Lycium barbarum polysaccharide protects human keratinocytes against UVB-induced photo-damage
Abstract
Ultraviolet B (UVB) irradiation plays a key role in skin damage, which induces oxidative and inflammatory damages, thereby causing photoaging or photocarcinogenesis. Lycium barbarum polysaccharide (LBP), the most biologically active fraction of wolfberry, possesses significant antioxidative and anti-inflammatory effects on multiple tissues. In the present study, the photoprotective effects and potential underlying molecular mechanisms of LBP against UVB-induced photo-damage were investigated in immortalized human keratinocytes (HaCaT cells). The data indicated that pretreatment with LBP significantly attenuated UVB-induced decrease in cell viability, increase in ROS production and DNA damage. LBP also significantly suppressed UVB-induced p38 MAPK activation, and subsequently reversed caspase-3 activation and MMP-9 expression. Notably, LBP was found to induce Nrf2 nuclear translocation and increase the expression of Nrf2-dependent ARE target genes. Furthermore, the protective effects of LBP were abolished by siRNA-mediated Nrf2 silencing. These results showed that the antioxidant LBP could partially protect against UVB irradiation-induced photo-damage through activation of Nrf2/ARE pathway, thereby scavenging ROS and reducing DNA damage, and subsequently suppressing UVB-induced p38 MAP pathway. Thus, LBP can be potentially used for skincare against oxidative damage from environmental insults.
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Oral polysaccharides enhance skin antioxidant enzyme activity, remove ROS, and reduce oxidative damage.
“Mechanistically, oral polysaccharides enhance skin antioxidant enzyme activity, remove ROS, and reduce oxidative damage.”
From Diet and Skin Aging—From the Perspective of Food Nutrition (2020) · cites this paper as [118]
Polysaccharides inhibit collagen degradation by inhibiting the expression of MMP-1 and MMP-9, maintaining a stable collagen ratio, repairing skin structure, and maintaining skin moisture content.
“Finally, polysaccharides inhibit collagen degradation by inhibiting the expression of enzymes such as MMP–1 and MMP–9, maintaining a stable collagen ratio, repairing skin structure, and maintaining skin moisture content [116,117,118,119,120,121].”
From Diet and Skin Aging—From the Perspective of Food Nutrition (2020) · cites this paper as [118]
Oral polysaccharides regulate the expression of Bcl-2, Bax, and Caspase-3 by activating Nrf2/ARE and other pathways, and inhibit apoptosis.
“They regulate the expression of Bcl–2, Bax, and Caspase–3 by activating Nrf2/ARE and other pathways, and inhibit apoptosis.”
From Diet and Skin Aging—From the Perspective of Food Nutrition (2020) · cites this paper as [118]
Polysaccharides such as agaric polysaccharides, Lycium polysaccharides, algal polysaccharides, lingzhi polysaccharides, and mushroom polysaccharides alleviate skin aging.
“Polysaccharides such as agaric polysaccharides, Lycium polysaccharides, algal polysaccharides, lingzhi polysaccharides, and mushroom polysaccharides have been found to alleviate skin aging.”
From Diet and Skin Aging—From the Perspective of Food Nutrition (2020) · cites this paper as [118]
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- Authors
- Li, H., Li, Z., Peng, L., Jiang, N., Liu, Q., Zhang, E., Liang, B., Li, R., & Zhu, H.
- Affiliations
- Guangzhou Institute of Dermatology (CN)
- Guangzhou First People's Hospital (CN)
- Corresponding author
- Zhu, Huilan
- Venue
- Free Radical Research
- Publisher
- Informa UK Limited
- Published
- 2017-02
- Volume
- 51
- Issue
- 2
- Pages
- 200-210
- ISSN
- 1029-2470 (ISSN-L)
- 1071-5762
- OpenAlex
- W2595642624
- Cited by
- 79
- References
- 51
- Citations by year
- 2026: 9 · 2025: 7 · 2024: 14 · 2023: 14 · 2022: 9 · 2021: 8 · 2020: 5 · 2019: 5 · 2018: 6 · 2017: 2
- FWCI
- 5.59
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Free Radical Research (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Building Powerful Province of Traditional Medicine Project of Guangdong, China (20141221)
- Natural Science Foundation of Guangdong Province, China (2015A030313765)
- Science and Technology Project of Guangzhou, China (201604020093)
- Topics
- Health Sciences › Medicine › Dermatology › Skin Protection and Aging
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics, phytochemicals, and oxidative stress
- Health Sciences › Medicine › Biochemistry › Phytochemicals and Antioxidant Activities
- Keywords
- Lycium barbarum polysaccharide, keratinocytes, photoprotection, Nrf2/ARE pathway, p38 MAPK, caspase-3, MMP-9, reactive oxygen species, DNA damage, oxidative stress, anti-inflammatory activity, photoaging, skin care
- MeSH terms
- Antioxidants, Cells, Cultured, DNA Damage, Drugs, Chinese Herbal, Humans, Radiation-Protective Agents, RNA, Messenger, Ultraviolet Rays, Blotting, Western, Signal Transduction, Keratinocytes, Apoptosis, Reactive Oxygen Species, Oxidative Stress, Reverse Transcriptase Polymerase Chain Reaction, RNA, Small Interfering, Cell Proliferation, NF-E2-Related Factor 2, Real-Time Polymerase Chain Reaction
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