The DOI matches its Crossref record; 36 of the 36 references it cites were found in a registry or an index.
Stat1 Depends on Transcriptional Synergy with Sp1
Open accessAbstract
STAT (signal transducer and activator of transcription) proteins combine with cytokine receptors and receptor-associated kinases in distinct protein/protein interactions that are critical for STAT-dependent signal transduction events, but the nature of any subsequent STAT interactions with DNA-binding proteins in the nucleus is less certain. Based on assays of DNA/protein binding and activity of transfected reporter plasmids, we determined that occupation of contiguous DNA-binding sites for Stat1 (the first member of the STAT family) and the transcriptional activator Sp1 are both required for full activation of the intercellular adhesion molecule-1 gene by interferon-gamma. Thus, Stat1 binding to DNA cannot by itself be equated with biologic actions of Stat1. In co-immunoprecipitation experiments, we also obtained evidence of direct and selective Stat1/Sp1 interaction (in primary culture cells without overexpression), further indicating that Stat1/Sp1 synergy confers an element of specificity in the pathway leading to cytokine-activated transcription and cytokine-dependent immunity and inflammation.
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“Although they bind to a similar DNA binding site, STATs may interact with co-activator or co-repressor to regulate different sets of their own downstream targets (5”
From STAT3 Contributes to the Mitogenic Response of Hepatocytes during Liver Regeneration (2002) · cites this paper as [38]
Citations
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HOW CELLS RESPOND TO INTERFERONS
Stark, G. R., Kerr, I. M., Williams, B. R. G., Silverman, R. H., & Schreiber, R. D. (1998) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.67.1.227 · Cited by 3,932
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Metadata
- Authors
- Look, D. C., Pelletier, M. R., Tidwell, R. M., Roswit, W. T., & Holtzman, M. J.
- Affiliations
- Washington University in St. Louis (US)
- Corresponding author
- Holtzman, Michael J.
- ORCID
- Holtzman, Michael J.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1995-12
- Volume
- 270
- Issue
- 51
- Pages
- 30264-30267
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2129362876
- Cited by
- 268
- References
- 36
- Citations by year
- 2026: 1 · 2025: 1 · 2023: 1 · 2022: 1 · 2021: 2 · 2020: 3 · 2019: 2 · 2018: 2 · 2017: 2 · 2016: 4 · 2015: 3 · 2014: 1 · 2013: 2 · 2012: 5
- FWCI
- 8.74
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Biological Chemistry (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (5r01hl040078-10)
- National Heart, Lung, and Blood Institute (HL-40078, HL 07317, HL/AI-51071, T32HL007317)
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › Cytokine Signaling Pathways and Interactions
- Life Sciences › Immunology and Microbiology › Immunology › interferon and immune responses
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Keywords
- STAT1, Sp1, transcriptional synergy, interferon-gamma, DNA binding sites, transcriptional activation, cytokine signaling
- MeSH terms
- Animals, Base Sequence, Binding Sites, Cell Line, DNA-Binding Proteins, Humans, Interferon-gamma, Luciferases, Molecular Sequence Data, Oligodeoxyribonucleotides, Recombinant Fusion Proteins, Recombinant Proteins, Repetitive Sequences, Nucleic Acid, Transcription, Genetic, Transfection, Signal Transduction, Chloramphenicol O-Acetyltransferase, Trans-Activators, Gene Expression, Polymerase Chain Reaction, Intercellular Adhesion Molecule-1, STAT1 Transcription Factor
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