CoChat
Sign Up
Cited by 110

The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.

Dissection of receptor folding and ligand-binding property with functional minireceptors of LDL receptor-related protein

Obermoeller-McCormick, Lynn M., Li, Yonghe, Osaka, Hiroshi, FitzGerald, David J., Schwartz, Alan L., Bu, Guojun·2001·Journal of Cell Science
DOI 10.1242/jcs.114.5.899
Open this paper in CoChat — free account

Abstract

The LDL receptor-related protein (LRP) is a large, multifunctional endocytic receptor that binds and endocytoses a variety of structurally and functionally distinct ligands. LRP contains four putative ligand-binding domains. However, only domains II, III and IV, but not domain I, bind the receptor-associated protein (RAP), a molecular chaperone and universal antagonist for LRP. In order to dissect the function of RAP in LRP folding and to examine the ligand-binding properties of LRP, we generated LRP minireceptors that represent each of the four putative ligand-binding domains (termed mLRP1, mLRP2, mLRP3 and mLRP4, respectively). We found that proper folding and trafficking of mLRP2, mLRP3, mLRP4, but not mLRP1, is facilitated by coexpression of RAP. When these mLRPs were stably expressed in Chinese Hamster Ovary cells that lack the endogenous LRP, we found that each of these receptors was processed and traffics through the secretory pathway. Cell surface expression of these minireceptors was quantitatively examined by flow cytometric analyses. Using these minireceptor cell lines to map the ligand-binding domains, we found that although the majority of LRP ligands bind to both domain II and domain IV, Pseudomonas exotoxin A utilizes only domain IV for its binding to LRP. We conclude that while domains II and IV of LRP share many ligand-binding properties, each of the putative ligand-binding domains of LRP is unique in its contribution to ligand binding.

Overview

Integrity signals

Registry

Crossref · 10.1242/jcs.114.5.899

Cites

1 of 1 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Citations

Cites

Flagged first, then by citations

Research works printed but not linked: 33

  1. Bu, 1998, Receptor-associated protein: a specialized chaperone and antagonist for members of the LDL receptor gene family, Curr. Opin. Lipidol, 9, 149, 10.1097/00041433-199804000-00012 · DOI
  2. Bu, 1995, 39 kDa receptor-associated protein is an ER resident protein and molecular chaperone for LDL receptor-related protein, EMBO J, 14, 2269, 10.1002/j.1460-2075.1995.tb07221.x · DOI · Paper page
  3. Bu, 1994, Subcellular localization and endocytic function of low density lipoprotein receptor-related protein in human glioblastoma cells, J. Biol. Chem, 269, 29874, 10.1016/S0021-9258(18)43962-2 · DOI
  4. Bu, 1993, Receptor-mediated endocytosis of tissue-type plasminogen activator by low density lipoprotein receptor-related protein on human hepatoma HepG2 cells, J. Biol. Chem, 268, 13002, 10.1016/S0021-9258(18)31486-8 · DOI
  5. Bu, 1992, Identification and partial characterization by chemical cross-linking of a binding protein for tissue-type plasminogen activator (t-PA) on rat hepatoma cells. A plasminogen activator inhibitor type 1-independent t-PA receptor, J. Biol. Chem, 267, 15595, 10.1016/S0021-9258(19)49578-1 · DOI
  6. Bu, 1996, Receptor-associated protein is a folding chaperone for low density lipoprotein receptor-related protein, J. Biol. Chem, 271, 22218, 10.1074/jbc.271.36.22218 · DOI · Paper page
  7. Bu, 1998, RAP, a novel type of ER chaperone, Trends Cell Biol, 8, 272, 10.1016/S0962-8924(98)01283-5 · DOI · Paper page
  8. Bu, 1992, Low density lipoprotein receptor-related protein/alpha 2-macroglobulin receptor is an hepatic receptor for tissue-type plasminogen activator, Proc. Natl. Acad. Sci. USA, 89, 7427, 10.1073/pnas.89.16.7427 · DOI
  9. FitzGerald, 1995, Pseudomonas exotoxin-mediated selection yields cells with altered expression of low-density lipoprotein receptor-related protein, J. Cell Biol, 129, 1533, 10.1083/jcb.129.6.1533 · DOI
  10. Goretzki, 1998, Low-density-lipoprotein-receptor- related protein (LRP) interacts with a GTP-binding protein, Biochem. J, 336, 381, 10.1042/bj3360381 · DOI · Paper page
  11. Herz, 1988, Surface location and high affinity for calcium of a 500-kd liver membrane protein closely related to the LDL-receptor suggest a physiological role as lipoprotein receptor, EMBO J, 7, 4119, 10.1002/j.1460-2075.1988.tb03306.x · DOI
  12. Herz, 1990, Proteolytic processing of the 600 kd low density lipoprotein receptor-related protein (LRP) occurs in a trans-Golgi compartment, EMBO J, 9, 1769, 10.1002/j.1460-2075.1990.tb08301.x · DOI
  13. Hussain, 1999, The mammalian low-density lipoprotein receptor family, Annu. Rev. Nutr, 19, 141, 10.1146/annurev.nutr.19.1.141 · DOI
  14. Kounnas, 1992, The alpha2-macroglobulin receptor/low density lipoprotein receptor-related protein binds and internalizes Pseudomonas exotoxin A, J. Biol. Chem, 267, 12420, 10.1016/S0021-9258(18)42291-0 · DOI
  15. Krieger, 1994, Structures and functions of multiligand lipoprotein receptors: macrophage scavenger receptors and LDL receptor-related protein (LRP), Annu. Rev. Biochem, 63, 601, 10.1146/annurev.bi.63.070194.003125 · DOI
  16. Laemmli, 1970, Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature, 227, 680, 10.1038/227680a0 · DOI · Paper page
  17. Li, 2000, The YXXL motif, but not the two NPXY motifs, serves as the dominant endocytosis signal for LDL receptor-related protein (LRP), J. Biol. Chem, 275, 17187, 10.1074/jbc.M000490200 · DOI
  18. Li, 1997, Expression of alpha 2 macroglobulin receptor/low density lipoprotein receptor-related protein is cell culture density-dependent in human breast cancer cell line BT-20, Biochem. Biophys. Res. Comm, 240, 122, 10.1006/bbrc.1997.7619 · DOI
  19. Misra, 1999, Ligation of low-density lipoprotein receptor-related protein with antibodies elevates intracellular calcium and inositol 1,4,5-trisphosphate in macrophages, Arch. Biochem. Biophys, 372, 238, 10.1006/abbi.1999.1521 · DOI
  20. Moestrup, 1993, Alpha 2-macroglobulin-proteinase complexes, plasminogen activator inhibitor type-1-plasminogen activator complexes, and receptor-associated protein bind to a region of the alpha 2-macroglobulin receptor containing a cluster of eight complement-type repeats, J. Biol. Chem, 268, 13691, 10.1016/S0021-9258(19)38702-2 · DOI
  21. Neels, 1999, The second and fourth cluster of class A cysteine-rich repeats of the low density lipoprotein receptor-related protein share ligand-binding properties, J. Biol. Chem, 274, 31305, 10.1074/jbc.274.44.31305 · DOI · Paper page
  22. Obermoeller, 1998, Ca2+ and receptor-associated protein are independently required for proper folding and disulfide bond formation of the low density lipoprotein receptor-related protein, J. Biol. Chem, 273, 22374, 10.1074/jbc.273.35.22374 · DOI
  23. Saito, 1994, Complete cloning and sequencing of rat gp330/’megalin’, a distinctive member of the low density lipoprotein receptor gene family, Proc. Natl. Acad. Sci. USA, 91, 9725, 10.1073/pnas.91.21.9725 · DOI
  24. Strickland, 1991, Primary structure of alpha 2-macroglobulin receptor-associated protein. Human homologue of a Heymann nephritis antigen, J. Biol. Chem, 266, 13364, 10.1016/S0021-9258(18)98848-4 · DOI
  25. Trommsdorff, 1999, Reeler/Disabled-like disruption of neuronal migration in knockout mice lacking the VLDL receptor and ApoE receptor 2, Cell, 97, 689, 10.1016/S0092-8674(00)80782-5 · DOI · Paper page
  26. Trommsdorff, 1998, Interaction of cytosolic adaptor proteins with neuronal apolipoprotein E receptors and the amyloid precursor protein, J. Biol. Chem, 273, 33556, 10.1074/jbc.273.50.33556 · DOI · Paper page
  27. Warshawsky, 1994, The low density lipoprotein receptor-related protein mediates the cellular degradation of tissue factor pathway inhibitor, Proc. Natl. Acad. Sci. USA, 91, 6664, 10.1073/pnas.91.14.6664 · DOI
  28. Willnow, 1995, Functional expression of low density lipoprotein receptor-related protein is controlled by receptor-associated protein in vivo, Proc. Natl. Acad. Sci. USA, 92, 4537, 10.1073/pnas.92.10.4537 · DOI · Paper page
  29. Willnow, 1994, Genetic deficiency in low density lipoprotein receptor-related protein confers cellular resistance to Pseudomonas exotoxin A. Evidence that this protein is required for uptake and degradation of multiple ligands, J. Cell Sci, 107, 719, 10.1242/jcs.107.3.719 · DOI
  30. Willnow, 1996, The low-density-lipoprotein receptor-related protein (LRP) is processed by furin in vivo and in vitro, Biochem. J, 313, 71, 10.1042/bj3130071 · DOI
  31. Willnow, 1994, Molecular dissection of ligand binding sites on the low density lipoprotein receptor-related protein, J. Biol. Chem, 269, 15827, 10.1016/S0021-9258(17)40755-1 · DOI
  32. Zagursky, 1995, Quantitation of cellular receptors by a new immunocytochemical flow cytometry technique, Biotechniques, 18, 504
  33. Zheng, 1994, Organ distribution in rats of two members of the low-density lipoprotein receptor gene family, gp330 and LRP/alpa 2MR, and the receptor-associated protein (RAP), J. Histochem. Cytochem, 42, 531, 10.1177/42.4.7510321 · DOI

Cited by

Sorted by citations

Metadata

Authors
Obermoeller-McCormick, L. M., Li, Y., Osaka, H., FitzGerald, D. J., Schwartz, A. L., & Bu, G.
Affiliations
  • Washington University School of Medicine 1 Departments of Pediatrics, St Louis Children’s Hospital , , St Louis, MO 63110, USA
  • 2 Laboratory of Molecular Biology, National Cancer Institute, Bethesda, MD 20892, USA
Venue
Journal of Cell Science
Publisher
The Company of Biologists
Published
2001-03-01
Volume
114
Issue
5
Pages
899-908
ISSN
  • 0021-9533
  • 1477-9137
DOI
10.1242/jcs.114.5.899
Cited by
110
References
1
Language
en
Licence
www.biologists.com/user-licence-1-1/ (vor)

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review