Adhesion of Histoplasma capsulatum to pneumocytes and biofilm formation on an abiotic surface
Open accessAbstract
The pathogenic fungus, Histoplasma capsulatum, causes the respiratory and systemic disease 'histoplasmosis'. This disease is primarily acquired via inhalation of aerosolized microconidia or hyphal fragments of H. capsulatum. Evolution of this respiratory disease depends on the ability of H. capsulatum yeasts to survive and replicate within alveolar macrophages. It is known that adhesion to host cells is the first step in colonization and biofilm formation. Some microorganisms become attached to biological and non-biological surfaces due to the formation of biofilms. Based on the importance of biofilms and their persistence on host tissues and cell surfaces, the present study was designed to investigate biofilm formation by H. capsulatum yeasts, as well as their ability to adhere to pneumocyte cells. H. capsulatum biofilm assays were performed in vitro using two different clinical strains of the fungus and biofilms were characterized using scanning electron microscopy. The biofilms were measured using a 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino)carbonyl]-2H-tetrazolium-hydroxide (XTT) reduction assay. The results showed that both the H. capsulatum strains tested were very efficient at adhering to host cells and forming biofilm. Therefore, this is a possible survival strategy adopted by this fungus.
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Histoplasma capsulatum may exist in biofilms in vivo.
“suggesting that H. capsulatum may exist in biofilms in vivo (154).”
From <i>Candida albicans</i> Biofilms and Human Disease (2015) · cites this paper as [154]
Histoplasma capsulatum has been reported to form biofilms consisting of dense clusters of yeast-form cells in vitro.
“There is at least one report of H. capsulatum forming biofilms consisting of dense clusters of yeast-form cells in vitro”
From <i>Candida albicans</i> Biofilms and Human Disease (2015) · cites this paper as [154]
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Cites
Principles and Practice of Infectious Diseases.
(1991) · Annals of Internal Medicine · doi:10.7326/0003-4819-114-9-818_5 · Cited by 12,045 · see citing works
Biofilms: Survival Mechanisms of Clinically Relevant Microorganisms
Donlan, R. M., & Costerton, J. W. (2002) · Clinical Microbiology Reviews · doi:10.1128/cmr.15.2.167-193.2002 · Cited by 6,644 · see citing works
Bacterial Biofilms in Nature and Disease
Costerton, J. W., Cheng, K. J., Geesey, G. G., Ladd, T. I., Nickel, J. C., Dasgupta, M., & Marrie, T. J. (1987) · Annual Review of Microbiology · doi:10.1146/annurev.mi.41.100187.002251 · Cited by 3,145 · see citing works
Principles and practice of infectious diseases
Zuckerman, A. J. (2010) · Travel Medicine and Infectious Disease · doi:10.1016/j.tmaid.2010.07.004 · Cited by 2,008 · see citing works
Chandra, J., Kuhn, D. M., Mukherjee, P. K., Hoyer, L. L., McCormick, T., & Ghannoum, M. A. (2001) · Journal of Bacteriology · doi:10.1128/jb.183.18.5385-5394.2001 · Cited by 1,578 · see citing works
Comparison of multiple methods for quantification of microbial biofilms grown in microtiter plates
Peeters, E., Nelis, H. J. C. F., & Coenye, T. (2007) · Journal of Microbiological Methods · doi:10.1016/j.mimet.2007.11.010 · Cited by 1,148 · see citing works
Pierce, C. G., Uppuluri, P., Tristan, A. R., Wormley, F. L., Mowat, E., Ramage, G., & Lopez-Ribot, J. L. (2008) · Nature Protocols · doi:10.1038/nprot.2008.141 · Cited by 1,041 · see citing works
Cell Wall and Secreted Proteins of<i>Candida albicans</i>: Identification, Function, and Expression
Chaffin, W. L., López-Ribot, J. L., Casanova, M., Gozalbo, D., & Martínez, J. P. (1998) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.62.1.130-180.1998 · Cited by 726 · see citing works
Flocculation, adhesion and biofilm formation in yeasts
Verstrepen, K. J., & Klis, F. M. (2006) · Molecular Microbiology · doi:10.1111/j.1365-2958.2006.05072.x · Cited by 589 · see citing works
Our Current Understanding of Fungal Biofilms
Ramage, G., Mowat, E., Jones, B., Williams, C., & Lopez-Ribot, J. (2009) · Critical Reviews in Microbiology · doi:10.3109/10408410903241436 · Cited by 483 · see citing works
Baillie, G. S. (2000) · Journal of Antimicrobial Chemotherapy · doi:10.1093/jac/46.3.397 · Cited by 389 · see citing works
Cystic fibrosis pathogenesis and the role of biofilms in persistent infection
Costerton, J. W. (2001) · Trends in Microbiology · doi:10.1016/s0966-842x(00)01918-1 · Cited by 329 · see citing works
RNA interference in Histoplasma capsulatum demonstrates a role for α‐(1,3)‐glucan in virulence
Rappleye, C. A., Engle, J. T., & Goldman, W. E. (2004) · Molecular Microbiology · doi:10.1111/j.1365-2958.2004.04131.x · Cited by 260 · see citing works
Morris, C. E., Monier, J.-M., & Jacques, M. (1997) · Applied and Environmental Microbiology · doi:10.1128/aem.63.4.1570-1576.1997 · Cited by 241 · see citing works
Martinez, L. R., & Casadevall, A. (2007) · Applied and Environmental Microbiology · doi:10.1128/aem.02506-06 · Cited by 198 · see citing works
Measurement of bacterial adhesion-in vitro evaluation of different methods.
Vesterlund, S., Paltta, J., Karp, M., & Ouwehand, A. (2005) · Journal of Microbiological Methods · doi:10.1016/j.mimet.2004.09.013 · Cited by 180 · see citing works
Long, K. H., Gómez, F. J., Morris, R. E., & Newman, S. L. (2003) · The Journal of Immunology · doi:10.4049/jimmunol.170.1.487 · Cited by 170 · see citing works
Nett, J. E., Cain, M. T., Crawford, K., & Andes, D. R. (2011) · Journal of Clinical Microbiology · doi:10.1128/jcm.02273-10 · Cited by 153 · see citing works
Hawser, S., Norris, H. A., Jessup, C. J., & Ghannoum, M. A. (1998) · Journal of Clinical Microbiology · doi:10.1128/jcm.36.5.1450-1452.1998 · Cited by 140 · see citing works
Adherence mechanisms in human pathogenic fungi.
Tronchin, G., Pihet, M., Lopes-Bezerra, L., & Bouchara, J. (2008) · Medical Mycology · doi:10.1080/13693780802206435 · Cited by 129 · see citing works
Pathogenesis II: Fungal responses to host responses: interaction of host cells with fungi
Mendes‐Giannini, M. J. S., Taylor, M. L., Bouchara, J., et al. (2000) · Medical Mycology · doi:10.1080/mmy.38.s1.113.123 · Cited by 109 · see citing works
Self-citationStrain and growth temperature influence Listeria spp. attachment to intact and cut cabbage
Ells, T. C., & Hansen, L. T. (2006) · International Journal of Food Microbiology · doi:10.1016/j.ijfoodmicro.2006.04.033 · Cited by 100 · see citing works
The quality of dialysate: An integrated approach
Lonnemann, G. (2000) · Kidney International · doi:10.1046/j.1523-1755.2000.07614.x · Cited by 92 · see citing works
Identification ofHistoplasma capsulatumTranscripts Induced in Response to Reactive Nitrogen Species
Nittler, M. P., Murray, D. H., Foo, C. K., & Sil, A. (2005) · Molecular Biology of the Cell · doi:10.1091/mbc.e05-05-0434 · Cited by 80 · see citing works
Medoff, G., Maresca, B., Lambowitz, A. M., et al. (1986) · Journal of Clinical Investigation · doi:10.1172/jci112757 · Cited by 79 · see citing works
Zhou, Y., Wang, G., Li, Y., Liu, Y., Song, Y., Zheng, W., Zhang, N., Hu, X., Shikun, Y., & Jia, J. (2012) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.06082-11 · Cited by 72 · see citing works
The role of cytokines and chemokines in Histoplasma capsulatum infection
Kroetz, D. N., & Deepe, G. S. (2011) · Cytokine · doi:10.1016/j.cyto.2011.07.430 · Cited by 68 · see citing works
Evidence of Bacterial Biofilm in Tubing from Hydraulic Pathway ofHemodialysis System
Man, N. K., Degremont, A., Darbord, J., et al. (1998) · Artificial Organs · doi:10.1046/j.1525-1594.1998.06195.x · Cited by 66 · see citing works
Virulence Factors IN Fungi OF Systemic Mycoses
Kurokawa, C. S., Sugizaki, M. F., & Peraçoli, M. T. S. (1998) · Revista do Instituto de Medicina Tropical de São Paulo · doi:10.1590/s0036-46651998000300001 · Cited by 63 · see citing works
Mendes‐Giannini, M. J. S., Hanna, S. A., da Silva, J. L. M., et al. (2004) · Microbes and Infection · doi:10.1016/j.micinf.2004.05.005 · Cited by 62 · see citing works
Self-citationUsing biofilms as initial inocula in animal models of biofilm‐related infections
Williams, D. L., & Costerton, J. W. (2011) · Journal of Biomedical Materials Research Part B Applied Biomaterials · doi:10.1002/jbm.b.31979 · Cited by 62 · see citing works
Ferreira, M. S., & Borges, A. S. (2009) · Revista da Sociedade Brasileira de Medicina Tropical · doi:10.1590/s0037-86822009000200020 · Cited by 56 · see citing works
Oropharyngeal histoplasmosis: report of eleven cases and review of the literature.
Antonello, V. S., Zaltron, V. F., Vial, M., Oliveira, F. M. D., & Severo, L. C. (2011) · Revista da Sociedade Brasileira de Medicina Tropical · doi:10.1590/s0037-86822011000100007 · Cited by 55 · see citing works
Rooney, P. J., Sullivan, T. D., & Klein, B. S. (2001) · Molecular Microbiology · doi:10.1046/j.1365-2958.2001.02300.x · Cited by 49 · see citing works
Biofilms Invade Nephrology: Effects in Hemodialysis
Cappelli, G., Ballestri, M., Perrone, S., et al. (2000) · Blood Purification · doi:10.1159/000014421 · Cited by 49 · see citing works
Taylor, M. L., RuÃz-Palacios, G. M., Reyes-Montes, M. R., et al. (2005) · FEMS Immunology & Medical Microbiology · doi:10.1016/j.femsim.2005.05.017 · Cited by 45 · see citing works
Self-citationExpression and Interstrain Variability of the YPS3 Gene of Histoplasma capsulatum
Bohse, M. L., & Woods, J. P. (2007) · Eukaryotic Cell · doi:10.1128/ec.00010-07 · Cited by 36 · see citing works
Isolation of Histoplasma capsulatum from bats in the urban area of São Paulo State, Brazil
Dias, M. A. F. S., Zancopé‐Oliveira, R. M., Giudice, M. C., et al. (2010) · Epidemiology and Infection · doi:10.1017/s095026881000289x · Cited by 33 · see citing works
Localization of a yeast-phase-specific gene product to the cell wall in Histoplasma capsulatum
Weaver, C. H., Sheehan, K. C. F., & Keath, E. J. (1996) · Infection and Immunity · doi:10.1128/iai.64.8.3048-3054.1996 · Cited by 28 · see citing works
Genetic diversity ofHistoplasma capsulatumstrains in Brazil
Zancopé-Oliveira, R. M., Tavares, P. M. E. S., & Muniz, M. D. M. (2005) · FEMS Immunology & Medical Microbiology · doi:10.1016/j.femsim.2005.05.018 · Cited by 28 · see citing works
Molecular characterization of Histoplasma capsulatum isolated from an outbreak in treasure hunters
Muñoz, B., Martínez, M. Á., Palma, G., et al. (2010) · BMC Infectious Diseases · doi:10.1186/1471-2334-10-264 · Cited by 25 · see citing works
Biological and sociocultural approaches of histoplasmosis in the State of Guerrero, Mexico
Taylor, M. L., Granados, J., & Toriello, C. (1996) · Mycoses · doi:10.1111/j.1439-0507.1996.tb00157.x · Cited by 24 · see citing works
Self-citationGorocica, P., Taylor, M. L., Alvarado‐Vásquez, N., et al. (2009) · Memórias do Instituto Oswaldo Cruz · doi:10.1590/s0074-02762009000300016 · Cited by 22 · see citing works
Self-citationHistoplasmacapsulatumyeast cells attach and agglutinate human erythrocytes
Taylor, M. L., Duarte-Escalante, E., Pérez, A., et al. (2004) · Medical Mycology · doi:10.1080/13693780310001644734 · Cited by 20 · see citing works
Self-citationDuarte-Escalante, E., Zenteno, E., & Taylor, M. L. (2003) · Archives of Medical Research · doi:10.1016/s0188-4409(03)00044-4 · Cited by 17 · see citing works
Self-citationDisseminated Histoplasmosis in a Patient with Advanced HIV Disease - Lessons Learnt from Bangladesh
Pervez, M. M., Cobb, B. A., Matin, N., et al. (2010) · Journal of Health Population and Nutrition · doi:10.3329/jhpn.v28i3.5561 · Cited by 16 · see citing works
Adherence Patterns of Histoplasma capsulatum Yeasts to Bat Tissue Sections
Suárez-Alvarez, R. O., Pérez‐Torres, A., & Taylor, M. L. (2010) · Mycopathologia · doi:10.1007/s11046-010-9302-9 · Cited by 14 · see citing works
Self-citationTaylor, M. L., Duarte-Escalante, E., Reyes-Montes, M. R., et al. (1998) · Clinical & Experimental Immunology · doi:10.1046/j.1365-2249.1998.00656.x · Cited by 14 · see citing works
Self-citationDimorfismo y patogenia de Histoplasma capsulatum
López, C. (2006) · Redalyc (Universidad Autónoma del Estado de México) · Cited by 12 · see citing works
[Dimorphism and pathogenesis of Histoplasma capsulatum].
López, C. E. (2007) · PubMed · Cited by 9 · see citing works
Biofilm on Artificial Surfaces
Cappelli, G., Ricardi, M., Ravera, F., et al. (2006) · KARGER eBooks · doi:10.1159/000096814 · Cited by 8 · see citing works
PODWALL, YADOR, MILLER, Peña, FRANZOT, LIPETZ, CASADEVALL, & STEINBERG (1998) · Medical Mycology · doi:10.1046/j.1365-280x.1998.00102.x · Cited by 8 · see citing works
Second Case of Histoplasmosis in a Captive Mara (Dolichotis patagonum): Pathological Findings
Pérez‐Torres, A., Rosas-Rosas, A. G., Parás-García, A., et al. (2009) · Mycopathologia · doi:10.1007/s11046-009-9199-3 · Cited by 6 · see citing works
Podwall, D., Yador, E., Miller, S., Pena, J., Franzot, S. P., Lipetz, J., Casadevall, A., & Steinberg, J. J. (1998) · Medical Mycology · doi:10.1080/02681219880000011 · Cited by 6 · see citing works
Italian contributions to the history of general and medical mycology.
Ajello, L. (1998) · PubMed · Cited by 5 · see citing works
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Antifungal Therapy: New Advances in the Understanding and Treatment of Mycosis
Scorzoni, L., de Paula e Silva, A. C. A., Marcos, C. M., Assato, P. A., de Melo, W. C. M. A., de Oliveira, H. C., Costa-Orlandi, C. B., Mendes-Giannini, M. J. S., & Fusco-Almeida, A. M. (2017) · Frontiers in Microbiology · doi:10.3389/fmicb.2017.00036 · Cited by 418 · see citing works
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- Authors
- Pitangui, N. S., Sardi, J. C. O., Silva, J. F., Benaducci, T., Moraes da Silva, R. A., Rodríguez-Arellanes, G., Taylor, M. L., Mendes-Giannini, M. J. S., & Fusco-Almeida, A. M.
- Affiliations
- a Department of Clinical Analysis, Laboratory of Clinical Mycology, Faculty of Pharmaceutical Sciences , UNESP – Univ Estadual Paulista , Araraquara , SP 14801-902 , Brazil
- Universidade Estadual Paulista (Unesp) (BR)
- Universidad Nacional Autónoma de México (MX)
- Venue
- Biofouling
- Publisher
- Informa UK Limited
- Published
- 2012-07-12
- Volume
- 28
- Issue
- 7
- Pages
- 711-718
- ISSN
- 0892-7014 (ISSN-L)
- 1029-2454
- OpenAlex
- W1986557370
- Cited by
- 69
- References
- 55
- Citations by year
- 2026: 1 · 2025: 5 · 2024: 2 · 2023: 7 · 2022: 4 · 2021: 7 · 2020: 6 · 2019: 4 · 2018: 3 · 2017: 7 · 2016: 7 · 2015: 8 · 2014: 3 · 2013: 4
- FWCI
- 3.58
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Biofouling (publishedVersion)
- PubMed (publishedVersion)
- Acervo Digital da Universidade Estadual Paulista (Universidade Estadual Paulista) (submittedVersion)
- UNESP Institutional Repository (São Paulo State University) (submittedVersion)
- Funders
- Universidade de São Paulo
- Dirección General de Asuntos del Personal Académico, Universidad Nacional Autónoma de México
- Topics
- Health Sciences › Medicine › Epidemiology › Fungal Infections and Studies
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
- Keywords
- Histoplasma capsulatum, histoplasmosis, fungal adhesion, alveolar epithelial cells, biofilm formation, abiotic surfaces, host cell attachment, alveolar macrophages
- MeSH terms
- Cell Adhesion, Cell Line, Histoplasma, Histoplasmosis, Humans, Microscopy, Electron, Scanning, Tetrazolium Salts, Biofilms, Host-Pathogen Interactions, Alveolar Epithelial Cells
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