Molecular heterogeneity in Aphanizomenon flos-aquae and Anabaena flos-aquae(Cyanophyta): a synchrotron-based Fourier-transform infrared study of lake micropopulations
Open accessAbstract
Synchrotron-based Fourier-transform infrared (FTIR) microspectroscopy was used to characterize micropopulations of the cyanobacteria Anabaena flos-aquae and Aphanizomenon flos-aquae in mixed phytoplankton samples taken from the epilimnion and hypolimnion of a stratified eutrophic lake (Rostherne Mere, UK). All FTIR spectra (normalized to amide I) showed a closely similar sequence of 10 bands over the wavenumber range 4,000–900 cm−1 and were assigned to a range of vibrationally active chemical groups. Interspecific differences between Anabaena and Aphanizomenon were revealed using correlation and multivariate analysis (principal component analysis, PCA) of the set of individual band intensities. Differences between species (separately for epilimnion and hypolimnion) were also analysed by comparing band intensity means, and by performing PCA over a broad spectral region (1,750–900 cm−1). Comparison of intensity means showed that spectra obtained from epilimnetic algae had significant differences in bands 5–7 and in bands 4, 9 and 10. However, in the spectra obtained from the hypolimnion algae, a different pattern was observed, with significant differences between the species restricted to bands 7–10. PCA of the spectral region 1,750–900 cm−1 also demonstrated interspecific differences in relation to carbohydrate and protein concentrations, with Anabaena displaying higher carbohydrate levels in both the epilimnion and hypolimnion. Intraspecific comparisons of band intensity means for epilimnion and hypolimnion subpopulations demonstrated clear differences in Aphanizomenon, where significant differences were observed between epilimnion (higher carbohydrate concentration compared with protein) and hypolimnion (lower carbohydrate concentration compared with protein) subpopulations. Anabaena however showed only minor differences. PCA comparison of the spectral region 1,750–900 cm−1, followed by data visualization using scores plots and loading plots, also demonstrated quantitative differences. Scores plots resolved the epilimnion and hypolimnion subpopulations in Aphanizomenon (but not Anabaena), with the loading plot showing large differences in carbohydrates, supporting the analysis using discrete peak intensities.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
FTIR with multivariate analysis has been used successfully to classify species and to differentiate the nutritional status of microalgae.
“The application of FTIR to classify species with the help of multivariate analysis [24 -29] and to differentiate the nutritional status of microalgae [30 -34] have been attempted successfully.”
From Response to nutrient variation on lipid productivity in green microalgae captured using second derivative FTIR and Raman spectroscopy (2022) · cites this paper as [33]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Infrared spectroscopy of biomolecules
Howland, J. L. (1996) · Biochemical Education · doi:10.1016/0307-4412(96)82531-3 · Cited by 799 · see citing works
Hill, W. R. (1996) · Elsevier eBooks · doi:10.1016/b978-012668450-6/50034-5 · Cited by 425 · see citing works
Wong, P. T., Wong, R. K. S., Caputo, T. A., Godwin, T. A., & Rigas, B. (1991) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.88.24.10988 · Cited by 353 · see citing works
Biological applications of infrared spectroscopy
Stuart, B. H., & Ando, D. (1997) · Medical Entomology and Zoology · Cited by 343 · see citing works
A dynamic model of photoadaptation in phytoplankton
Geider, R. J., MacIntyre, H. L., & Kana, T. M. (1996) · Limnology and Oceanography · doi:10.4319/lo.1996.41.1.0001 · Cited by 320 · see citing works
Giordano, M., Kansiz, M., Heraud, P., Beardall, J., Wood, B., & McNaughton, D. (2001) · Journal of Phycology · doi:10.1046/j.1529-8817.2001.037002271.x · Cited by 301 · see citing works
Holman, H. N., Martin, M. C., Blakely, E. A., Bjornstad, K. A., & McKinney, W. R. (2000) · Biopolymers · doi:10.1002/1097-0282(2000)57:6 · Cited by 245 · see citing works
Kansiz, M., Heraud, P., Wood, B., Burden, F., Beardall, J., & McNaughton, D. (1999) · Phytochemistry · doi:10.1016/s0031-9422(99)00212-5 · Cited by 238 · see citing works
Benning, L. G., Phoenix, V. R., Yee, N., & Tobin, M. J. (2004) · Geochimica et Cosmochimica Acta · doi:10.1016/s0016-7037(03)00489-7 · Cited by 192 · see citing works
Ultraspatially-resolved synchrotron infrared microspectroscopy of plant tissue in situ.
Wetzel, D. L., Aj, E., Pietrzak, L. N., Miller, S. S., & Ja, S. (1998) · PubMed · Cited by 168 · see citing works
Guibal, E., Roulph, C., & Cloirec, P. L. (1995) · Environmental Science & Technology · doi:10.1021/es00010a007 · Cited by 116 · see citing works
Infrared Spectroscopy of Nucleic Acids
Gp, Z., & Oleinik, E. F. (1972) · Russian Chemical Reviews · doi:10.1070/rc1972v041n03abeh002043 · Cited by 83 · see citing works
INTERNAL NITRATE CONCENTRATIONS IN SINGLE CELLS OF LARGE PHYTOPLANKTON FROM THE SARGASSO SEA1
Villareal, T. A., & Lipschultz, F. (1995) · Journal of Phycology · doi:10.1111/j.0022-3646.1995.00689.x · Cited by 82 · see citing works
The Sigma Library of FT-IR Spectra
Keller, R. J. (2007) · Medical Entomology and Zoology · Cited by 78 · see citing works
SIGEE, D. C., DEAN, A., LEVADO, E., & TOBIN, M. J. (2002) · European Journal of Phycology · doi:10.1017/s0967026201003444 · Cited by 72 · cited 1× · see citing works
“Although FTIR microspectroscopy has largely been used in relation to purified biochemicals such as nucleic acids (Liquier & Taillandier, 1996 ), proteins (Stuart, 1997 ), lipids (Lewis & McElhaney, 1996 ) and polysaccharides (Brandenberg & Seydel, 1996 ), it may also be applied to more complex biological samples – inc…”
MethodSelf-citationMarañón, E., & González, N. (1997) · Marine Ecology Progress Series · doi:10.3354/meps157061 · Cited by 42 · see citing works
El‐Bestawy, E., Bellinger, E. G., & Sigee, D. C. (1996) · European Journal of Phycology · doi:10.1080/09670269600651331 · Cited by 26 · see citing works
Self-citationSigee, D. C. (2000) · Journal of Plankton Research · doi:10.1093/plankt/22.11.2137 · Cited by 25 · see citing works
Self-citationEmilio Marañón, C., Fernández, E., & Anadón, R. (1995) · Journal of Experimental Marine Biology and Ecology · doi:10.1016/0022-0981(94)00177-f · Cited by 21 · see citing works
FOURIER TRANSFORM INFRARED SPECTROSCOPY: A MOLECULAR APPROACH TO AN ORGANISMAL QUESTION
Sacksteder, C. A., & Barry, B. A. (2001) · Journal of Phycology · doi:10.1046/j.1529-8817.2001.037002197.x · Cited by 18 · see citing works
Sigee, D., Levado, E., & Dodwell, A. (1999) · Aquatic Microbial Ecology · doi:10.3354/ame019177 · Cited by 17 · see citing works
Self-citationSigee, D. C., TEPER, J., & Levado, E. (1999) · European Journal of Phycology · doi:10.1080/09541449910001718831 · Cited by 13 · see citing works
Self-citationPhysiological change in microalgae monitored using FT-IR spectroscopy
Heraud, P., Wood, B. R., Beardall, J., & McNaughton, D. (1999) · Springer eBooks · doi:10.1007/978-94-011-4479-7_201 · Cited by 1 · see citing works
Flagged first, then by citations
Research works printed but not linked: 3
- LEE, R. (1997). Phycology. Cambridge University Press, Cambridge.
- LEWIS, R.N. & MCELHANEY, R.N. (1996). Fourier transform infrared spectroscopy in the study of hydrated lipids and lipid bilayer membranes. In Infrared Spectroscopy of Biomolecules (Mantsch, H.H. and Chapman, D., editors), 159-202. Wiley, Chichester.
- NAUMANN, D., SCHULTZ, C.P. & HELM, D. (1996). What can infrared spectroscopy tell us about the structure and composition of intact bacterial cells? In Infrared Spectroscopy of Biomolecules (Mantsch, H.H. and Chapman, D., editors), 279-310. Wiley, Chichester.
Cited by
Sorted by citations
FT-IR Microspectroscopy Enhances Biological and Ecological Analysis of Algae
Murdock, J. N., & Wetzel, D. L. (2009) · Applied Spectroscopy Reviews · doi:10.1080/05704920902907440 · Cited by 223 · see citing works
Dilek (Yalcin) Duygu (2012) · AFRICAN JOURNAL OF BIOTECHNOLOGY · doi:10.5897/ajb11.1863 · Cited by 146 · see citing works
Osundeko, O., Dean, A. P., Davies, H. S., & Pittman, J. K. (2014) · Plant and Cell Physiology · doi:10.1093/pcp/pcu113 · Cited by 133 · see citing works
Self-citationBajhaiya, A. K., Dean, A. P., Zeef, L. A. H., Webster, R. E., & Pittman, J. K. (2015) · PLANT PHYSIOLOGY · doi:10.1104/pp.15.01907 · Cited by 133 · see citing works
Self-citationDetailed Characterization of the Cell Wall Structure and Composition of Nordic Green Microalgae
Spain, O., & Funk, C. (2022) · Journal of Agricultural and Food Chemistry · doi:10.1021/acs.jafc.2c02783 · Cited by 127 · see citing works
Padedda, B. M., Sechi, N., Lai, G. G., Mariani, M. A., Pulina, S., Sarria, M., Satta, C. T., Virdis, T., Buscarinu, P., & Lugliè, A. (2017) · Global Ecology and Conservation · doi:10.1016/j.gecco.2017.08.004 · Cited by 110 · see citing works
Dean, A. P., Nicholson, J. M., & Sigee, D. C. (2008) · European Journal of Phycology · doi:10.1080/09670260801979287 · Cited by 81 · see citing works
Self-citationDomenighini, A., & Giordano, M. (2009) · Journal of Phycology · doi:10.1111/j.1529-8817.2009.00662.x · Cited by 78 · see citing works
Kashyap, M., Samadhiya, K., Ghosh, A., Anand, V., Shirage, P. M., & Bala, K. (2019) · RSC Advances · doi:10.1039/c9ra04451e · Cited by 67 · see citing works
Landeta-Salgado, C., Muñoz, R., Blanco, A., & Lienqueo, M. E. (2020) · Algal Research · doi:10.1016/j.algal.2020.102135 · Cited by 64 · see citing works
Sigee, D. C., Bahrami, F., Estrada, B., Webster, R. E., & Dean, A. P. (2007) · Phycologia · doi:10.2216/07-14.1 · Cited by 64 · see citing works
Self-citationDean, A. P., Martin, M. C., & Sigee, D. C. (2007) · Phycologia · doi:10.2216/06-27.1 · Cited by 58 · see citing works
Self-citationGebru, M. G., Mishra, S., & Mohanty, K. (2018) · Journal of environmental chemical engineering · doi:10.1016/j.jece.2018.08.061 · Cited by 51 · see citing works
Beratto-Ramos, A., Agurto-Muñoz, C., Vargas-Montalba, J. P., & Castillo, R. D. P. (2019) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2019.115561 · Cited by 45 · see citing works
Talluri, S. N. L., Winter, R. M., & Salem, D. R. (2020) · Biofouling · doi:10.1080/08927014.2020.1748186 · Cited by 43 · see citing works
Landeta-Salgado, C., Cicatiello, P., & Lienqueo, M. E. (2021) · Algal Research · doi:10.1016/j.algal.2021.102314 · Cited by 44 · see citing works
Evaluation of biochemical algal floc properties using Reflectance Fourier-Transform Infrared Imaging
González-Torres, A., Rich, A. M., Marjo, C. E., & Henderson, R. K. (2017) · Algal Research · doi:10.1016/j.algal.2017.09.017 · Cited by 37 · see citing works
Dean, A. P., Estrada, B., Nicholson, J. M., & Sigee, D. C. (2008) · Phycological Research · doi:10.1111/j.1440-1835.2008.00501.x · Cited by 32 · see citing works
Self-citationKashyap, M., Samadhiya, K., Ghosh, A., Anand, V., Lee, H., Sawamoto, N., Ogura, A., Ohshita, Y., Shirage, P. M., & Bala, K. (2021) · Environmental Research · doi:10.1016/j.envres.2021.111499 · Cited by 31 · see citing works
Cyanotoxin cylindrospermopsin producers and the catalytic decomposition process: A review.
Adamski, M., Wolowski, K. M., Kamiński, A., & Hindáková, A. (2020) · Harmful Algae · doi:10.1016/j.hal.2020.101894 · Cited by 28 · see citing works
A Novel Adaptation Mechanism Underpinning Algal Colonization of a Nuclear Fuel Storage Pond
MeGraw, V. E., Brown, A. R., Boothman, C., Goodacre, R., Morris, K., Sigee, D. C., Anderson, L., & Lloyd, J. R. (2018) · mBio · doi:10.1128/mbio.02395-17 · Cited by 25 · see citing works
Self-citationEsther Elizabeth Grace, C., Briget Mary, M., Vaidyanathan, S., & Srisudha, S. (2022) · Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy · doi:10.1016/j.saa.2021.120830 · Cited by 21 · see citing works
Why productive lakes are larger mercury sedimentary sinks than oligotrophic brown water lakes
Schütze, M., Gatz, P., Gilfedder, B. S., & Biester, H. (2020) · Limnology and Oceanography · doi:10.1002/lno.11684 · Cited by 21 · see citing works
Agnihotri, V. K. (2013) · Critical Reviews in Environmental Science and Technology · doi:10.1080/10643389.2013.803797 · Cited by 21 · see citing works
Variation in gametophyte dominance in populations of Chondrus verrucosus (Gigartinaceae, Rhodophyta)
Bellgrove, A., & Aoki, M. N. (2008) · Phycological Research · doi:10.1111/j.1440-1835.2008.00506.x · Cited by 19 · see citing works
Macromolecular Response of Individual Algal Cells to Nutrient and Atrazine Mixtures within Biofilms
Murdock, J. N., & Wetzel, D. L. (2011) · Microbial Ecology · doi:10.1007/s00248-011-9994-5 · Cited by 18 · see citing works
Classification of Toxic Cyanobacterial Blooms by Fourier-Transform Infrared Technology (FTIR)
Kenne, G., & van der Merwe, D. (2013) · Advances in Microbiology · doi:10.4236/aim.2013.36a001 · Cited by 17 · see citing works
Beratto, A., Agurto, C., Freer, J., Peña-Farfal, C., Troncoso, N., Agurto, A., & Castillo, R. D. P. (2017) · Applied Spectroscopy · doi:10.1177/0003702817715654 · Cited by 17 · see citing works
Subcellular localized chemical imaging of benthic algal nutritional content via HgCdTe array FT-IR
Murdock, J. N., Dodds, W. K., & Wetzel, D. L. (2008) · Vibrational Spectroscopy · doi:10.1016/j.vibspec.2007.12.011 · Cited by 17 · see citing works
Fanesi, A., Wagner, H., Birarda, G., Vaccari, L., & Wilhelm, C. (2019) · BMC Plant Biology · doi:10.1186/s12870-019-1736-8 · Cited by 14 · see citing works
Changing patterns of carbon allocation in lake phytoplankton: an FTIR analysis
Dean, A. P., Nicholson, J. M., & Sigee, D. C. (2011) · Hydrobiologia · doi:10.1007/s10750-011-0973-0 · Cited by 15 · see citing works
Self-citationThe Science of Carbon Footprint Assessment
Ramachandra, T. V., & Mahapatra, D. M. (2015) · CRC Press eBooks · doi:10.1201/b18929-3 · Cited by 10 · see citing works
Infrared and Raman Spectroscopic Mapping and Imaging of Plant Materials
Schulz, H., Krähmer, A., Naumann, A., & Gudi, G. (2014) · doi:10.1002/9783527678136.ch6 · Cited by 7 · see citing works
Saini, N., & Mona, S. (2024) · Algal Research · doi:10.1016/j.algal.2024.103757 · Cited by 4 · see citing works
Park, J. M., You, Y., Kang, N. S., Cho, E., Back, C., & Hong, J. W. (2024) · Microbiology and Biotechnology Letters · doi:10.48022/mbl.2408.08003 · Cited by 3 · see citing works
Jarni, K., Bulc, T. G., & Klemenčić, A. K. (2017) · Acta Biologica Slovenica · doi:10.14720/abs.60.1.15666 · Cited by 2 · see citing works
Manikandan, S., Inbakandan, D., Madamuthu, M., Haripraba, M., Clements, C., Thirumurugan, N. K., Anu, C., Kumar, C., & Thirugnanasambandam, R. (2026) · Langmuir · doi:10.1021/acs.langmuir.5c05333 · Cited by 1 · see citing works
Detecting carbon uptake and cellular allocation by individual algae in multispecies assemblages
Murdock, J. N. (2015) · Limnology and Oceanography Methods · doi:10.1002/lom3.10078 · Cited by 1 · see citing works
Kenne, G. (2013) · K-State Research Exchange (Kansas State University)
Velgosová, O., Dolinská, S., & Veselovský, L. (2020) · Molecular Crystals and Liquid Crystals · doi:10.1080/15421406.2020.1840689
Bellinger, E. G., & Sigee, D. C. (2010) · doi:10.1002/9780470689554.refs
Self-citation
- Authors
- Dean, A. P., & Sigee, D. C.
- Affiliations
- University of Manchester (GB)
- Corresponding author
- Sigee, David C.
- ORCID
- Dean, Andrew P.
- Venue
- European Journal of Phycology
- Publisher
- Informa UK Limited
- Published
- 2006-05
- Volume
- 41
- Issue
- 2
- Pages
- 201-212
- ISSN
- 0967-0262 (ISSN-L)
- 1469-4433
- OpenAlex
- W1992762776
- Cited by
- 41
- References
- 23
- Citations by year
- 2026: 1 · 2024: 2 · 2022: 1 · 2021: 3 · 2020: 5 · 2019: 3 · 2018: 2 · 2017: 4 · 2015: 3 · 2014: 2 · 2013: 3 · 2012: 1
- FWCI
- 1.53
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- European Journal of Phycology (publishedVersion)
- Research Explorer (The University of Manchester) (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Spectroscopy Techniques in Biomedical and Chemical Research
- Physical Sciences › Environmental Science › Environmental Chemistry › Aquatic Ecosystems and Phytoplankton Dynamics
- Physical Sciences › Chemistry › Analytical Chemistry › Spectroscopy and Chemometric Analyses
- Keywords
- Anabaena flos-aquae, Aphanizomenon flos-aquae, cyanobacteria, molecular heterogeneity, FTIR microspectroscopy, epilimnion, hypolimnion, lake stratification, interspecific differences, intraspecific variation, carbohydrate content, protein content, eutrophic lakes, phytoplankton
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
