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A detergent-free method for purifying caveolae membrane from tissue culture cells.
Open accessAbstract
Current methods for purifying caveolae from tissue culture cells take advantage of the Triton X-100 insolubility of this membrane domain. To circumvent the use of detergents, we have developed a method that depends upon the unique buoyant density of caveolae membrane. The caveolae fractions that we obtain are highly enriched in caveolin. As a consequence we are able to identify caveolae-associated proteins that had previously gone undetected. Moreover, resident caveolae proteins that are soluble in Triton X-100 are retained during the isolation.
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Metadata
- Authors
- Smart, E. J., Ying, Y. S., Mineo, C., & Anderson, R. G.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1995-10-24
- Volume
- 92
- Issue
- 22
- Pages
- 10104-10108
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2032190558
- Cited by
- 728
- References
- 1
- FWCI
- 12.85
- Type
- journal article
- Language
- en
- Open access
- green
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