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Regulation and immunolocalization of acyl-coenzyme A: cholesterol acyltransferase in mammalian cells as studied with specific antibodies
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Acyl-coenzyme A:cholesterol acyltransferase (ACAT) catalyzes the formation of intracellular cholesterol esters in various tissues. We recently reported the cloning and expression of human macrophage ACAT cDNA. In the current study, we report the production of specific polyclonal antibodies against ACAT by immunizing rabbits with the recombinant fusion protein composed of glutathione S-transferase and the first 131 amino acids of ACAT protein. Immunoblot analysis showed that the antibodies cross-reacted with a 50-kDa protein band from a variety of human cell lines. These antibodies immunodepleted more than 90% of detergent-solubilized ACAT activities from six different human cell types, demonstrating that the 50-kDa protein is the major ACAT catalytic component in these cells. In multiple human tissues examined, the antibodies recognized protein bands with various molecular weights. These antibodies also cross-reacted with the ACAT protein in Chinese hamster ovary cells. Immunoblot analysis showed that the ACAT protein contents in human fibroblast cells, HepG2 cells, or Chinese hamster ovary cells were not affected by sterol in the medium, demonstrating that the main mechanism for sterol-dependent regulation of ACAT activity in these cells is not change in ACAT protein content. As revealed by indirect immunofluorescent microscopy, the ACAT protein in tissue culture cells was located in the endoplasmic reticulum. This finding, along with earlier studies, suggests that cholesterol concentration in the endoplasmic reticulum may be the major determinant for regulating ACAT activity in the intact cells.
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Cites
Molecular Cloning: A Laboratory Manual
Sambrook, J., Fritsch, E., & Maniatis, T. (1989) · UCLA - Biblioteca de Ciencias de la Salud · Cited by 133,589
Molecular cloning: A laboratory manual
Deininger, P. L. (1990) · Analytical Biochemistry · doi:10.1016/0003-2697(90)90595-z · Cited by 84,684
Antibodies, a laboratory manual
(1989) · Biomedicine & Pharmacotherapy · doi:10.1016/0753-3322(89)90130-3 · Cited by 12,318
Regulation of the mevalonate pathway
Goldstein, J. L., & Brown, M. S. (1990) · Nature · doi:10.1038/343425a0 · Cited by 5,394
The Low-Density Lipoprotein Pathway and its Relation to Atherosclerosis
Goldstein, L. J., & Brown, S. M. (1977) · Annual Review of Biochemistry · doi:10.1146/annurev.bi.46.070177.004341 · Cited by 1,906
Chang, C. C., Huh, H. Y., Cadigan, K. M., & Chang, T. (1993) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)36846-2 · Cited by 389
Self-citationMembrane-bound domain of HMG CoA reductase is required for sterol-enhanced degradation of the enzyme
Gil, G., Faust, J. R., Chin, D. J., Goldstein, J. L., & Brown, M. S. (1985) · Cell · doi:10.1016/0092-8674(85)90078-9 · Cited by 386
Localization of endoplasmic reticulum in living and glutaraldehyde-fixed cells with fluorescent dyes
Terasaki, M., Song, J., Wong, J. R., Weiss, M. J., & Chen, L. B. (1984) · Cell · doi:10.1016/0092-8674(84)90530-0 · Cited by 385
Cholesterol ester formation in cultured human fibroblasts. Stimulation by oxygenated sterols.
Brown, M., Dana, S., & Goldstein, J. (1975) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)41498-1 · Cited by 364
Hep G2 cells as a resource for metabolic studies: lipoprotein, cholesterol, and bile acids
Javitt, N. B. (1990) · The FASEB Journal · doi:10.1096/fasebj.4.2.2153592 · Cited by 357
Research works printed but not linked: 1
- Uelmen, 1995, J. Biol. Chem.
Cited by
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Oxysterols: Modulators of Cholesterol Metabolism and Other Processes
Schroepfer, G. J. (2000) · Physiological Reviews · doi:10.1152/physrev.2000.80.1.361 · Cited by 984
The Surface of Lipid Droplets Is a Phospholipid Monolayer with a Unique Fatty Acid Composition
Tauchi-Sato, K., Ozeki, S., Houjou, T., Taguchi, R., & Fujimoto, T. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.m207712200 · Cited by 753
Mechanisms of lipid-body formation
Murphy, D. J., & Vance, J. E. (1999) · Trends in Biochemical Sciences · doi:10.1016/s0968-0004(98)01349-8 · Cited by 577
ACYL-COENZYME A:CHOLESTEROL ACYLTRANSFERASE
Chang, T. Y., Chang, C. C. Y., & Cheng, D. (1997) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.66.1.613 · Cited by 440
Self-citationAcyl‐coenzyme A:cholesterol acyltransferases
Chang, T.-Y., Li, B.-L., Chang, C. C. Y., & Urano, Y. (2009) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.90926.2008 · Cited by 443
Self-citationPerilipin A Increases Triacylglycerol Storage by Decreasing the Rate of Triacylglycerol Hydrolysis
Brasaemle, D., Rubin, B., Harten, I. A., Gruia-Gray, J., Kimmel, A. R., & Londos, C. (2000) · Journal of Biological Chemistry · doi:10.1074/jbc.m007322200 · Cited by 438
Oelkers, P. M., Behari, A., Cromley, D. A., Billheimer, J. T., & Sturley, S. L. (1998) · Journal of Biological Chemistry · doi:10.1074/jbc.273.41.26765 · Cited by 377
Imaging of Lipid Biosynthesis: How a Neutral Lipid Enters Lipid Droplets
Kuerschner, L., Moessinger, C., & Thiele, C. (2008) · Traffic · doi:10.1111/j.1600-0854.2007.00689.x · Cited by 411
Three pools of plasma membrane cholesterol and their relation to cholesterol homeostasis
Das, A., Brown, M. S., Anderson, D. D., Goldstein, J. L., & Radhakrishnan, A. (2014) · eLife · doi:10.7554/elife.02882 · Cited by 354
Anderson, R. A., Joyce, C. W., Davis, M., Reagan, J. W., Clark, M. A., Shelness, G. S., & Rudel, L. L. (1998) · Journal of Biological Chemistry · doi:10.1074/jbc.273.41.26747 · Cited by 302
Vesicular and non-vesicular sterol transport in living cells
Hao, M., Lin, S. X., Karylowski, O. J., Wüstner, D., McGraw, T. E., & Maxfield, F. R. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.m108861200 · Cited by 285
Smet, E. D., Mensink, R. P., & Plat, J. (2012) · Molecular Nutrition & Food Research · doi:10.1002/mnfr.201100722 · Cited by 281
Sterol Esterification in Yeast: A Two-Gene Process
Yang, H., Bard, M., Bruner, D. A., Gleeson, A. M., Deckelbaum, R. J., Aljinovic, G., Pohl, T., Rothstein, R. J., & Sturley, S. L. (1996) · Science · doi:10.1126/science.272.5266.1353 · Cited by 279
Meiner, V., Cases, S., Myers, H. M., Sande, E., Bellosta, S., Schambelan, M., Pitas, R. E., McGuire, J., Herz, J., & Farese, R. V. (1996) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.93.24.14041 · Cited by 261
Kanehara, K., Ito, K., & Akiyama, Y. (2002) · Genes & Development · doi:10.1101/gad.1002302 · Cited by 258
Wilcox, L. J., Borradaile, N. M., de Dreu, L. E., & Huff, M. W. (2001) · Journal of Lipid Research · doi:10.1016/s0022-2275(20)31634-5 · Cited by 254
Roles of acyl-coenzyme A : cholesterol acyltransferase-1 and -2
Chang, T., Chang, C. C. Y., Lin, S., Yu, C., Li, B.-L., & Miyazaki, A. (2001) · Current Opinion in Lipidology · doi:10.1097/00041433-200106000-00008 · Cited by 256
Self-citationIntestinal lipid absorption and transport
Cam, T. P. (2001) · Frontiers in bioscience · doi:10.2741/phan · Cited by 252
Increased atherosclerosis in LDL receptor–null mice lacking ACAT1 in macrophages
Fazio, S., Major, A. S., Swift, L. L., Gleaves, L. A., Accad, M. F., Linton, M. F., & Farese, R. V. (2001) · Journal of Clinical Investigation · doi:10.1172/jci10310 · Cited by 243
Immunological Quantitation and Localization of ACAT-1 and ACAT-2 in Human Liver and Small Intestine
Chang, C. C. Y., Sakashita, N., Ørnvold, K., Lee, O., Chang, E. T., Dong, R., Lin, S., Lee, C. G., Strom, S. C., Kashyap, R., et al. (2000) · Journal of Biological Chemistry · doi:10.1074/jbc.m003927200 · Cited by 228
Self-citationMammalian acyl-CoA:cholesterol acyltransferases
Buhman, K. K. (2000) · Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids · doi:10.1016/s1388-1981(00)00144-x · Cited by 214
Lee, R. G., Willingham, M. C., Davis, M. A., Skinner, K. A., & Rudel, L. L. (2000) · Journal of Lipid Research · doi:10.1016/s0022-2275(20)32360-9 · Cited by 196
Contribution of Are1p and Are2p to steryl ester synthesis in the yeast Saccharomyces cerevisiae
Zweytick, D., Leitner, E., Kohlwein, S. D., Yu, C., Rothblatt, J. A., & Daum, G. (2000) · European Journal of Biochemistry · doi:10.1046/j.1432-1327.2000.01103.x · Cited by 180
Moessinger, C., Kuerschner, L., Spandl, J., Shevchenko, A., & Thiele, C. (2011) · Journal of Biological Chemistry · doi:10.1074/jbc.m110.202424 · Cited by 176
The Enzymes of Neutral Lipid Synthesis
Buhman, K. K., Chen, H. C., & Farese, R. V. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.r100050200 · Cited by 172
Localization and role of NPC1L1 in cholesterol absorption in human intestine
Sané, A. T., Sinnett, D., DELVIN, E. E., Bendayan, M., Marcil, V., Ménard, D., Beaulieu, J., & Levy, E. H. (2006) · Journal of Lipid Research · doi:10.1194/jlr.m600174-jlr200 · Cited by 171
Rogers, M. A., Liu, J., Song, B., Li, B.-L., Chang, C. C. Y., & Chang, T. (2014) · The Journal of Steroid Biochemistry and Molecular Biology · doi:10.1016/j.jsbmb.2014.09.008 · Cited by 167
Self-citationMiyazaki, A., Sakashita, N., Lee, O., Takahashi, K., Horiuchi, S., Hakamata, H., Morganelli, P. M., Chang, C. C. Y., & Chang, T. (1998) · Arteriosclerosis Thrombosis and Vascular Biology · doi:10.1161/01.atv.18.10.1568 · Cited by 151
Self-citationYu, C., Kennedy, N. J., Chang, C. C. Y., & Rothblatt, J. A. (1996) · Journal of Biological Chemistry · doi:10.1074/jbc.271.39.24157 · Cited by 143
Skiba, P. J., Zha, X., Maxfield, F. R., Schissel, S. L., & Tabas, I. A. (1996) · Journal of Biological Chemistry · doi:10.1074/jbc.271.23.13392 · Cited by 135
Chang, C. C. Y., Lee, C. G., Chang, E. T., Cruz, J. C., Levesque, M. C., & Chang, T. (1998) · Journal of Biological Chemistry · doi:10.1074/jbc.273.52.35132 · Cited by 133
Self-citationSakashita, N., Miyazaki, A., Takeya, M., Horiuchi, S., Chang, C. C. Y., Chang, T., & Takahashi, K. (2000) · American Journal Of Pathology · doi:10.1016/s0002-9440(10)64723-2 · Cited by 126
Self-citationEffect of micellar beta-sitosterol on cholesterol metabolism in CaCo-2 cells
Field, F. J., Born, E. J., & Mathur, S. N. (1997) · Journal of Lipid Research · doi:10.1016/s0022-2275(20)37447-2 · Cited by 125
Temel, R. E., Gebre, A. K., Parks, J. S., & Rudel, L. L. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m308235200 · Cited by 118
An examination of the factors affecting intestinal lymphatic transport of dietary lipids
Nordskog, B. K. (2001) · Advanced Drug Delivery Reviews · doi:10.1016/s0169-409x(01)00147-8 · Cited by 116
Lange, Y., & Steck, T. L. (1997) · Journal of Biological Chemistry · doi:10.1074/jbc.272.20.13103 · Cited by 105
Deficiency in ethanolamine plasmalogen leads to altered cholesterol transport
Munn, N. J., Arnio, E., Liu, D., Zoeller, R. A., & Liscum, L. (2003) · Journal of Lipid Research · doi:10.1194/jlr.m200363-jlr200 · Cited by 115
Li, B.-L., Li, X.-L., Duan, Z., Lee, O., Song, L., Ma, Z., Chang, C. C. Y., Yang, X., Park, J. P., Mohandas, T. K., et al. (1999) · Journal of Biological Chemistry · doi:10.1074/jbc.274.16.11060 · Cited by 115
Self-citationCholesterol balance and metabolism in mice with loss of function of Niemann-Pick C protein
Xie, C., Turley, S. D., Pentchev, P. G., & Dietschy, J. M. (1999) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.1999.276.2.e336 · Cited by 115
Multidrug Resistance (MDR1) P-glycoprotein Enhances Esterification of Plasma Membrane Cholesterol
Luker, G. D., Nilsson, K. R., Covey, D. F., & Piwnica-Worms, D. R. (1999) · Journal of Biological Chemistry · doi:10.1074/jbc.274.11.6979 · Cited by 114
Ikenoya, M., Yoshinaka, Y., Kobayashi, H., Kawamine, K., Shibuya, K., Sato, F., Sawanobori, K., Watanabe, T., & Miyazaki, A. (2006) · Atherosclerosis · doi:10.1016/j.atherosclerosis.2006.05.048 · Cited by 113
Intracellular cholesterol trafficking: role of the NPC1 protein
Blanchette‐Mackie, E. J. (2000) · Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids · doi:10.1016/s1388-1981(00)00055-x · Cited by 112
Ku, C. S., Pham, T. X., Park, Y., Kim, B., Shin, M. S., Kang, I., & Lee, J. (2013) · Biochimica et Biophysica Acta (BBA) - General Subjects · doi:10.1016/j.bbagen.2013.01.018 · Cited by 109
Shibuya, Y., Chang, C. C. Y., Huang, L.-H., Bryleva, E. Y., & Chang, T.-Y. (2014) · The Journal of Neuroscience · doi:10.1523/jneurosci.2567-14.2014 · Cited by 108
“Rabbit anti-ACAT1 (DM10) was reported previously (Chang et al., 1995).”
BackgroundSelf-citationACAT1/SOAT1 as a Therapeutic Target for Alzheimer's Disease
Shibuya, Y., Chang, C. C., & Chang, T.-Y. (2015) · Future Medicinal Chemistry · doi:10.4155/fmc.15.161 · Cited by 113
Self-citationRole of ORPs in Sterol Transport from Plasma Membrane to ER and Lipid Droplets in Mammalian Cells
Jansen, M., Ohsaki, Y., Rega, L. R., Bittman, R., Olkkonen, V. M., & Ikonen, E. (2010) · Traffic · doi:10.1111/j.1600-0854.2010.01142.x · Cited by 106
Lee, O., Chang, C. C. Y., Lee, W., & Chang, T. (1998) · Journal of Lipid Research · doi:10.1016/s0022-2275(20)32203-3 · Cited by 99
Self-citationOxysterols: From physiological tuners to pharmacological opportunities
Brown, A. J., Sharpe, L. J., & Rogers, M. J. (2020) · British Journal of Pharmacology · doi:10.1111/bph.15073 · Cited by 98
Shibuya, Y., Niu, Z., Bryleva, E. Y., Harris, B. T., Murphy, S. R., Kheirollah, A., Bowen, Z. D., Chang, C. C. Y., & Chang, T.-Y. (2015) · Neurobiology of Aging · doi:10.1016/j.neurobiolaging.2015.04.002 · Cited by 87
Self-citationFurukawa, K., Hori, M., Ouchi, N., Kihara, S., Funahashi, T., Matsuzawa, Y., Miyazaki, A., Nakayama, H., & Horiuchi, S. (2004) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2004.03.123 · Cited by 90
Lin, S., Lu, X., Chang, C. C. Y., & Chang, T. (2003) · Molecular Biology of the Cell · doi:10.1091/mbc.e02-11-0725 · Cited by 89
Self-citationLin, S., Cheng, D., Liu, M., Chen, J., & Chang, T. (1999) · Journal of Biological Chemistry · doi:10.1074/jbc.274.33.23276 · Cited by 89
Self-citationConservation of eukaryotic sterol homeostasis: new insights from studies in budding yeast
Sturley, S. L. (2000) · Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids · doi:10.1016/s1388-1981(00)00145-1 · Cited by 86
Guan, C., Niu, Y., Chen, S., Kang, Y., Wu, J.-X., Nishi, K., Chang, C. C. Y., Chang, T., Luo, T., & Chen, L. (2020) · Nature Communications · doi:10.1038/s41467-020-16288-4 · Cited by 86
Self-citationGuo, Z., Song, L., Heinen, J. A., Chang, C. C. Y., & Chang, T. (2005) · Journal of Biological Chemistry · doi:10.1074/jbc.m508384200 · Cited by 81
Self-citationThe Lipid Droplet Knowledge Portal: A resource for systematic analyses of lipid droplet biology
Mejhert, N., Gabriel, K. R., Frendo-Cumbo, S., Krahmer, N., Song, J., Kuruvilla, L., Chitraju, C., Boland, S., Jang, D.-K., von Grotthuss, M., et al. (2022) · Developmental Cell · doi:10.1016/j.devcel.2022.01.003 · Cited by 79
Human Acyl-CoA:Cholesterol Acyltransferase-1 Is a Homotetrameric Enzyme in Intact Cells and in Vitro
Yu, C., Chen, J., Lin, S., Liu, J., Chang, C. C. Y., & Chang, T. (1999) · Journal of Biological Chemistry · doi:10.1074/jbc.274.51.36139 · Cited by 75
Self-citationFujiwara, Y., Kiyota, N., Hori, M., Matsushita, S., Iijima, Y., Aoki, K., Shibata, D., Takeya, M., Ikeda, T., Nohara, T., et al. (2007) · Arteriosclerosis Thrombosis and Vascular Biology · doi:10.1161/atvbaha.107.147405 · Cited by 74
Esterification of okadaic acid in the mussel Mytilus galloprovincialis
Rossignoli, A. E., Fernández, D., Regueiro, J., Mariño, C., & Blanco, J. (2011) · Toxicon · doi:10.1016/j.toxicon.2011.02.003 · Cited by 71
Yang, L. C., Jin Bo, Y. A. N. G., Chen, J., Guang Yao, Y. U., Pei, Z. H. O. U., Lei, L. E. I., Zhen Zhen, W. A. N. G., Chang, C. C. Y., Yang, X., Chang, T., et al. (2004) · Cell Research · doi:10.1038/sj.cr.7290231 · Cited by 70
Self-citationLeptin modulates ACAT1 expression and cholesterol efflux from human macrophages
Hongo, S., Watanabe, T., Arita, S., Kanome, T., Kageyama, H., Shioda, S., & Miyazaki, A. (2009) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.90369.2008 · Cited by 71
Du, X., Pham, Y., & Brown, A. J. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.m408690200 · Cited by 69
Donnelly, K. L., Margosian, M. R., Parks, E. J., Sheth, S. S., & Lusis, A. J. (2004) · Journal of Nutrition · doi:10.1093/jn/134.6.1475 · Cited by 64
Kristiana, I., Sharpe, L. J., Catts, V. S., Lutze-Mann, L. H., & Brown, A. J. (2009) · The Pharmacogenomics Journal · doi:10.1038/tpj.2009.62 · Cited by 64
Fujimoto, T., & Ohsaki, Y. (2006) · Annals of the New York Academy of Sciences · doi:10.1196/annals.1377.010 · Cited by 67
Jensen-Pergakes, K., Guo, Z., Giattina, M., Sturley, S. L., & Bard, M. (2001) · Journal of Bacteriology · doi:10.1128/jb.183.17.4950-4957.2001 · Cited by 64
Molecular Insights into the Mechanisms Underlying the Cholesterol- Lowering Effects of Phytosterols
Cedó, L., Farràs, M., Lee‐Rueckert, M., & Escolá‐Gil, J. C. (2019) · Current Medicinal Chemistry · doi:10.2174/0929867326666190822154701 · Cited by 63
Sakashita, N., Miyazaki, A., Chang, C. C. Y., Chang, T., Kiyota, E., Satoh, M., Komohara, Y., Morganelli, P. M., Horiuchi, S., & Takeya, M. (2003) · Laboratory Investigation · doi:10.1097/01.lab.0000095687.17383.39 · Cited by 62
Self-citationChang, C. C. Y., Dong, R., Miyazaki, A., Sakashita, N., Zhang, Y., Liu, J., Guo, M., Li, B.-L., & Chang, T. (2006) · Acta Biochimica et Biophysica Sinica · doi:10.1111/j.1745-7270.2006.00154.x · Cited by 57
Self-citationImmunolocalization of Acyl-Coenzyme A:CholesterolO-Acyltransferase in Macrophages
Khelef, N., Buton, X., Beatini, N., Wang, H., Meiner, V., Chang, T., Farese, R. V., Maxfield, F. R., & Tabas, I. A. (1998) · Journal of Biological Chemistry · doi:10.1074/jbc.273.18.11218 · Cited by 57
Self-citationFunctional Expression of a cDNA to Human Acyl-coenzyme A:Cholesterol Acyltransferase in Yeast
Yang, H., Cromley, D. A., Wang, H., Billheimer, J. T., & Sturley, S. L. (1997) · Journal of Biological Chemistry · doi:10.1074/jbc.272.7.3980 · Cited by 57
Genes of Cholesterol Metabolism in Human Atheroma
Forcheron, F., Legedz, L., Chinetti, G., Feugier, P., Letexier, D., Bricca, G., & Beylot, M. (2005) · Arteriosclerosis Thrombosis and Vascular Biology · doi:10.1161/01.atv.0000174123.19103.52 · Cited by 54
Guo, Z., Cromley, D. A., Billheimer, J. T., & Sturley, S. L. (2001) · Journal of Lipid Research · doi:10.1016/s0022-2275(20)31579-0 · Cited by 54
Cao, G., Goldstein, J. L., & Brown, M. S. (1996) · Journal of Biological Chemistry · doi:10.1074/jbc.271.24.14642 · Cited by 54
Sliskovic, D. R., Picard, J. A., & Krause, B. R. (2002) · Progress in medicinal chemistry · doi:10.1016/s0079-6468(08)70070-5 · Cited by 52
Fluorescent Sterols and Cholesteryl Esters as Probes for Intracellular Cholesterol Transport
Solanko, K. A., Modzel, M., Solanko, L. M., & Wüstner, D. (2015) · Lipid Insights · doi:10.4137/lpi.s31617 · Cited by 51
Cholesterol oxidation products: other biological effects.
Osada, K., Guardiola, F., Dutta, P. C., Codony, R., & Savage, G. P. (2002) · Cited by 47
Kusunoki, J., Aragane, K., Kitamine, T., Kozono, H., Kano, K., Fujinami, K., Kojima, K., Chiwata, T., & Sekine, Y. (2000) · Arteriosclerosis Thrombosis and Vascular Biology · doi:10.1161/01.atv.20.1.171 · Cited by 47
Yang, J.-B., Duan, Z., Yao, W., Lee, O., Yang, L. C., Yang, X., Sun, X., Chang, C. C. Y., Chang, T., & Li, B.-L. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m011488200 · Cited by 46
Self-citationXie, C., Woollett, L. A., Turley, S. D., & Dietschy, J. M. (2002) · Journal of Lipid Research · doi:10.1194/jlr.m200146-jlr200 · Cited by 45
Khelef, N., Soe, T. T., Quehenberger, O., Beatini, N., Tabas, I. A., & Maxfield, F. R. (2000) · Arteriosclerosis Thrombosis and Vascular Biology · doi:10.1161/01.atv.20.7.1769 · Cited by 44
Spady, D. K., Willard, M. N., & Meidell, R. S. (2000) · Journal of Biological Chemistry · doi:10.1074/jbc.m005097200 · Cited by 43
Lipid droplets diversity and functions in inflammation and immune response
Pereira-Dutra, F. S., & Bozza, P. T. (2021) · Expert Review of Proteomics · doi:10.1080/14789450.2021.1995356 · Cited by 40
Acute ACAT1/SOAT1 Blockade Increases MAM Cholesterol and Strengthens ER-Mitochondria Connectivity
Harned, T. C., Stan, R. V., Cao, Z., Chakrabarti, R., Higgs, H. N., Chang, C. C. Y., & Chang, T. (2023) · International Journal of Molecular Sciences · doi:10.3390/ijms24065525 · Cited by 41
Self-citationACAT inhibitors: evolution from cholesterol-absorption inhibitors to antiatherosclerotic agents
Roth, B. D. (1998) · Drug Discovery Today · doi:10.1016/s1359-6446(97)01123-9 · Cited by 40
Cho, K., An, S., Lee, W.-S., Paik, Y., Kim, Y. K., & Jeong, T. (2003) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2003.08.077 · Cited by 39
Heisler, D. B., Johnson, K. A., Ohlson, M. B., Ma, D. H., Zhang, L., Tran, M., Corley, C. D., Abrams, M. E., McDonald, J. G., Schoggins, J. W., et al. (2023) · eLife · doi:10.7554/elife.83534 · Cited by 40
Shen, H., Howles, P. N., & Tso, P. (2001) · Advanced Drug Delivery Reviews · doi:10.1016/s0169-409x(01)00181-8 · Cited by 37
Farese, R. V. (1998) · Current Opinion in Lipidology · doi:10.1097/00041433-199804000-00007 · Cited by 36
Kristiana, I., Yang, H., & Brown, A. J. (2008) · Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids · doi:10.1016/j.bbalip.2008.08.006 · Cited by 35
Sakashita, N., Chang, C. C. Y., Lei, X., Fujiwara, Y., Takeya, M., & Chang, T. (2009) · Journal of Lipid Research · doi:10.1194/jlr.m900288-jlr200 · Cited by 33
Self-citationFactors Affecting Intestinal Absorption of Cholesterol and Plant Sterols and Stanols
Ikeda, I. (2015) · Journal of Oleo Science · doi:10.5650/jos.ess14221 · Cited by 33
Ohmoto, T., Nishitsuji, K., Yoshitani, N., Mizuguchi, M., Yanagisawa, Y., Saito, H., & Sakashita, N. (2015) · Molecular Medicine Reports · doi:10.3892/mmr.2015.4200 · Cited by 34
Yang, L. C., Lee, O., Chen, J., Chen, J., Chang, C. C. Y., Zhou, P., Wang, Z., Ma, H., Sha, H., Feng, J.-X., et al. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.m408155200 · Cited by 32
Self-citationLee, J., & Carr, T. P. (2004) · Journal of Nutrition · doi:10.1093/jn/134.12.3239 · Cited by 32
Mechanisms Underlying the Health Benefits of Plant Sterol and Stanol Ester Consumption
Plat, J., Baumgartner, S., & Mensink, R. P. (2015) · Journal of AOAC International · doi:10.5740/jaoacint.sgeplat · Cited by 31
Junquero, D. C., Oms, P., Carilla-Durand, E., Autin, J.-M., Tarayre, J.-P., Degryse, A. A. Y., Patoiseau, J., Colpaert, F. C., & Delhon, A. (2001) · Biochemical Pharmacology · doi:10.1016/s0006-2952(00)00523-2 · Cited by 31
Rogers, M. A., Chang, C. C. Y., Maue, R. A., Melton, E. M., Peden, A. A., Garver, W. S., Lee, J., Schroen, P. W., Huang, M. M., & Chang, T. (2022) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2201646119 · Cited by 27
Self-citationJunquero, D. C., Bruniquel, F., Nguyễn, X. H., Autin, J.-M., Patoiseau, J., Degryse, A.-D., Colpaert, F. C., & Delhon, A. (2001) · Atherosclerosis · doi:10.1016/s0021-9150(00)00559-1 · Cited by 29
Maung, K. K., Miyazaki, A., Nomiyama, H., Chang, C. C. Y., Chang, T., & Horiuchi, S. (2001) · Journal of Lipid Research · doi:10.1016/s0022-2275(20)31677-1 · Cited by 29
Self-citation
Metadata
- Authors
- Catherine C. Y., C., Chen, J., Thomas, M. A., Cheng, D., Veronica A., D. P., Newton, R. S., Pape, M. E., & Chang, T.-Y.
- Affiliations
- Dartmouth College (US)
- Corresponding author
- Chang, Ta-Yuan
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1995-12
- Volume
- 270
- Issue
- 49
- Pages
- 29532-29540
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1996953344
- Cited by
- 158
- References
- 45
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 1 · 2023: 4 · 2022: 3 · 2021: 3 · 2020: 4 · 2019: 3 · 2016: 1 · 2015: 7 · 2014: 6 · 2013: 1 · 2012: 3
- FWCI
- 10.33
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Biological Chemistry (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health
- National Heart, Lung, and Blood Institute (HL 36709)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Cholesterol and Lipid Metabolism
- Physical Sciences › Chemistry › Organic Chemistry › Steroid Chemistry and Biochemistry
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Keywords
- acyl-CoA:cholesterol acyltransferase, ACAT, polyclonal antibodies, immunolocalization, endoplasmic reticulum, cholesterol esterification, immunodepletion, human cell lines, CHO cells, HepG2 cells, fibroblasts
- MeSH terms
- Animals, Antibodies, Cell Line, Sterol O-Acyltransferase, Endoplasmic Reticulum, Cricetinae, Humans, Molecular Weight, Rabbits, Immunoblotting, CHO Cells
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