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Recognition of α2-Macroglobulin by the Low Density Lipoprotein Receptor-related Protein Requires the Cooperation of Two Ligand Binding Cluster Regions
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BACE immunoprecipitated from co-transfected H4 cells pulls down mLRP1-Myc, with immunoreactive bands of approximately 100 kDa and 140 kDa.
“Immunoreactive bands of ϳ100 kDa (resembling mature furin-cleaved mLRP1-Myc) and ϳ140 kDa (resembling unprocessed Golgi and ER forms of mLRP1-Myc) (29) were detected in the immunoprecipitated sample and the whole cell lysate (Fig. 3A, lane 4).”
From The Low Density Lipoprotein Receptor-related Protein (LRP) Is a Novel β-Secretase (BACE1) Substrate (2005) · cites this paper as [29]
The passage headed “High Affinity α2M* Binding to LRP Requires Ligand Binding Repeats in Both Cluster I and Cluster II” (page unavailable) reports that Myc-tagged mini-receptors, including mLRP1, were processed by furin. It does not identify approximately 100-kDa and 140-kDa bands as mature and unprocessed mLRP1-Myc forms. The BACE immunoprecipitation is presented as the citing paper’s own result, not as a result attributed to this source. (LLM, conf=0.83)
Experimental — accuracy not yet measuredEndogenous LRP and BACE co-immunoprecipitate from human brain tissue.
“Thus, endogenous LRP and BACE co-immunoprecipitate.”
From The Low Density Lipoprotein Receptor-related Protein (LRP) Is a Novel β-Secretase (BACE1) Substrate (2005) · cites this paper as [29]
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Cites
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The Low Density Lipoprotein Receptor-related Protein (LRP) Is a Novel β-Secretase (BACE1) Substrate
von Arnim, C. A. F., Kinoshita, A., Peltan, I. D., Tangredi, M. M., Herl, L., Lee, B. M., Spoelgen, R., Hshieh, T. T., Ranganathan, S., Battey, F. D., et al. (2005) · Journal of Biological Chemistry · doi:10.1074/jbc.m414248200 · Cited by 258
Self-citationGLIEMANN, J., HERMEY, G., NYKJÆR, A., PETERSEN, C. M., JACOBSEN, C., & ANDREASEN, P. A. (2004) · Biochemical Journal · doi:10.1042/bj20040149 · Cited by 58
Ranganathan, S., Noyes, N. C., Migliorini, M., Winkles, J. A., Battey, F. D., Hyman, B. T., Smith, E., Yepes, M., Mikhailenko, I., & Strickland, D. K. (2011) · Journal of Neuroscience · doi:10.1523/jneurosci.5065-10.2011 · Cited by 49
“Previous studies have demonstrated that LRP1 mini-receptors function like LRP1 to internalize ligands and represent good models for LRP1-mediated endocytosis (Willnow et al., 1994; Mikhailenko et al., 2001).”
BackgroundSelf-citation
Metadata
- Authors
- Mikhailenko, I., Battey, F. D., Migliorini, M., Ruiz, J. F., Argraves, K., Moayeri, M., & Strickland, D. K.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 2001-10
- Volume
- 276
- Issue
- 42
- Pages
- 39484-39491
- ISSN
- 0021-9258
- Cited by
- 70
- References
- 3
- Language
- en
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