates in the 20E-induced increase in intracellular Ca2+ levels and is necessary for larval development, metamorphosis, and 20E pathway gene expression, and plays roles downstream of ErGPCR-1 in 20E signaling.
“Gαq participates in the 20E-induced increase in intracellular Ca2+ levels and is necessary for larval development, metamorphosis, and 20E pathway gene expression, and plays roles downstream of ErGPCR-1 in 20E signaling [50].”
From G protein-coupled receptors function as cell membrane receptors for the steroid hormone 20-hydroxyecdysone (2020) · cites this paper as [50]
Cites
Bradford, M. M. (1976) · Analytical Biochemistry · doi:10.1016/0003-2697(76)90527-3 · Cited by 255,302 · see citing works
Analyzing real-time PCR data by the comparative CT method
Schmittgen, T. D., & Livak, K. J. (2008) · Nature Protocols · doi:10.1038/nprot.2008.73 · Cited by 26,190 · see citing works
G PROTEINS: TRANSDUCERS OF RECEPTOR-GENERATED SIGNALS
Gilman, A. G. (1987) · Annual Review of Biochemistry · doi:10.1146/annurev.bi.56.070187.003151 · Cited by 6,405 · see citing works
Clapham, D. E. (2007) · Cell · doi:10.1016/j.cell.2007.11.028 · Cited by 4,175 · see citing works
Regulation of Phosphoinositide-Specific Phospholipase C
Rhee, S. G. (2001) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.70.1.281 · Cited by 1,628 · see citing works
The extended protein kinase C superfamily
MELLOR, H., & Parker, P. J. (1998) · Biochemical Journal · doi:10.1042/bj3320281 · Cited by 1,539 · see citing works
Filardo, E. J., Quinn, J. A., Bland, K. I., & Frackelton, A. R. (2000) · Molecular Endocrinology · doi:10.1210/mend.14.10.0532 · Cited by 1,387 · see citing works
G-protein-coupled receptors and signaling networks: emerging paradigms
Marinissen, M. J., & Gutkind, J. S. (2001) · Trends in Pharmacological Sciences · doi:10.1016/s0165-6147(00)01678-3 · Cited by 1,049 · see citing works
Koelle, M. R., Talbot, W. S., Segraves, W. A., Bender, M. T., Cherbas, P. T., & Hogness, D. S. (1991) · Cell · doi:10.1016/0092-8674(91)90572-g · Cited by 978 · see citing works
Regulation of Phosphoinositide-specific Phospholipase C Isozymes
Rhee, S. G., & Bae, Y. S. (1997) · Journal of Biological Chemistry · doi:10.1074/jbc.272.24.15045 · Cited by 876 · see citing works
Riddiford, L. M., Hiruma, K., Zhou, X., & Nelson, C. A. (2003) · Insect Biochemistry and Molecular Biology · doi:10.1016/j.ibmb.2003.06.001 · Cited by 547 · see citing works
Protein kinase C: poised to signal
Newton, A. C. (2009) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00477.2009 · Cited by 520 · see citing works
Galès, C., Durm, J. J. J. V., Schaak, S., Pontier, S. M., Percherancier, Y., Audet, M., Paris, H., & Bouvier, M. (2006) · Nature Structural & Molecular Biology · doi:10.1038/nsmb1134 · Cited by 461 · see citing works
MOLECULAR MECHANISMS OF STEROID HORMONE SIGNALING IN PLANTS
Vert, G., Nemhauser, J. L., Geldner, N., Hong, F., & Chory, J. (2005) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev.cellbio.21.090704.151241 · Cited by 404 · see citing works
Hormonal cross talk in insect development
Dubrovsky, E. B. (2004) · Trends in Endocrinology and Metabolism · doi:10.1016/j.tem.2004.11.003 · Cited by 362 · see citing works
Zhou, X., & Riddiford, L. M. (2002) · Development · doi:10.1242/dev.129.9.2259 · Cited by 336 · see citing works
Srivastava, D. P., Yu, E. J., Kennedy, K., Chatwin, H., Reale, V., Hamon, M., Smith, T., & Evans, P. D. (2005) · The Journal of Neuroscience · doi:10.1523/jneurosci.1005-05.2005 · Cited by 272 · see citing works
Hormone Receptors and the Regulation of Insect Metamorphosis
Riddiford, L. M., & Truman, J. W. (1993) · American Zoologist · doi:10.1093/icb/33.3.340 · Cited by 258 · see citing works
Signal Perception and Transduction: The Origin of the Phenotype.
TREWAVAS, A. J., & Malhó, R. (1997) · The Plant Cell · doi:10.1105/tpc.9.7.1181 · Cited by 257 · see citing works
Functions and Regulatory Mechanisms of Gq-Signaling Pathways
Mizuno, N., & Itoh, H. (2009) · Neurosignals · doi:10.1159/000186689 · Cited by 181 · see citing works
Chmura, S. J., Dolan, M. E., Cha, A., Mauceri, H. J., Kufe, D. W., & Weichselbaum, R. R. (2000) · PubMed · Cited by 173 · see citing works
Steroid signaling in plants and insects—common themes, different pathways
Thummel, C. S., & Chory, J. (2002) · Genes & Development · doi:10.1101/gad.1042102 · Cited by 161 · see citing works
Structural features of the G-protein/GPCR interactions
Moreira, I. S. (2013) · Biochimica et Biophysica Acta (BBA) - General Subjects · doi:10.1016/j.bbagen.2013.08.027 · Cited by 113 · see citing works
Lan, Q., Hiruma, K., Hu, X., Jindra, M., & Riddiford, L. M. (1999) · Molecular and Cellular Biology · doi:10.1128/mcb.19.7.4897 · Cited by 107 · see citing works
Non-canonical signaling and localizations of heterotrimeric G proteins
Hewavitharana, T., & Wedegaertner, P. (2011) · Cellular Signalling · doi:10.1016/j.cellsig.2011.08.014 · Cited by 100 · see citing works
Gorelick-Feldman, J., Cohick, W., & Raskin, I. (2010) · Steroids · doi:10.1016/j.steroids.2010.03.008 · Cited by 96 · see citing works
Ishimoto, H., Wang, Z., Rao, Y., Wu, C., & Kitamoto, T. (2013) · PLoS Genetics · doi:10.1371/journal.pgen.1003843 · Cited by 81 · see citing works
Zhao, X., Wang, J., & Wang, Y.-C. (1998) · Insect Biochemistry and Molecular Biology · doi:10.1016/s0965-1748(98)00015-0 · Cited by 80 · see citing works
Self-citationLounsbury, K. M., Schlegel, B. P., Poncz, M., Brass, L. F., & Manning, D. R. (1993) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)53721-2 · Cited by 79 · see citing works
Establishment of a New Cell Line from Lepidopteran Epidermis and Hormonal Regulation on the Genes
Shao, H., Zheng, W., Liu, P., Wang, Q., Wang, J., & Zhao, X. (2008) · PLoS ONE · doi:10.1371/journal.pone.0003127 · Cited by 79 · see citing works
Self-citationDeregt, D., Bolin, S. R., van den Hurk, J., Ridpath, J. F., & Gilbert, S. A. (1998) · Virus Research · doi:10.1016/s0168-1702(97)00129-9 · Cited by 61 · see citing works
Liu, W., Cai, M.-J., Zheng, C.-C., Wang, J.-X., & Zhao, X.-F. (2014) · Journal of Biological Chemistry · doi:10.1074/jbc.m113.547018 · Cited by 59 · see citing works
Self-citationNongenomic action of an insect steroid hormone in steroid-induced programmed cell death
Iga, M., Iwami, M., & Sakurai, S. (2006) · Molecular and Cellular Endocrinology · doi:10.1016/j.mce.2006.08.005 · Cited by 59 · see citing works
Vines, C. M. (2012) · Advances in experimental medicine and biology · doi:10.1007/978-94-007-2888-2_10 · Cited by 52 · see citing works
Heterotrimeric G Protein Signaling Outside the Realm of Seven Transmembrane Domain Receptors
Marty, C., & Ye, R. D. (2010) · Molecular Pharmacology · doi:10.1124/mol.110.063453 · Cited by 51 · see citing works
G-protein-coupled receptor participates in 20-hydroxyecdysone signaling on the plasma membrane
Cai, M.-J., Dong, D.-J., Wang, Y., Liu, P.-C., Liu, W., Wang, J.-X., & Zhao, X.-F. (2014) · Cell Communication and Signaling · doi:10.1186/1478-811x-12-9 · Cited by 44 · see citing works
Self-citationLiu, W., Cai, M.-J., Wang, J.-X., & Zhao, X.-F. (2014) · Endocrinology · doi:10.1210/en.2013-2020 · Cited by 38 · see citing works
Self-citationPKC-Mediated USP Phosphorylation at Ser35 Modulates 20-Hydroxyecdysone Signaling in Drosophila
Wang, S., Wang, J., Sun, Y., Song, Q., & Li, S. (2012) · Journal of Proteome Research · doi:10.1021/pr3008804 · Cited by 38 · see citing works
Self-citationManaboon, M., Iga, M., Iwami, M., & Sakurai, S. (2009) · Journal of Insect Physiology · doi:10.1016/j.jinsphys.2008.10.013 · Cited by 37 · see citing works
Schlattner, U., Vafopoulou, X., Steel, C. G. H., Hormann, R. E., & Lezzi, M. (2006) · Molecular and Cellular Endocrinology · doi:10.1016/j.mce.2005.12.051 · Cited by 37 · see citing works
Nicolaı̈, M., Bouhin, H., Quennedey, B., & Delachambre, J. (2000) · Insect Molecular Biology · doi:10.1046/j.1365-2583.2000.00181.x · Cited by 36 · see citing works
Wang, J., Jiang, X., Wang, Q., Hou, L., Xu, D., Wang, J., & Zhao, X. (2007) · BMC Developmental Biology · doi:10.1186/1471-213x-7-76 · Cited by 35 · see citing works
Self-citationLiu, C.-Y., Liu, W., Zhao, W., Wang, J., & Zhao, X.-F. (2012) · APOPTOSIS · doi:10.1007/s10495-012-0784-4 · Cited by 33 · see citing works
Self-citationSui, Y., Wang, J., & Zhao, X. (2009) · Insect Molecular Biology · doi:10.1111/j.1365-2583.2009.00884.x · Cited by 32 · see citing works
Self-citationAdjobo-Hermans, M. J. W., Crosby, K. C., Putyrski, M., Bhageloe, A., van Weeren, L., Schultz, C., Goedhart, J., & Gadella, T. W. J. (2012) · Cellular Signalling · doi:10.1016/j.cellsig.2012.09.022 · Cited by 32 · see citing works
Liu, P., Wang, J., Song, Q., & Zhao, X. (2011) · PLoS ONE · doi:10.1371/journal.pone.0019776 · Cited by 31 · see citing works
Self-citation20E-regulated USP expression and phosphorylation in Drosophila melanogaster
Song, Q., Sun, X., & Jin, X. (2003) · Insect Biochemistry and Molecular Biology · doi:10.1016/j.ibmb.2003.06.005 · Cited by 30 · see citing works
G protein inactive and active forms investigated by simulation methods
Хафизов, К. Ф., Lattanzi, G., & Carloni, P. (2008) · Proteins Structure Function and Bioinformatics · doi:10.1002/prot.22303 · Cited by 26 · see citing works
Steroid signal transduction activated at the cell membrane: from plants to animals.
Marcinkowska, E., & Więdłocha, A. (2002) · Acta Biochimica Polonica · doi:10.18388/abp.2002_3782 · Cited by 22 · see citing works
Hou, L., Cai, M., Liu, W., Song, Q., & Zhao, X. (2012) · Developmental Biology · doi:10.1016/j.ydbio.2012.06.015 · Cited by 20 · see citing works
Self-citationFifty years ago: The quest for steroid hormone receptors
Rousseau, G. G. (2013) · Molecular and Cellular Endocrinology · doi:10.1016/j.mce.2013.05.005 · Cited by 18 · see citing works
Díaz‐Troya, S., Najib, S., & Sánchez‐Margalet, V. (2004) · Regulatory Peptides · doi:10.1016/j.regpep.2004.07.031 · Cited by 15 · see citing works
The knockdown of Ha-GRIM-19 by RNA interference induced programmed cell death
Dong, D.-J., Liu, P., Wang, J., & Zhao, X. (2010) · Amino Acids · doi:10.1007/s00726-010-0824-8 · Cited by 11 · see citing works
Self-citationDowney, P. M., Lozza, G., Petrò, R., Diodato, E., Foglia, C., Bottazzoli, F., Brusa, R., Asquini, T., Reggiani, A. M., & Grilli, M. (2005) · SLAS DISCOVERY · doi:10.1177/1087057105280285 · Cited by 10 · see citing works
[Intra- and intercellular Ca(2+)-signal transduction].
Himpens, B., & Vereecke, J. (2000) · PubMed · Cited by 8 · see citing works
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Cited by
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Kang, X.-L., Zhang, J.-Y., Wang, D., Zhao, Y.-M., Han, X.-L., Wang, J.-X., & Zhao, X.-F. (2019) · PLOS Genetics · doi:10.1371/journal.pgen.1008331 · Cited by 96 · see citing works
Self-citationZhao, X.-F. (2020) · Cell Communication and Signaling · doi:10.1186/s12964-020-00620-y · Cited by 60 · see citing works
Self-citationChen, C.-H., Pan, J., Di, Y.-Q., Liu, W., Hou, L., Wang, J.-X., & Zhao, X.-F. (2017) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1704999114 · Cited by 45 · see citing works
Self-citationJing, Y.-P., Liu, W., Wang, J.-X., & Zhao, X.-F. (2015) · Journal of Biological Chemistry · doi:10.1074/jbc.m114.622696 · Cited by 30 · see citing works
Self-citationJing, Y.-P., Wang, D., Han, X.-L., Dong, D.-J., Wang, J.-X., & Zhao, X.-F. (2016) · Journal of Biological Chemistry · doi:10.1074/jbc.m115.706028 · Cited by 30 · see citing works
Self-citationLOOF, A. D., & Schoofs, L. (2019) · Frontiers in Neuroscience · doi:10.3389/fnins.2019.00141 · Cited by 24 · see citing works
Evolution of non-genomic nuclear receptor function
Taylor, E., & Heyland, A. (2021) · Molecular and Cellular Endocrinology · doi:10.1016/j.mce.2021.111468 · Cited by 21 · see citing works
Baev, A. Y., Charishnikova, O. S., Khasanov, F. A., Nebesnaya, K. S., Makhmudov, A. R., Rakhmedova, M. T., Khushbaktova, Z. A., Syrov, V. N., & Levitskaya, Y. V. (2022) · The Journal of Steroid Biochemistry and Molecular Biology · doi:10.1016/j.jsbmb.2022.106066 · Cited by 16 · see citing works
Zhao, X. (2020) · Journal of Integrative Agriculture · doi:10.1016/s2095-3119(19)62860-1 · Cited by 14 · see citing works
Self-citationLOOF, A. D., & Schoofs, L. (2019) · Frontiers in Neuroscience · doi:10.3389/fnins.2019.00465 · Cited by 5 · see citing works
Zhao, X., Dong, Q., Zhu, H., Ding, Y., Deng, D., Miao, H., Tan, Y., & Ge, L. (2025) · Pest Management Science · doi:10.1002/ps.8722 · Cited by 3 · see citing works
Self-citationEffects of dietary 20-hydroxyecdysone supplementation on whole-body protein turnover in growing pigs
Obvintseva, O. V., Erimbetov, K. T., & Mikhailov, V. (2020) · E3S Web of Conferences · doi:10.1051/e3sconf/202017503008 · Cited by 2 · see citing works
Tan, Y., Zhao, X., Zhao, J., Ji, Q.-Q., Xiao, L., & Hao, D.-J. (2020) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2020.10.15.341115
Self-citationФедорова, А. В., Erimbetov, K. T., Анатольевна, Д. Л., Izmestieva, O. S., & Sofronova, O. (2020) · Genetics and breeding of animals · doi:10.31043/2410-2733-2020-3-114-121
Tan, Y., Zhao, X., Zhao, J., Zhu‐Salzman, K., Ji, Q.-Q., Xiao, L., & Hao, D.-J. (2022) · Frontiers in Physiology · doi:10.3389/fphys.2022.845087
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- Authors
- Ren, J., Li, X.-R., Liu, P.-C., Cai, M.-J., Liu, W., Wang, J.-X., & Zhao, X.-F.
- Affiliations
- Shandong University (CN)
- Corresponding author
- Zhao, Xiao-Fan
- Venue
- The Journal of Steroid Biochemistry and Molecular Biology
- Publisher
- Elsevier BV
- Published
- 2014-10
- Volume
- 144
- Pages
- 313-323
- ISSN
- 0960-0760 (ISSN-L)
- OpenAlex
- W2088934282
- Cited by
- 15
- References
- 58
- Citations by year
- 2025: 1 · 2022: 2 · 2021: 1 · 2020: 5 · 2019: 3 · 2017: 1 · 2016: 1 · 2015: 1
- FWCI
- 0.41
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- The Journal of Steroid Biochemistry and Molecular Biology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Key Research and Development Program of China (2012CB114101)
- National Natural Science Foundation of China (31230067)
- Ministry of Education of the People's Republic of China (20120131110025)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Receptor Mechanisms and Signaling
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurobiology and Insect Physiology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
- Keywords
- 20-hydroxyecdysone, non-genomic steroid action, Helicoverpa armigera, intracellular calcium release, extracellular calcium influx, protein kinase C, ultraspiracle, transcriptional regulation, RNAi knockdown
- MeSH terms
- Animals, Calcium, Cell Line, Cell Membrane, Ecdysterone, Lepidoptera, Metamorphosis, Biological, Phosphorylation, Protein Kinase C, Signal Transduction, Cyclin-Dependent Kinases, Insect Proteins, RNA Interference, GTP-Binding Protein alpha Subunits, Gq-G11, Ubiquitin-Specific Proteases
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