Nonviral gene editing via CRISPR/Cas9 delivery by membrane-disruptive and endosomolytic helical polypeptide
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Effective and safe delivery of the CRISPR/Cas9 gene-editing elements remains a challenge. Here we report the development of PEGylated nanoparticles (named P-HNPs) based on the cationic α-helical polypeptide poly(γ-4-((2-(piperidin-1-yl)ethyl)aminomethyl)benzyl-l-glutamate) for the delivery of Cas9 expression plasmid and sgRNA to various cell types and gene-editing scenarios. The cell-penetrating α-helical polypeptide enhanced cellular uptake and promoted escape of pCas9 and/or sgRNA from the endosome and transport into the nucleus. The colloidally stable P-HNPs achieved a Cas9 transfection efficiency up to 60% and sgRNA uptake efficiency of 67.4%, representing an improvement over existing polycation-based gene delivery systems. After performing single or multiplex gene editing with an efficiency up to 47.3% in vitro, we demonstrated that P-HNPs delivering Cas9 plasmid/sgRNA targeting the polo-like kinase 1 (Plk1) gene achieved 35% gene deletion in HeLa tumor tissue to reduce the Plk1 protein level by 66.7%, thereby suppressing the tumor growth by >71% and prolonging the animal survival rate to 60% within 60 days. Capable of delivering Cas9 plasmids to various cell types to achieve multiplex gene knock-out, gene knock-in, and gene activation in vitro and in vivo, the P-HNP system offers a versatile gene-editing platform for biological research and therapeutic applications.
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A membrane-penetrating cationic α-helical polypeptide enhanced cellular internalization of Cas9 plasmid and sgRNA.
“Similarly, Wang et al. synthesized a membrane-penetrating cationic α-helical polypeptide which enhanced cellular internalization of Cas9 plasmid and sgRNA.[228]”
From RNA delivery biomaterials for the treatment of genetic and rare diseases (2019) · cites this paper as [228]
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Development and Applications of CRISPR-Cas9 for Genome Engineering
Hsu, P. D., Lander, E. S., & Zhang, F. (2014) · Cell · doi:10.1016/j.cell.2014.05.010 · Cited by 5,762 · see citing works
Non-viral vectors for gene-based therapy
Yin, H., Kanasty, R. L., Eltoukhy, A. A., Vegas, A. J., Dorkin, J. R., & Anderson, D. G. (2014) · Nature Reviews Genetics · doi:10.1038/nrg3763 · Cited by 3,123 · see citing works
Zuris, J. A., Thompson, D. B., Shu, Y., Guilinger, J. P., Bessen, J. L., Hu, J. H., Maeder, M. L., Joung, J. K., Chen, Z.-Y., & Liu, D. R. (2015) · Nature Biotechnology · doi:10.1038/nbt.3081 · Cited by 1,460 · see citing works
Therapeutic genome editing: prospects and challenges
Cox, D. B. T., Platt, R. J., & Zhang, F. (2015) · Nature Medicine · doi:10.1038/nm.3793 · Cited by 1,336 · see citing works
RNA-guided gene activation by CRISPR-Cas9-based transcription factors
Perez-Pinera, P., Kocak, D. D., Vockley, C. M., Adler, A. F., Kabadi, A. M., Polstein, L. R., Thakore, P. I., Glass, K. A., Ousterout, D. G., Leong, K. W., et al. (2013) · Nature Methods · doi:10.1038/nmeth.2600 · Cited by 1,328 · see citing works
Self-citationIversen, T., Skotland, T., & Sandvig, K. (2011) · Nano Today · doi:10.1016/j.nantod.2011.02.003 · Cited by 1,235 · see citing works
Identification of preexisting adaptive immunity to Cas9 proteins in humans
Charlesworth, C. T., Deshpande, P. S., Dever, D. P., Camarena, J., Lemgart, V. T., Cromer, M. K., Vakulskas, C. A., Collingwood, M. A., Zhang, L., Bode, N. M., et al. (2019) · Nature Medicine · doi:10.1038/s41591-018-0326-x · Cited by 1,093 · see citing works
Effect of genome size on AAV vector packaging
Wu, Z., Yang, H., & Colosi, P. (2010) · Molecular Therapy · doi:10.1038/mt.2009.255 · Cited by 1,025 · see citing works
Yin, H., Song, C.-Q., Dorkin, J. R., Zhu, L. J., Li, Y., Wu, Q., Park, A., Yang, J., Suresh, S., Bizhanova, A., et al. (2016) · Nature Biotechnology · doi:10.1038/nbt.3471 · Cited by 876 · see citing works
Rapid and highly efficient mammalian cell engineering via Cas9 protein transfection
Liang, X., Potter, J., Kumar, S., Zou, Y., Quintanilla, R., Sridharan, M., Carte, J., Chen, W., Roark, N., Ranganathan, S., et al. (2015) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2015.04.024 · Cited by 733 · see citing works
Gene disruption by cell-penetrating peptide-mediated delivery of Cas9 protein and guide RNA
Ramakrishna, S., Kwaku Dad, A.-B., Beloor, J., Gopalappa, R., Lee, S.-K., & Kim, H. (2014) · Genome Research · doi:10.1101/gr.171264.113 · Cited by 655 · see citing works
Self‐assembled DNA nanoclews for the efficient delivery of CRISPR–Cas9 for genome editing
Sun, W., Ji, W., Hall, J. M., Hu, Q., Wang, C., Beisel, C. L., & Gu, Z. (2015) · Angewandte Chemie International Edition · doi:10.1002/anie.201506030 · Cited by 629 · see citing works
Efficient delivery of genome-editing proteins using bioreducible lipid nanoparticles
Wang, M., Zuris, J. A., Meng, F., Rees, H., Sun, S., Deng, P., Han, Y., Gao, X., Pouli, D., Wu, Q., et al. (2016) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1520244113 · Cited by 624 · see citing works
Direct cytosolic delivery of CRISPR/Cas9-ribonucleoprotein for efficient gene editing
Mout, R., Ray, M., Yesilbag Tonga, G., Lee, Y.-W., Tay, T., Sasaki, K., & Rotello, V. M. (2017) · ACS Nano · doi:10.1021/acsnano.6b07600 · Cited by 579 · see citing works
Wang, H.-X., Li, M., Lee, C. M., Chakraborty, S., Kim, H.-W., Bao, G., & Leong, K. W. (2017) · Chemical Reviews · doi:10.1021/acs.chemrev.6b00799 · Cited by 560 · see citing works
Self-citationMiller, J. B., Zhang, S., Kos, P., Xiong, H., Zhou, K., Perelman, S. S., Zhu, H., & Siegwart, D. J. (2017) · Angewandte Chemie International Edition · doi:10.1002/anie.201610209 · Cited by 540 · see citing works
Gene Therapy Progress and Prospects: Electroporation and other physical methods
Wells, D. J. (2004) · Gene Therapy · doi:10.1038/sj.gt.3302337 · Cited by 373 · see citing works
Polo-like kinases and oncogenesis
Eckerdt, F., Yuan, J., & Strebhardt, K. (2005) · Oncogene · doi:10.1038/sj.onc.1208273 · Cited by 363 · see citing works
Gibbs, R. A., Nguyen, P. N., McBride, L. J., Koepf, S. M., & Caskey, C. T. (1989) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.86.6.1919 · Cited by 275 · see citing works
Exogenous siRNA delivery using peptide transduction domains/cell penetrating peptides
Meade, B. R., & Dowdy, S. F. (2007) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2007.03.004 · Cited by 274 · see citing works
Ionic polypeptides with unusual helical stability
Lu, H., Wang, J., Bai, Y., Lang, J. W., Liu, S., Lin, Y., & Cheng, J. (2011) · Nature Communications · doi:10.1038/ncomms1209 · Cited by 273 · see citing works
Self-citationArtificial Virus Delivers CRISPR-Cas9 System for Genome Editing of Cells in Mice
Li, L., Song, L., Liu, X., Yang, X., Li, X., He, T., Wang, N., Yang, S., Yu, C., Yin, T., et al. (2017) · ACS Nano · doi:10.1021/acsnano.6b04261 · Cited by 244 · see citing works
Smith-Arica, J. R., Maguire, S. F., Davis, L. A., Alexander, M., Yang, F., Chandran, S., ffrench‐Constant, C., & Pedersen, R. A. (2007) · Stem Cells · doi:10.1634/stemcells.2007-0039 · Cited by 241 · see citing works
Macrophage-Specific in Vivo Gene Editing Using Cationic Lipid-Assisted Polymeric Nanoparticles
Luo, Y., Xu, C., Li, H., Cao, Z., Liu, J., Wang, J., Du, X., Yang, X., Gu, Z., & Wang, J. (2018) · ACS Nano · doi:10.1021/acsnano.7b07874 · Cited by 223 · see citing works
Wang, P., Zhang, L., Xie, Y., Wang, N., Tang, R., Zheng, W., & Jiang, X. (2017) · Advanced Science · doi:10.1002/advs.201700175 · Cited by 213 · see citing works
A CRISPR/Cas9-Based System for Reprogramming Cell Lineage Specification
Chakraborty, S., Ji, H., Kabadi, A. M., Gersbach, C. A., Christoforou, N., & Leong, K. W. (2014) · Stem Cell Reports · doi:10.1016/j.stemcr.2014.09.013 · Cited by 197 · see citing works
Self-citationComparative transfection of DNA into primary and transformed mammalian cells from different lineages
Maurisse, R., de Semir, D., Emamekhoo, H., Bedayat, B., Abdolmohammadi, A., Parsi, H., & Gruenert, D. C. (2010) · BMC Biotechnology · doi:10.1186/1472-6750-10-9 · Cited by 190 · see citing works
Komin, A., Russell, L. M., Hristova, K. A., & Searson, P. C. (2016) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2016.06.002 · Cited by 189 · see citing works
Identification of Pre-Existing Adaptive Immunity to Cas9 Proteins in HumansPreprint
Charlesworth, C. T., Deshpande, P. S., Dever, D. P., et al. (2018) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/243345 · Cited by 185 · see citing works
Reactive and Bioactive Cationic α‐Helical Polypeptide Template for Nonviral Gene Delivery
Gabrielson, N. P., Lu, H., Yin, L., Li, D., Wang, F., & Cheng, J. (2011) · Angewandte Chemie International Edition · doi:10.1002/anie.201104262 · Cited by 180 · see citing works
Self-citationYin, L., Song, Z., Kim, K. H., Zheng, N., Gabrielson, N. P., & Cheng, J. (2013) · Advanced Materials · doi:10.1002/adma.201205088 · Cited by 123 · see citing works
Self-citationHe, H., Zheng, N., Song, Z., Kim, K. H., Yao, C., Zhang, R., Zhang, C., Huang, Y., Uckun, F. M., Cheng, J., et al. (2016) · ACS Nano · doi:10.1021/acsnano.5b05470 · Cited by 121 · see citing works
Self-citationYin, L., Song, Z., Qu, Q., Kim, K. H., Zheng, N., Yao, C., Chaudhury, I., Tang, H., Gabrielson, N. P., Uckun, F. M., et al. (2013) · Angewandte Chemie International Edition · doi:10.1002/anie.201209991 · Cited by 92 · see citing works
Self-citationZheng, N., Yin, L., Song, Z., Ma, L., Tang, H., Gabrielson, N. P., Lu, H., & Cheng, J. (2013) · Biomaterials · doi:10.1016/j.biomaterials.2013.09.090 · Cited by 56 · see citing works
Self-citationZheng, N., Song, Z., Yang, J., Liu, Y., Li, F., Cheng, J., & Yin, L. (2017) · Acta Biomaterialia · doi:10.1016/j.actbio.2017.05.001 · Cited by 30 · see citing works
Self-citation
Flagged first, then by citations
Cited by
Sorted by citations
Li, H., Yang, Y., Hong, W., Huang, M., Wu, M., & Zhao, X. (2020) · Signal Transduction and Targeted Therapy · doi:10.1038/s41392-019-0089-y · Cited by 1,771 · see citing works
CRISPR technology: A decade of genome editing is only the beginning
Wang, J. Y., & Doudna, J. A. (2023) · Science · doi:10.1126/science.add8643 · Cited by 1,058 · see citing works
Recent progress in drug delivery
Li, C., Wang, J., Wang, Y., Gao, H., Wei, G., Huang, Y., Yu, H., Gan, Y., Wang, Y., Mei, L., et al. (2019) · Acta Pharmaceutica Sinica B · doi:10.1016/j.apsb.2019.08.003 · Cited by 818 · see citing works
Improving cancer immunotherapy using nanomedicines: progress, opportunities and challenges
Martin, J. D., Cabral, H., Stylianopoulos, T., & Jain, R. K. (2020) · Nature Reviews Clinical Oncology · doi:10.1038/s41571-019-0308-z · Cited by 688 · see citing works
Tissue Engineering and Regenerative Medicine: Achievements, Future, and Sustainability in Asia
Han, F., Wang, J., Ding, L., Hu, Y., Li, W., Yuan, Z., Guo, Q., Zhu, C., Yu, L., Wang, H., et al. (2020) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2020.00083 · Cited by 369 · see citing works
Self-citationMazo, A. R., Allison‐Logan, S., Karimi, F., Chan, N. J.-A., Qiu, W., Duan, W., O’brien-simpson, N. M., & Qiao, G. G. (2020) · Chemical Society Reviews · doi:10.1039/c9cs00738e · Cited by 334 · see citing works
Nanotechnology-based delivery of CRISPR/Cas9 for cancer treatment
Xu, X., Liu, C., Wang, Y., Koivisto, O., Zhou, J., Shu, Y., & Zhang, H. (2021) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2021.113891 · Cited by 295 · see citing works
Self-citationCell-Type-Specific CRISPR/Cas9 Delivery by Biomimetic Metal Organic Frameworks
Alyami, M., Alsaiari, S. K., Li, Y., Qutub, S., Aleisa, F. A., Sougrat, R., Merzaban, J. S., & Khashab, N. M. (2020) · Journal of the American Chemical Society · doi:10.1021/jacs.9b11638 · Cited by 228 · see citing works
Zou, Y., Sun, X., Yang, Q., Zheng, M., Shimoni, O., Ruan, W., Wang, Y., Zhang, D., Yin, J., Huang, X., et al. (2022) · Science Advances · doi:10.1126/sciadv.abm8011 · Cited by 227 · see citing works
Self-citationIn vivo delivery of CRISPR-Cas9 therapeutics: Progress and challenges
Behr, M. P., Zhou, J., Xu, B., & Zhang, H. (2021) · Acta Pharmaceutica Sinica B · doi:10.1016/j.apsb.2021.05.020 · Cited by 220 · see citing works
Delivery of CRISPR/Cas systems for cancer gene therapy and immunotherapy
Song, X., Liu, C., Wang, N., Huang, H., He, S., Gong, C., & Wei, Y. (2020) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2020.04.010 · Cited by 214 · see citing works
Targeted delivery of CRISPR-Cas9 and transgenes enables complex immune cell engineering
Hamilton, J. R., Tsuchida, C. A., Nguyen, D. N., Shy, B. R., McGarrigle, E. R., Espinoza, C. R. S., Carr, D., Blaeschke, F., Marson, A., & Doudna, J. A. (2021) · Cell Reports · doi:10.1016/j.celrep.2021.109207 · Cited by 208 · see citing works
Engineered materials for in vivo delivery of genome-editing machinery
Tong, S., Moyo, B., Lee, C. M., Leong, K. W., & Bao, G. (2019) · Nature Reviews Materials · doi:10.1038/s41578-019-0145-9 · Cited by 206 · see citing works
Self-citationGu, W., Meng, F., Haag, R., & Zhong, Z. (2020) · Journal of Controlled Release · doi:10.1016/j.jconrel.2020.10.003 · Cited by 195 · see citing works
Helical polymers for biological and medical applications
Leigh, T., & Fernández‐Trillo, F. (2020) · Nature Reviews Chemistry · doi:10.1038/s41570-020-0180-5 · Cited by 196 · see citing works
Rational designs of in vivo CRISPR-Cas delivery systems
Xu, C., Chen, G., Luo, Y., Zhang, Y., Zhao, G., Lu, Z.-D., Czarna, A., Gu, Z., & Wang, J. (2019) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2019.11.005 · Cited by 185 · see citing works
Drug delivery systems for CRISPR-based genome editors
Madigan, V. J., Zhang, F., & Dahlman, J. E. (2023) · Nature Reviews Drug Discovery · doi:10.1038/s41573-023-00762-x · Cited by 180 · see citing works
Deng, S., Li, M., Liu, S., Chen, J., Li, M., Chew, S. Y., Leong, K. W., & Cheng, D. (2020) · Science Advances · doi:10.1126/sciadv.abb4005 · Cited by 169 · see citing works
Self-citationCell Penetrating Peptides, Novel Vectors for Gene Therapy
Taylor, R. E., & Zahid, M. (2020) · Pharmaceutics · doi:10.3390/pharmaceutics12030225 · Cited by 160 · see citing works
Song, Z., Fu, H., Wang, R., Pacheco, L. A., Wang, X., Lin, Y., & Cheng, J. (2018) · Chemical Society Reviews · doi:10.1039/c8cs00095f · Cited by 152 · see citing works
Self-citationLi, L., Yáng, Z., Zhu, S., He, L., Fan, W., Tang, W., Zou, J., Shen, Z., Zhang, M., Tang, L., et al. (2019) · Advanced Materials · doi:10.1002/adma.201901187 · Cited by 146 · see citing works
Delivery of CRISPR/Cas9 for therapeutic genome editing
Xu, X., Wan, T. Y., Xin, H., Li, D., Pan, H., Wu, J., & Yuan, P. (2019) · The Journal of Gene Medicine · doi:10.1002/jgm.3107 · Cited by 147 · see citing works
Self-citationHypoxia‐Responsive Gene Editing to Reduce Tumor Thermal Tolerance for Mild‐Photothermal Therapy
Li, X., Pan, Y., Chen, C., Gao, Y., Liu, X., Yang, K., Luan, X., Zhou, D., Zeng, F., Han, X., et al. (2021) · Angewandte Chemie International Edition · doi:10.1002/anie.202107036 · Cited by 144 · see citing works
Engineering Nanoparticles for Targeted Delivery of Nucleic Acid Therapeutics in Tumor
Xiao, Y., Shi, K. Y., Qu, Y., Chu, B., & Qian, Z. (2018) · Molecular Therapy — Methods & Clinical Development · doi:10.1016/j.omtm.2018.09.002 · Cited by 142 · see citing works
Non-viral delivery of the CRISPR/Cas system: DNA versus RNA versus RNP
Lin, Y., Wagner, E., & Lächelt, U. (2022) · Biomaterials Science · doi:10.1039/d1bm01658j · Cited by 141 · see citing works
Applications and developments of gene therapy drug delivery systems for genetic diseases
Pan, X., Veroniaina, H., Su, N., Sha, K., Jiang, F., Wu, Z., & Qi, X. (2021) · Asian Journal of Pharmaceutical Sciences · doi:10.1016/j.ajps.2021.05.003 · Cited by 140 · see citing works
Wei, T., Cheng, Q., Farbiak, L., Anderson, D. G., Langer, R., & Siegwart, D. J. (2020) · ACS Nano · doi:10.1021/acsnano.0c04707 · Cited by 137 · see citing works
He, S., Li, C., Zhang, Q., Ding, J., Liang, X., Chen, X., Xiao, H., Chen, X. S., Zhou, D., & Huang, Y. (2018) · ACS Nano · doi:10.1021/acsnano.8b03476 · Cited by 129 · see citing works
Nanoparticle-Based Delivery of CRISPR/Cas9 Genome-Editing Therapeutics
Givens, B. E., Naguib, Y. W., Geary, S. M., Devor, E. J., & Salem, A. K. (2018) · The AAPS Journal · doi:10.1208/s12248-018-0267-9 · Cited by 132 · see citing works
Khoshandam, M., Soltaninejad, H., Mousazadeh, M., Hamidieh, A. A., & Hosseinkhani, S. (2023) · Genes & Diseases · doi:10.1016/j.gendis.2023.02.027 · Cited by 130 · see citing works
CRISPR/Cas9 systems: Delivery technologies and biomedical applications
Du, Y., Liu, Y., Hu, J., Peng, X., & Liu, Z. (2023) · Asian Journal of Pharmaceutical Sciences · doi:10.1016/j.ajps.2023.100854 · Cited by 135 · see citing works
Ma, Y., Duan, L., Sun, J., Gou, S., Chen, F., Liang, Y., Dai, F., & Xiao, B. (2022) · Biomaterials · doi:10.1016/j.biomaterials.2022.121410 · Cited by 121 · see citing works
Wan, T. Y., Niu, D., Wu, C., Xu, F., Church, G. M., & Yuan, P. (2019) · Materials Today · doi:10.1016/j.mattod.2018.12.003 · Cited by 118 · see citing works
Peptide-based gene delivery vectors
Kang, Z., Meng, Q., & Liu, K. (2019) · Journal of Materials Chemistry B · doi:10.1039/c8tb03124j · Cited by 117 · see citing works
Zhou, Y., Liang, Q., Wu, X., Duan, S., Ge, C., Ye, H., Lu, J., Zhu, R., Chen, Y., Meng, F., et al. (2023) · Advanced Materials · doi:10.1002/adma.202210691 · Cited by 115 · see citing works
Self-citationNon-Viral Delivery To Enable Genome Editing
Rui, Y., Wilson, D. R., & Green, J. J. (2018) · Trends in biotechnology · doi:10.1016/j.tibtech.2018.08.010 · Cited by 115 · see citing works
Guo, P., Yang, J., Huang, J., Auguste, D. T., & Moses, M. A. (2019) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1904697116 · Cited by 112 · see citing works
Nichols, C. A., Gibson, W. J., Brown, M. S., Kosmicki, J. A., Busanovich, J. P., Wei, H. Y., Urbanski, L. M., Currimjee, N., Berger, A. C., Gao, G. F., et al. (2020) · Nature Communications · doi:10.1038/s41467-020-16399-y · Cited by 103 · see citing works
Carrier strategies boost the application of CRISPR/Cas system in gene therapy
Xu, Z., Wang, Q., Zhong, H., Jiang, Y., Shi, X., Yuan, B., Yu, N., Zhang, S., Yuan, X., Guo, S., et al. (2022) · Exploration · doi:10.1002/exp.20210081 · Cited by 104 · see citing works
Mashel, T., Tarakanchikova, Y., Muslimov, A., Zyuzin, M., Timin, A., Lepik, K., & Fehse, B. (2020) · Biomaterials · doi:10.1016/j.biomaterials.2020.120282 · Cited by 92 · see citing works
Liu, X., Zhao, Z., Wu, F., Chen, Y., & Yin, L. (2021) · Advanced Materials · doi:10.1002/adma.202108116 · Cited by 98 · see citing works
Self-citationTang, Q., Liu, J., Jiang, Y. Y., Zhang, M., Mao, L., & Wang, M. (2019) · ACS Applied Materials & Interfaces · doi:10.1021/acsami.9b17749 · Cited by 98 · see citing works
Engineering Precision Medicine
Sun, W., Lee, J., Zhang, S., Benyshek, C., Dokmeci, M. R., & Khademhosseini, A. (2018) · Advanced Science · doi:10.1002/advs.201801039 · Cited by 93 · see citing works
Biomaterials as vectors for the delivery of CRISPR–Cas9
Eoh, J. H., & Gu, L. (2019) · Biomaterials Science · doi:10.1039/c8bm01310a · Cited by 91 · see citing works
α-Amino acid N-carboxyanhydride (NCA)-derived synthetic polypeptides for nucleic acids delivery
Liu, Y., & Yin, L. (2020) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2020.12.007 · Cited by 87 · see citing works
Self-citationHou, M., Wu, X., Zhao, Z., Deng, Q., Chen, Y., & Yin, L. (2022) · Acta Biomaterialia · doi:10.1016/j.actbio.2022.02.018 · Cited by 87 · see citing works
Self-citationCRISPR/Cas13a‐Based MicroRNA Detection in Tumor‐Derived Extracellular Vesicles
Hong, J., Son, T., Castro, C. M., & Im, H. (2023) · Advanced Science · doi:10.1002/advs.202301766 · Cited by 87 · see citing works
Dubey, A. K., Gupta, V. K., Kujawska, M., Orive, G., Kim, N., Li, C.-Z., Mishra, Y. K., & Kaushik, A. K. (2022) · Journal of nanostructure in chemistry · doi:10.1007/s40097-022-00472-7 · Cited by 86 · see citing works
Recent advances in the delivery and applications of nonviral CRISPR/Cas9 gene editing
Sinclair, F., Begum, A. A., Dai, C. C., Tóth, I., & Moyle, P. M. (2023) · Drug Delivery and Translational Research · doi:10.1007/s13346-023-01320-z · Cited by 88 · see citing works
RNA delivery biomaterials for the treatment of genetic and rare diseases
Zhao, W., Hou, X., Vick, O. G., & Dong, Y. (2019) · Biomaterials · doi:10.1016/j.biomaterials.2019.119291 · Cited by 82 · see citing works
Optimizing synthetic nucleic acid and protein nanocarriers: The chemical evolution approach
Freitag, F., & Wagner, E. (2020) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2020.03.005 · Cited by 82 · see citing works
Harnessing nanoparticles for the efficient delivery of the CRISPR/Cas9 system
Rahimi, H., Salehiabar, M., Charmi, J., Barsbay, M., Ghaffarlou, M., Razlighi, M. R., Davaran, S., Khalilov, R. I., Sugiyama, M., Nosrati, H., et al. (2020) · Nano Today · doi:10.1016/j.nantod.2020.100895 · Cited by 83 · see citing works
Tao, Y., Yi, K., Hu, H., Shao, D., & Li, M. (2020) · Journal of Materials Chemistry B · doi:10.1039/d0tb01925a · Cited by 78 · see citing works
Self-citationSynthetic multi-layer nanoparticles for CRISPR-Cas9 genome editing
Tang, H., Zhao, X., & Jiang, X. (2020) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2020.03.001 · Cited by 76 · see citing works
Therapy in Rhodopsin-Mediated Autosomal Dominant Retinitis Pigmentosa
Meng, D., Ragi, S. D., & Tsang, S. H. (2020) · Molecular Therapy · doi:10.1016/j.ymthe.2020.08.012 · Cited by 72 · see citing works
Yu, Q., Liu, Y., Yu, B., Hu, Y., Zhang, N., Zheng, Y., Yang, M., & Xu, F. (2020) · Advanced Science · doi:10.1002/advs.202001424 · Cited by 75 · see citing works
Advances in Nanoparticles as Non-Viral Vectors for Efficient Delivery of CRISPR/Cas9
Kim, M., Hwang, Y., Lim, S., Jang, H., & Kim, H. (2024) · Pharmaceutics · doi:10.3390/pharmaceutics16091197 · Cited by 75 · see citing works
Genetic reprogramming for NK cell cancer immunotherapy with CRISPR/Cas9
Afolabi, L. O., Adeshakin, A. O., Sani, M. M., Bi, J., & Wan, X. (2019) · Immunology · doi:10.1111/imm.13094 · Cited by 74 · see citing works
Zeybekler, S. E., Laraib, U., Arshad, R., Sargazi, S., Rahdar, A., Pandey, S., Thakur, V. K., & Diez-Pascual, A. M. (2021) · Nanomaterials · doi:10.3390/nano11113002 · Cited by 71 · see citing works
Jia, W., Wu, Y., Xie, Y., Yu, M., & Chen, Y. (2025) · Advanced Materials · doi:10.1002/adma.202413603 · Cited by 74 · see citing works
Safe CRISPR: Challenges and Possible Solutions
Pineda, M., Lear, A., Collins, J. P., & Kiani, S. (2018) · Trends in biotechnology · doi:10.1016/j.tibtech.2018.09.010 · Cited by 70 · see citing works
Nanotechnology based CRISPR/Cas9 system delivery for genome editing: Progress and prospect
Deng, H., Huang, W., & Zhang, Z. (2019) · Nano Research · doi:10.1007/s12274-019-2465-x · Cited by 69 · see citing works
Geng, J., Xia, X., Teng, L., Wang, L., Chen, L., Guo, X., Belingon, B., Li, J., Feng, X., Li, X., et al. (2021) · Journal of Controlled Release · doi:10.1016/j.jconrel.2021.11.032 · Cited by 69 · see citing works
Self-citationNanoparticles-mediated CRISPR/Cas9 delivery: Recent advances in cancer treatment
Aghamiri, S., Talaei, S., Ghavidel, A. A., Zandsalimi, F., Masoumi, S., Hafshejani, N. H., & Jajarmi, V. (2020) · Journal of Drug Delivery Science and Technology · doi:10.1016/j.jddst.2020.101533 · Cited by 69 · see citing works
Development of EGFR-targeted evodiamine nanoparticles for the treatment of colorectal cancer
Li, C., Cai, G., Song, D., Gao, R., Teng, P., Zhou, L., Ji, Q., Sui, H. X., Cai, J., Li, Q., et al. (2019) · Biomaterials Science · doi:10.1039/c9bm00613c · Cited by 69 · see citing works
RetractedNonviral Gene Therapy for Cancer: A Review
Hidai, C., & Kitano, H. (2018) · Diseases · doi:10.3390/diseases6030057 · Cited by 68 · see citing works
Peeler, D. J., Thai, S. N., Cheng, Y., Horner, P. J., Sellers, D. L., & Pun, S. H. (2018) · Biomaterials · doi:10.1016/j.biomaterials.2018.11.004 · Cited by 67 · see citing works
Tan, Z., Jiang, Y., Ganewatta, M. S., Kumar, R., Keith, A. R., Twaroski, K., Pengo, T., Tolar, J., Lodge, T. P., & Reineke, T. M. (2019) · Macromolecules · doi:10.1021/acs.macromol.9b01645 · Cited by 67 · see citing works
NanoRNP Overcomes Tumor Heterogeneity in Cancer Treatment
Liu, Q., Cai, J., Zheng, Y., Tan, Y., Wang, Y., Zhang, Z., Zheng, C., Zhao, Y., Liu, C., An, Y., et al. (2019) · Nano Letters · doi:10.1021/acs.nanolett.9b02501 · Cited by 67 · see citing works
Uludağ, H., Ubeda, A., & Ansari, A. S. (2019) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2019.00131 · Cited by 66 · see citing works
Engineered Nanomaterials to Potentiate CRISPR/Cas9 Gene Editing for Cancer Therapy
Yi, K., Kong, H., Lao, Y., Li, D., Mintz, R. L., Fang, T., Chen, G., Tao, Y., Li, M., & Ding, J. (2023) · Advanced Materials · doi:10.1002/adma.202300665 · Cited by 65 · see citing works
Self-citationDelivery of CRISPR/Cas9 by Novel Strategies for Gene Therapy
Wang, L., Zheng, W., Liu, S., Li, B. J., & Jiang, X. (2018) · ChemBioChem · doi:10.1002/cbic.201800629 · Cited by 65 · see citing works
Non-viral strategies for delivering genome editing enzymes
Li, J., Røise, J. J., He, M., Das, R., & Murthy, N. (2020) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2020.09.004 · Cited by 63 · see citing works
Current status and prospect of ZIF-based materials for breast cancer treatment
Zeng, Y., Liao, D., Kong, X., Huang, Q., Zhong, M., Liu, J., Nezamzadeh‐Ejhieh, A., Pan, Y. H., & Song, H. (2023) · Colloids and Surfaces B Biointerfaces · doi:10.1016/j.colsurfb.2023.113612 · Cited by 63 · see citing works
Yang, J., Duan, S., Ye, H., Ge, C., Piao, C., Chen, Y., Lee, M., & Yin, L. (2021) · Advanced Functional Materials · doi:10.1002/adfm.202008960 · Cited by 61 · see citing works
Self-citationLi, M., Sun, X., Yin, M., Shen, J., & Yan, S. (2023) · International Journal of Molecular Sciences · doi:10.3390/ijms24065121 · Cited by 62 · see citing works
Self-citationXie, R., Wang, X., Wang, Y., Ye, M., Zhao, Y., Yandell, B. S., & Gong, S. (2022) · Advanced Materials · doi:10.1002/adma.202110618 · Cited by 61 · see citing works
Shen, Y., Cohen, J. L., Nicoloro, S. M. C., Kelly, M., Yenilmez, B., Henriques, F. D. S., Tsagkaraki, E., Edwards, Y. J. K., Hu, X., Friedline, R. H., et al. (2018) · Journal of Biological Chemistry · doi:10.1074/jbc.ra118.004554 · Cited by 61 · see citing works
Zhao, Z., Liu, X., Hou, M., Zhou, R., Wu, F., Yan, J., Li, W., Zheng, Y., Zhong, Q., Chen, Y., et al. (2022) · Advanced Materials · doi:10.1002/adma.202110560 · Cited by 60 · see citing works
Self-citationChou, S., Yang, P., Ban, Q., Yang, Y. P., Wang, M., Chien, C., Chen, S. J., Sun, N., Zhu, Y., Liu, H., et al. (2020) · Advanced Science · doi:10.1002/advs.201903432 · Cited by 60 · see citing works
Tumour suppression by targeted intravenous non-viral CRISPRa using dendritic polymers
Kretzmann, J. A., Evans, C. W., Moses, C., Sorolla, A., Kretzmann, A. L., Wang, E., Ho, D., Hackett, M. J., Dessauvagie, B. F., Smith, N. M., et al. (2019) · Chemical Science · doi:10.1039/c9sc01432b · Cited by 58 · see citing works
Nanobiomaterial vectors for improving gene editing and gene therapy
Hu, S. W., Ding, T., Tang, H., Guo, H., Cui, W., & Shu, Y. (2023) · Materials Today · doi:10.1016/j.mattod.2023.04.011 · Cited by 58 · see citing works
Chien, Y., Hsiao, Y., Chou, S., Lin, T.-Y., Yarmishyn, A. A., Lai, W., Lee, M., Lin, Y., Lin, T.-W., Hwang, D., et al. (2022) · Journal of Nanobiotechnology · doi:10.1186/s12951-022-01717-x · Cited by 58 · see citing works
The power and the promise of CRISPR/Cas9 genome editing for clinical application with gene therapy
Guo, N., Liu, J., Li, W., Ma, Y., & Fu, D. (2021) · Journal of Advanced Research · doi:10.1016/j.jare.2021.11.018 · Cited by 59 · see citing works
Foley, R. A., Sims, R. A., Duggan, E. C., Olmedo, J. K., Ma, R., & Jonas, S. J. (2022) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2022.973326 · Cited by 57 · see citing works
Biological applications of water-soluble polypeptides with ordered secondary structures
Ge, C., Ye, H., Wu, F., Zhu, J., Song, Z., Liu, Y., & Yin, L. (2020) · Journal of Materials Chemistry B · doi:10.1039/d0tb00902d · Cited by 55 · see citing works
Self-citationIn vivo delivery of CRISPR-Cas9 genome editing components for therapeutic applications
Huang, K. L., Zapata, D. A., Tang, Y., Teng, Y., & Li, Y. (2022) · Biomaterials · doi:10.1016/j.biomaterials.2022.121876 · Cited by 55 · see citing works
Intelligent nanotherapeutic strategies for the delivery of CRISPR system
Chen, C., Zhong, W., Du, S., Li, Y., Zeng, Y., Liu, K., Yang, J., Guan, X., & Han, X. (2022) · Acta Pharmaceutica Sinica B · doi:10.1016/j.apsb.2022.12.013 · Cited by 54 · see citing works
Hematopoietic Stem Cell Gene-Addition/Editing Therapy in Sickle Cell Disease
Germino-Watnick, P., Hinds, M. A., Le, A., Chu, R., Liu, X., & Uchida, N. (2022) · Cells · doi:10.3390/cells11111843 · Cited by 38 · see citing works
Recent Advances in CRISPR/Cas9 Delivery Approaches for Therapeutic Gene Editing of Stem Cells
Lotfi, M., Rad, D. M., Mashhadi, S., Ashouri, A., Mojarrad, M., Mozaffari‐Jovin, S., Farrokhi, S., Hashemi, M., Lotfi, M., Warkiani, M. E., et al. (2023) · Stem Cell Reviews and Reports · doi:10.1007/s12015-023-10585-3 · Cited by 54 · see citing works
Allemailem, K. S., Almatroodi, S. A., Almatroudi, A., Alrumaihi, F., Abdulmonem, W. A., Al‐Megrin, W. A. I., Aljamaan, A. N., Rahmani, A. H., & Khan, A. A. (2023) · International Journal of Molecular Sciences · doi:10.3390/ijms24087052 · Cited by 51 · see citing works
Lee, Y., Mout, R., Luther, D. C., Liu, Y., Castellanos-García, L. J., Burnside, A., Ray, M., Tonga, G. Y., Hardie, J. M., Nagaraj, H., et al. (2019) · Advanced Therapeutics · doi:10.1002/adtp.201900041 · Cited by 51 · see citing works
Zhang, X., He, Q., Sun, J., Gong, H., Cao, Y., Duan, L., Yi, S., Ying, B., & Xiao, B. (2022) · Advanced Healthcare Materials · doi:10.1002/adhm.202200255 · Cited by 51 · see citing works
Gene Therapy for Cardiovascular Disease: Basic Research and Clinical Prospects
Cao, G., Xuan, X., Zhang, R., Hu, J. H., & Dong, H. (2021) · Frontiers in Cardiovascular Medicine · doi:10.3389/fcvm.2021.760140 · Cited by 54 · see citing works
Li, Q., Lv, X., Tang, C., & Yin, C. (2022) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2022.119315 · Cited by 50 · see citing works
Progress of delivery methods for CRISPR-Cas9
Yang, W., Yan, J., Zhuang, P., Ding, T., Chen, Y., Zhang, Y., Zhang, H., & Cui, W. (2022) · Expert Opinion on Drug Delivery · doi:10.1080/17425247.2022.2100342 · Cited by 51 · see citing works
Strategies for the CRISPR-Based Therapeutics
Li, B., Niu, Y., Ji, W., & Dong, Y. (2019) · Trends in Pharmacological Sciences · doi:10.1016/j.tips.2019.11.006 · Cited by 49 · see citing works
Enhancing CRISPR/Cas systems with nanotechnology
Chowdhry, R., Lu, S. Z., Lee, S., Godhulayyagari, S., Ebrahimi, S. B., & Samanta, D. (2023) · Trends in biotechnology · doi:10.1016/j.tibtech.2023.06.005 · Cited by 49 · see citing works
Nanovesicle-Mediated Delivery Systems for CRISPR/Cas Genome Editing
Kim, D., Le, Q., Wu, Y., Park, J.-W., & Oh, Y. (2020) · Pharmaceutics · doi:10.3390/pharmaceutics12121233 · Cited by 50 · see citing works
Self-citationYi, K., Kong, H., Zheng, C., Zhuo, C., Jin, Y., Zhong, Q., Mintz, R. L., Ju, E., Wang, H., Lv, S., et al. (2023) · Biomaterials · doi:10.1016/j.biomaterials.2023.122349 · Cited by 47 · see citing works
Self-citation
- Authors
- Wang, H.-X., Song, Z., Lao, Y.-H., Xu, X., Gong, J., Cheng, D., Chakraborty, S., Park, J. S., Li, M., Huang, D., Yin, L., Cheng, J., & Leong, K. W.
- Affiliations
- Department of Biomedical Engineering, Columbia University, New York, NY 10027;
- University of Illinois Urbana-Champaign (US)
- Columbia University (US)
- Soochow University (CN)
- Sun Yat-sen University (CN)
- Corresponding author
- Yin, Lichen
- Cheng, Jianjun
- Leong, Kam W.
- ORCID
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 2018-04-23
- Volume
- 115
- Issue
- 19
- Pages
- 4903-4908
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC5948953
- OpenAlex
- W2802061036
- Cited by
- 277
- References
- 34
- Citations by year
- 2026: 9 · 2025: 18 · 2024: 21 · 2023: 41 · 2022: 47 · 2021: 41 · 2020: 43 · 2019: 42 · 2018: 15
- FWCI
- 12.54
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion, cc-by-nc-nd)
- Europe PMC (PubMed Central) (publishedVersion, cc-by-nc-nd)
- PubMed Central (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- Columbia Academic Commons (Columbia University)
- Funders
- National Natural Science Foundation of China (51573123, 51722305)
- Ministry of Science and Technology of the People's Republic of China (2016YFA0201200)
- Guangdong Innovative and Entrepreneurial Research Team Program (NO.2013S086)
- Global Research Laboratory Program Korean NSF (2015032163)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
- Keywords
- CRISPR/Cas9 delivery, PEGylated nanoparticles, cell-penetrating peptides, endosomal escape, multiplex genome editing, PLK1, tumor growth inhibition, gene knock-in, gene activation
- MeSH terms
- Gene Editing, Animals, HeLa Cells, Humans, Plasmids, Gene Transfer Techniques, K562 Cells, NIH 3T3 Cells, Mice, Nanoparticles, HEK293 Cells, Cell-Penetrating Peptides, CRISPR-Cas Systems
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