Student or educator? Sign up with your school email and get 6 months of Pro free.
CoChat
Sign Up
Cited by 417

The DOI matches its Crossref record.

Apg7p/Cvt2p: A Novel Protein-activating Enzyme Essential for Autophagy

Open access
Tanida, Isei, Mizushima, Noboru, Kiyooka, Miho, Ohsumi, Mariko, Ueno, Takashi, Ohsumi, Yoshinori, Kominami, Eiki·1999·Molecular Biology of the Cell
DOI 10.1091/mbc.10.5.1367
Open this paper in CoChat — free account

Abstract

In the yeast Saccharomyces cerevisiae, the Apg12p–Apg5p conjugating system is essential for autophagy. Apg7p is required for the conjugation reaction, because Apg12p is unable to form a conjugate with Apg5p in the apg7/cvt2mutant. Apg7p shows a significant similarity to a ubiquitin-activating enzyme, Uba1p. In this article, we investigated the function of Apg7p as an Apg12p-activating enzyme. Hemagglutinin-tagged Apg12p was coimmunoprecipitated with c-myc–tagged Apg7p. A two-hybrid experiment confirmed the interaction. The coimmunoprecipitation was sensitive to a thiol-reducing reagent. Furthermore, a thioester conjugate of Apg7p was detected in a lysate of cells overexpressing both Apg7p and Apg12p. These results indicated that Apg12p interacts with Apg7p via a thioester bond. Mutational analyses of Apg7p suggested that Cys 507 of Apg7p is an active site cysteine and that both the ATP-binding domain and the cysteine residue are essential for the conjugation of Apg7p with Apg12p to form the Apg12p–Apg5p conjugate. Cells expressing mutant Apg7ps, Apg7p G333A, or Apg7p C507A showed defects in autophagy and cytoplasm-to-vacuole targeting of aminopeptidase I. These results indicated that Apg7p functions as a novel protein-activating enzyme necessary for Apg12p–Apg5p conjugation.

Overview

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1091/mbc.10.5.1367

Cites

Statistics

Verification

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Citations

Cites

No references could be resolved yet.

Research works printed but not linked: 78

  1. Baba M., 1995, Cell Struct. Funct., 20, 465, 10.1247/csf.20.465 · DOI · Paper page
  2. Baba M., 1994, J. Cell Biol., 124, 903, 10.1083/jcb.124.6.903 · DOI · Paper page
  3. Bartel B., 1990, EMBO J., 9, 3179, 10.1002/j.1460-2075.1990.tb07516.x · DOI · Paper page
  4. Boddy M.N., 1996, Oncogene, 13, 971
  5. Callis J., 1995, Genetics, 139, 921, 10.1093/genetics/139.2.921 · doi:10.1093/genetics/139.2.921
  6. Chow N., 1996, J. Biol. Chem., 271, 11339, 10.1074/jbc.271.19.11339 · DOI
  7. Ciechanover A., 1982, J. Biol. Chem., 257, 2537, 10.1016/S0021-9258(18)34957-3 · DOI · Paper page
  8. del Olmo M., 1997, Mol. Gen. Genet., 255, 209, 10.1007/s004380050491 · doi:10.1007/s004380050491
  9. del Pozo J.C., 1998, Science, 280, 1760, 10.1126/science.280.5370.1760 · DOI
  10. Dohmen R.J., 1995, J. Biol. Chem., 270, 18099, 10.1074/jbc.270.30.18099 · DOI
  11. Dolan L., 1998, Genes Dev., 12, 2091, 10.1101/gad.12.14.2091 · doi:10.1101/gad.12.14.2091
  12. Dunn W.A., 1994, Trends Cell Biol., 4, 139, 10.1016/0962-8924(94)90069-8 · DOI · Paper page
  13. Finley D., 1991, Annu. Rev. Cell Biol., 7, 25, 10.1146/annurev.cb.07.110191.000325 · DOI · Paper page
  14. Funakoshi T., 1997, Gene, 192, 207, 10.1016/S0378-1119(97)00031-0 · DOI
  15. Gong L., 1997, J. Biol. Chem., 272, 28198, 10.1074/jbc.272.45.28198 · DOI
  16. Haas A.L., 1997, FASEB J., 11, 1257, 10.1096/fasebj.11.14.9409544 · DOI · Paper page
  17. Haas A.L., 1982, J. Biol. Chem., 257, 2543, 10.1016/S0021-9258(18)34958-5 · DOI · Paper page
  18. Hammond E.M., 1998, FEBS Lett., 425, 391, 10.1016/S0014-5793(98)00266-X · DOI
  19. Handley P.M., 1991, Proc. Natl. Acad. Sci. USA, 88, 258, 10.1073/pnas.88.1.258 · DOI
  20. Harding T.M., 1996, J. Biol. Chem., 271, 17621, 10.1074/jbc.271.30.17621 · DOI · Paper page
  21. Hatfield P.M., 1990, J. Biol. Chem., 265, 15813, 10.1016/S0021-9258(18)55470-3 · DOI
  22. Hatfield P.M., 1997, Plant J., 11, 213, 10.1046/j.1365-313X.1997.11020213.x · doi:10.1046/j.1365-313x.1997.11020213.x
  23. Hatfield P.M., 1992, J. Biol. Chem., 267, 14799, 10.1016/S0021-9258(18)42110-2 · DOI
  24. Hershko A., 1996, Trends Biochem. Sci., 21, 445, 10.1016/S0968-0004(96)10054-2 · DOI
  25. Hershko A., 1992, Annu. Rev. Biochem., 61, 761, 10.1146/annurev.bi.61.070192.003553 · DOI · Paper page
  26. Hershko A., 1983, J. Biol. Chem., 258, 8206, 10.1016/S0021-9258(20)82050-X · DOI · Paper page
  27. Hochstrasser M., 1996, Cell, 84, 813, 10.1016/S0092-8674(00)81058-2 · DOI · Paper page
  28. Hochstrasser M., 1996, Annu. Rev. Genet., 30, 405, 10.1146/annurev.genet.30.1.405 · DOI · Paper page
  29. Hochstrasser M., 1998, Genes Dev., 12, 901, 10.1101/gad.12.7.901 · DOI
  30. Homma K., 1998, J. Biol. Chem., 273, 15779, 10.1074/jbc.273.25.15779 · DOI
  31. Huang P.-H., 1997, J. Cell Biol., 136, 803, 10.1083/jcb.136.4.803 · DOI · Paper page
  32. Imai N., 1992, Gene, 118, 279, 10.1016/0378-1119(92)90200-9 · DOI
  33. James P., 1996, Genetics, 144, 1425, 10.1093/genetics/144.4.1425 · DOI · Paper page
  34. Johnson E.S., 1997, J. Biol. Chem., 272, 26799, 10.1074/jbc.272.43.26799 · DOI · Paper page
  35. Johnson E.S., 1997, EMBO J., 16, 5509, 10.1093/emboj/16.18.5509 · DOI · Paper page
  36. Johnson P.R., 1997, Trends Cell Biol., 7, 408, 10.1016/S0962-8924(97)01132-X · DOI
  37. Kametaka S., 1996, Gene, 178, 139, 10.1016/0378-1119(96)00354-X · DOI
  38. Kametaka S., 1998, J. Biol. Chem., 273, 22284, 10.1074/jbc.273.35.22284 · DOI · Paper page
  39. Kamitani T., 1997, J. Biol. Chem., 272, 28552
  40. Klionsky D.J., 1992, J. Cell Biol., 119, 287, 10.1083/jcb.119.2.287 · DOI · Paper page
  41. Kumar S., 1993, Biochem. Biophys. Res. Commun., 195, 393, 10.1006/bbrc.1993.2056 · DOI
  42. Lammer D., 1998, Genes Dev., 7, 914, 10.1101/gad.12.7.914 · DOI · Paper page
  43. Lang T., 1998, EMBO J., 17, 3597, 10.1093/emboj/17.13.3597 · DOI · Paper page
  44. Lee G.W., 1998, J. Biol. Chem., 273, 6503, 10.1074/jbc.273.11.6503 · DOI
  45. Leyser D., 1993, Nature, 364, 161, 10.1038/364161a0 · DOI · Paper page
  46. Liakopoulos D., 1998, EMBO J., 17, 2208, 10.1093/emboj/17.8.2208 · DOI · Paper page
  47. Lorenz M.C., 1995, Gene, 158, 113, 10.1016/0378-1119(95)00144-U · DOI · Paper page
  48. Mahajan R., 1997, Cell, 88, 97, 10.1016/S0092-8674(00)81862-0 · DOI · Paper page
  49. Mahajan R., 1998, J. Cell Biol., 140, 259, 10.1083/jcb.140.2.259 · DOI · Paper page
  50. Matsuura A., 1997, Gene, 192, 245, 10.1016/S0378-1119(97)00084-X · DOI · Paper page
  51. Matunis M.J., 1998, J. Cell Biol., 140, 499, 10.1083/jcb.140.3.499 · DOI · Paper page
  52. Matunis M.J., 1996, J. Cell Biol., 135, 1457, 10.1083/jcb.135.6.1457 · DOI · Paper page
  53. McGrath J.P., 1991, EMBO J., 10, 227, 10.1002/j.1460-2075.1991.tb07940.x · DOI · Paper page
  54. Mizushima N., 1998, Nature, 395, 395, 10.1038/26506 · DOI · Paper page
  55. Mizushima N., 1998, J. Biol. Chem., 273, 33889, 10.1074/jbc.273.51.33889 · DOI · Paper page
  56. Muller S., 1998, EMBO J., 17, 61, 10.1093/emboj/17.1.61 · DOI · Paper page
  57. Noda T., 1998, J. Biol. Chem., 273, 3963, 10.1074/jbc.273.7.3963 · DOI · Paper page
  58. Okura T., 1996, J. Immunol., 157, 4277, 10.4049/jimmunol.157.10.4277 · DOI · Paper page
  59. Osaka F., 1998, Genes Dev., 12, 2263, 10.1101/gad.12.15.2263 · DOI · Paper page
  60. Peters J.M., 1996, Science, 274, 1199, 10.1126/science.274.5290.1199 · DOI
  61. Saitoh H., 1997, Trends Biochem. Sci., 22, 374, 10.1016/S0968-0004(97)01102-X · DOI
  62. Scheffner M., 1993, Cell, 75, 495, 10.1016/0092-8674(93)90384-3 · DOI · Paper page
  63. Scheffner M., 1995, Nature, 373, 81, 10.1038/373081a0 · DOI · Paper page
  64. Schlumpberger M., 1997, J. Bacteriol., 179, 1068, 10.1128/jb.179.4.1068-1076.1997 · DOI
  65. Schwarz S.E., 1998, Proc. Natl. Acad. Sci. USA, 95, 560, 10.1073/pnas.95.2.560 · DOI
  66. Scott S.V., 1996, Proc. Natl. Acad. Sci. USA, 93, 12304, 10.1073/pnas.93.22.12304 · DOI · Paper page
  67. Seglen P.O., 1992, Experientia, 48, 158, 10.1007/BF01923509 · DOI · Paper page
  68. Shen Z., 1996, Genomics, 37, 183, 10.1006/geno.1996.0540 · DOI
  69. Sikorski R.S., 1989, Genetics, 122, 19, 10.1093/genetics/122.1.19 · DOI · Paper page
  70. Straub M., 1997, J. Bacteriol., 197, 3875, 10.1128/jb.179.12.3875-3883.1997 · DOI
  71. Takeshige K., 1992, J. Cell Biol., 119, 301, 10.1083/jcb.119.2.301 · DOI · Paper page
  72. Thompson J.D., 1994, Nucleic Acids Res., 22, 4673, 10.1093/nar/22.22.4673 · DOI · Paper page
  73. Thumm M., 1994, FEBS Lett., 349, 275, 10.1016/0014-5793(94)00672-5 · DOI · Paper page
  74. Tsukada M., 1993, FEBS Lett., 333, 169, 10.1016/0014-5793(93)80398-E · DOI · Paper page
  75. Varshavsky A., 1997, Trends Biochem. Sci., 22, 383, 10.1016/S0968-0004(97)01122-5 · DOI · Paper page
  76. Wierenga R.K., 1983, Nature, 302, 842, 10.1038/302842a0 · DOI · Paper page
  77. Yuan W., 1999, Mol. Biol. Cell., 10, 000, 10.1091/mbc.10.5.1353 · DOI
  78. Zachariae W., 1996, Science, 274, 1201, 10.1126/science.274.5290.1201 · DOI · Paper page

Cited by

Metadata

Authors
Tanida, I., Mizushima, N., Kiyooka, M., Ohsumi, M., Ueno, T., Ohsumi, Y., & Kominami, E.
Venue
Molecular Biology of the Cell
Publisher
American Society for Cell Biology (ASCB)
Published
1999-05
Volume
10
Issue
5
Pages
1367-1379
ISSN
  • 1059-1524 (ISSN-L)
  • 1939-4586
DOI
10.1091/mbc.10.5.1367
OpenAlex
W2135954438
Cited by
417
References
0
FWCI
14.03
Type
journal article
Language
en
Open access
green

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

Data sources

Bibliographic data: Semantic Scholar, OpenAlex, Crossref.

Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.