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In vitro study of Prebiotic Properties of Levan-type Exopolysaccharides from Lactobacilli and Non-digestible Carbohydrates Using Denaturing Gradient Gel Electrophoresis
Abstract
Batch cultures inoculated with human faeces were used to study the prebiotic properties of levan-type exopolysaccharides (EPS) from Lactobacillus sanfranciscensis as well as levan, inulin, and fructooligosaccharide (FOS). Denaturing gradient gel electrophoresis of 16S rDNA fragments generated by PCR with universal primers was used to analyse the cultures. Characteristic changes were revealed in the composition of the gut bacteria during fermentation of the carbohydrates. An enrichment of Bifidobacterium spp. was found for the EPS and inulin but not for levan and FOS. The bifidogenic effect of the EPS was confirmed by culturing on selective medium. In addition, the use of EPS and FOS resulted in enhanced growth of Eubacterium biforme and Clostridium perfringens, respectively.
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“A similar bifidogenic effect has been also reported in a previous study examining the prebiotic effect of levan EPS from Fructilactobacillus (Fr.) sanfranciscensis [160].”
From Lactic Acid Bacteria Exopolysaccharides Producers: A Sustainable Tool for Functional Foods (2021) · cites this paper as [160]
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Metadata
- Authors
- Bello, F. D., Walter, J., Hertel, C., & Hammes, W. P.
- Affiliations
- University of Hohenheim (DE)
- Venue
- Systematic and Applied Microbiology
- Publisher
- Elsevier BV
- Published
- 2001
- Volume
- 24
- Issue
- 2
- Pages
- 232-237
- ISSN
- 0723-2020 (ISSN-L)
- OpenAlex
- W2081966773
- Cited by
- 214
- References
- 30
- Citations by year
- 2026: 1 · 2025: 8 · 2024: 4 · 2023: 12 · 2022: 10 · 2021: 14 · 2020: 14 · 2019: 13 · 2018: 14 · 2017: 8 · 2016: 4 · 2015: 16 · 2014: 12 · 2013: 9 · 2012: 12
- FWCI
- 3.65
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Systematic and Applied Microbiology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Nursing › Nutrition and Dietetics › Microbial Metabolites in Food Biotechnology
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Health Sciences › Nursing › Nutrition and Dietetics › Infant Nutrition and Health
- Keywords
- prebiotics, levan, exopolysaccharides, Lactobacillus sanfranciscensis, non-digestible carbohydrates, inulin, fructooligosaccharides, gut microbiota, Bifidobacterium, bifidogenic effect, fecal fermentation, 16S rRNA gene, Clostridium perfringens
- MeSH terms
- Culture Media, Electrophoresis, Agar Gel, Fermentation, Fructans, Humans, Intestines, Inulin, Lactobacillus, Oligosaccharides, Polysaccharides, RNA, Ribosomal, 16S, Colony Count, Microbial, Polymerase Chain Reaction, Probiotics
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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