Cited by 39Heat shock proteins researchNeurobiology and Insect Physiology ResearchGenomics and Chromatin Dynamics

In a Nongenomic Action, Steroid Hormone 20-Hydroxyecdysone Induces Phosphorylation of Cyclin-Dependent Kinase 10 to Promote Gene Transcription

Liu, Wen, Cai, Mei-Juan, Wang, Jin-Xing, Zhao, Xiao-Fan·2014·Endocrinology

Abstract

The insect steroid hormone 20-hydroxyecdysone (20E) regulates gene transcription via a genomic pathway by forming a transcription complex that binds to DNA with the help of the chaperone proteins, heat shock proteins (Hsps) Hsc70 and Hsp90. However, the nongenomic mechanisms by which 20E regulates gene expression remain unclear. In this study, we found that 20E regulated the phosphorylation of serine/threonine protein kinase cyclin-dependent kinase 10 (CDK10) through a nongenomic pathway to mediate gene transcription in the lepidopteran Helicoverpa armigera. The down-regulation of CDK10 by RNA interference in larvae and the epidermal cell line delayed development and suppressed 20E-induced gene transcription. CDK10 was localized to the nucleus via its KKRR motif, and this nuclear localization and the ATPase motif were necessary for the efficient expression of the 20E-inducible gene. The rapid phosphorylation of CDK10 was induced by 20E, whereas it was repressed by the inhibitors of G-protein–coupled receptors, phospholipase C, and Ca2+ channels. Phosphorylated CDK10 exhibited increased interactions with Hsps Hsc70 and Hsp90 and then promoted the interactions between Hsps and ecdysone receptor EcRB1 and the binding of the Hsps-EcRB1 complex to the 20E response element for the regulation of gene transcription. CDK10 depletion suppressed the formation of the Hsps-EcRB1 complex at the hormone receptor 3 promoter. These results suggest that 20E induces CDK10 phosphorylation via a nongenomic pathway to regulate gene transcription in the nucleus.

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Crossref · 10.1210/en.2013-2020

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Authors
Liu, W., Cai, M.-J., Wang, J.-X., & Zhao, X.-F.
Affiliations
  • Shandong University (CN)
  • The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Science, Shandong University, Jinan 250100, China
Venue
Endocrinology
Published
2014-05-01
Volume
155
Issue
5
Pages
1738-1750
ISSN
  • 0013-7227 (ISSN-L)
  • 1945-7170
OpenAlex
W1969355291
Cited by
38
References
48
Citations by year
2025: 4 · 2024: 2 · 2023: 4 · 2022: 4 · 2021: 4 · 2020: 4 · 2019: 2 · 2018: 1 · 2017: 2 · 2016: 2 · 2015: 4 · 2014: 4 · 2012: 1
FWCI
1.64
Type
journal article
Language
en
Open access
closed
Locations
  • Endocrinology (publishedVersion)
  • PubMed (publishedVersion)
Funders
Akademie Věd České Republiky
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Heat shock proteins research
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurobiology and Insect Physiology Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
Keywords
20-hydroxyecdysone, non-genomic steroid action, gene transcription, Hsc70, HSP90, heat shock proteins, Helicoverpa armigera, G protein-coupled receptor signaling, phospholipase C, calcium channels, nuclear localization, insect development
MeSH terms
Animals, Cell Line, Cell Nucleus, Ecdysterone, Larva, Lepidoptera, Metamorphosis, Biological, Phosphorylation, Promoter Regions, Genetic, Protein Processing, Post-Translational, Receptors, Steroid, Transcription, Genetic, HSP90 Heat-Shock Proteins, Cyclin-Dependent Kinases, Insect Proteins, Response Elements, Protein Transport, RNA Interference, Epigenesis, Genetic, Membrane Transport Modulators, HSC70 Heat-Shock Proteins, Protein Interaction Domains and Motifs

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