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Cited by 45interferon and immune responsesCytokine Signaling Pathways and InteractionsGenomics and Chromatin Dynamics

Interaction between Interferon Consensus Sequence-binding Protein and COP9/Signalosome Subunit CSN2 (Trip15)

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Cohen, Helit, Azriel, Aviva, Cohen, Tali, Meraro, David, Hashmueli, Sharon, Bech-Otschir, Dawadschargal, Kraft, Regine, Dubiel, Wolfgang +1 more·2000·Journal of Biological Chemistry
DOI 10.1074/jbc.m004900200
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Abstract

Interferon consensus sequence-binding protein (ICSBP) is a member of the interferon regulatory factors (IRF) that has a pivotal role in mediating resistance to pathogenic infections in mice and in promoting the differentiation of myeloid cells. ICSBP exerts some of its transcriptional activities via association with other factors that enable its binding to a variety of promoters containing DNA composite elements. These interactions are mediated through a specific COOH-terminal domain termed IAD (IRF association domain). To gain a broader insight of the capacity of ICSBP to interact with other factors, yeast two-hybrid screens were performed using ICSBP-IAD as a bait against a B-cell cDNA library. Trip15 was identified as a specific interacting factor with ICSBP in yeast cells, which was also confirmed by in vitro glutathione S-transferase pull-down assays and by coimmunoprecipitation studies in COS7 cells. Trip15 was recently identified as a component of the COP9/signalosome (CSN) complex composed of eight evolutionary conserved subunits and thus termed CSN2. This complex has a role in cell-signaling processes, which is manifested by its associated novel kinase activity and by the involvement of its subunits in regulating multiple cell-signaling pathways and cell-cycle progression. We show that in vitro association of ICSBP with the CSN leads to phosphorylation of ICSBP at a unique serine residue within its IAD. The phosphorylated residue is essential for efficient association with IRF-1 and thus for the repressor activity of ICSBP exerted on IRF-1. This suggests that the CSN has a role in integrating incoming signals that affect the transcriptional activity of ICSBP.

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  • ICSBP binds Trip15 (CSN2), a component of the COP9/signalosome (CSN) complex, and the CSN complex phosphorylates a serine residue within the IAD of ICSBP that is essential for efficient association with IRF-1.

    “ICSBP was shown to bind Trip15 (CSN2), which is a component of the COP9/signalosome (CSN) complex.”

    From Review: ICSBP/IRF-8: Its Regulatory Roles in the Development of Myeloid Cells (2002) · cites this paper as [36]

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Authors
Cohen, H., Azriel, A., Cohen, T., Meraro, D., Hashmueli, S., Bech-Otschir, D., Kraft, R., Dubiel, W., & Levi, B.-Z.
Affiliations
  • Technion – Israel Institute of Technology (IL)
  • Max Delbrück Center (DE)
  • Humboldt-Universität zu Berlin (DE)
Corresponding author
Levi, Ben-Zion
ORCID
Venue
Journal of Biological Chemistry
Publisher
Elsevier BV
Published
2000-12
Volume
275
Issue
50
Pages
39081-39089
ISSN
0021-9258 (ISSN-L)
DOI
10.1074/jbc.m004900200
OpenAlex
W2135480993
Cited by
45
References
51
Citations by year
2022: 1 · 2021: 1 · 2019: 1 · 2018: 1 · 2016: 2 · 2015: 1 · 2014: 1 · 2013: 1 · 2012: 1
FWCI
2.71
Type
journal article
Language
en
Open access
Licence
Locations
  • Journal of Biological Chemistry (publishedVersion, cc-by)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Immunology and Microbiology › Immunology › interferon and immune responses
  • Health Sciences › Medicine › Oncology › Cytokine Signaling Pathways and Interactions
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
Keywords
IRF1, COP9 signalosome, β-casein, protein-protein interaction, serine phosphorylation, transcriptional repression, interferon regulatory factors, signal integration
MeSH terms
COP9 Signalosome Complex, Interferon Regulatory Factor-8, Alanine, Animals, B-Lymphocytes, Carrier Proteins, DNA, Dose-Response Relationship, Drug, Glutathione Transferase, HeLa Cells, Humans, Models, Biological, Nuclear Proteins, Peptide Hydrolases, Phosphorylation, Plasmids, Precipitin Tests, Promoter Regions, Genetic, Protein Binding, Proteins, Receptors, Thyroid Hormone, Repressor Proteins, RNA, Messenger, Serine, Transcription Factors, Transcription, Genetic, Blotting, Northern, Signal Transduction, Gene Library, Mutagenesis, Site-Directed, 3T3 Cells, Protein Structure, Tertiary, DNA, Complementary, HL-60 Cells, COS Cells, Two-Hybrid System Techniques, Multiprotein Complexes, Interferon Regulatory Factors, Mice

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