Sncg,Mybpc1, and Parm1 Classify subpopulations of VIP-expressing interneurons in layers 2/3 of the somatosensory cortex
Abstract
Neocortical vasoactive intestinal polypeptide-expressing (VIP+) interneurons display highly diverse morpho-electrophysiological and molecular properties. To begin to understand the function of VIP+ interneurons in cortical circuits, they must be clearly and comprehensively classified into distinct subpopulations based on specific molecular markers. Here, we utilized patch-clamp RT-PCR (Patch-PCR) to simultaneously obtain the morpho-electric properties and mRNA profiles of 155 VIP+ interneurons in layers 2 and 3 (L2/3) of the mouse somatosensory cortex. Using an unsupervised clustering method, we identified 3 electrophysiological types (E-types) and 2 morphological types (M-types) of VIP+ interneurons. Joint clustering based on the combined electrophysiological and morphological features resulted in 3 morpho-electric types (ME-types). More importantly, we found these 3 ME-types expressed distinct marker genes: ~94% of Sncg+ cells were ME-type 1, 100% of Mybpc1+ cells were ME-type 2, and ~78% of Parm1+ were ME-type 3. By clarifying the properties of subpopulations of cortical L2/3 VIP+ interneurons, this study establishes a basis for future investigations aiming to elucidate their physiological roles.
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Mybpc1 is a molecular marker for a subset of VIP interneurons.
“This gene is a molecular marker for a subset of vasoactive intestinal polypeptide-expressing (VIP) interneurons [32].”
Mybpc1 is a molecular marker for a subpopulation of vasoactive intestinal polypeptide-expressing (VIP) interneurons in the cortex.
“Mybpc1 is a molecular marker for a subpopulation of vasoactive intestinal polypeptide-expressing (VIP) interneurons in the cortex [32].”
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Milewski, T. M., Lee, W., Young, R. L., Hofmann, H. A., & Curley, J. P. (2025) · PLoS Genetics · doi:10.1371/journal.pgen.1011548 · Cited by 7 · see citing works
- Authors
- Jiang, S.-N., Cao, J.-W., Liu, L.-Y., Zhou, Y., Shan, G.-Y., Fu, Y.-H., Shao, Y.-C., & Yu, Y.-C.
- Affiliations
- Fudan University (CN)
- Fudan University School of Clinical Medicine, , Dong'an Road 131, Shanghai 200032 , China
- Fudan University Orthopaedic Department of Zhongshan Hospital, , Fenglin Road 180, Shanghai 200032 , China
- Corresponding author
- Yu, Yong-Chun
- Venue
- Cerebral Cortex
- Publisher
- Oxford University Press (OUP)
- Published
- 2022-08-27
- Volume
- 33
- Issue
- 8
- Pages
- 4293-4304
- ISSN
- 1047-3211 (ISSN-L)
- 1460-2199
- OpenAlex
- W4293410904
- Cited by
- 10
- References
- 16
- Citations by year
- 2026: 2 · 2025: 5 · 2024: 2 · 2023: 1
- FWCI
- 0.88
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model (vor)
- Locations
- Cerebral Cortex (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Collaborative Innovation Program of Shanghai Municipal Health Commission (2020CXJQ01)
- Shanghai Municipal Education Commission (2019-01-07-00-07-E00062)
- Shanghai Municipal Science and Technology Commission (2018SHZDZX01)
- National Natural Science Foundation of China (31725012, 31930044)
- National Key Research and Development Program of China (2021ZD0202500)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuropeptides and Animal Physiology
- Life Sciences › Neuroscience › Cognitive Neuroscience › Neural dynamics and brain function
- Keywords
- vasoactive intestinal peptide, somatosensory cortex, layer 2/3, interneuron subpopulations, SNCG, MYBPC1, marker genes, unsupervised clustering, mice
- MeSH terms
- Myosin Binding Protein C, Androgen-Binding Protein, Animals, Interneurons, Neoplasm Proteins, Somatosensory Cortex, Vasoactive Intestinal Peptide, Mice, gamma-Synuclein, Electrophysiological Phenomena
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