The DOI matches its Crossref record; 40 of the 40 references it cites were found in a registry or an index.
Clustered DNA damages induced in isolated DNA and in human cells by low doses of ionizing radiation
Open accessAbstract
Clustered DNA damages—two or more closely spaced damages (strand breaks, abasic sites, or oxidized bases) on opposing strands—are suspects as critical lesions producing lethal and mutagenic effects of ionizing radiation. However, as a result of the lack of methods for measuring damage clusters induced by ionizing radiation in genomic DNA, neither the frequencies of their production by physiological doses of radiation, nor their repairability, nor their biological effects are known. On the basis of methods that we developed for quantitating damages in large DNAs, we have devised and validated a way of measuring ionizing radiation-induced clustered lesions in genomic DNA, including DNA from human cells. DNA is treated with an endonuclease that induces a single-strand cleavage at an oxidized base or abasic site. If there are two closely spaced damages on opposing strands, such cleavage will reduce the size of the DNA on a nondenaturing gel. We show that ionizing radiation does induce clustered DNA damages containing abasic sites, oxidized purines, or oxidized pyrimidines. Further, the frequency of each of these cluster classes is comparable to that of frank double-strand breaks; among all complex damages induced by ionizing radiation, double-strand breaks are only about 20%, with other clustered damage constituting some 80%. We also show that even low doses (0.1–1 Gy) of high linear energy transfer ionizing radiation induce clustered damages in human cells.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
The Chemical Basis of Radiation Biology
O'Neill, P. (1987) · International Journal of Radiation Biology and Related Studies in Physics, Chemistry and Medicine · doi:10.1080/09553008714552571 · Cited by 1,835
The chemical basis of radiation biology
von Sonntag, C. (1987) · Medical Entomology and Zoology · Cited by 1,760
Initial Events in the Cellular Effects of Ionizing Radiations: Clustered Damage in DNA
Goodhead, D. T. (1994) · International Journal of Radiation Biology · doi:10.1080/09553009414550021 · Cited by 1,204
8-oxoguanine (8-hydroxyguanine) DNA glycosylase and its substrate specificity.
Tchou, J., Kasai, H., Shibutani, S., Chung, M. H., Laval, J., Grollman, A. P., & Nishimura, S. (1991) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.88.11.4690 · Cited by 683
Self-citationThe Complexity of DNA Damage: Relevance to Biological Consequences
Ward, J. F. (1994) · International Journal of Radiation Biology · doi:10.1080/09553009414551401 · Cited by 633
Boiteux, S., Gajewski, E., Laval, J., & Dizdaroglu, M. (1992) · Biochemistry · doi:10.1021/bi00116a016 · Cited by 593
Self-citationTwo Pathways for Base Excision Repair in Mammalian Cells
Frosina, G., Fortini, P., Rossi, O., Carrozzino, F., Raspaglio, G., Cox, L. S., Lane, D. P., Abbondandolo, A., & Dogliotti, E. (1996) · Journal of Biological Chemistry · doi:10.1074/jbc.271.16.9573 · Cited by 534
Ward, J. F. (1985) · Radiation Research · doi:10.2307/3576637 · Cited by 439
Boiteux, S., O'Connor, T. R., Lederer, F., Gouyette, A., & Laval, J. (1990) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)39680-2 · Cited by 348
Self-citationThe Role of DNA Single- and Double-Strand Breaks in Cell Killing by Ionizing Radiation
Olive, P. L. (1998) · Radiation Research · doi:10.2307/3579807 · Cited by 345
Cited by
Evidence for a lack of DNA double-strand break repair in human cells exposed to very low x-ray doses
Rothkamm, K., & Löbrich, M. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0830918100 · Cited by 1,645
Audebert, M., Salles, B., & Calsou, P. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.m404524200 · Cited by 707
Cucinotta, F. A., & Durante, M. (2006) · The Lancet Oncology · doi:10.1016/s1470-2045(06)70695-7 · Cited by 697
Heavy ion carcinogenesis and human space exploration
Durante, M., & Cucinotta, F. A. (2008) · Nature Reviews Cancer · doi:10.1038/nrc2391 · Cited by 578
Mechanisms and Consequences of Double‐Strand DNA Break Formation in Chromatin
Cannan, W. J., & Pederson, D. S. (2016) · Journal of Cellular Physiology · doi:10.1002/jcp.25048 · Cited by 499
Metadata
- Authors
- Sutherland, B. M., Bennett, P. V., Sidorkina, O., & Laval, J.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2000-01-04
- Volume
- 97
- Issue
- 1
- Pages
- 103-108
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2111313313
- Cited by
- 473
- References
- 40
- FWCI
- 21.09
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Clustered DNA damages induced in isolated DNA and in human cells by low doses of ionizing radiation. https://app.cochat.ai/papers/10.1073/pnas.97.1.103
- The paper
- Sutherland, B. M., Bennett, P. V., Sidorkina, O., & Laval, J. (2000). Clustered DNA damages induced in isolated DNA and in human cells by low doses of ionizing radiation. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.97.1.103
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
