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Cited by 16

The DOI matches its Crossref record; 54 of the 54 references it cites were found in a registry or an index.

Counterselection against Dμ Is Mediated through Immunoglobulin (Ig)α-Igβ

Gong, Shiaoching, Sanchez, Mercedes, Nussenzweig, Michel C.·1996·The Journal of Experimental Medicine
DOI 10.1084/jem.184.6.2079PMC PMC2196397
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Abstract

The pre-B cell receptor is a key checkpoint regulator in developing B cells. Early events that are controlled by the pre-B cell receptor include positive selection for cells express membrane immunoglobulin heavy chains and negative selection against cells expressing truncated immunoglobulins that lack a complete variable region (Dμ). Positive selection is known to be mediated by membrane immunoglobulin heavy chains through Igα-Igβ, whereas the mechanism for counterselection against Dμ has not been determined. We have examined the role of the Igα-Igβ signal transducers in counterselection against Dμ using mice that lack Igβ. We found that Dμ expression is not selected against in developing B cells in Igβ mutant mice. Thus, the molecular mechanism for counterselection against Dμ in pre-B cells resembles positive selection in that it requires interaction between mDμ and Igα-Igβ.

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Figures

Figure 1 Flow cytometric analysis of spleen (Spl) and bone marrow cells (BM) from Igβ−/−, transgenic, and wild-type mice. Single-cell suspensions from lymphoid organs of 6-wkold mice were prepared and analyzed on FACScan®. Bone marrow cells were stained with PE–anti-B220 and FITC–anti-CD43. Spleen cells were stained with FITC–anti-IgM and PE– anti-B220. The lymphocyte population was gated according to standard forward- and side-scatter values. The numbers in each quadrant represent the percentages of gated lymphocytes. WT, wild type; RAG −/−, RAG-1 mutant; Igβ −/−, Igβ mutant; Igβ −/− μ.TG, Igβ mutant, mIgμ, transgenic.
Figure 1 Flow cytometric analysis of spleen (Spl) and bone marrow cells (BM) from Igβ−/−, transgenic, and wild-type mice. Single-cell suspensions from lymphoid organs of 6-wkold mice were prepared and analyzed on FACScan®. Bone marrow cells were stained with PE–anti-B220 and FITC–anti-CD43. Spleen cells were stained with FITC–anti-IgM and PE– anti-B220. The lymphocyte population was gated according to standard forward- and side-scatter values. The numbers in each quadrant represent the percentages of gated lymphocytes. WT, wild type; RAG −/−, RAG-1 mutant; Igβ −/−, Igβ mutant; Igβ −/− μ.TG, Igβ mutant, mIgμ, transgenic.CC BY-NC-SA · PubMed Central
Figure 2 Ig gene rearrangements in Igβ−/−, transgenic, and wild-type mice. Bone marrow DNA from 6-wk-old mice was amplified with VH558L, VH7183 (not shown), or DH and JH2 primers. Control primers were from the J-CH1 intervening sequence (IVS) (22).
Figure 2 Ig gene rearrangements in Igβ−/−, transgenic, and wild-type mice. Bone marrow DNA from 6-wk-old mice was amplified with VH558L, VH7183 (not shown), or DH and JH2 primers. Control primers were from the J-CH1 intervening sequence (IVS) (22).CC BY-NC-SA · PubMed Central
Figure 3 Nucleotide sequences of DH–JH joints from Igβ−/− and wild-type sorted bone marrow B cells. DH segment, N or P nucleotides, and JH4 segment sequences are shown. The number of DJH4 joints in DH RF 1, 2, or 3 are noted. Stop codons in DH segments are underlined.
Figure 3 Nucleotide sequences of DH–JH joints from Igβ−/− and wild-type sorted bone marrow B cells. DH segment, N or P nucleotides, and JH4 segment sequences are shown. The number of DJH4 joints in DH RF 1, 2, or 3 are noted. Stop codons in DH segments are underlined.CC BY-NC-SA · PubMed Central
Figure 4 Nucleotide sequences of V(D)J (VH558L to DJH4) joints from Igβ−/− and wild-type bone marrow B cells. DH segment, N or P nucleotides, and JH4 segment sequences are shown. The number of DJH4 joints in DH RF 1, 2, or 3 are noted. Stop codons in DH segments are underlined, and the DH segments used (S) are indicated.
Figure 4 Nucleotide sequences of V(D)J (VH558L to DJH4) joints from Igβ−/− and wild-type bone marrow B cells. DH segment, N or P nucleotides, and JH4 segment sequences are shown. The number of DJH4 joints in DH RF 1, 2, or 3 are noted. Stop codons in DH segments are underlined, and the DH segments used (S) are indicated.CC BY-NC-SA · PubMed Central

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Metadata

Authors
Gong, S., Sanchez, M., & Nussenzweig, M. C.
Affiliations
From the Laboratory of Molecular Immunology, The Howard Hughes Medical Institute, The Rockefeller University, New York 10021
Venue
The Journal of Experimental Medicine
Publisher
Rockefeller University Press
Published
1996-12-01
Volume
184
Issue
6
Pages
2079-2084
ISSN
  • 0022-1007
  • 1540-9538
DOI
10.1084/jem.184.6.2079
PMC
PMC2196397
Cited by
16
References
54
Language
en

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