Molecular interactions between interferon consensus sequence binding protein and members of the interferon regulatory factor family.
Open accessAbstract
Interferon (IFN) consensus sequence binding protein (ICSBP) is a transcription factor expressed mostly in the cells of the immune system. ICSBP belongs to the IFN regulatory factor (IRF) family, which also includes IRF-1, IRF-2, and the IFN-alpha-stimulated gene factor 3 gamma (ISGF3 gamma). We show here that ICSBP forms a complex with IRF-1 or IRF-2 both in vivo and in vitro and, in the presence or absence of the target DNA, with the IFN-stimulated response element (ISRE). Further, electrophoretic mobility shift assays show that this interaction greatly enhances the otherwise very low binding affinity of ICSBP to the ISRE. We show, on the other hand, that ICSBP inhibits binding of the IFN-alpha-stimulated gene factor 3 gamma to the ISRE. Through these interactions ICSBP is likely to exert complex modulatory functions in the regulation of IFN-stimulated genes.
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- Authors
- Bovolenta, C., Driggers, P. H., Marks, M. S., Medin, J. A., Politis, A. D., Vogel, S. N., Levy, D. E., Sakaguchi, K., Appella, E., & Coligan, J. E.
- Affiliations
- National Institutes of Health (US)
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 1994-05-24
- Volume
- 91
- Issue
- 11
- Pages
- 5046-5050
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC43928
- OpenAlex
- W2102204735
- Cited by
- 167
- References
- 37
- Citations by year
- 2026: 3 · 2025: 2 · 2023: 4 · 2022: 6 · 2021: 6 · 2020: 5 · 2019: 5 · 2018: 2 · 2017: 2 · 2016: 4 · 2015: 2 · 2014: 2 · 2013: 1 · 2012: 5
- FWCI
- 4.71
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (AI18797)
- National Institute of Allergy and Infectious Diseases (AI-18797)
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › interferon and immune responses
- Health Sciences › Medicine › Oncology › Cytokine Signaling Pathways and Interactions
- Life Sciences › Immunology and Microbiology › Immunology › Immune Response and Inflammation
- Keywords
- IRF8, IRF1, IRF2, interferon-stimulated response element, transcription factor-DNA binding, protein-protein interaction, transcriptional regulation
- MeSH terms
- Interferon Regulatory Factor-8, Base Sequence, Carrier Proteins, Cell Line, DNA, DNA-Binding Proteins, Humans, Molecular Sequence Data, Phosphoproteins, Precipitin Tests, Protein Binding, Repressor Proteins, Transcription Factors, Consensus Sequence, Interferon-Stimulated Gene Factor 3, Interferon-Stimulated Gene Factor 3, gamma Subunit, Interferon Regulatory Factors, Interferon Regulatory Factor-2, Interferon Regulatory Factor-1
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