Interleukin-10 Inhibits Spontaneous Sleep in Rabbits
Abstract
Proinflammatory cytokines, including interleukin-1beta(IL-1beta) and tumor necrosis factor-alpha (TNF-alpha) are involved in sleep regulation. IL-10 is an anti-inflammatory cytokine that inhibits proinflammatory cytokine production. We hypothesized that IL-10 could attenuate sleep. Thirty-one male rabbits were used. Three doses of IL-10 (5 ng, 50 ng, and 250 ng) were injected intracerebroventricularly during the rest (light) period. One dose of IL-10 (250 ng) was injected during the active (dark) cycle. Appropriate time-matched control injections of saline were given to the same rabbits on different days. The two highest doses of IL-10 significantly inhibited spontaneous nonrapid eye movement sleep if IL-10 was given during the light cycle. The highest dose of IL-10 (250 ng) also significantly decreased rapid eye movement sleep. IL-10 administered at dark onset had no effect on sleep. The sleep inhibitory properties of IL-10 provide additional evidence for the hypothesis that a brain cytokine network is involved in regulation of physiologic sleep.
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The anti-inflammatory cytokines IL-4, IL-10, and IL-13 attenuate NREM sleep amount in rabbits.
“The anti-inflammatory cytokines IL-4, IL-10, and IL-13 have been reported to attenuate NREM sleep amount in rabbits (308, 314, 315)”
From The Sleep-Immune Crosstalk in Health and Disease (2019) · cites this paper as [315]
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- Authors
- Kushikata, T., Fang, J., & Krueger, J. M.
- Affiliations
- Washington State University (US)
- ORCID
- Kushikata, Tetsuya
- Venue
- Journal of Interferon & Cytokine Research
- Publisher
- SAGE Publications
- Published
- 1999-09
- Volume
- 19
- Issue
- 9
- Pages
- 1025-1030
- ISSN
- 1079-9907 (ISSN-L)
- 1557-7465
- OpenAlex
- W3024296926
- Cited by
- 69
- References
- 29
- Citations by year
- 2026: 2 · 2025: 2 · 2024: 4 · 2023: 1 · 2022: 3 · 2021: 3 · 2020: 2 · 2019: 3 · 2017: 4 · 2016: 4 · 2013: 3 · 2012: 1
- FWCI
- 2.58
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Interferon & Cytokine Research (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health
- National Institute of Neurological Disorders and Stroke (NS-31453, NS-25378, R01NS031453, R01NS025378)
- Eunice Kennedy Shriver National Institute of Child Health and Human Development (HD-36520, R01HD036520)
- Topics
- Life Sciences › Neuroscience › Cognitive Neuroscience › Sleep and Wakefulness Research
- Life Sciences › Neuroscience › Endocrine and Autonomic Systems › Circadian rhythm and melatonin
- Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
- Keywords
- IL-10, sleep regulation, NREM sleep, REM sleep, cytokines, anti-inflammatory cytokines, inflammatory cytokines, intracerebroventricular administration, circadian rhythm, rabbits
- MeSH terms
- Analysis of Variance, Animals, Body Temperature Regulation, Drug Evaluation, Preclinical, Electroencephalography, Humans, Male, Rabbits, Recombinant Proteins, Sleep, Interleukin-10
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