Cited by 15Receptor Mechanisms and SignalingNeurobiology and Insect Physiology ResearchProtein Kinase Regulation and GTPase Signaling

G-protein αq participates in the steroid hormone 20-hydroxyecdysone nongenomic signal transduction

Ren, Jing, Li, Xiang-Ru, Liu, Peng-Cheng, Cai, Mei-Juan, Liu, Wen, Wang, Jin-Xing, Zhao, Xiao-Fan·2014·The Journal of Steroid Biochemistry and Molecular Biology

Abstract

The nuclear receptor-mediated genomic pathways of the animal steroid hormones are well known. However, the cell membrane receptor-mediated nongenomic pathways of the animal steroid hormones are little understood. In this study, we report the participation of a G-protein alpha q (Gαq)(1) subunit in the 20E nongenomic pathway in the cell membrane and regulating gene expression during molting and metamorphosis in a lepidopteran insect, Helicoverpa armigera. 20E-induced phosphorylation of Gαq was detected using two-dimensional electrophoresis techniques. Knockdown of Gαq by injecting double-stranded RNA suppressed the development of larvae, delayed metamorphosis, and inhibited 20E-induced gene expression. Gαq was distributed throughout the cell, and migrated toward the plasma membrane upon 20E induction. Gαq was necessary in the 20E-induced intracellular Ca(2+) release and extracellular Ca(2+) influx. The protein kinase C (PKC) inhibitor could repress 20E-induced phosphorylation of cyclin-dependent kinase 10 (CDK10) and transcription factor ultraspiracle (USP1). PKC inhibitor could repress the Gαq phosphorylation and membrane trafficking. These results suggest that Gαq participates in 20E signaling in the cell membrane at the pre-genomic stage by modulating the increase of the intracellular Ca(2+) and phosphorylation of CDK10 and USP1 in 20E transcription complex to regulate gene transcription.

Integrity signals

Registry

Crossref · 10.1016/j.jsbmb.2014.08.006

Cites

56 of 56 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Cites

Flagged first, then by citations

2 of the 58 references couldn’t be matched to a known paper.

Cited by

Sorted by citations

Authors
Ren, J., Li, X.-R., Liu, P.-C., Cai, M.-J., Liu, W., Wang, J.-X., & Zhao, X.-F.
Affiliations
Shandong University (CN)
Corresponding author
Zhao, Xiao-Fan
Venue
The Journal of Steroid Biochemistry and Molecular Biology
Publisher
Elsevier BV
Published
2014-10
Volume
144
Pages
313-323
ISSN
0960-0760 (ISSN-L)
OpenAlex
W2088934282
Cited by
15
References
58
Citations by year
2025: 1 · 2022: 2 · 2021: 1 · 2020: 5 · 2019: 3 · 2017: 1 · 2016: 1 · 2015: 1
FWCI
0.41
Type
journal article
Language
en
Open access
closed
Locations
  • The Journal of Steroid Biochemistry and Molecular Biology (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Key Research and Development Program of China (2012CB114101)
  • National Natural Science Foundation of China (31230067)
  • Ministry of Education of the People's Republic of China (20120131110025)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Receptor Mechanisms and Signaling
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurobiology and Insect Physiology Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
Keywords
20-hydroxyecdysone, non-genomic steroid action, Helicoverpa armigera, intracellular calcium release, extracellular calcium influx, protein kinase C, ultraspiracle, transcriptional regulation, RNAi knockdown
MeSH terms
Animals, Calcium, Cell Line, Cell Membrane, Ecdysterone, Lepidoptera, Metamorphosis, Biological, Phosphorylation, Protein Kinase C, Signal Transduction, Cyclin-Dependent Kinases, Insect Proteins, RNA Interference, GTP-Binding Protein alpha Subunits, Gq-G11, Ubiquitin-Specific Proteases

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review