Inhibition of human immunodeficiency virus type 1 by lactic acid bacteria from human breastmilk
Open accessAbstract
BACKGROUND: Human breastmilk provides a rich source of commensal lactic acid bacteria (LAB) to the infant during breastfeeding and stimulates abundant growth and colonization of these bacteria at mucosal surfaces in the infant gastrointestinal tract. While conferring critical nutritional and immunologic support to the developing newborn, breastmilk also serves as a vehicle for human immunodeficiency virus type 1 (HIV-1) transmission from mother to child during breastfeeding. Whether breastmilk LAB confer protection against mucosal exposure to HIV-1 in breastfeeding infants is unknown. STUDY DESIGN: In the present study, we sought to evaluate LAB isolated from the breastmilk of healthy women for the ability to inhibit HIV-1 infection in vitro. A total of 38 strains of breastmilk bacteria were evaluated in this study. Both heat-killed bacteria and cell-free conditioned supernatants from bacterial cultures were tested for the ability to inhibit infection with HIV-1 using viral isolates with tropism for CCR5 (R5), CXCR4 (X4), or R5/X4 dual-tropism. RESULTS: Significant inhibition of R5-tropic HIV-1 was demonstrated using heat-killed bacteria, most notably among breastmilk strains of Lactobacillus and Pediococcus. Selected strains of breastmilk LAB also demonstrated significant inhibition of HIV-1 infection against virus with tropism for X4 and R5/X4. CONCLUSION: These results demonstrate for the first time that commensal LAB from human breastmilk inhibit HIV-1 infection in vitro and suggest a possible role for these bacteria in mucosal protection against HIV-1 in the breastfeeding infant.
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Other metabolites and bacterial cell fragments may interfere with virus binding, and Levilactobacillus brevis cell wall fragments have been reported to inhibit herpes simplex replication.
“Also other metabolites and bacterial cell fragments may interfere with virus binding (Martín et al., 2010) and Levilactobacillus brevis cell wall fragments have been reported to inhibit herpes simplex replication (Mastromarino et al., 2011).”
From Viral Infections, the Microbiome, and Probiotics (2021) · cites this paper as Martín et al., 2010
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Cites
Bacteriocins: Developing innate immunity for food
Cotter, P. D., Hill, C., & Ross, R. P. (2005) · Nature Reviews Microbiology · doi:10.1038/nrmicro1273 · Cited by 2,380 · see citing works
Human milk is a source of lactic acid bacteria for the infant gut
Martín, R., Langa, S., Reviriego, C., Jimínez, E., Marín, M. L., Xaus, J., Fernández, L., & Rodríguez, J. M. (2003) · The Journal of Pediatrics · doi:10.1016/j.jpeds.2003.09.028 · Cited by 792 · see citing works
Self-citationThe role of sexually transmitted diseases in HIV transmission
Galvin, S., & Cohen, M. S. (2004) · Nature Reviews Microbiology · doi:10.1038/nrmicro794 · Cited by 782 · see citing works
The Continuing Story of Class IIa Bacteriocins
Drider, D., Fimland, G., Héchard, Y., McMullen, L. M., & Prévost, H. (2006) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.00016-05 · Cited by 750 · see citing works
Bacteriocins from lactic acid bacteria: Production, purification, and food applications
De Vuyst, L., & Leroy, F. (2007) · Microbial Physiology · doi:10.1159/000104752 · Cited by 708 · see citing works
Inhibition of Staphylococcus aureus by the commensal bacteria of human milk
Heikkilä, M., & Saris, P. E. J. (2003) · Journal of Applied Microbiology · doi:10.1046/j.1365-2672.2003.02002.x · Cited by 501 · see citing works
The immune system of human milk
Goldman, A. S. (1993) · The Pediatric Infectious Disease Journal · doi:10.1097/00006454-199308000-00008 · Cited by 475 · see citing works
Eijsink, V. G. H., Axelsson, L. T., Diep, D. B., Håvarstein, L. S., Holo, H., & Nes, I. F. (2002) · Antonie van Leeuwenhoek · doi:10.1023/a:1020582211262 · Cited by 352 · see citing works
Probiotic Potential of 3 Lactobacilli Strains Isolated From Breast Milk
Martı́n, R., Olivares, M. M., Marín, M. L., Fernández, L., Xaus, J., & Rodríguez, J. M. (2005) · Journal of Human Lactation · doi:10.1177/0890334404272393 · Cited by 279 · see citing works
Self-citationBreast Milk: A Source of Bifidobacteria for Infant Gut Development and Maturation?
Gueimonde, M., Laitinen, K., Salminen, S., & Isolauri, E. (2007) · Neonatology · doi:10.1159/000100088 · Cited by 273 · see citing works
Cultivation-independent assessment of the bacterial diversity of breast milk among healthy women
Martı́n, R., Heilig, H. G. H. J., Zoetendal, E. G., Jiménez, E., Fernández, L., Smidt, H., & Rodríguez, J. M. (2006) · Research in Microbiology · doi:10.1016/j.resmic.2006.11.004 · Cited by 261 · see citing works
Self-citationMartı́n, R., Heilig, G. H. J., Zoetendal, E. G., Smidt, H., & Rodríguez, J. M. (2007) · Journal of Applied Microbiology · doi:10.1111/j.1365-2672.2007.03497.x · Cited by 239 · see citing works
Self-citationFimland, G., Johnsen, L., Dalhus, B., & Nissen‐Meyer, J. (2005) · Journal of Peptide Science · doi:10.1002/psc.699 · Cited by 227 · see citing works
Antimicrobial potential of four Lactobacillus strains isolated from breast milk
Olivares, M. M., Díaz-Ropero, M. P., Martı́n, R., Rodríguez, J. M., & Xaus, J. (2006) · Journal of Applied Microbiology · doi:10.1111/j.1365-2672.2006.02981.x · Cited by 226 · see citing works
Self-citationProbiotics to enhance anti-infective defences in the gastrointestinal tract
Gill, H. S. (2003) · Best Practice & Research Clinical Gastroenterology · doi:10.1016/s1521-6918(03)00074-x · Cited by 224 · see citing works
Jiménez, E., Fernández, L., Maldonado‐Barragán, A., Martı́n, R., Olivares, M. M., Xaus, J., & Rodríguez, J. M. (2008) · Applied and Environmental Microbiology · doi:10.1128/aem.02599-07 · Cited by 219 · see citing works
Self-citationStaphylococcus epidermidis: A differential trait of the fecal microbiota of breast-fed infants
Jiménez, E., Delgado, S., Maldonado‐Barragán, A., Arroyo, R., Albújar, M., García, N., Jariod, M., Fernández, L., Gómez, A., & Rodríguez, J. M. (2008) · BMC Microbiology · doi:10.1186/1471-2180-8-143 · Cited by 170 · see citing works
Self-citationMartı́n, R., Jiménez, E., Olivares, M. M., Marín, M. L., Fernández, L., Xaus, J., & Rodríguez, J. M. (2006) · International Journal of Food Microbiology · doi:10.1016/j.ijfoodmicro.2006.06.011 · Cited by 167 · see citing works
Self-citationEnterocin CRL35 inhibits late stages of HSV-1 and HSV-2 replication in vitro
Wachsman, M. B., Castilla, V., Holgado, A. P. D. R., Torres, R. A. D., Sesma, F., & Coto, C. E. (2003) · Antiviral Research · doi:10.1016/s0166-3542(02)00099-2 · Cited by 161 · see citing works
Perán, L., Camuesco, D., Comalada, M., Nieto, A., Concha, Á., Díaz-Ropero, M. P., Olivares, M. M., Xaus, J., Zarzuelo, A., & Gálvez, J. (2005) · Europe PMC (PubMed Central) · doi:10.3748/wjg.v11.i33.5185 · Cited by 136 · see citing works
Beneficial effects of probiotic bacteria isolated from breast milk
Lara‐Villoslada, F., Olivares, M. M., Sierra, S., Rodríguez, J. M., Bozá, J., & Xaus, J. (2007) · British Journal Of Nutrition · doi:10.1017/s0007114507832910 · Cited by 120 · see citing works
Self-citationJiménez, E., Delgado, S., Fernández, L., García, N., Albújar, M., Gómez, A., & Rodríguez, J. M. (2008) · Research in Microbiology · doi:10.1016/j.resmic.2008.09.001 · Cited by 103 · see citing works
Self-citationOlivares, M., Paz Díaz-Ropero, M., Gómez, N., Sierra, S., Lara-Villoslada, F., Martín, R., Miguel Rodríguez, J., & Xaus, J. (2006) · Journal of Dairy Research · doi:10.1017/s0022029906002068 · Cited by 68 · see citing works
Kourtis, A. P., Jamieson, D. J., de Vincenzi, I., et al. (2007) · American Journal of Obstetrics and Gynecology · doi:10.1016/j.ajog.2007.03.003 · Cited by 63 · see citing works
Mastitis and Transmission of Human Immunodeficiency Virus through Breast Milk
Semba, R. D. (2000) · Annals of the New York Academy of Sciences · doi:10.1111/j.1749-6632.2000.tb05484.x · Cited by 62 · see citing works
Genital Tract Infections and Perinatal Transmission of HIV
Taha, T. E., & Gray, R. H. (2000) · Annals of the New York Academy of Sciences · doi:10.1111/j.1749-6632.2000.tb05477.x · Cited by 55 · see citing works
Lyimo, M. A., Howell, A. L., Balandya, E., et al. (2009) · JAIDS Journal of Acquired Immune Deficiency Syndromes · doi:10.1097/qai.0b013e3181a3908d · Cited by 29 · see citing works
Flagged first, then by citations
Research works printed but not linked: 2
- [16]Carlson SJ. Pavlova SI. Spear GT, et al. IADR/AADR/CADR 82nd General Session. Honolulu, HI: 2004. Lactobacillus lectin as a natural HIV trap [abstract 2280] [Google Scholar]
- [17]Chang R. Pavlova S. Caffrey M, et al. The Mouth and AIDS: The Global Challenge 6th World Workshop on Oral Health and Disease. Beijing: 2009. Blocking milk-borne HIV transmission by virus-capturing lactobacilli. [Google Scholar]
Cited by
Sorted by citations
Human milk: a source of more life than we imagine
Jeurink, P. V., van Bergenhenegouwen, J., Jiménez, E., Knippels, L. M. J., Fernández, L., Garssen, J., Knol, J., Rodríguez, J. M., & Martín, R. (2013) · Beneficial Microbes · doi:10.3920/bm2012.0040 · Cited by 354 · see citing works
Self-citationAntiviral Potential of Lactic Acid Bacteria and Their Bacteriocins
Al Kassaa, I., Hober, D., Hamze, M., Chihib, N. E., & Drider, D. (2014) · Probiotics and Antimicrobial Proteins · doi:10.1007/s12602-014-9162-6 · Cited by 217 · see citing works
Viral Infections, the Microbiome, and Probiotics
Harper, A., Vijayakumar, V., Ouwehand, A. C., ter Haar, J., Obis, D., Espadaler, J., Binda, S., Desiraju, S., & Day, R. (2021) · Frontiers in Cellular and Infection Microbiology · doi:10.3389/fcimb.2020.596166 · Cited by 177 · see citing works
Biliavska, L., Pankivska, Y., Povnitsa, O., & Zagorodnya, S. (2019) · Medicina · doi:10.3390/medicina55090519 · Cited by 81 · see citing works
Li, S., Li, N., Wang, C., Zhao, Y., Cao, J., Li, X., Zhang, Z., Li, Y., Yang, X., Wang, X., et al. (2022) · Frontiers in Nutrition · doi:10.3389/fnut.2022.798403 · Cited by 26 · see citing works
- Authors
- Martín, V., Maldonado, A., Fernández, L., Rodríguez, J. M., & Connor, R. I.
- Affiliations
- Universidad Complutense de Madrid (ES)
- Complutense University of Madrid
- Dartmouth College (US)
- Corresponding author
- Connor, Ruth I.
- Venue
- Breastfeeding Medicine
- Publisher
- SAGE Publications
- Published
- 2010-05-22
- Volume
- 5
- Issue
- 4
- Pages
- 153-158
- ISSN
- 1556-8253 (ISSN-L)
- 1556-8342
- PMC
- PMC2936252
- OpenAlex
- W2046243431
- Cited by
- 64
- References
- 27
- Citations by year
- 2026: 4 · 2025: 2 · 2024: 3 · 2023: 4 · 2022: 6 · 2021: 7 · 2020: 9 · 2019: 6 · 2018: 3 · 2017: 1 · 2016: 2 · 2015: 3 · 2014: 2 · 2013: 3 · 2012: 7
- FWCI
- 5.89
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Breastfeeding Medicine (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (5r21ai065235-03)
- National Institute of Allergy and Infectious Diseases (1R21AI065235)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Health Sciences › Nursing › Nutrition and Dietetics › Infant Nutrition and Health
- Life Sciences › Immunology and Microbiology › Virology › HIV Research and Treatment
- Keywords
- lactic acid bacteria, breast milk, HIV-1, antiviral activity, mother-to-child transmission, breastfeeding, Lactobacillus, Pediococcus, CCR5-tropic HIV-1, CXCR4-tropic HIV-1, dual-tropic HIV-1, mucosal protection, heat-killed bacteria, cell-free supernatant
- MeSH terms
- Cells, Cultured, Humans, Intestinal Mucosa, Lactobacillus, Milk, Human, HIV-1, HIV Infections, Infectious Disease Transmission, Vertical, Receptors, CCR5, Receptors, CXCR4, Virus Inactivation
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