Biological reactivity of hypochlorous acid: implications for microbicidal mechanisms of leukocyte myeloperoxidase.

Albrich, J M; McCarthy, C A; Hurst, J K

doi:10.1073/pnas.78.1.210

Cited by 442Neutrophil, Myeloperoxidase and Oxidative MechanismsRedox biology and oxidative stressBioactive Compounds and Antitumor Agents

Biological reactivity of hypochlorous acid: implications for microbicidal mechanisms of leukocyte myeloperoxidase.

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Albrich, J M, McCarthy, C A, Hurst, J K·1981·Proceedings of the National Academy of Sciences

DOI 10.1073/pnas.78.1.210PMC PMC319021

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Abstract

Oxidative degradation of biological substrates by hypochlorous acid has been examined under reaction conditions similar to those found in active phagosomes. Iron sulfur proteins are bleached extremely rapidly, followed in decreasing order by beta-carotene, nucleotides, porphyrins, and heme proteins. Enzymes containing essential cysteine molecules are inactivated with an effectiveness that roughly parallels the nucleophilic reactivities of their sulfhydryl groups. Other compounds, including glucosamines, quinones, riboflavin, and, except for N-chlorination, phospholipids, are unreactive. Rapid irreversible oxidation of cytochromes, adenine nucleotides, and carotene pigments occurs when bacterial cells are exposed to exogenous hypochlorous acid; with Escherichia coli, titrimetric oxidation of cytochrome was found to coincide with loss of aerobic respiration. The occurrence of these cellular reactions implicates hypochlorous acid as a primary microbicide in myeloperoxidase-containing leukocytes; the reactivity patterns observed are consistent with the view that bactericidal action results primarily from loss of energy-linked respiration due to destruction of cellular electron transport chains and the adenine nucleotide pool.

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Authors: Albrich, J. M., McCarthy, C. A., & Hurst, J. K.
ORCID: Hurst, J K
Venue: Proceedings of the National Academy of Sciences
Publisher: National Academy of Sciences
Published: 1981-01
Volume: 78
Issue: 1
Pages: 210-214
ISSN: 0027-8424 (ISSN-L)
1091-6490
DOI: 10.1073/pnas.78.1.210
PMC: PMC319021
OpenAlex: W2158604239
Cited by: 442
References: 38
Citations by year: 2026: 2 · 2025: 5 · 2024: 3 · 2023: 6 · 2022: 10 · 2021: 10 · 2020: 16 · 2019: 10 · 2018: 13 · 2017: 6 · 2016: 6 · 2015: 7 · 2014: 14 · 2013: 15 · 2012: 11
FWCI: 4.21
Type: journal article
Language: en
Open access: green
www.ncbi.nlm.nih.gov
Locations: Proceedings of the National Academy of Sciences (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
Funders: Oregon State University
National Institutes of Health
Topics: Life Sciences › Immunology and Microbiology › Immunology › Neutrophil, Myeloperoxidase and Oxidative Mechanisms
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Toxicology › Bioactive Compounds and Antitumor Agents
Keywords: hypochlorous acid, myeloperoxidase, oxidative degradation, iron-sulfur proteins, cytochrome oxidation, beta-carotene, nucleotide oxidation, heme proteins, Escherichia coli, electron transport chain, bactericidal activity
MeSH terms: Amines, Amino Acids, Amino Sugars, Bacteria, Carotenoids, Cytochromes, Enzyme Inhibitors, Ferredoxins, Hypochlorous Acid, Peroxidase, Nucleotides, Oxidation-Reduction, Peroxidases, Porphyrins, Sulfhydryl Compounds

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Biological reactivity of hypochlorous acid: implications for microbicidal mechanisms of leukocyte myeloperoxidase.

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