Cited by 197Histone Deacetylase Inhibitors ResearchGenomics and Chromatin DynamicsEpigenetics and DNA Methylation

Isolation and Characterization of Mammalian HDAC10, a Novel Histone Deacetylase

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Kao, Hung-Ying, Lee, Chih-Hao, Komarov, Andrei, Han, Chris C., Evans, Ronald M.·2002·Journal of Biological Chemistry

Abstract

Acetylation of histone core particles plays an important role in modulating chromatin structure and gene expression. The acetylation status of the histone tails is determined by two opposing enzymatic activities, histone acetyltransferases and histone deacetylases (HDACs). Here we describe the isolation and characterization of HDAC10, a novel class II histone deacetylase. Molecular cloning and Northern blot analyses reveal that the HDAC10 transcript is widely expressed and subjected to alternative splicing. HDAC10 is both nuclear and cytoplasmic, a feature reminiscent of HDACs 4, 5, and 7. Distinct from other family members, HDAC10 harbors an amino-terminal catalytic domain and a carboxyl pseudo-repeat that shares significant homology with its catalytic domain. Mutational analysis reveals that transcriptional repression by HDAC10 requires its intrinsic histone deacetylase activity. Taken together, HDAC10 represents a distinct HDAC that may play a role in transcription regulation.

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Crossref · 10.1074/jbc.m108931200

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Authors
Kao, H.-Y., Lee, C.-H., Komarov, A., Han, C. C., & Evans, R. M.
Affiliations
  • University Hospitals of Cleveland (US)
  • Salk Institute for Biological Studies (US)
Venue
Journal of Biological Chemistry
Publisher
Elsevier BV
Published
2002-01
Volume
277
Issue
1
Pages
187-193
ISSN
0021-9258 (ISSN-L)
OpenAlex
W1996507777
Cited by
193
References
53
Citations by year
2026: 5 · 2025: 3 · 2024: 5 · 2023: 12 · 2022: 4 · 2021: 10 · 2020: 13 · 2019: 9 · 2018: 5 · 2017: 4 · 2016: 6 · 2015: 8 · 2014: 10 · 2013: 7 · 2012: 7
FWCI
5.22
Type
journal article
Language
en
Open access
Locations
  • Journal of Biological Chemistry (publishedVersion, cc-by)
  • PubMed (publishedVersion)
Funders
Eunice Kennedy Shriver National Institute of Child Health and Human Development (R01HD027183, HD-27183)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Histone Deacetylase Inhibitors Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Epigenetics and DNA Methylation
Keywords
HDAC10, histone deacetylase, class II HDACs, histone acetylation, chromatin structure, transcriptional repression, alternative splicing, nuclear-cytoplasmic localization, catalytic domain, gene expression
MeSH terms
Amino Acid Sequence, Animals, Base Sequence, Cell Nucleus, Cytoplasm, Histone Deacetylases, Humans, Molecular Sequence Data, Transcription, Genetic, DNA, Complementary, Mice

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