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Cited by 205

The DOI matches its Crossref record; 4 of the 4 references it cites were found in a registry or an index.

Maturation and Pro-peptide Cleavage of β-Secretase

Capell, Anja, Steiner, Harald, Willem, Michael, Kaiser, Hartmut, Meyer, Carmen, Walter, Jochen, Lammich, Sven, Multhaup, Gerd, Haass, Christian·2000·Journal of Biological Chemistry
DOI 10.1074/jbc.m003202200
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Abstract

Amyloid beta-peptide is generated by two sequential proteolytic cleavages mediated by beta-secretase (BACE) and gamma-secretase. BACE was recently identified as a membrane-associated aspartyl protease. We have now analyzed the maturation and pro-peptide cleavage of BACE. Pulse-chase experiments revealed that BACE is post-translationally modified during transport to the cell surface, which can be monitored by a significant increase in the molecular mass. The increase in molecular mass is caused by complex N-glycosylation. Treatment with tunicamycin and N-glycosidase F led to a BACE derivative with a molecular weight corresponding to an unmodified version. In contrast, the mature form of BACE was resistant to endoglycosidase H treatment. The cytoplasmic tail of BACE was required for efficient maturation and trafficking through the Golgi; a BACE variant lacking the cytoplasmic tail undergoes inefficient maturation. In contrast a soluble BACE variant that does not contain a membrane anchor matured more rapidly than full-length BACE. Pro-BACE was predominantly located within the endoplasmic reticulum. Pro-peptide cleavage occurred immediately before full maturation and trafficking through the Golgi.

Overview

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Crossref · 10.1074/jbc.m003202200

Cites

4 of 4 references verified0 citation errors · 0 not checkable

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  • Overexpression of BACE1 strongly increases β-secretory cleavage of APP already in early secretory compartments.

    “Indeed, it has been shown that overexpression of BACE1 strongly increases ␤-secretory cleavage of APP already in early secretory compartments (Capell et al., 2000;Liu et al., 2002;Lee et al., 2005)”

    From GGA1 Is Expressed in the Human Brain and Affects the Generation of Amyloid β-Peptide (2006) · cites this paper as Capell et al., 2000

Citations

Cites

Flagged first, then by citations

Research works printed but not linked: 28

  1. Selkoe, 1999, Nature, 399, A23, 10.1038/399a023 · DOI · Paper page
  2. Haass, 1993, Cell, 75, 1039, 10.1016/0092-8674(93)90312-E · DOI · Paper page
  3. Weidemann, 1989, Cell, 57, 115, 10.1016/0092-8674(89)90177-3 · DOI
  4. Hung, 1994, EMBO J., 13, 534, 10.1002/j.1460-2075.1994.tb06291.x · DOI
  5. Walter, 1997, J. Biol. Chem., 272, 1896, 10.1074/jbc.272.3.1896 · DOI
  6. Perez, 1999, J. Biol. Chem., 274, 18851, 10.1074/jbc.274.27.18851 · DOI
  7. Haass, 1992, Nature, 357, 500, 10.1038/357500a0 · DOI
  8. Yamazaki, 1996, J. Cell Sci., 109, 999, 10.1242/jcs.109.5.999 · DOI
  9. Golde, 1992, Science, 255, 728, 10.1126/science.1738847 · DOI
  10. Haass, 1994, Proc. Natl. Acad. Sci. U. S. A., 91, 1564, 10.1073/pnas.91.4.1564 · DOI
  11. De Strooper, 1995, J. Biol. Chem., 270, 4058, 10.1074/jbc.270.8.4058 · DOI
  12. Yamazaki, 1997, J. Neurosci., 17, 1004, 10.1523/JNEUROSCI.17-03-01004.1997 · DOI
  13. Esch, 1990, Science, 248, 1122, 10.1126/science.2111583 · DOI
  14. Skovronsky, 2000, J. Biol. Chem., 275, 2568, 10.1074/jbc.275.4.2568 · DOI
  15. Thinakaran, 1996, J. Biol. Chem., 271, 9390, 10.1074/jbc.271.16.9390 · DOI · Paper page
  16. Lammich, 1999, Proc. Natl. Acad. Sci. U. S. A., 96, 3922, 10.1073/pnas.96.7.3922 · DOI · Paper page
  17. Buxbaum, 1998, J. Biol. Chem., 273, 27765, 10.1074/jbc.273.43.27765 · DOI
  18. Wolfe, 1999, Nature, 398, 513, 10.1038/19077 · DOI
  19. Yan, 1999, Nature, 402, 533, 10.1038/990107 · DOI · Paper page
  20. Lin, 2000, Proc. Natl. Acad. Sci. U. S. A., 97, 1456, 10.1073/pnas.97.4.1456 · DOI
  21. Sinha, 1999, Nature, 402, 537, 10.1038/990114 · DOI · Paper page
  22. Hussain, 1999, Mol. Cell Neurosci., 14, 419, 10.1006/mcne.1999.0811 · DOI · Paper page
  23. Saunders, 1999, Science, 286, 1255a, 10.1126/science.286.5443.1255a · DOI
  24. Fan, 1999, Science, 286, 1255a, 10.1126/science.286.5443.1255a · DOI
  25. Bennet, 2000, J. Biol. Chem., 275, 20647, 10.1074/jbc.M002688200 · DOI · Paper page
  26. Haass, 1991, J. Neurosci., 11, 3783, 10.1523/JNEUROSCI.11-12-03783.1991 · DOI
  27. Wild-Bode, 1997, J. Biol. Chem., 272, 16085, 10.1074/jbc.272.26.16085 · DOI · Paper page
  28. Nakayama, 1997, Biochem. J., 327, 625, 10.1042/bj3270625 · DOI

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Metadata

Authors
Capell, A., Steiner, H., Willem, M., Kaiser, H., Meyer, C., Walter, J., Lammich, S., Multhaup, G., & Haass, C.
Venue
Journal of Biological Chemistry
Publisher
Elsevier BV
Published
2000-10
Volume
275
Issue
40
Pages
30849-30854
ISSN
0021-9258
DOI
10.1074/jbc.m003202200
Cited by
205
References
4
Language
en
Licence

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