The DOI matches its Crossref record; 41 of the 41 references it cites were found in a registry or an index.
Essential requirement for two-pore channel 1 in NAADP-mediated calcium signaling
Open accessAbstract
Nicotinic acid adenine dinucleotide phosphate (NAADP) is a widespread and potent calcium-mobilizing messenger that is highly unusual in activating calcium channels located on acidic stores. However, the molecular identity of the target protein is unclear. In this study, we show that the previously uncharacterized human two-pore channels (TPC1 and TPC2) are endolysosomal proteins, that NAADP-mediated calcium signals are enhanced by overexpression of TPC1 and attenuated after knockdown of TPC1, and that mutation of a single highly conserved residue within a putative pore region abrogated calcium release by NAADP. Thus, TPC1 is critical for NAADP action and is likely the long sought after target channel for NAADP.
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Gapped BLAST and PSI-BLAST: a new generation of protein database search programs
Altschul, S. (1997) · Nucleic Acids Research · doi:10.1093/nar/25.17.3389 · Cited by 73,302
The versatility and universality of calcium signalling
Berridge, M. J., Lipp, P., & Bootman, M. D. (2000) · Nature Reviews Molecular Cell Biology · doi:10.1038/35036035 · Cited by 5,701
Gene Splicing by Overlap Extension: Tailor-Made Genes Using the Polymerase Chain Reaction
Horton, R. M., Cai, Z., Ho, S. N., & Pease, L. R. (2013) · BioTechniques · doi:10.2144/000114017 · Cited by 1,289
Inositol Trisphosphate Receptor Ca2+Release Channels
Foskett, J. K., White, C., Cheung, K.-H., & Mak, D.-O. D. (2007) · Physiological Reviews · doi:10.1152/physrev.00035.2006 · Cited by 1,188
Ryanodine Receptor Calcium Release Channels
Fill, M., & Copello, J. A. (2002) · Physiological Reviews · doi:10.1152/physrev.00013.2002 · Cited by 1,099
NAADP mobilizes calcium from acidic organelles through two-pore channels
Calcraft, P. J., Ruas, M., Pan, Z., Cheng, X., Arredouani, A., Hao, X., Tang, J., Rietdorf, K., Teboul, L., Chuang, K.-T., et al. (2009) · Nature · doi:10.1038/nature08030 · Cited by 760
The type IV mucolipidosis-associated protein TRPML1 is an endolysosomal iron release channel
Dong, X.-P., Cheng, X., Mills, E., Delling, M., Wang, F., Kurz, T., & Xu, H. (2008) · Nature · doi:10.1038/nature07311 · Cited by 581
The vacuolar Ca2+-activated channel TPC1 regulates germination and stomatal movement
Peiter, E., Maathuis, F. J. M., Mills, L. N., Knight, H., Pelloux, J., Hetherington, A. M., & Sanders, D. (2005) · Nature · doi:10.1038/nature03381 · Cited by 538
Lee, H. C., & Aarhus, R. (1995) · Journal of Biological Chemistry · doi:10.1074/jbc.270.5.2152 · Cited by 475
NAADP Mobilizes Ca2+ from Reserve Granules, Lysosome-Related Organelles, in Sea Urchin Eggs
Churchill, G. C., Okada, Y., Thomas, J. M., Genazzani, A. A., Patel, S., & Galione, A. (2002) · Cell · doi:10.1016/s0092-8674(02)01082-6 · Cited by 457
Self-citation
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Patel, S., & Docampo, R. (2010) · Trends in Cell Biology · doi:10.1016/j.tcb.2010.02.003 · Cited by 237
Self-citationRegulation of lysosomal ion homeostasis by channels and transporters
Xiong, J., & Zhu, M. X. (2016) · Science China Life Sciences · doi:10.1007/s11427-016-5090-x · Cited by 141
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Metadata
- Authors
- Brailoiu, E., Churamani, D., Cai, X., Schrlau, M. G., Brailoiu, G. C., Gao, X., Hooper, R., Boulware, M. J., Dun, N. J., Marchant, J. S., & Patel, S.
- Venue
- Journal of Cell Biology
- Publisher
- Rockefeller University Press
- Published
- 2009-07-20
- Volume
- 186
- Issue
- 2
- Pages
- 201-209
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2112045725
- Cited by
- 401
- References
- 41
- FWCI
- 18.90
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Brailoiu, E., Churamani, D., Cai, X., Schrlau, M. G., Brailoiu, G. C., Gao, X., Hooper, R., Boulware, M. J., Dun, N. J., Marchant, J. S., & Patel, S. (2009). Essential requirement for two-pore channel 1 in NAADP-mediated calcium signaling. Journal of Cell Biology. https://doi.org/10.1083/jcb.200904073
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