The DOI matches its Crossref record; 57 of the 57 references it cites were found in a registry or an index.
β1-Integrin Orients Epithelial Polarity via Rac1 and Laminin
Open accessAbstract
Epithelial cells polarize and orient polarity in response to cell-cell and cell-matrix adhesion. Although there has been much recent progress in understanding the general polarizing machinery of epithelia, it is largely unclear how this machinery is controlled by the extracellular environment. To explore the signals from cell-matrix interactions that control orientation of cell polarity, we have used three-dimensional culture systems in which Madin-Darby canine kidney (MDCK) cells form polarized, lumen-containing structures. We show that interaction of collagen I with apical β1-integrins after collagen overlay of a polarized MDCK monolayer induces activation of Rac1, which is required for collagen overlay-induced tubulocyst formation. Cysts, comprised of a monolayer enclosing a central lumen, form after embedding single cells in collagen. In those cultures, addition of a β1-integrin function-blocking antibody to the collagen matrix gives rise to cysts that have defects in the organization of laminin into the basement membrane and have inverted polarity. Normal polarity is restored by either expression of activated Rac1, or the inclusion of excess laminin-1 (LN-1). Together, our results suggest a signaling pathway in which the activation of β1-integrins orients the apical pole of polarized cysts via a mechanism that requires Rac1 activation and laminin organization into the basement membrane.
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Cites
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RHO GTPASES: Biochemistry and Biology
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Self-citationSpatial organization of the extracellular matrix regulates cell–cell junction positioning
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Controlling Epithelial Polarity: A Human Enteroid Model for Host-Pathogen Interactions
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Metadata
- Authors
- Yu, W., Datta, A., Leroy, P., O'Brien, L. E., Mak, G., Jou, T.-S., Matlin, K. S., Mostov, K. E., & Zegers, M. M. P.
- Venue
- Molecular Biology of the Cell
- Published
- 2005-02
- Volume
- 16
- Issue
- 2
- Pages
- 433-445
- ISSN
- 1059-1524 (ISSN-L)
- 1939-4586
- OpenAlex
- W2330820039
- Cited by
- 361
- References
- 57
- FWCI
- 5.65
- Type
- journal article
- Language
- en
- Open access
- green
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- Yu, W., Datta, A., Leroy, P., O'Brien, L. E., Mak, G., Jou, T.-S., Matlin, K. S., Mostov, K. E., & Zegers, M. M. P. (2005). β1-Integrin Orients Epithelial Polarity via Rac1 and Laminin. Molecular Biology of the Cell. https://doi.org/10.1091/mbc.e04-05-0435
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