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Cited by 155Neuroscience and Neuropharmacology ResearchAmino Acid Enzymes and MetabolismDrug Transport and Resistance Mechanisms

A novel postsynaptic density protein: the monocarboxylate transporter MCT2 is co-localized with δ-glutamate receptors in postsynaptic densities of parallel fiber-Purkinje cell synapses

Bergersen, Linda, Wærhaug, Ola, Helm, Johannes, Thomas, Marion, Laake, Petter, Davies, Andrew J., Wilson, Mariangela C., Halestrap, Andrew P. +1 more·2001·Experimental Brain Research
DOI 10.1007/s002210000600
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Abstract

Confocal immunofluorescence microscopy showed strong monocarboxylate transporter 2 (MCT2) labeling of Purkinje cell bodies and punctate labeling in the molecular layer. By immunogold cytochemistry, it could be demonstrated that the MCT2 immunosignal was concentrated at postsynaptic densities of parallel fiber-Purkinje cell synapses. The distribution of MCT2 transporters within the individual postsynaptic densities mimicked that of the delta2 glutamate receptor, as shown by use of two different gold-particle sizes. The MCT2 distribution was also compared with the distributions of other monocarboxylate transporters (MCT1 and MCT4). The MCT1 immunolabeling was localized in the endothelial cells, while MCT4 immunogold particles were associated with glial profiles, including those abutting the synaptic cleft of the parallel fiber-spine synapses. The postsynaptic density (PSD) molecules identified so far can be divided into five classes: receptors, their anchoring molecules, molecules involved in signal transduction, ion channels, and attachment proteins. Here, we provide evidence that this list of molecules must now be extended to comprise an organic molecule transporter: the monocarboxylate transporter MCT2. The present data suggest that MCT2 has specific transport functions related to the synaptic cleft and that this transporter may allow an influx of lactate derived from perisynaptic glial processes. The expression of MCT2 in synaptic membranes may allow energy supply to be tuned to the excitatory drive.

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  • The absence of mitochondria from dendritic spines makes it questionable whether the role of MCTs is to support astrocyte-to-neuron movement of lactate rather than efflux of lactate produced by aerobic glycolysis in spines.

    “But the absence of mitochondria from dendritic spines does make it questionable whether the role of MCTs, which may be inserted into spine membranes together with AMPA receptors (Bergersen et al., 2001), is to support astrocyte-to-neuron movement of lactate rather than efflux of lactate produced by aerobic glycolysis in the spines.”

    From Fueling thought: Management of glycolysis and oxidative phosphorylation in neuronal metabolism (2018) · cites this paper as Bergersen et al., 2001

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Authors
Bergersen, L., Wærhaug, O., Helm, J., Thomas, M., Laake, P., Davies, A. J., Wilson, M. C., Halestrap, A. P., & Ottersen, O. P.
Affiliations
  • University of Oslo (NO)
  • University of Bristol (GB)
ORCID
Venue
Experimental Brain Research
Publisher
Springer Science and Business Media LLC
Published
2001-02-19
Volume
136
Issue
4
Pages
523-534
ISSN
  • 0014-4819 (ISSN-L)
  • 1432-1106
DOI
10.1007/s002210000600
OpenAlex
W2129323165
Cited by
155
References
47
Citations by year
2026: 3 · 2025: 1 · 2024: 1 · 2023: 2 · 2022: 4 · 2021: 5 · 2020: 4 · 2019: 5 · 2018: 6 · 2017: 8 · 2016: 4 · 2015: 7 · 2014: 9 · 2013: 13 · 2012: 6
FWCI
3.93
Type
journal article
Language
en
Open access
closed
Licence
www.springer.com/tdm (tdm)
Locations
  • Experimental Brain Research (publishedVersion)
  • PubMed (publishedVersion)
  • Bristol Research (University of Bristol) (publishedVersion)
  • Explore Bristol Research (submittedVersion)
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Amino Acid Enzymes and Metabolism
  • Health Sciences › Medicine › Oncology › Drug Transport and Resistance Mechanisms
Keywords
MCT2, postsynaptic density, parallel fiber-Purkinje cell synapse, Purkinje cells, MCT1, MCT4, lactate transport, synaptic cleft, glial processes, cerebellum
MeSH terms
Animals, Antibodies, Carrier Proteins, Male, Purkinje Cells, Synapses, Immunoblotting, Microscopy, Immunoelectron, Rats, Wistar, Receptors, Glutamate, Monocarboxylic Acid Transporters, Rats

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