Resolution of codominant phytoplankton species in a eutrophic lake using synchrotron-based Fourier transform infrared spectroscopy
Abstract
A.P. DEAN, M.C. MARTIN AND D.C. SIGEE. 2007. Resolution of codominant phytoplankton species in a eutrophic lake using synchrotron-based Fourier transform infrared spectroscopy. Phycologia 46: 000–000. Synchrotron-based Fourier-transform infrared (FTIR) microspectroscopy was used to distinguish micropopulations of the codominant algae Microcystis aeruginosa (Cyanophyceae) and Ceratium hirundinella (Dinophyceae) in mixed phytoplankton samples taken from the water column of a stratified eutrophic lake (Rostherne Mere, UK). FTIR spectra of the two algae showed a closely similar sequence of 10 bands over the wave-number range 4000–900 cm �1. These were assigned to a range of vibrationally active chemical groups using published band assignments and on the basis of correlation and factor analysis. In both algae, intracellular concentrations of macromolecular components (determined as band intensity) varied considerably within the same population, indicating substantial intraspecific heterogeneity. Interspecific differences were separately analysed in relation to discrete bands and by multivariate analysis of the entire spectral region 1750–900 cm �1. In terms of discrete bands, comparison of individual intensities (normalised to amide 1) demonstrated significant (99% probability level) differences in relation to six bands between the two algal species. Key interspecific differences were also noted in relation to the positions of bands 2, 10 (carbohydrate) and 7 (protein) and in the 3-D plots derived by principal component analysis (PCA) of the sequence of band intensities. PCA of entire spectral regions showed clear resolution of species in the PCA plot, with indication of separation on the basis of protein (region 1700–1500 cm �1) and carbohydrate
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FTIR with multivariate analysis has been used successfully to classify species and to differentiate the nutritional status of microalgae.
“The application of FTIR to classify species with the help of multivariate analysis [24 -29] and to differentiate the nutritional status of microalgae [30 -34] have been attempted successfully.”
From Response to nutrient variation on lipid productivity in green microalgae captured using second derivative FTIR and Raman spectroscopy (2022) · cites this paper as [30]
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Growth and reproductive strategies of freshwater phytoplankton
(1989) · Choice Reviews Online · doi:10.5860/choice.27-0937 · Cited by 574 · see citing works
Wong, P. T., Wong, R. K. S., Caputo, T. A., Godwin, T. A., & Rigas, B. (1991) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.88.24.10988 · Cited by 353 · see citing works
Mariey, L., Signolle, J. P., Amiel, C., & Travert, J. (2001) · Vibrational Spectroscopy · doi:10.1016/s0924-2031(01)00113-8 · Cited by 345 · see citing works
Biological applications of infrared spectroscopy
Stuart, B. H., & Ando, D. (1997) · Medical Entomology and Zoology · Cited by 343 · see citing works
Stehfest, K., Toepel, J., & Wilhelm, C. (2005) · Plant Physiology and Biochemistry · doi:10.1016/j.plaphy.2005.07.001 · Cited by 316 · see citing works
Giordano, M., Kansiz, M., Heraud, P., Beardall, J., Wood, B., & McNaughton, D. (2001) · Journal of Phycology · doi:10.1046/j.1529-8817.2001.037002271.x · Cited by 301 · see citing works
Holman, H. N., Martin, M. C., Blakely, E. A., Bjornstad, K. A., & McKinney, W. R. (2000) · Biopolymers · doi:10.1002/1097-0282(2000)57:6 · Cited by 245 · see citing works
Kansiz, M., Heraud, P., Wood, B., Burden, F., Beardall, J., & McNaughton, D. (1999) · Phytochemistry · doi:10.1016/s0031-9422(99)00212-5 · Cited by 238 · see citing works
Benning, L. G., Phoenix, V. R., Yee, N., & Tobin, M. J. (2004) · Geochimica et Cosmochimica Acta · doi:10.1016/s0016-7037(03)00489-7 · Cited by 192 · see citing works
Ultraspatially-resolved synchrotron infrared microspectroscopy of plant tissue in situ.
Wetzel, D. L., Aj, E., Pietrzak, L. N., Miller, S. S., & Ja, S. (1998) · PubMed · Cited by 168 · see citing works
A simplified method of phytoplankton counting
Willén, E. (1976) · British Phycological Journal · doi:10.1080/00071617600650551 · Cited by 160 · see citing works
Litaker, R. W., Vandersea, M. W., Kibler, S. R., Reece, K. S., Stokes, N. A., Steidinger, K. A., Millie, D. F., Bendis, B., Pigg, R. J., & Tester, P. A. (2003) · Journal of Phycology · doi:10.1046/j.1529-8817.2003.02112.x · Cited by 138 · see citing works
Heraud, P., Wood, B. R., Tobin, M. J., Beardall, J., & McNaughton, D. (2005) · FEMS Microbiology Letters · doi:10.1016/j.femsle.2005.06.021 · Cited by 123 · see citing works
Guibal, E., Roulph, C., & Cloirec, P. L. (1995) · Environmental Science & Technology · doi:10.1021/es00010a007 · Cited by 116 · see citing works
Enumeration of naturalMicrocystispopulations
REYNOLDS, C. S., & Jaworski, G. H. M. (1978) · British Phycological Journal · doi:10.1080/00071617800650331 · Cited by 114 · see citing works
A comparison of methods for detection of phosphate limitation in microalgae
Beardall, J., Berman, T., Heraud, P., Kadiri, M. O., Light, B., Patterson, G., Roberts, S., Sulzberger, B., Sahan, E., Uehlinger, U., et al. (2001) · Aquatic Sciences · doi:10.1007/pl00001342 · Cited by 100 · see citing works
Infrared Spectroscopy of Nucleic Acids
Gp, Z., & Oleinik, E. F. (1972) · Russian Chemical Reviews · doi:10.1070/rc1972v041n03abeh002043 · Cited by 83 · see citing works
INTERNAL NITRATE CONCENTRATIONS IN SINGLE CELLS OF LARGE PHYTOPLANKTON FROM THE SARGASSO SEA1
Villareal, T. A., & Lipschultz, F. (1995) · Journal of Phycology · doi:10.1111/j.0022-3646.1995.00689.x · Cited by 82 · see citing works
PHOSPHORUS, NITROGEN AND THE GROWTH OF ALGAE IN LAKE KINNERET1
Serruya, C., & Berman, T. (1975) · Journal of Phycology · doi:10.1111/j.1529-8817.1975.tb02764.x · Cited by 78 · see citing works
SIGEE, D. C., DEAN, A., LEVADO, E., & TOBIN, M. J. (2002) · European Journal of Phycology · doi:10.1017/s0967026201003444 · Cited by 72 · see citing works
Dean, A. P., & Sigee, D. C. (2006) · European Journal of Phycology · doi:10.1080/09670260600645907 · Cited by 41 · see citing works
Self-citationSigee, D. C. (2000) · Journal of Plankton Research · doi:10.1093/plankt/22.11.2137 · Cited by 25 · see citing works
FOURIER TRANSFORM INFRARED SPECTROSCOPY: A MOLECULAR APPROACH TO AN ORGANISMAL QUESTION
Sacksteder, C. A., & Barry, B. A. (2001) · Journal of Phycology · doi:10.1046/j.1529-8817.2001.037002197.x · Cited by 18 · see citing works
PHOSPHORUS, NITROGEN AND THE GROWTH OF ALGAE IN LAKE KINNERET1
Serruya, C., & Berman, T. (1975) · Journal of Phycology · doi:10.1111/j.0022-3646.1975.00155.x · Cited by 18 · see citing works
Cellular and Molecular Biology
(2014) · Cellular and Molecular Biology · doi:10.14715/cmb/2013.60.1.2 · Cited by 5 · see citing works
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Murdock, J. N., & Wetzel, D. L. (2009) · Applied Spectroscopy Reviews · doi:10.1080/05704920902907440 · Cited by 223 · see citing works
Dilek (Yalcin) Duygu (2012) · AFRICAN JOURNAL OF BIOTECHNOLOGY · doi:10.5897/ajb11.1863 · Cited by 146 · see citing works
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Dean, A. P., Nicholson, J. M., & Sigee, D. C. (2008) · European Journal of Phycology · doi:10.1080/09670260801979287 · Cited by 81 · see citing works
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Kahzad, N., & Salehzadeh, A. (2020) · Biological Trace Element Research · doi:10.1007/s12011-020-02055-5 · Cited by 76 · see citing works
Wissel, H., Mayr, C., & Lücke, A. (2008) · Organic Geochemistry · doi:10.1016/j.orggeochem.2008.07.014 · Cited by 67 · see citing works
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Poolthajit, S., Takaeh, S., Hahor, W., Nuntapong, N., Ngampongsai, W., & Thongprajukaew, K. (2024) · Animals · doi:10.3390/ani14203028 · Cited by 2 · see citing works
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Bellinger, E. G., & Sigee, D. C. (2015) · doi:10.1002/9781118917152.refs · Cited by 1 · see citing works
Kenne, G. (2013) · K-State Research Exchange (Kansas State University)
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Bellinger, E. G., & Sigee, D. C. (2010) · doi:10.1002/9780470689554.refs
Goff, K. L., Wilson, K. E., & Ellis, T. H. (2025) · Canadian Journal of Chemistry · doi:10.1139/cjc-2024-0237
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- Authors
- Dean, A. P., Martin, M. C., & Sigee, D. C.
- Venue
- Phycologia
- Publisher
- Informa UK Limited
- Published
- 2007-02
- Volume
- 46
- Issue
- 2
- Pages
- 151-159
- ISSN
- 0031-8884 (ISSN-L)
- OpenAlex
- W2136780817
- Cited by
- 58
- References
- 25
- Citations by year
- 2026: 2 · 2025: 6 · 2024: 4 · 2023: 1 · 2022: 4 · 2021: 4 · 2020: 6 · 2019: 4 · 2017: 2 · 2016: 1 · 2015: 7 · 2014: 2 · 2013: 2 · 2012: 3
- FWCI
- 1.55
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Phycologia (publishedVersion)
- Research Explorer (The University of Manchester) (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Spectroscopy Techniques in Biomedical and Chemical Research
- Physical Sciences › Environmental Science › Environmental Chemistry › Aquatic Ecosystems and Phytoplankton Dynamics
- Physical Sciences › Chemistry › Analytical Chemistry › Spectroscopy and Chemometric Analyses
- Keywords
- Microcystis aeruginosa, Ceratium hirundinella, species discrimination, FTIR microspectroscopy, synchrotron infrared spectroscopy, eutrophic lakes, macromolecular composition, protein and carbohydrate content, band assignment
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