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Pregnancy-related changes in the maternal gut microbiota are dependent upon the mother's periconceptional diet
Open accessAbstract
Shifts in the maternal gut microbiome have been implicated in metabolic adaptations to pregnancy. We investigated how pregnancy and diet interact to influence the composition of the maternal gut microbiota. Female C57BL/6 mice were fed either a control or a high fat diet for 8 weeks prior to mating. After confirmation of pregnancy, maternal weight gain and food intake were recorded. Fecal pellets were collected at 2 timepoints prior to mating (at the beginning of the experiment, and after 6 weeks of the specified diet) and at 4 timepoints during pregnancy (gestation day 0.5, 5.5, 10.5, and 15.5). The microbial composition and predicted metabolic functionality of the non-pregnant and pregnant gut was determined via sequencing of the variable 3 region of the 16S rRNA gene. Upon conception, differences in gut microbial communities were observed in both control and high fat-fed mice, including an increase in mucin-degrading bacteria. Control versus high fat-fed pregnant mice possessed the most profound changes to their maternal gut microbiota as indicated by statistically significant taxonomic differences. High fat-fed pregnant mice, when compared to control-fed animals, were found to be significantly enriched in microbes involved in metabolic pathways favoring fatty acid, ketone, vitamin, and bile synthesis. We show that pregnancy-induced changes in the female gut microbiota occur immediately at the onset of pregnancy, are vulnerable to modulation by diet, but are not dependent upon increases in maternal weight gain during pregnancy. High fat diet intake before and during pregnancy results in distinctive shifts in the pregnant gut microbiota in a gestational-age dependent manner and these shifts predict significant differences in the abundance of genes that favor lipid metabolism, glycolysis and gluconeogenic metabolic pathways over the course of pregnancy.
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“In addition, beyond FMO3 expression, microbial community alterations are known to occur during pregnancy145.”
From Gut microbiota and cardiovascular disease. (2020) · cites this paper as [145]
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Metadata
- Authors
- Gohir, W., Whelan, F. J., Surette, M. G., Moore, C., Schertzer, J. D., & Sloboda, D. M.
- Affiliations
- McMaster University (CA)
- Corresponding author
- Sloboda, Deborah M
- Venue
- Gut Microbes
- Publisher
- Informa UK Limited
- Published
- 2015-08-31
- Volume
- 6
- Issue
- 5
- Pages
- 310-320
- ISSN
- 1949-0976 (ISSN-L)
- 1949-0984
- PMC
- PMC4826136
- OpenAlex
- W2168621640
- Cited by
- 203
- References
- 51
- Citations by year
- 2026: 13 · 2025: 15 · 2024: 18 · 2023: 26 · 2022: 21 · 2021: 23 · 2020: 21 · 2019: 32 · 2018: 16 · 2017: 13 · 2016: 4 · 2015: 1
- FWCI
- 4.92
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Gut Microbes (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Repository@Nottingham (University of Nottingham) (submittedVersion, cc-by)
- Repository@Nottingham (University of Nottingham) (submittedVersion)
- Research Explorer (The University of Manchester) (submittedVersion, other-oa)
- Funders
- University of Manitoba
- Canadian Institutes of Health Research
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Health Sciences › Nursing › Nutrition and Dietetics › Infant Nutrition and Health
- Health Sciences › Dentistry › Periodontics › Oral microbiology and periodontitis research
- Keywords
- maternal gut microbiota, pregnancy, high-fat diet, gut microbiota composition, mucin-degrading bacteria, gestational weight gain, gestational age, lipid metabolism, glycolysis, gluconeogenesis, ketone body metabolism, vitamin metabolism, C57BL/6 mice
- MeSH terms
- Gastrointestinal Microbiome, Animals, Diet, DNA, Ribosomal, Feces, Female, Mice, Inbred C57BL, Pregnancy, RNA, Ribosomal, 16S, Sequence Analysis, DNA
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