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Numb Is an Endocytic Protein

Santolini, Elisa, Puri, Claudia, Salcini, Anna Elisabetta, Gagliani, Maria Cristina, Pelicci, Pier Giuseppe, Tacchetti, Carlo, Di Fiore, Pier Paolo·2000·The Journal of Cell Biology
DOI 10.1083/jcb.151.6.1345PMC PMC2190585
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Abstract

Numb is a protein that in Drosophila determines cell fate as a result of its asymmetric partitioning at mitosis. The function of Numb has been linked to its ability to bind and to biologically antagonize Notch, a membrane receptor that also specifies cell fate. The biochemical mechanisms underlying the action of Numb, however, are still largely unknown. The wide pattern of expression of Numb suggests a general function in cellular homeostasis that could be additional to, or part of, its action in fate determination. Such a function could be endocytosis, as suggested by the interaction of Numb with Eps15, a component of the endocytic machinery. Here, we demonstrate that Numb is an endocytic protein. We found that Numb localizes to endocytic organelles and is cotrafficked with internalizing receptors. Moreover, it associates with the appendage domain of α adaptin, a subunit of AP2, a major component of clathrin-coated pits. Finally, fragments of Numb act as dominant negatives on both constitutive and ligand-regulated receptor-mediated internalization, suggesting a general role for Numb in the endocytic process.

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Research works printed but not linked: 33

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Metadata

Authors
Santolini, E., Puri, C., Salcini, A. E., Gagliani, M. C., Pelicci, P. G., Tacchetti, C., & Di Fiore, P. P.
Venue
The Journal of Cell Biology
Publisher
Rockefeller University Press
Published
2000-12-11
Volume
151
Issue
6
Pages
1345-1352
ISSN
  • 0021-9525
  • 1540-8140
DOI
10.1083/jcb.151.6.1345
PMC
PMC2190585
Cited by
331
References
0
Language
en

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