The DOI matches its Crossref record; 52 of the 52 references it cites were found in a registry or an index.
STAT5 is a potent negative regulator of TFH cell differentiation
Open accessAbstract
Follicular helper T cells (TFH cells) constitute the CD4+ T cell subset that is specialized to provide help to germinal center (GC) B cells and, consequently, mediate the development of long-lived humoral immunity. TFH cell differentiation is driven by the transcription factor Bcl6, and recent studies have identified cytokine and cell–cell signals that drive Bcl6 expression. However, although TFH dysregulation is associated with several major autoimmune diseases, the mechanisms underlying the negative regulation of TFH cell differentiation are poorly understood. In this study, we show that STAT5 inhibits TFH cell differentiation and function. Constitutive STAT5 signaling in activated CD4+ T cells selectively blocked TFH cell differentiation and GCs, and IL-2 signaling was a primary inducer of this pathway. Conversely, STAT5-deficient CD4+ T cells (mature STAT5fl/fl CD4+ T cells transduced with a Cre-expressing vector) rapidly up-regulated Bcl6 expression and preferentially differentiated into TFH cells during T cell priming in vivo. STAT5 signaling failed to inhibit TFH cell differentiation in the absence of the transcription factor Blimp-1, a direct repressor of Bcl6 expression and TFH cell differentiation. These results demonstrate that IL-2, STAT5, and Blimp-1 collaborate to negatively regulate TFH cell differentiation.
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Cites
Differentiation of Effector CD4 T Cell Populations
Zhu, J., Yamane, H., & Paul, W. E. (2010) · Annual Review of Immunology · doi:10.1146/annurev-immunol-030409-101212 · Cited by 3,452
Follicular Helper CD4 T Cells (T FH )
Crotty, S. (2011) · Annual Review of Immunology · doi:10.1146/annurev-immunol-031210-101400 · Cited by 2,738
Self-citationJohnston, R. J., Poholek, A. C., DiToro, D., Yusuf, I., Eto, D., Barnett, B., Dent, A. L., Craft, J., & Crotty, S. (2009) · Science · doi:10.1126/science.1175870 · Cited by 1,626
Self-citationBcl6 mediates the development of T follicular helper cells
Nurieva, R. I., Chung, Y., Martinez, G. J., Yang, X. O., Tanaka, S., Matskevitch, T. D., Wang, Y.-H., & Dong, C. (2009) · Science · doi:10.1126/science.1176676 · Cited by 1,506
The Transcriptional Repressor Bcl-6 Directs T Follicular Helper Cell Lineage Commitment
Yu, D., Rao, S., Tsai, L. M., Lee, S. K., He, Y., Sutcliffe, E. L., Srivastava, M., Linterman, M., Zheng, L., Simpson, N., et al. (2009) · Immunity · doi:10.1016/j.immuni.2009.07.002 · Cited by 1,228
Nurieva, R. I., Chung, Y., Hwang, D., Yang, X. O., Kang, H. S., Ma, L., Wang, Y.-H., Watowich, S. S., Jetten, A. M., Tian, Q., et al. (2008) · Immunity · doi:10.1016/j.immuni.2008.05.009 · Cited by 1,205
Interleukin-2 receptor α chain regulates the size and content of the peripheral lymphoid compartment
Willerford, D. M., Chen, J., Ferry, J. A., Davidson, L., Ma, A., & Alt, F. W. (1995) · Immunity · doi:10.1016/1074-7613(95)90180-9 · Cited by 1,080
New insights into the regulation of T cells by γc family cytokines
Rochman, Y., Spolski, R., & Leonard, W. J. (2009) · Nature Reviews Immunology · doi:10.1038/nri2580 · Cited by 1,053
Choi, Y. S., Kageyama, R., Eto, D., Escobar, T. C., Johnston, R. J., Monticelli, L., Lao, C., & Crotty, S. (2011) · Immunity · doi:10.1016/j.immuni.2011.03.023 · Cited by 958
Self-citationShapiro-Shelef, M., Lin, K.-I., McHeyzer-Williams, L. J., Liao, J., McHeyzer-Williams, M. G., & Calame, K. (2003) · Immunity · doi:10.1016/s1074-7613(03)00267-x · Cited by 839
Cited by
T follicular helper cell differentiation, function, and roles in disease
Crotty, S. (2014) · Immunity · doi:10.1016/j.immuni.2014.10.004 · Cited by 1,847
Self-citationT follicular helper cell biology: a decade of discovery and diseases
Crotty, S. (2019) · Immunity · doi:10.1016/j.immuni.2019.04.011 · Cited by 1,591
Self-citationMechanisms and consequences of Jak–STAT signaling in the immune system
Villarino, A. V., Kanno, Y., & O'Shea, J. J. (2017) · Nature Immunology · doi:10.1038/ni.3691 · Cited by 1,221
Vinuesa, C. G., Linterman, M. A., Yu, D., & MacLennan, I. C. M. (2016) · Annual Review of Immunology · doi:10.1146/annurev-immunol-041015-055605 · Cited by 1,141
Interleukin-2 at the Crossroads of Effector Responses, Tolerance, and Immunotherapy
Liao, W., Lin, J.-X., & Leonard, W. J. (2013) · Immunity · doi:10.1016/j.immuni.2013.01.004 · Cited by 1,075
Metadata
- Authors
- Johnston, R. J., Choi, Y. S., Diamond, J. A., Yang, J. A., & Crotty, S.
- Venue
- Journal of Experimental Medicine
- Publisher
- Rockefeller University Press
- Published
- 2012-01-23
- Volume
- 209
- Issue
- 2
- Pages
- 243-250
- ISSN
- 0022-1007 (ISSN-L)
- 1540-9538
- OpenAlex
- W2096475468
- Cited by
- 484
- References
- 52
- FWCI
- 17.13
- Type
- journal article
- Language
- en
- Open access
- green
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- Johnston, R. J., Choi, Y. S., Diamond, J. A., Yang, J. A., & Crotty, S. (2012). STAT5 is a potent negative regulator of TFH cell differentiation. Journal of Experimental Medicine. https://doi.org/10.1084/jem.20111174
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