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AP-4, a Novel Protein Complex Related to Clathrin Adaptors
Open accessAbstract
Here we report the identification and characterization of AP-4, a novel protein complex related to the heterotetrameric AP-1, AP-2, and AP-3 adaptors that mediate protein sorting in the endocytic and late secretory pathways. The key to the identification of this complex was the cloning and sequencing of two widely expressed, mammalian cDNAs encoding new homologs of the adaptor beta and sigma subunits named beta4 and sigma4, respectively. An antibody to beta4 recognized in human cells an approximately 83-kDa polypeptide that exists in both soluble and membrane-associated forms. Gel filtration, sedimentation velocity, and immunoprecipitation experiments revealed that beta4 is a component of a multisubunit complex (AP-4) that also contains the sigma4 polypeptide and two additional adaptor subunit homologs named mu4 (mu-ARP2) and epsilon. Immunofluorescence analyses showed that AP-4 is associated with the trans-Golgi network or an adjacent structure and that this association is sensitive to the drug brefeldin A. We propose that, like the related AP-1, AP-2, and AP-3 complexes, AP-4 plays a role in signal-mediated trafficking of integral membrane proteins in mammalian cells.
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Cited by
Braulke, T., & Bonifacino, J. S. (2009) · Biochimica et Biophysica Acta (BBA) - Molecular Cell Research · doi:10.1016/j.bbamcr.2008.10.016 · Cited by 811
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A Novel Clathrin Adaptor Complex Mediates Basolateral Targeting in Polarized Epithelial Cells
Fölsch, H., Ohno, H., Bonifacino, J. S., & Mellman, I. (1999) · Cell · doi:10.1016/s0092-8674(00)81650-5 · Cited by 467
Self-citationAdaptors for Clathrin-Mediated Traffic
Kirchhausen, T. (1999) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev.cellbio.15.1.705 · Cited by 427
Multicolour imaging of post-Golgi sorting and trafficking in live cells
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Lee, B. L., Moon, J. E., Shu, J. H., Yuan, L., Newman, Z. R., Schekman, R., & Barton, G. M. (2013) · eLife · doi:10.7554/elife.00291 · Cited by 281
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Structure and interactions of the endogenous human Commander complex
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Metadata
- Authors
- Dell'Angelica, E. C., Mullins, C., & Bonifacino, J. S.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1999-03
- Volume
- 274
- Issue
- 11
- Pages
- 7278-7285
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1536338076
- Cited by
- 293
- References
- 0
- FWCI
- 10.57
- Type
- journal article
- Language
- en
- Open access
- hybrid
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