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Cited by 425

The DOI matches its Crossref record; 58 of the 58 references it cites were found in a registry or an index.

The synaptic vesicle cycle: a single vesicle budding step involving clathrin and dynamin.

Open access
Takei, K, Mundigl, O, Daniell, L, De Camilli, P·1996·The Journal of cell biology
DOI 10.1083/jcb.133.6.1237
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Abstract

Strong evidence implicates clathrin-coated vesicles and endosome-like vacuoles in the reformation of synaptic vesicles after exocytosis, and it is generally assumed that these vacuoles represent a traffic station downstream from clathrin-coated vesicles. To gain insight into the mechanisms of synaptic vesicle budding from endosome-like intermediates, lysed nerve terminals and nerve terminal membrane subfractions were examined by EM after incubations with GTP gamma S. Numerous clathrin-coated budding intermediates that were positive for AP2 and AP180 immunoreactivity and often collared by a dynamin ring were seen. These were present not only on the plasma membrane (Takei, K., P.S. McPherson, S.L.Schmid, and P. De Camilli. 1995. Nature (Lond.). 374:186-190), but also on internal vacuoles. The lumen of these vacuoles retained extracellular tracers and was therefore functionally segregated from the extracellular medium, although narrow connections between their membranes and the plasmalemma were sometimes visible by serial sectioning. Similar observations were made in intact cultured hippocampal neurons exposed to high K+ stimulation. Coated vesicle buds were generally in the same size range of synaptic vesicles and positive for the synaptic vesicle protein synaptotagmin. Based on these results, we suggest that endosome-like intermediates of nerve terminals originate by bulk uptake of the plasma membrane and that clathrin- and dynamin-mediated budding takes place in parallel from the plasmalemma and from these internal membranes. We propose a synaptic vesicle recycling model that involves a single vesicle budding step mediated by clathrin and dynamin.

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Metadata

Authors
Takei, K., Mundigl, O., Daniell, L., & De Camilli, P.
Venue
The Journal of cell biology
Publisher
Rockefeller University Press
Published
1996-06-15
Volume
133
Issue
6
Pages
1237-1250
ISSN
  • 0021-9525 (ISSN-L)
  • 1540-8140
DOI
10.1083/jcb.133.6.1237
OpenAlex
W2038045605
Cited by
425
References
58
FWCI
11.61
Type
journal article
Language
en
Open access
green

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